TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115079.2.1
(636 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AH014393.1|SEG_AY643842S Hordeum vulgare subsp. vulgare ... 83 1e-024
gb|AY643843.1|AY643842S2 Hordeum vulgare subsp. vulgare clo... 83 1e-024
>gb|AH014393.1|SEG_AY643842S Hordeum vulgare subsp. vulgare clones BAC 519K7, 799C8, and 122A5
hardness locus region
Length = 302994
Score = 82.6 bits (174), Expect(2) = 1e-024
Identities = 34/66 (51%), Positives = 48/66 (72%)
Frame = -2 / -3
Query: 563 FNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYLRDELK 384
++ +FWAVHPGGPAIL ++E L L KL+ SR AL ++GN SSNTI YVLE + +E +
Sbjct: 138184 YDKMFWAVHPGGPAILTKMEGRLGLDGGKLRASRSALRDFGNASSNTIVYVLENMVEESR 138005
Query: 383 KGAISE 366
+ ++E
Sbjct: 138004 RQRMTE 137987
Score = 49.6 bits (102), Expect(2) = 1e-024
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / -3
Query: 365 EWGLILAFGPGITFEGLLVRGVK 297
EWGLILAFGPGITFEG+L R ++
Sbjct: 137911 EWGLILAFGPGITFEGILARNLQ 137843
>gb|AY643843.1|AY643842S2 Hordeum vulgare subsp. vulgare clones BAC 519K7 and 799C8 hardness locus
region
Length = 129099
Score = 82.6 bits (174), Expect(2) = 1e-024
Identities = 34/66 (51%), Positives = 48/66 (72%)
Frame = -2 / -2
Query: 563 FNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYLRDELK 384
++ +FWAVHPGGPAIL ++E L L KL+ SR AL ++GN SSNTI YVLE + +E +
Sbjct: 125186 YDKMFWAVHPGGPAILTKMEGRLGLDGGKLRASRSALRDFGNASSNTIVYVLENMVEESR 125007
Query: 383 KGAISE 366
+ ++E
Sbjct: 125006 RQRMTE 124989
Score = 49.6 bits (102), Expect(2) = 1e-024
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / -2
Query: 365 EWGLILAFGPGITFEGLLVRGVK 297
EWGLILAFGPGITFEG+L R ++
Sbjct: 124913 EWGLILAFGPGITFEGILARNLQ 124845
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 288,071,452
Number of Sequences: 312970
Number of extensions: 4052018
Number of successful extensions: 36273
Number of sequences better than 1.0e-020: 2
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6786673440
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)