Eucalyptus species, renowned for their fast growth, valuable wood properties and wide adaptability, are amongst the most planted hardwoods in the world but also still at the early stages of domestication because conventional breeding is slow and costly [1]. Thus, there is a huge potential in developing marker-assisted breeding programs most particularly to improve traits such as wood properties.
As a first step towards this end, we have performed the sequencing, assembly, analysis and annotation a large collection of ESTs originating from one normalized differentiating xylem cDNA library [XylcDNA] and four subtractive [SSH] libraries: [Xl] xylem versus leaves[2] ; [Xp], xylem versus phloem [3], [Mj] mature wood versus juvenile wood, [Jm] juvenile versus mature wood [4]. We named "EUCAWOOD" this dataset of 3928 wood-related unigenes (2479 contigs and 1449 singletons).
This comprehensive list of unigenes dedicated to wood formation in the genus Eucalyptus will be instrumental for candidate gene approaches, custom array development [5] and marker-assisted selection programs aimed at improving and modulating wood properties.
To mine this valuable resource, we have created the EUCAWOOD database which allows four types of query:

- Query 1 is a library filter query allowing retrieval of all unigenes or a selection of them from the user-specified libraries. EST assembly, Blasts hits, GO and PFAM annotations can also be retrieved.
- Query 2 retrieves unigenes by name (aliases), PFAM or GO annotations, hits in Blasts (accession number or name).
- Query 3 allows performing Blast of the user-specified sequence against Eucawood database.
- Query 4 give accesses to a treeview showing the number of unigenes by GO terms.

The list and sequence of 641 putative EST-SSRs can be retrieved as a pdf file.

The cDNA clones are stored at the CNRGV [] and can be supplied upon request to Hélène Berges []

[1] Myburg AA, Potts BM, Marques CMP, Kirst M, Gion J-M, Grattapaglia D, Grima-Pettenati, J. 2007. Eucalyptus. In: Kole CR, Ed. Genome Mapping & Molecular Breeding in Plants. Vol. 7: Forest Trees. Springer, Heidelberg. ISBN: 978-3-540-34540-4
[2] Paux E, Tamasloukht M, Ladouce N, Sivadon P, Grima-Pettenati J: Identification of genes preferentially expressed during wood formation in Eucalyptus. Plant Mol Biol 2004, 55(2):263-280.
[3] Foucart C, Paux E, Ladouce N, San Clemente H, Grima-Pettenati J, Sivadon P: Transcript profiling of a xylem vs phloem cDNA subtractive library identifies new genes expressed during xylogenesis in Eucalyptus. New Phytol 2006, 170(4):739-752.
[4] D Rengel, H San Clemente, F Servant, N Ladouce, E Paux, P Wincker, A Couloux, P Sivadon and J Grima-Pettenati. A new Eucalyptus genomic resource dedicated to wood formation: a comprehensive survey BMC Plant Biology 2009, 9:36.
[5] Paux E, Carocha V, Marques C, Mendes de Sousa A, Borralho N, Sivadon P, Grima-Pettenati J: Transcript profiling of Eucalyptus xylem genes during tension wood formation. New Phytol 2005, 167(1):89-100.

Contact: Jacqueline GRIMA-PETTENATI (

Webmaster : Hélène San Clemente (

Last update: September 22th 2008