BLASTN 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 5532690.2.1
         (863 letters)

Database: Populus_nucl_with_EST.fasta 
           369,679 sequences; 203,408,664 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CK099366.1|CK099366  A064P56.5pR Hybrid aspen plasmid lib...    50   1e-004
gb|AI164515.1|AI164515  A064P56U Hybrid aspen plasmid librar...    42   0.035

>gb|CK099366.1|CK099366 A064P56.5pR Hybrid aspen plasmid library Populus tremula x Populus
           tremuloides cDNA clone A064P56 5', mRNA sequence
          Length = 523

 Score = 50.1 bits (25), Expect = 1e-004
 Identities = 62/73 (84%), Gaps = 1/73 (1%)
 Strand = Plus / Minus

                                                                       
Query: 648 tccagtgtaaa-atcgatatgttctcccagggtagcctcgtgaaggatcagctctcatgt 706
           ||||||||||| | ||||| ||||  || ||||| || || ||||||| || ||||||||
Sbjct: 396 tccagtgtaaacaccgataggttcgtcctgggtatccacgagaaggattaggtctcatgt 337

                        
Query: 707 tcatatcagtcat 719
           |||||||||||||
Sbjct: 336 tcatatcagtcat 324



>gb|AI164515.1|AI164515 A064P56U Hybrid aspen plasmid library Populus tremula x Populus
           tremuloides cDNA 5', mRNA sequence
          Length = 392

 Score = 42.1 bits (21), Expect = 0.035
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                
Query: 699 tctcatgttcatatcagtcat 719
           |||||||||||||||||||||
Sbjct: 296 tctcatgttcatatcagtcat 276



  Database: Populus_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:49 PM
  Number of letters in database: 203,408,664
  Number of sequences in database:  369,679
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 93,166
Number of Sequences: 369679
Number of extensions: 93166
Number of successful extensions: 23421
Number of sequences better than  0.5: 2
Number of HSP's better than  0.5 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 23417
Number of HSP's gapped (non-prelim): 4
length of query: 863
length of database: 203,408,664
effective HSP length: 19
effective length of query: 844
effective length of database: 196,384,763
effective search space: 165748739972
effective search space used: 165748739972
T: 0
A: 0
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)