BLASTX 2.2.6 [Apr-09-2003] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= QAF31c07.yg.2.1 (459 letters) Database: /db/trembl-ebi/tmp/swall 1,165,242 sequences; 374,381,506 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sptr|Q9LGY7|Q9LGY7 EST AU091748(C11808) corresponds to a region ... 97 6e-35 sptr|Q9C9Z9|Q9C9Z9 Hypothetical protein (Elongation defective 1). 75 3e-27 sptr|Q8VXD5|Q8VXD5 SDL-1 protein. 73 9e-26 sptr|O80820|O80820 At2g41450 protein. 70 2e-24 sptr|Q9M2M7|Q9M2M7 Hypothetical 49.7 kDa protein. 60 7e-09 sptr|Q8LBQ8|Q8LBQ8 Hypothetical protein. 34 0.54 sptr|Q9C915|Q9C915 Hypothetical 38.7 kDa protein. 34 0.54 sptr|Q87A68|Q87A68 Protein-L-isoaspartate O-methyltransferase. 32 2.7 sptr|Q9FNI5|Q9FNI5 Gb|AAC80581.1. 32 3.5 sptr|Q9LHR7|Q9LHR7 Genomic DNA, chromosome 5, TAC clone:K3D20. 32 3.5 sptr|Q9PAD3|Q9PAD3 Protein-L-isoaspartate O-methyltransferase. 31 6.0 sptr|Q8NA29|Q8NA29 Hypothetical protein FLJ35904. 31 6.0 sptr|Q9STM1|Q9STM1 Hypothetical 43.4 kDa protein. 31 6.0 sptr|Q96F59|Q96F59 Hypothetical protein FLJ90702. 31 6.0 sptr|O44705|O44705 Hypothetical 33.3 kDa protein. 30 7.8 sptr|Q9C837|Q9C837 Betaine aldehyde dehydrogenase, putative. 30 7.8 sptr|Q9BMG3|Q9BMG3 Isoleucine tRNA synthetase (EC 6.1.1.5). 30 7.8
>sptr|Q9LGY7|Q9LGY7 EST AU091748(C11808) corresponds to a region of the predicted gene. Length = 556 Score = 97.4 bits (241), Expect(2) = 6e-35 Identities = 46/51 (90%), Positives = 48/51 (94%) Frame = +2 Query: 107 EICITGSTSAGLHQILPWLYYHNVIGVSHFFLLVEGEAAKPAXTSVLESIQ 259 +ICITGSTSAGLHQILPWLYYH VIGVSHFFL VEGEAAKPA TSVLESI+ Sbjct: 139 KICITGSTSAGLHQILPWLYYHKVIGVSHFFLFVEGEAAKPAVTSVLESIR 189 Score = 70.9 bits (172), Expect(2) = 6e-35 Identities = 37/61 (60%), Positives = 37/61 (60%) Frame = +3 Query: 258 SRIWNETXXAGXFYKPXXYELXXXQXLNMEMXIIMXRDARMDXIXXXXTDXXIXXXXXRE 437 SRIWNET AG FYKP YEL Q LNMEM IIM RDA MD I TD I RE Sbjct: 207 SRIWNETWLAGFFYKPCNYELFVKQSLNMEMAIIMARDAGMDWIIHLDTDELIHPAGARE 266 Query: 438 Y 440 Y Sbjct: 267 Y 267 Score = 55.5 bits (132), Expect(2) = 3e-08 Identities = 26/27 (96%), Positives = 27/27 (100%) Frame = +3 Query: 3 SLRRLLLDVPDNVDMVIFPNYESSIDR 83 SLRRLLLDVPDNVDMVIFPNYESSI+R Sbjct: 268 SLRRLLLDVPDNVDMVIFPNYESSIER 294 Score = 22.7 bits (47), Expect(2) = 3e-08 Identities = 8/9 (88%), Positives = 9/9 (100%) Frame = +2 Query: 86 DIKDPFTEI 112 DIKDPFTE+ Sbjct: 296 DIKDPFTEV 304 >sptr|Q9C9Z9|Q9C9Z9 Hypothetical protein (Elongation defective 1). Length = 533 Score = 75.5 bits (184), Expect(2) = 3e-27 Identities = 35/50 (70%), Positives = 42/50 (84%) Frame = +2 Query: 107 EICITGSTSAGLHQILPWLYYHNVIGVSHFFLLVEGEAAKPAXTSVLESI 256 +ICIT STSAGL QILPW++YH V+GVS FFL VEG+AA P+ + VLESI Sbjct: 129 KICITTSTSAGLDQILPWMFYHKVLGVSTFFLFVEGKAATPSISKVLESI 178 Score = 67.0 bits (162), Expect(2) = 3e-27 Identities = 34/61 (55%), Positives = 36/61 (59%) Frame = +3 Query: 258 SRIWNETXXAGXFYKPXXYELXXXQXLNMEMXIIMXRDARMDXIXXXXTDXXIXXXXXRE 437 SRIWNET + FYKP YEL Q LNMEM I+M RDA MD I TD I RE Sbjct: 197 SRIWNETWLSSFFYKPCNYELFVKQSLNMEMAIVMARDAGMDWILHLDTDELIYPAGARE 256 Query: 438 Y 440 Y Sbjct: 257 Y 257 Score = 52.4 bits (124), Expect(2) = 2e-07 Identities = 24/27 (88%), Positives = 26/27 (96%) Frame = +3 Query: 3 SLRRLLLDVPDNVDMVIFPNYESSIDR 83 SLRRLLLDVP NVDMVIFPNYESS++R Sbjct: 258 SLRRLLLDVPPNVDMVIFPNYESSVER 284 Score = 22.7 bits (47), Expect(2) = 2e-07 Identities = 8/9 (88%), Positives = 9/9 (100%) Frame = +2 Query: 86 DIKDPFTEI 112 DIKDPFTE+ Sbjct: 286 DIKDPFTEV 294 >sptr|Q8VXD5|Q8VXD5 SDL-1 protein. Length = 529 Score = 72.8 bits (177), Expect(2) = 9e-26 Identities = 34/50 (68%), Positives = 40/50 (80%) Frame = +2 Query: 107 EICITGSTSAGLHQILPWLYYHNVIGVSHFFLLVEGEAAKPAXTSVLESI 256 +ICIT STSAGL QILPW++YH VIGV+ F L VEG+AA P + VLESI Sbjct: 116 KICITTSTSAGLEQILPWMFYHKVIGVTSFLLFVEGKAASPDVSKVLESI 165 Score = 64.7 bits (156), Expect(2) = 9e-26 Identities = 32/61 (52%), Positives = 35/61 (57%) Frame = +3 Query: 258 SRIWNETXXAGXFYKPXXYELXXXQXLNMEMXIIMXRDARMDXIXXXXTDXXIXXXXXRE 437 SRIWNET + FYKP YEL Q LNMEM I+M RD MD I TD + RE Sbjct: 184 SRIWNETWLSSFFYKPCNYELFVKQSLNMEMAIVMARDGGMDWIIHLDTDELLHPAGARE 243 Query: 438 Y 440 Y Sbjct: 244 Y 244 Score = 51.2 bits (121), Expect(2) = 5e-07 Identities = 23/27 (85%), Positives = 26/27 (96%) Frame = +3 Query: 3 SLRRLLLDVPDNVDMVIFPNYESSIDR 83 SLR+LLLDVP NVDMVIFPNYESS++R Sbjct: 245 SLRQLLLDVPSNVDMVIFPNYESSVER 271 Score = 22.7 bits (47), Expect(2) = 5e-07 Identities = 8/9 (88%), Positives = 9/9 (100%) Frame = +2 Query: 86 DIKDPFTEI 112 DIKDPFTE+ Sbjct: 273 DIKDPFTEV 281 >sptr|O80820|O80820 At2g41450 protein. Length = 991 Score = 70.1 bits (170), Expect(2) = 2e-24 Identities = 39/69 (56%), Positives = 45/69 (65%) Frame = +2 Query: 50 YLPQL*KQH*PYDIKDPFTEICITGSTSAGLHQILPWLYYHNVIGVSHFFLLVEGEAAKP 229 YL L H P D+K ICIT STSAGL Q LPW++YH VIGV F+L VEG AA P Sbjct: 655 YLRDLKLDHKP-DLKP---RICITTSTSAGLEQTLPWIFYHKVIGVETFYLFVEGTAASP 710 Query: 230 AXTSVLESI 256 + VLE+I Sbjct: 711 NVSRVLETI 719 Score = 63.2 bits (152), Expect(2) = 2e-24 Identities = 33/61 (54%), Positives = 34/61 (55%) Frame = +3 Query: 258 SRIWNETXXAGXFYKPXXYELXXXQXLNMEMXIIMXRDARMDXIXXXXTDXXIXXXXXRE 437 SRIWNET FYKP YEL Q LNMEM I M RDA MD I TD + RE Sbjct: 738 SRIWNETWLEKFFYKPCNYELFVKQNLNMEMAITMARDAGMDWILHLDTDELVHPSGTRE 797 Query: 438 Y 440 Y Sbjct: 798 Y 798 Score = 40.0 bits (92), Expect(2) = 0.002 Identities = 19/27 (70%), Positives = 22/27 (81%) Frame = +3 Query: 3 SLRRLLLDVPDNVDMVIFPNYESSIDR 83 SLR LL DVP +VD VIF NYESS++R Sbjct: 799 SLRNLLRDVPADVDEVIFTNYESSVER 825 Score = 21.2 bits (43), Expect(2) = 0.002 Identities = 7/9 (77%), Positives = 9/9 (100%) Frame = +2 Query: 86 DIKDPFTEI 112 DIK+PFTE+ Sbjct: 827 DIKEPFTEV 835 >sptr|Q9M2M7|Q9M2M7 Hypothetical 49.7 kDa protein. Length = 439 Score = 60.5 bits (145), Expect = 7e-09 Identities = 36/84 (42%), Positives = 41/84 (48%), Gaps = 4/84 (4%) Frame = +3 Query: 201 Y*LKERLQSQLXPLFLNL----FSRIWNETXXAGXFYKPXXYELXXXQXLNMEMXIIMXR 368 Y KE + Q F L + RIWNET + FYKP YEL Q LNMEM I M + Sbjct: 137 YRTKELEEKQAKRYFSGLHLCGYCRIWNETWLSSFFYKPCNYELFVKQSLNMEMAITMAQ 196 Query: 369 DARMDXIXXXXTDXXIXXXXXREY 440 DA M+ I TD I EY Sbjct: 197 DAGMEWIIHLDTDELIHPSGTHEY 220 >sptr|Q8LBQ8|Q8LBQ8 Hypothetical protein. Length = 346 Score = 34.3 bits (77), Expect = 0.54 Identities = 13/21 (61%), Positives = 15/21 (71%) Frame = +2 Query: 152 LPWLYYHNVIGVSHFFLLVEG 214 LPWL+YH V G HFF L +G Sbjct: 314 LPWLHYHEVAGGGHFFPLAKG 334 >sptr|Q9C915|Q9C915 Hypothetical 38.7 kDa protein. Length = 346 Score = 34.3 bits (77), Expect = 0.54 Identities = 13/21 (61%), Positives = 15/21 (71%) Frame = +2 Query: 152 LPWLYYHNVIGVSHFFLLVEG 214 LPWL+YH V G HFF L +G Sbjct: 314 LPWLHYHEVAGGGHFFPLAKG 334 >sptr|Q87A68|Q87A68 Protein-L-isoaspartate O-methyltransferase. Length = 218 Score = 32.0 bits (71), Expect = 2.7 Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 6/63 (9%) Frame = +2 Query: 101 FTEICITGSTSAGLHQILPWLYYHNVIGVSHFF------LLVEGEAAKPAXTSVLESIQP 262 F+ IC+TG+ S Q L WL+ + + H +LV G+A+ P TS+ E+ Sbjct: 146 FSVICLTGAVSTLPLQFLQWLHPNGRMFAIHGHSPAMQAVLVRGDASTPNITSLFETDLA 205 Query: 263 HLE 271 +L+ Sbjct: 206 YLK 208 >sptr|Q9FNI5|Q9FNI5 Gb|AAC80581.1. Length = 1516 Score = 31.6 bits (70), Expect = 3.5 Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 1/30 (3%) Frame = +1 Query: 136 WPTPDSSMVVLSQCHWCFTF-LSIS*RRGC 222 WP+PD + +C WC + L+ + RRGC Sbjct: 694 WPSPDKKEITRERCGWCHSCKLTSASRRGC 723 >sptr|Q9LHR7|Q9LHR7 Genomic DNA, chromosome 5, TAC clone:K3D20. Length = 1515 Score = 31.6 bits (70), Expect = 3.5 Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 1/30 (3%) Frame = +1 Query: 136 WPTPDSSMVVLSQCHWCFTF-LSIS*RRGC 222 WP+PD + +C WC + L+ + RRGC Sbjct: 690 WPSPDKKEITRERCGWCHSCRLTSASRRGC 719 >sptr|Q9PAD3|Q9PAD3 Protein-L-isoaspartate O-methyltransferase. Length = 218 Score = 30.8 bits (68), Expect = 6.0 Identities = 19/63 (30%), Positives = 33/63 (52%), Gaps = 6/63 (9%) Frame = +2 Query: 101 FTEICITGSTSAGLHQILPWLYYHNVIGVSHFF------LLVEGEAAKPAXTSVLESIQP 262 F+ IC+TG+ + Q L WL+ + + H +LV G+A+ P TS+ E+ Sbjct: 146 FSVICLTGAVNTLPLQFLQWLHPNGRMFAIHGHSPAMQAVLVRGDASTPNITSLFETDLA 205 Query: 263 HLE 271 +L+ Sbjct: 206 YLK 208 >sptr|Q8NA29|Q8NA29 Hypothetical protein FLJ35904. Length = 543 Score = 30.8 bits (68), Expect = 6.0 Identities = 12/39 (30%), Positives = 22/39 (56%) Frame = +2 Query: 89 IKDPFTEICITGSTSAGLHQILPWLYYHNVIGVSHFFLL 205 I DP +CI+ S L +++PW+ + + V +FL+ Sbjct: 108 ITDPLVGLCISKSPWTCLGRLMPWIIFSTPLAVIAYFLI 146 >sptr|Q9STM1|Q9STM1 Hypothetical 43.4 kDa protein. Length = 385 Score = 30.8 bits (68), Expect = 6.0 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = +2 Query: 152 LPWLYYHNVIGVSHFFLLVEGEAAK 226 LPW+ YH + G HF VEG K Sbjct: 341 LPWIRYHEISGSGHFVPFVEGMTDK 365 >sptr|Q96F59|Q96F59 Hypothetical protein FLJ90702. Length = 530 Score = 30.8 bits (68), Expect = 6.0 Identities = 12/39 (30%), Positives = 22/39 (56%) Frame = +2 Query: 89 IKDPFTEICITGSTSAGLHQILPWLYYHNVIGVSHFFLL 205 I DP +CI+ S L +++PW+ + + V +FL+ Sbjct: 95 ITDPLVGLCISKSPWTCLGRLMPWIIFSTPLAVIAYFLI 133 >sptr|O44705|O44705 Hypothetical 33.3 kDa protein. Length = 290 Score = 30.4 bits (67), Expect = 7.8 Identities = 19/58 (32%), Positives = 29/58 (50%), Gaps = 2/58 (3%) Frame = +3 Query: 72 SIDRMTLRILLLRSVSREA-HLLAYT-RFFHGCIITMSLVFHISFY*LKERLQSQLXP 239 SI L I LL+ + R+ HL+ + RFF I+ S+V H+ FY + + P Sbjct: 17 SIFTCVLNISLLKQIERKKKHLILFKFRFFFDVILGASVVAHLGFYIILTSFHDEFLP 74 >sptr|Q9C837|Q9C837 Betaine aldehyde dehydrogenase, putative. Length = 578 Score = 30.4 bits (67), Expect = 7.8 Identities = 14/27 (51%), Positives = 18/27 (66%) Frame = -1 Query: 150 IWCRPADVLPVIQISVKGSLMSYGQCC 70 I C ADV V Q++V+G+L S GQ C Sbjct: 306 IICEDADVSHVAQVAVRGTLQSSGQNC 332 >sptr|Q9BMG3|Q9BMG3 Isoleucine tRNA synthetase (EC 6.1.1.5). Length = 1087 Score = 30.4 bits (67), Expect = 7.8 Identities = 15/43 (34%), Positives = 22/43 (51%) Frame = +2 Query: 68 KQH*PYDIKDPFTEICITGSTSAGLHQILPWLYYHNVIGVSHF 196 ++H PY+ +D F + S GL Q W Y V+GV+ F Sbjct: 578 QEHFPYENQDKFKSLFPADFVSEGLDQTRGWFYTLLVLGVALF 620 Database: /db/trembl-ebi/tmp/swall Posted date: Jul 11, 2003 8:27 PM Number of letters in database: 374,381,506 Number of sequences in database: 1,165,242 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 213,128,862 Number of Sequences: 1165242 Number of extensions: 3463973 Number of successful extensions: 6781 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 6712 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 6781 length of database: 374,381,506 effective HSP length: 111 effective length of database: 245,039,644 effective search space used: 10046625404 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
S1: 41 (21.7 bits)