BLASTX 2.2.6 [Apr-09-2003]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL3a04.xg.2.1
(369 letters)
Database: nr
3,454,138 sequences; 1,185,965,366 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_912547.1| Putative C-4 sterol methyl oxidase [Oryza ... 59 4e-08
gb|AAM65428.1| putative C-4 sterol methyl oxidase [Arabidop... 58 9e-08
gb|AAK61361.1| putative sterol 4-alpha-methyl-oxidase [Arab... 58 9e-08
ref|NP_567670.1| SMO1-2 (STEROL C4-METHYL OXIDASE); catalyt... 58 9e-08
ref|NP_192948.1| SMO1-1 (STEROL-4ALPHA-METHYL OXIDASE 1-1);... 58 9e-08
gb|AAM64961.1| putative C-4 sterol methyl oxidase [Arabidop... 58 9e-08
gb|AAQ13424.1| sterol-4-methyl-oxidase [Arabidopsis thaliana] 58 9e-08
ref|NP_567669.1| SMO1-3 (STEROL 4-ALPHA METHYL OXIDASE); ca... 57 1e-07
ref|NP_922592.1| putative C-4 sterol methyl oxidase [Oryza ... 57 2e-07
emb|CAB79230.1| predicted protein [Arabidopsis thaliana] >g... 56 3e-07
emb|CAB78254.1| putative C-4 sterol methyl oxidase [Arabido... 52 7e-06
gb|AAQ94118.1| sterol-4-alpha methyl oxidase [Arabidopsis t... 49 3e-05
dbj|BAC57961.1| putative C-4 sterol methyl oxidase [Aster t... 44 0.001
gb|AAO13795.1| putative sterol 4-alpha-methyl-oxidase [Goss... 37 0.22
gb|EAR95475.1| Sterol desaturase family protein [Tetrahymen... 36 0.38
ref|ZP_01080506.1| Helicase-like [Synechococcus sp. RS9917]... 34 1.1
ref|NP_850133.1| SMO2-1; C-4 methylsterol oxidase [Arabidop... 33 1.9
ref|NP_973559.1| SMO2-1; C-4 methylsterol oxidase [Arabidop... 33 1.9
gb|AAL32287.1| sterol 4-alpha-methyl-oxidase [Arabidopsis t... 33 1.9
ref|NP_565681.1| SMO2-1; C-4 methylsterol oxidase [Arabidop... 33 1.9
gb|AAM64821.1| putative C-4 sterol methyl oxidase [Arabidop... 33 2.4
ref|NP_563789.1| SMO2-2; C-4 methylsterol oxidase [Arabidop... 33 2.4
ref|NP_973777.1| SMO2-2; C-4 methylsterol oxidase [Arabidop... 33 2.4
gb|AAF79571.1| F22G5.23 [Arabidopsis thaliana] 33 2.4
ref|NP_701112.1| hypothetical protein PF11_0252 [Plasmodium... 33 3.2
ref|XP_638492.1| hypothetical protein DDB0186090 [Dictyoste... 31 9.2
>ref|NP_912547.1| Putative C-4 sterol methyl oxidase [Oryza sativa (japonica
cultivar-group)]
gb|AAN62786.1| Putative C-4 sterol methyl oxidase [Oryza sativa (japonica
cultivar-group)]
Length = 284
Score = 58.9 bits (141), Expect = 4e-08
Identities = 23/35 (65%), Positives = 32/35 (91%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELK 106
G ++SNFSSVFT+CDYIYGT++GY YHK SL++++
Sbjct: 245 GHSQSNFSSVFTFCDYIYGTDRGYRYHKASLSKMR 279
>gb|AAM65428.1| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
Length = 299
Score = 57.8 bits (138), Expect = 9e-08
Identities = 23/37 (62%), Positives = 32/37 (86%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXK 112
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEK 284
>gb|AAK61361.1| putative sterol 4-alpha-methyl-oxidase [Arabidopsis thaliana]
Length = 299
Score = 57.8 bits (138), Expect = 9e-08
Identities = 23/37 (62%), Positives = 32/37 (86%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXK 112
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEK 284
>ref|NP_567670.1| SMO1-2 (STEROL C4-METHYL OXIDASE); catalytic [Arabidopsis thaliana]
Length = 299
Score = 57.8 bits (138), Expect = 9e-08
Identities = 23/37 (62%), Positives = 32/37 (86%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXK 112
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEK 284
>ref|NP_192948.1| SMO1-1 (STEROL-4ALPHA-METHYL OXIDASE 1-1); catalytic [Arabidopsis
thaliana]
gb|AAN18115.1| At4g12110/F16J13_180 [Arabidopsis thaliana]
gb|AAM78091.1| AT4g12110/F16J13_180 [Arabidopsis thaliana]
Length = 298
Score = 57.8 bits (138), Expect = 9e-08
Identities = 25/46 (54%), Positives = 36/46 (78%), Gaps = 3/46 (6%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELK---XKEAEHSG 130
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K +H+G
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNKHNG 293
>gb|AAM64961.1| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
Length = 298
Score = 57.8 bits (138), Expect = 9e-08
Identities = 25/46 (54%), Positives = 36/46 (78%), Gaps = 3/46 (6%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELK---XKEAEHSG 130
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K +H+G
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNKHNG 293
>gb|AAQ13424.1| sterol-4-methyl-oxidase [Arabidopsis thaliana]
Length = 298
Score = 57.8 bits (138), Expect = 9e-08
Identities = 25/46 (54%), Positives = 36/46 (78%), Gaps = 3/46 (6%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELK---XKEAEHSG 130
G+++SNF+SVFTYCDYIYGT+KGY + K+ L ++K K +H+G
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSNKHNG 293
>ref|NP_567669.1| SMO1-3 (STEROL 4-ALPHA METHYL OXIDASE); catalytic [Arabidopsis
thaliana]
Length = 291
Score = 57.4 bits (137), Expect = 1e-07
Identities = 25/40 (62%), Positives = 32/40 (80%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXKEAE 121
G+++SNF+SVFTYCDYIYGT+KGY HK+ L +EAE
Sbjct: 244 GQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283
>ref|NP_922592.1| putative C-4 sterol methyl oxidase [Oryza sativa (japonica
cultivar-group)]
gb|AAK20047.1| putative C-4 sterol methyl oxidase [Oryza sativa (japonica
cultivar-group)]
gb|AAP54879.1| C-4 sterol methyl oxidase, putative [Oryza sativa (japonica
cultivar-group)]
Length = 301
Score = 57.0 bits (136), Expect = 2e-07
Identities = 29/57 (50%), Positives = 39/57 (68%)
Frame = +2
Query: 5 KTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXKEAEHSGKED*DNVLSTFYARI 175
+++SNF+SVFTYCDY+YGT+KGY YHK A++K K D N LS YA++
Sbjct: 247 QSQSNFASVFTYCDYLYGTDKGYRYHKAYQAKMKALGQTEGEKAD-SNGLS--YAKL 300
>emb|CAB79230.1| predicted protein [Arabidopsis thaliana]
emb|CAA16560.1| predicted protein [Arabidopsis thaliana]
Length = 820
Score = 56.2 bits (134), Expect = 3e-07
Identities = 23/37 (62%), Positives = 31/37 (83%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGYMYHKRSLAELKXK 112
G+++SNF+SVFTYCDYIYGT+KGY + K+ L + K K
Sbjct: 258 GQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQKKVK 294
Score = 44.3 bits (103), Expect = 0.001
Identities = 17/24 (70%), Positives = 23/24 (95%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNKGY 73
G+++SNF+SVFTYCDYIYGT+K +
Sbjct: 463 GQSQSNFASVFTYCDYIYGTDKRF 486
>emb|CAB78254.1| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
emb|CAB40952.1| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
Length = 303
Score = 51.6 bits (122), Expect = 7e-06
Identities = 25/51 (49%), Positives = 36/51 (70%), Gaps = 8/51 (15%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYIYGTNK-----GYMYHKRSLAELK---XKEAEHSG 130
G+++SNF+SVFTYCDYIYGT+K GY + K+ L ++K K +H+G
Sbjct: 248 GQSQSNFASVFTYCDYIYGTDKMWLYQGYRFQKKLLEQIKESSKKSNKHNG 298
>gb|AAQ94118.1| sterol-4-alpha methyl oxidase [Arabidopsis thaliana]
Length = 273
Score = 49.3 bits (116), Expect = 3e-05
Identities = 21/30 (70%), Positives = 27/30 (90%), Gaps = 1/30 (3%)
Frame = +2
Query: 2 GKTRSNFSSVFTYCDYI-YGTNKGYMYHKR 88
G+++SNF+SVFTYCDYI YGT+KGY HK+
Sbjct: 244 GQSQSNFASVFTYCDYISYGTDKGYRIHKK 273
>dbj|BAC57961.1| putative C-4 sterol methyl oxidase [Aster tripolium]
Length = 297
Score = 44.3 bits (103), Expect = 0.001
Identities = 19/33 (57%), Positives = 26/33 (78%), Gaps = 1/33 (3%)
Frame = +2
Query: 5 KTRSNF-SSVFTYCDYIYGTNKGYMYHKRSLAE 100
K++SNF +FTYCDY+YGT+KGY K+ LA+
Sbjct: 247 KSQSNFWHPIFTYCDYLYGTDKGYRNRKKLLAQ 279
>gb|AAO13795.1| putative sterol 4-alpha-methyl-oxidase [Gossypium arboreum]
Length = 269
Score = 36.6 bits (83), Expect = 0.22
Identities = 18/40 (45%), Positives = 23/40 (57%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELKXKEAEHSGKE 136
N+SS F Y D+I+GT+KGY R L LK E K+
Sbjct: 233 NYSSTFVYMDWIFGTDKGY----RKLKALKRDGVEEEAKQ 268
>gb|EAR95475.1| Sterol desaturase family protein [Tetrahymena thermophila SB210]
Length = 242
Score = 35.8 bits (81), Expect = 0.38
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +2
Query: 5 KTRSNFSSVFTYCDYIYGTNKGYMYHKRS 91
K NFSS FTY D I+ TNK Y+ K+S
Sbjct: 192 KNMGNFSSFFTYLDSIFKTNKDYLLFKQS 220
>ref|ZP_01080506.1| Helicase-like [Synechococcus sp. RS9917]
gb|EAQ68796.1| Helicase-like [Synechococcus sp. RS9917]
Length = 1179
Score = 34.3 bits (77), Expect = 1.1
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +2
Query: 257 QQIYNSPYVYYTTGFVVPHVYPQ 325
+Q+ P V+YTTGF+VPHV+ Q
Sbjct: 32 EQLSQPPSVWYTTGFLVPHVFQQ 54
>ref|NP_850133.1| SMO2-1; C-4 methylsterol oxidase [Arabidopsis thaliana]
gb|AAO50658.1| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
gb|AAO22608.1| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
gb|AAL32302.1| sterol 4-alpha-methyl-oxidase [Arabidopsis thaliana]
Length = 260
Score = 33.5 bits (75), Expect = 1.9
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELK 106
N+SS F Y D+I+GT+KGY R L LK
Sbjct: 233 NYSSTFVYMDWIFGTDKGY----RKLKALK 258
>ref|NP_973559.1| SMO2-1; C-4 methylsterol oxidase [Arabidopsis thaliana]
Length = 253
Score = 33.5 bits (75), Expect = 1.9
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELK 106
N+SS F Y D+I+GT+KGY R L LK
Sbjct: 226 NYSSTFVYMDWIFGTDKGY----RKLKALK 251
>gb|AAL32287.1| sterol 4-alpha-methyl-oxidase [Arabidopsis thaliana]
Length = 239
Score = 33.5 bits (75), Expect = 1.9
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELK 106
N+SS F Y D+I+GT+KGY R L LK
Sbjct: 212 NYSSTFVYMDWIFGTDKGY----RKLKALK 237
>ref|NP_565681.1| SMO2-1; C-4 methylsterol oxidase [Arabidopsis thaliana]
gb|AAC95199.2| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
gb|AAM64359.1| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
Length = 272
Score = 33.5 bits (75), Expect = 1.9
Identities = 16/30 (53%), Positives = 20/30 (66%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGYMYHKRSLAELK 106
N+SS F Y D+I+GT+KGY R L LK
Sbjct: 245 NYSSTFVYMDWIFGTDKGY----RKLKALK 270
>gb|AAM64821.1| putative C-4 sterol methyl oxidase [Arabidopsis thaliana]
Length = 266
Score = 33.1 bits (74), Expect = 2.4
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGY 73
N+SS F Y D+I+GT+KGY
Sbjct: 233 NYSSTFVYMDWIFGTDKGY 251
>ref|NP_563789.1| SMO2-2; C-4 methylsterol oxidase [Arabidopsis thaliana]
gb|AAL32303.1| sterol 4-alpha-methyl-oxidase [Arabidopsis thaliana]
Length = 266
Score = 33.1 bits (74), Expect = 2.4
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGY 73
N+SS F Y D+I+GT+KGY
Sbjct: 233 NYSSTFVYMDWIFGTDKGY 251
>ref|NP_973777.1| SMO2-2; C-4 methylsterol oxidase [Arabidopsis thaliana]
Length = 228
Score = 33.1 bits (74), Expect = 2.4
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGY 73
N+SS F Y D+I+GT+KGY
Sbjct: 195 NYSSTFVYMDWIFGTDKGY 213
>gb|AAF79571.1| F22G5.23 [Arabidopsis thaliana]
Length = 261
Score = 33.1 bits (74), Expect = 2.4
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +2
Query: 17 NFSSVFTYCDYIYGTNKGY 73
N+SS F Y D+I+GT+KGY
Sbjct: 228 NYSSTFVYMDWIFGTDKGY 246
>ref|NP_701112.1| hypothetical protein PF11_0252 [Plasmodium falciparum 3D7]
gb|AAN35836.1| hypothetical protein [Plasmodium falciparum 3D7]
Length = 1653
Score = 32.7 bits (73), Expect = 3.2
Identities = 18/64 (28%), Positives = 31/64 (48%)
Frame = +2
Query: 41 CDYIYGTNKGYMYHKRSLAELKXKEAEHSGKED*DNVLSTFYARISWILHKASIIYSSCS 220
C + Y T+ + L E K + E+ K+ D ++ R +W LH+A +SSC
Sbjct: 1045 CPFPYNTSSEKQ--RNILVESKNLKYENINKKTKDEEYCDYFKRYNWTLHRAE-TFSSCK 1101
Query: 221 LRSV 232
+R +
Sbjct: 1102 IRGI 1105
>ref|XP_638492.1| hypothetical protein DDB0186090 [Dictyostelium discoideum]
gb|EAL65138.1| hypothetical protein DDB0186090 [Dictyostelium discoideum]
Length = 542
Score = 31.2 bits (69), Expect = 9.2
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Frame = +2
Query: 209 SSCSLRSVPLPRD*D-VQQIYNSPYVYYTTGFVVPHVYPQSTXI 337
SS S SVP P +Q + N+P + +GF P V+PQS+ +
Sbjct: 117 SSSSSPSVPSPSTSQAIQDLMNTPLPHKRSGFTHPTVFPQSSSL 160
Database: nr
Posted date: Apr 6, 2006 2:41 PM
Number of letters in database: 1,185,965,366
Number of sequences in database: 3,454,138
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 805,858,973
Number of Sequences: 3454138
Number of extensions: 14652288
Number of successful extensions: 32912
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 32320
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 32909
length of database: 1,185,965,366
effective HSP length: 98
effective length of database: 847,459,842
effective search space used: 20339036208
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)