BLASTX 2.2.6 [Apr-09-2003] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= QCL35a05.yg.2.1 (567 letters) Database: /db/trembl-ebi/tmp/swall 1,165,242 sequences; 374,381,506 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sptr|Q8S566|Q8S566 Guanine nucleotide-exchange protein GEP1. 155 4e-37 sw|Q42510|EM30_ARATH Pattern formation protein EMB30. 148 4e-35 sptr|Q9FLY5|Q9FLY5 Pattern formation protein. 114 7e-25 sw|Q9R1D7|GBF1_CRIGR Golgi-specific brefeldin A-resistance guani... 57 1e-07 sptrnew|EAA00837|EAA00837 EbiP9614 (Fragment). 56 2e-07 sw|Q92538|GBF1_HUMAN Golgi-specific brefeldin A-resistance guani... 56 3e-07 sptr|Q9XTF0|Q9XTF0 C24H11.7 protein. 51 7e-06 sptr|Q8ML25|Q8ML25 CG8487-PA. 49 5e-05 sptr|Q9V696|Q9V696 CG8487 protein. 49 5e-05 sptr|Q8LN59|Q8LN59 Putative apical-basal pattern formation protein. 40 0.022 sptr|Q9VJW1|Q9VJW1 CG7578 protein (BG:DS00797.7 protein) (LD2917... 35 0.42 sptr|Q8IP64|Q8IP64 CG7578-PB. 35 0.42 sptrnew|EAA14874|EAA14874 ENSANGP00000006433 (Fragment). 35 0.55 sptr|Q8S565|Q8S565 Guanine nucleotide-exchange protein GEP2. 34 0.94 sptrnew|EAA33457|EAA33457 Hypothetical protein. 32 3.6 sw|Q9UT02|SC71_SCHPO Protein transport protein sec71. 32 4.7 sptr|Q96X17|Q96X17 Sec7p. 32 6.1
>sptr|Q8S566|Q8S566 Guanine nucleotide-exchange protein GEP1. Length = 1175 Score = 155 bits (391), Expect = 4e-37 Identities = 79/161 (49%), Positives = 105/161 (65%), Gaps = 3/161 (1%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296 A++VV E VL C+DG L VAK++ +H V+L KFTTLLNTS +++P Sbjct: 528 AIAVVFDHSEHEEVLLACVDGFLGVAKISAFHHLEDVLDDLVVSLCKFTTLLNTSLVEEP 587 Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRLXXXXXXXX 116 V FG+D KAR+ATE +FTIA YGD+IR+GWRN++DCIL HK+GLLP R+ Sbjct: 588 VTAFGDDLKARLATETLFTIANRYGDYIRTGWRNVLDCILRLHKLGLLPARVASDAADDS 647 Query: 115 XXXXXSLPGK--HTSSAPQVLPI-STPRKTYGLMGRFSQLL 2 ++ GK H+S + +P+ TPRK+ GLMGRFSQLL Sbjct: 648 EVSAETVQGKPTHSSISTSHIPVMGTPRKSSGLMGRFSQLL 688 >sw|Q42510|EM30_ARATH Pattern formation protein EMB30. Length = 1451 Score = 148 bits (373), Expect = 4e-35 Identities = 77/161 (47%), Positives = 101/161 (62%), Gaps = 3/161 (1%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296 A+SVV E V CIDG L++AK++ +H V+L KFTTLLN S +D+P Sbjct: 800 AISVVFDHAEHEDVYQTCIDGFLAIAKISACHHLEDVLDDLVVSLCKFTTLLNPSSVDEP 859 Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRLXXXXXXXX 116 V+ FG+D KARMAT +FTIA YGD+IR+GWRNI+DCIL HK+GLLP R+ Sbjct: 860 VLAFGDDAKARMATITIFTIANKYGDYIRTGWRNILDCILRLHKLGLLPARVASDAADES 919 Query: 115 XXXXXSLPGK---HTSSAPQVLPISTPRKTYGLMGRFSQLL 2 GK ++ S+ + + TPR++ GLMGRFSQLL Sbjct: 920 EHSSEQGQGKPLANSLSSAHLQSMGTPRRSSGLMGRFSQLL 960 >sptr|Q9FLY5|Q9FLY5 Pattern formation protein. Length = 1443 Score = 114 bits (285), Expect = 7e-25 Identities = 63/159 (39%), Positives = 86/159 (54%), Gaps = 5/159 (3%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296 A SVV E VL CIDG L++AKL+ YH V+L KFT D+ Sbjct: 800 ATSVVFEQAEQEDVLRRCIDGLLAIAKLSAYYHLNSVLDDLVVSLCKFTPFFAPLSADEA 859 Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRLXXXXXXXX 116 V+V GED +ARMATEAVF IA YGD+I +GW+NI++C+L +K+ +LP + Sbjct: 860 VLVLGEDARARMATEAVFLIANKYGDYISAGWKNILECVLSLNKLHILPDHIASDAADDP 919 Query: 115 XXXXXSLPGKHTSSAP-----QVLPISTPRKTYGLMGRF 14 +L + S+ P Q P + PRK+ +GRF Sbjct: 920 ELSTSNLEQEKPSANPVPVVSQSQPSAMPRKSSSFIGRF 958 >sw|Q9R1D7|GBF1_CRIGR Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (BFA-resistant GEF 1). Length = 1856 Score = 57.0 bits (136), Expect = 1e-07 Identities = 34/109 (31%), Positives = 55/109 (50%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296 A+S V ++ I G A ++ Y ++L KFT L + S I++ Sbjct: 935 ALSYVFDKSIEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSES-IENL 993 Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLP 149 VFG + KA +A + VF +A +GD +R GW+NI++ +L + LLP Sbjct: 994 PTVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAVLQLFRAQLLP 1042 >sptrnew|EAA00837|EAA00837 EbiP9614 (Fragment). Length = 1285 Score = 56.2 bits (134), Expect = 2e-07 Identities = 32/98 (32%), Positives = 51/98 (52%), Gaps = 4/98 (4%) Frame = -3 Query: 421 IDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLL----NTSYIDDPVIVFGEDTKARMAT 254 I G + A +A + + L KFTTLL N ++ ++FG++ KA++A Sbjct: 725 IGGFMKSAAIAAHFQLHGDFDALVLTLCKFTTLLTPPPNDAHEITASVMFGQNVKAQLAM 784 Query: 253 EAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRL 140 VF + +GD +R GWR+ +D +L K+ LLP L Sbjct: 785 RTVFGLIHEHGDCMREGWRHTMDVLLQLFKLKLLPKAL 822 >sw|Q92538|GBF1_HUMAN Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (BFA-resistant GEF 1). Length = 1859 Score = 55.8 bits (133), Expect = 3e-07 Identities = 34/109 (31%), Positives = 55/109 (50%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296 A+S V ++ I G A ++ Y ++L KFT L + S I++ Sbjct: 937 ALSYVFDKSLEETIIQKAISGFRKCAMISAHYGLSDVFDNLIISLCKFTALSSES-IENL 995 Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLP 149 VFG + KA +A + VF +A +GD +R GW+NI++ +L + LLP Sbjct: 996 PSVFGSNPKAHIAAKTVFHLAHRHGDILREGWKNIMEAMLQLFRAQLLP 1044 >sptr|Q9XTF0|Q9XTF0 C24H11.7 protein. Length = 1820 Score = 51.2 bits (121), Expect = 7e-06 Identities = 44/180 (24%), Positives = 67/180 (37%), Gaps = 24/180 (13%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNT------ 314 A+S V E +L + G AK+A Y + L KFTTL + Sbjct: 897 ALSYVFDKSEHEQILQKALTGYRKCAKIAAYYGMKEVFDNLCIHLCKFTTLTSMRDGGAG 956 Query: 313 ---------------SYIDDP---VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNII 188 S+ P + FGE+ KA++AT +F + G+ +R GWRN+ Sbjct: 957 GGADEDVDLSAAALLSHSSSPEAVALAFGENHKAQLATRTLFYLVHENGNILREGWRNLF 1016 Query: 187 DCILXXHKIGLLPGRLXXXXXXXXXXXXXSLPGKHTSSAPQVLPISTPRKTYGLMGRFSQ 8 + +L + LLP L ++ H P S +GL G S+ Sbjct: 1017 EALLQLFRARLLPAELTEVEDYVDEKGWVNIQRVHQKELPHTRNDSGLLSWFGLGGGASE 1076 >sptr|Q8ML25|Q8ML25 CG8487-PA. Length = 1740 Score = 48.5 bits (114), Expect = 5e-05 Identities = 30/101 (29%), Positives = 47/101 (46%), Gaps = 9/101 (8%) Frame = -3 Query: 415 GXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDPV---------IVFGEDTKAR 263 G A ++ Y+ + L KFTTLL++ +P + FG + KA+ Sbjct: 902 GFSKSAAISAHYNLHSDFDALVLTLCKFTTLLSSVEQHEPAPANNETQQAVNFGLNGKAQ 961 Query: 262 MATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRL 140 A VF + YGD +R W++I+D L ++ LLP L Sbjct: 962 AAMRTVFLLVHDYGDCLRESWKHILDLYLQLFRLKLLPKSL 1002 >sptr|Q9V696|Q9V696 CG8487 protein. Length = 1983 Score = 48.5 bits (114), Expect = 5e-05 Identities = 30/101 (29%), Positives = 47/101 (46%), Gaps = 9/101 (8%) Frame = -3 Query: 415 GXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDPV---------IVFGEDTKAR 263 G A ++ Y+ + L KFTTLL++ +P + FG + KA+ Sbjct: 902 GFSKSAAISAHYNLHSDFDALVLTLCKFTTLLSSVEQHEPAPANNETQQAVNFGLNGKAQ 961 Query: 262 MATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRL 140 A VF + YGD +R W++I+D L ++ LLP L Sbjct: 962 AAMRTVFLLVHDYGDCLRESWKHILDLYLQLFRLKLLPKSL 1002 >sptr|Q8LN59|Q8LN59 Putative apical-basal pattern formation protein. Length = 351 Score = 39.7 bits (91), Expect = 0.022 Identities = 22/78 (28%), Positives = 36/78 (46%), Gaps = 7/78 (8%) Frame = -3 Query: 214 IRSGWRNIIDCILXXHKIGLLPGRL--XXXXXXXXXXXXXSLPGKHTSSAPQVLPIS--- 50 +R+ W+N++DC+L + LLP + L + S + + P S Sbjct: 13 VRAAWKNVVDCLLKLKRFKLLPPSVVDQDGGASTVSSSTERLGHRAKSESGVIFPSSHRG 72 Query: 49 --TPRKTYGLMGRFSQLL 2 T R +G++GRFSQ L Sbjct: 73 AGTSRHVFGMIGRFSQFL 90 >sptr|Q9VJW1|Q9VJW1 CG7578 protein (BG:DS00797.7 protein) (LD29171p). Length = 1653 Score = 35.4 bits (80), Expect = 0.42 Identities = 24/99 (24%), Positives = 42/99 (42%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296 A SV + + T C+DG ++A +H AL++FT L S I++ Sbjct: 845 AFSVGLQDCDDPEIATLCLDGIRCAIRIACIFHMSLERDAYVQALARFTLLNANSPINE- 903 Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCI 179 K + + +A G+++ S W +I+ CI Sbjct: 904 -----MKAKNIDTIKTLIMVAHTDGNYLGSSWLDIVKCI 937 >sptr|Q8IP64|Q8IP64 CG7578-PB. Length = 1614 Score = 35.4 bits (80), Expect = 0.42 Identities = 24/99 (24%), Positives = 42/99 (42%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296 A SV + + T C+DG ++A +H AL++FT L S I++ Sbjct: 845 AFSVGLQDCDDPEIATLCLDGIRCAIRIACIFHMSLERDAYVQALARFTLLNANSPINE- 903 Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCI 179 K + + +A G+++ S W +I+ CI Sbjct: 904 -----MKAKNIDTIKTLIMVAHTDGNYLGSSWLDIVKCI 937 >sptrnew|EAA14874|EAA14874 ENSANGP00000006433 (Fragment). Length = 1522 Score = 35.0 bits (79), Expect = 0.55 Identities = 23/99 (23%), Positives = 42/99 (42%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296 A SV + + + C+DG ++A +H AL++FT L S I++ Sbjct: 765 AFSVGLQDCDDPEIASLCLDGIRCAVRIACIFHMSLERDAYVQALARFTLLTANSPINE- 823 Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCI 179 K + + +A G+++ S W +I+ CI Sbjct: 824 -----MKAKNIDTIKTLIMVAHTDGNYLGSSWLDIVKCI 857 >sptr|Q8S565|Q8S565 Guanine nucleotide-exchange protein GEP2. Length = 1789 Score = 34.3 bits (77), Expect = 0.94 Identities = 23/108 (21%), Positives = 45/108 (41%) Frame = -3 Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296 A SV + V++ C++G S + +L+KFT+L + + I Sbjct: 906 AFSVPLDQSDDEIVISQCLEGFRSAIHVTAAMSMKTQRDAFVTSLAKFTSLHSAADIKQ- 964 Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLL 152 K A +A+ IA G++++ W +I+ C+ + L+ Sbjct: 965 --------KNIEAIKAILLIADEDGNYLQEAWEHILTCVSRFENLHLV 1004 >sptrnew|EAA33457|EAA33457 Hypothetical protein. Length = 1626 Score = 32.3 bits (72), Expect = 3.6 Identities = 15/57 (26%), Positives = 28/57 (49%) Frame = -3 Query: 319 NTSYIDDPVIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLP 149 N+ + + + FG D + ++AT +F + T IR+ W+ I+ L L+P Sbjct: 977 NSVMVSELAVKFGRDVRPQLATLVLFRVVTGSEHIIRNSWKYIVRIWLNLFVNSLIP 1033 >sw|Q9UT02|SC71_SCHPO Protein transport protein sec71. Length = 1811 Score = 32.0 bits (71), Expect = 4.7 Identities = 18/64 (28%), Positives = 32/64 (50%) Frame = -3 Query: 343 LSKFTTLLNTSYIDDPVIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHK 164 L+KFT L NTS + A + + I+ A+GD +R W++++ CI + Sbjct: 1026 LTKFTHLNNTSELK---------WTNMHALKTLLEISLAHGDKLRDSWKDVLLCISQLER 1076 Query: 163 IGLL 152 + L+ Sbjct: 1077 VQLI 1080 >sptr|Q96X17|Q96X17 Sec7p. Length = 1772 Score = 31.6 bits (70), Expect = 6.1 Identities = 23/91 (25%), Positives = 33/91 (36%) Frame = -3 Query: 424 CIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDPVIVFGEDTKARMATEAV 245 CI G L+ + AL +F L N I K A A+ Sbjct: 966 CIKGIKLAIHLSCTFDIENARSSFVGALVQFGNLHNVEEISP---------KNVDAIHAL 1016 Query: 244 FTIATAYGDHIRSGWRNIIDCILXXHKIGLL 152 +A GDH+R W+ I+ + +I LL Sbjct: 1017 LNVAVTEGDHLRGSWKEILLSVSQIERIQLL 1047 Database: /db/trembl-ebi/tmp/swall Posted date: Jul 11, 2003 8:27 PM Number of letters in database: 374,381,506 Number of sequences in database: 1,165,242 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 266,493,408 Number of Sequences: 1165242 Number of extensions: 4329629 Number of successful extensions: 7373 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 7299 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 7362 length of database: 374,381,506 effective HSP length: 115 effective length of database: 240,378,676 effective search space used: 17547643348 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
S1: 41 (21.7 bits)