TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5532690.2.1
(863 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816394.1|CN816394 HRO4520_C03_F06ZS5 Lib AA071E1X Aven... 221 2e-058
gb|CN818726.1|CN818726 HRO4468_A11_A22ZS5 Lib AA070E1X Aven... 107 2e-024
>gb|CN816394.1|CN816394 HRO4520_C03_F06ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_C03_F06, mRNA sequence
Length = 591
Score = 221 bits (476), Expect = 2e-058
Identities = 91/138 (65%), Positives = 114/138 (82%)
Frame = -1 / +1
Query: 563 SLGIISRKPAYTRRDGLDFVKTEDIASCEALVFSVHVAVSNHGSMDGSHAVLLFARSKSS 384
S +ISRKPAYTRRDGLD+++ E+IASCE+L FSV+V+V+N G+MDGSHAVLLF RSKS
Sbjct: 19 SPSLISRKPAYTRRDGLDYIQVEEIASCESLTFSVYVSVTNGGTMDGSHAVLLFVRSKSG 198
Query: 383 VPGFPIKQLVGFERVHTAAGSASSVEITVDPCKHMSAANPEGKRVLLLGAHVLTVGDEEF 204
PGFP+KQL+ FERV+TAAGS+++VEI+VDPCK MSAAN EG+RVLLLG H L VGDEE
Sbjct: 199 APGFPLKQLIAFERVYTAAGSSTNVEISVDPCKLMSAANTEGRRVLLLGTHHLMVGDEEH 378
Query: 203 ELSIEL*QGCAKSYHLWS 150
E +E *+ + H ++
Sbjct: 379 EFVVEA*EATSTCLHQYT 432
Score = 168 bits (361), Expect = 1e-042
Identities = 78/116 (67%), Positives = 86/116 (74%)
Frame = +1 / -2
Query: 217 PTVRTWAPNRSTLLPSGLAALICLQGSTVISTLDALPAAVWTRSKPTSCFIGKPGTLDFD 396
PT++ W P RSTLLPS LAALI LQGST+ISTL LPAAV+TRS +CF G PG DFD
Sbjct: 365 PTIK*WVPRRSTLLPSVLAALISLQGSTLISTLVELPAAVYTRSNAINCFRGNPGAPDFD 186
Query: 397 LANRRTA*LPSMLP*FETAT*TENTRASHDAISSVFTKSSPSLLVYAGFLLIMPND 564
L RTA LPSM+P F T T*TEN R SHDAISS + SSPSL VYAGFLL+ D
Sbjct: 185 LTKSRTAWLPSMVPPFVTET*TENVRDSHDAISSTWM*SSPSLRVYAGFLLMRLGD 18
Score = 121 bits (259), Expect = 1e-028
Identities = 58/128 (45%), Positives = 78/128 (60%)
Frame = +3 / -1
Query: 177 SLSEFNG*FELFITHR*NMGTQ*KYPFAFRIGCAHMLAGVNSNLHTGCTASRRVDTLKAN 356
SL F+ LFI MG+Q K+P +F +GC H LA + ++LH G TAS V LK N
Sbjct: 405 SLLCFDDKLMLFIPDHQMMGSQEKHPSSFSVGCTHKLARIYTDLHIG*TASSCVHALKRN 226
Query: 357 QLFYRKARDA*FRSREQKNSMTPIHATMIRNSDVNREYQSFT*RDIFSFHKV*SIPPCVC 536
QL R+ A FRS +++NSM PIH + + DV+ + F *RD+F+ V* +PPCVC
Sbjct: 225 QLL*REPWSARFRSHKEQNSMAPIHGAAVCHRDVD*KC*GFA*RDLFNLDVV*PVPPCVC 46
Query: 537 RLSADNAQ 560
RL D A+
Sbjct: 45 RLPTDEAR 22
Score = 112 bits (240), Expect = 6e-026
Identities = 54/121 (44%), Positives = 67/121 (55%)
Frame = +2 / -3
Query: 179 PVRVQWIIRTLHHPPLEHGHPIEVPFCLQDWLRSYACRGQQ*SPHWMHCQPPCGHAQSQP 358
P+ ++ LH P G P E PF LQ WL S AC+ *SPHW++CQ C AQ+Q
Sbjct: 403 PLMLRRQTHALHPRPSNDGFPGEAPFFLQCWLHS*ACKDLH*SPHWLNCQQLCTRAQTQS 224
Query: 359 AVL*ESPGRLISISRTEEQHDSHPCYHDSKQRREQRIPELHMTRYLQFSQSLVHPSLCMQ 538
L + R ISIS+ EQH SHP RR ++ + MTR LQ SL PS+CMQ
Sbjct: 223 TALEGTLERPISISQRAEQHGSHPWCRRLSPRRRLKMLGIRMTRSLQPGCSLARPSVCMQ 44
Query: 539 A 541
A
Sbjct: 43 A 41
Score = 97.7 bits (207), Expect = 2e-021
Identities = 55/117 (47%), Positives = 67/117 (57%)
Frame = -2 / +2
Query: 556 ALSAESLHTQGGMD*TL*KLKISRHVKLWYSLFTSLFRIMVAWMGVMLFFCSRDRNQASR 377
A S SLHT GG *T +LK SRH + TS + WMG MLF DRN+A +
Sbjct: 26 ASSVGSLHTHGGTG*TTSRLKRSRHANP*HFQSTSR*QTAAPWMGAMLFCSL*DRNRALQ 205
Query: 376 AFL*NSWLALSVSTRRLAVHPVWRLLLTPASI*AQPILKAKGYFYWVPMF*RWVMKS 206
L*+S L LS T+ LAV P+WR + AS+* QP LK +G F W P+ * MKS
Sbjct: 206 GSL*SS*LRLSACTQLLAVQPMWRSV*ILASL*VQPTLKEEGCFSWEPII*WSGMKS 376
>gb|CN818726.1|CN818726 HRO4468_A11_A22ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4468_A11_A22, mRNA sequence
Length = 528
Score = 107 bits (229), Expect = 2e-024
Identities = 45/107 (42%), Positives = 63/107 (58%)
Frame = -1 / +2
Query: 509 FVKTEDIASCEALVFSVHVAVSNHGSMDGSHAVLLFARSKSSVPGFPIKQLVGFERVHTA 330
+V+ + CE L F V V NHG +DG H+VL+F R + G P+ QLVGF H
Sbjct: 23 YVEEIGVDGCEQLKFPAMVEVHNHGPLDGKHSVLMFLRWPNVTGGRPVSQLVGFRSQHLM 202
Query: 329 AGSASSVEITVDPCKHMSAANPEGKRVLLLGAHVLTVGDEEFELSIE 189
AG +S+ V PC+H S A +GK+V+ G+H L V +E E+S+E
Sbjct: 203 AGEKASLRFDVSPCEHFSRAREDGKKVIDRGSHFLMVDKDESEISME 343
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,982,818
Number of Sequences: 8143
Number of extensions: 173677
Number of successful extensions: 1265
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 297030472
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)