TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA17a05.yg.2.1
(548 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE498794.1|BE498794 WHE0966_G09_N18ZS Wheat pre-anthesis... 133 1e-043
gb|BE493730.1|BE493730 WHE0574_F12_L24ZE Triticum monococcu... 175 4e-043
gb|BJ265891.1|BJ265891 BJ265891 Y. Ogihara unpublished cDNA... 171 4e-042
gb|BJ260192.1|BJ260192 BJ260192 Y. Ogihara unpublished cDNA... 163 2e-039
gb|CK209599.1|CK209599 FGAS021371 Triticum aestivum FGAS: L... 158 4e-038
gb|CA593233.1|CA593233 wpa1c.pk003.d20 wpa1c Triticum aesti... 108 4e-035
gb|CD918504.1|CD918504 G608.109L01F010907 G608 Triticum aes... 133 6e-035
gb|CA706096.1|CA706096 wdk1c.pk022.h16 wdk1c Triticum aesti... 104 1e-031
gb|CD454064.1|CD454064 WHE0966_G09_N18ZT CS wheat pre-anthe... 133 1e-030
gb|CD872897.1|CD872897 AZO2.121N15F010209 AZO2 Triticum aes... 87 4e-027
gb|BJ297738.1|BJ297738 BJ297738 Y. Ogihara unpublished cDNA... 87 4e-027
gb|BJ247659.1|BJ247659 BJ247659 Y. Ogihara unpublished cDNA... 87 4e-027
gb|BF199557.1|BF199557 WHE0591-0594_O16_O16ZE Triticum mono... 94 9e-027
gb|CD872898.1|CD872898 AZO2.121N15R010521 AZO2 Triticum aes... 85 6e-026
gb|BJ253760.1|BJ253760 BJ253760 Y. Ogihara unpublished cDNA... 87 2e-024
gb|CA704545.1|CA704545 wdk1c.pk011.g17 wdk1c Triticum aesti... 111 9e-024
>gb|BE498794.1|BE498794 WHE0966_G09_N18ZS Wheat pre-anthesis spike cDNA library Triticum
aestivum cDNA clone WHE0966_G09_N18, mRNA sequence
Length = 462
Score = 133 bits (286), Expect(2) = 1e-043
Identities = 46/54 (85%), Positives = 52/54 (96%)
Frame = -3 / +2
Query: 432 SAPVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR*HEA 271
+APVQY+HSW+FCQ+GCVDNYFTAHYQSPRQMLCLW+KLS GRA CCNYR*H+A
Sbjct: 95 TAPVQYIHSWKFCQYGCVDNYFTAHYQSPRQMLCLWDKLSLGRAQCCNYR*HDA 256
Score = 108 bits (230), Expect = 6e-023
Identities = 44/54 (81%), Positives = 45/54 (83%)
Frame = +2 / -1
Query: 272 ASCYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGAL 433
ASCYL*LQ CA P DS SQRHSICLGDW*CAVK*LST P WQN QL YCTGA+
Sbjct: 255 ASCYL*LQHCARPNDSLSQRHSICLGDW*CAVK*LSTHPYWQNFQL*MYCTGAV 94
Score = 85.4 bits (180), Expect(2) = 4e-023
Identities = 39/51 (76%), Positives = 42/51 (82%)
Frame = -2 / +1
Query: 430 RPRAVCPQLEVLPVRLRGQLLHGALPVPQADAVPLGEAVSGESALL*LQIT 278
RP AV QLEVLPVR+RGQLLHGALPVPQADAVPLG+ V A+L LQIT
Sbjct: 97 RPGAVHSQLEVLPVRVRGQLLHGALPVPQADAVPLGQTVVRPRAVLQLQIT 249
Score = 62.9 bits (131), Expect(2) = 1e-043
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = -3 / +2
Query: 513 SRHTNHSLRLFKMEDVSMGMWVEDYNAS 430
SRH N SLRLFKMEDVSMGMWVEDYN +
Sbjct: 5 SRHANQSLRLFKMEDVSMGMWVEDYNTT 88
Score = 42.8 bits (87), Expect(2) = 4e-023
Identities = 17/29 (58%), Positives = 19/29 (65%)
Frame = -1 / +3
Query: 512 PGIQTTPYGCSRWRT*AWACGWRTTTPAP 426
P Q + GCSRWR *AWACG + TT P
Sbjct: 6 PVTQISH*GCSRWRM*AWACGSKITTLPP 92
>gb|BE493730.1|BE493730 WHE0574_F12_L24ZE Triticum monococcum vegetative apex cDNA library
Triticum monococcum cDNA clone WHE0574_F12_L24, mRNA
sequence
Length = 655
Score = 175 bits (376), Expect = 4e-043
Identities = 65/92 (70%), Positives = 78/92 (84%)
Frame = -3 / +1
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
VIS GIA+ V S+H N SLRLFKMEDVSMG+WVE +N + PV+Y HSW+FCQ+GC++NY+
Sbjct: 34 VISGGIAKFVVSQHANQSLRLFKMEDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLENYY 213
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR*HEA 271
TAHYQSPRQMLCLW+KL GR CCNYR* +A
Sbjct: 214 TAHYQSPRQMLCLWDKLVHGRPSCCNYR**DA 309
Score = 110 bits (235), Expect = 1e-023
Identities = 55/92 (59%), Positives = 61/92 (66%)
Frame = +2 / -3
Query: 272 ASCYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGALAL*SST 451
AS YL*LQQ P + S +HSICLGD *CAV * S QP WQN QL Y TG + L ST
Sbjct: 308 ASHYL*LQQDGRP*TNLSHKHSICLGD**CAV**FSRQPYWQNFQL*EYLTGMV*LNFST 129
Query: 452 HMPMLTSSILNSRREWFVCRDATSLAMPAEIT 547
H P+LTSSILNS + C D T+ AMP EIT
Sbjct: 128 HSPILTSSILNSLKL*LAC*DTTNFAMPPEIT 33
>gb|BJ265891.1|BJ265891 BJ265891 Y. Ogihara unpublished cDNA library, Wh_h Triticum
aestivum cDNA clone whh23o06 3', mRNA sequence
Length = 607
Score = 171 bits (369), Expect = 4e-042
Identities = 63/89 (70%), Positives = 75/89 (84%)
Frame = -3 / -2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
VIS IA+ V S+H N SLRLFKMEDVSMG+WVE +N + PV+Y HSW+FCQ+GC++NY+
Sbjct: 600 VISGSIAKFVVSQHANQSLRLFKMEDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLENYY 421
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
TAHYQSPRQMLCLW+KL GR CCNYR*
Sbjct: 420 TAHYQSPRQMLCLWDKLVRGRPSCCNYR* 334
Score = 105 bits (223), Expect = 5e-022
Identities = 52/89 (58%), Positives = 58/89 (65%)
Frame = +2 / +2
Query: 281 YL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGALAL*SSTHMP 460
YL*LQQ P + S +HSICLGD *CAV * S QP WQN QL Y TG + L STH P
Sbjct: 335 YL*LQQDGRPRTNLSHKHSICLGD**CAV**FSRQPYWQNFQL*EYLTGMV*LNFSTHSP 514
Query: 461 MLTSSILNSRREWFVCRDATSLAMPAEIT 547
+LTSSILNS + C D T+ AM EIT
Sbjct: 515 ILTSSILNSLKL*LAC*DTTNFAMLPEIT 601
>gb|BJ260192.1|BJ260192 BJ260192 Y. Ogihara unpublished cDNA library, Wh_h Triticum
aestivum cDNA clone whh23o06 5', mRNA sequence
Length = 588
Score = 163 bits (350), Expect = 2e-039
Identities = 59/85 (69%), Positives = 71/85 (83%)
Frame = -3 / +2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
VIS IA+ V S+H N SLRLFKMEDVSMG+WVE +N + PV+Y HSW+FCQ+GC++NY+
Sbjct: 332 VISGSIAKFVVSQHANQSLRLFKMEDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLENYY 511
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCC 292
TAHYQSPRQMLCLW+KL GR CC
Sbjct: 512 TAHYQSPRQMLCLWDKLVRGRPSCC 586
>gb|CK209599.1|CK209599 FGAS021371 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1130
Score = 158 bits (340), Expect = 4e-038
Identities = 59/89 (66%), Positives = 73/89 (82%)
Frame = -3 / +3
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVDNYF 367
VIS IA+ V S+H N SLRLFKMEDVSMG+WVE +N + PV+Y HSW+FCQ+GC++NY+
Sbjct: 540 VISGSIAKFVVSQHANQSLRLFKMEDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLENYY 719
Query: 366 TAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
TAHYQSPR+MLCLW+KL GR +YR*
Sbjct: 720 TAHYQSPRRMLCLWDKLVRGRPSLLHYR* 806
>gb|CA593233.1|CA593233 wpa1c.pk003.d20 wpa1c Triticum aestivum cDNA clone wpa1c.pk003.d20
5' end, mRNA sequence
Length = 575
Score = 108 bits (230), Expect(2) = 4e-035
Identities = 37/49 (75%), Positives = 44/49 (89%)
Frame = -3 / +3
Query: 426 PVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
PV+YVHS +FCQFGC+D+Y+TAHYQSPRQMLCLW+KL AG+ CCN R*
Sbjct: 183 PVEYVHSVKFCQFGCIDDYYTAHYQSPRQMLCLWDKLQAGKPRCCNMR* 329
Score = 60.2 bits (125), Expect(2) = 4e-035
Identities = 25/37 (67%), Positives = 28/37 (75%)
Frame = -3 / +2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYN 436
VISA IA + S T+H LRLFKMEDVSMGMWV +N
Sbjct: 62 VISADIADSIVSEFTDHKLRLFKMEDVSMGMWVGRFN 172
>gb|CD918504.1|CD918504 G608.109L01F010907 G608 Triticum aestivum cDNA clone G608109L01,
mRNA sequence
Length = 710
Score = 133 bits (286), Expect(2) = 6e-035
Identities = 46/54 (85%), Positives = 52/54 (96%)
Frame = -3 / +2
Query: 432 SAPVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR*HEA 271
+APVQY+HSW+FCQ+GCVDNYFTAHYQSPRQMLCLW+KLS GRA CCNYR*H+A
Sbjct: 53 TAPVQYIHSWKFCQYGCVDNYFTAHYQSPRQMLCLWDKLSLGRAQCCNYR*HDA 214
Score = 108 bits (230), Expect = 6e-023
Identities = 44/54 (81%), Positives = 45/54 (83%)
Frame = +2 / -3
Query: 272 ASCYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGAL 433
ASCYL*LQ CA P DS SQRHSICLGDW*CAVK*LST P WQN QL YCTGA+
Sbjct: 213 ASCYL*LQHCARPNDSLSQRHSICLGDW*CAVK*LSTHPYWQNFQL*MYCTGAV 52
Score = 34.1 bits (68), Expect(2) = 6e-035
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = -3 / +2
Query: 474 EDVSMGMWVEDYNAS 430
ED SMGMWVEDYN +
Sbjct: 2 EDGSMGMWVEDYNTT 46
>gb|CA706096.1|CA706096 wdk1c.pk022.h16 wdk1c Triticum aestivum cDNA clone wdk1c.pk022.h16
5' end, mRNA sequence
Length = 431
Score = 104 bits (222), Expect(2) = 1e-031
Identities = 38/57 (66%), Positives = 48/57 (84%)
Frame = -3 / +2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNASAPVQYVHSWRFCQFGCVD 376
VIS GIA+ V S+H N SLRLFKMEDVSMG+WVE +N + PV+Y HSW+FCQ+GC++
Sbjct: 170 VISGGIAKFVVSQHANQSLRLFKMEDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLE 340
Score = 51.9 bits (107), Expect(2) = 1e-031
Identities = 17/20 (85%), Positives = 20/20 (100%)
Frame = -3 / +3
Query: 375 NYFTAHYQSPRQMLCLWEKL 316
NY+TAHYQSPRQMLCLW+K+
Sbjct: 342 NYYTAHYQSPRQMLCLWDKV 401
>gb|CD454064.1|CD454064 WHE0966_G09_N18ZT CS wheat pre-anthesis spike cDNA library Triticum
aestivum cDNA clone WHE0966_G09_N18, mRNA sequence
Length = 726
Score = 133 bits (286), Expect = 1e-030
Identities = 46/54 (85%), Positives = 52/54 (96%)
Frame = -3 / -1
Query: 432 SAPVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR*HEA 271
+APVQY+HSW+FCQ+GCVDNYFTAHYQSPRQMLCLW+KLS GRA CCNYR*H+A
Sbjct: 717 TAPVQYIHSWKFCQYGCVDNYFTAHYQSPRQMLCLWDKLSLGRAQCCNYR*HDA 556
Score = 108 bits (230), Expect = 6e-023
Identities = 44/54 (81%), Positives = 45/54 (83%)
Frame = +2 / +2
Query: 272 ASCYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGAL 433
ASCYL*LQ CA P DS SQRHSICLGDW*CAVK*LST P WQN QL YCTGA+
Sbjct: 557 ASCYL*LQHCARPNDSLSQRHSICLGDW*CAVK*LSTHPYWQNFQL*MYCTGAV 718
>gb|CD872897.1|CD872897 AZO2.121N15F010209 AZO2 Triticum aestivum cDNA clone AZO2121N15,
mRNA sequence
Length = 706
Score = 87.2 bits (184), Expect(2) = 4e-027
Identities = 30/49 (61%), Positives = 37/49 (75%)
Frame = -3 / +2
Query: 426 PVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
PV+Y+H +F Q GC D Y+TAHYQSP+ M+CLW KL +G A CCN R*
Sbjct: 299 PVEYLHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR* 445
Score = 54.7 bits (113), Expect(2) = 4e-027
Identities = 22/37 (59%), Positives = 28/37 (75%)
Frame = -3 / +2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYN 436
VIS+ IA + S N +LRLFKMEDVSMGMWV+ ++
Sbjct: 176 VISSDIAEYIVSEFDNQALRLFKMEDVSMGMWVQKFS 286
>gb|BJ297738.1|BJ297738 BJ297738 Y. Ogihara unpublished cDNA library, Wh_SL Triticum
aestivum cDNA clone whsl21n21 3', mRNA sequence
Length = 646
Score = 87.2 bits (184), Expect(2) = 4e-027
Identities = 30/49 (61%), Positives = 37/49 (75%)
Frame = -3 / -2
Query: 426 PVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
PV+Y+H +F Q GC D Y+TAHYQSP+ M+CLW KL +G A CCN R*
Sbjct: 375 PVEYLHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR* 229
Score = 54.7 bits (113), Expect(2) = 4e-027
Identities = 22/37 (59%), Positives = 28/37 (75%)
Frame = -3 / -2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYN 436
VIS+ IA + S N +LRLFKMEDVSMGMWV+ ++
Sbjct: 498 VISSDIAEYIVSEFDNQALRLFKMEDVSMGMWVQKFS 388
>gb|BJ247659.1|BJ247659 BJ247659 Y. Ogihara unpublished cDNA library, Wh_f Triticum
aestivum cDNA clone whf3c24 5', mRNA sequence
Length = 589
Score = 87.2 bits (184), Expect(2) = 4e-027
Identities = 30/49 (61%), Positives = 37/49 (75%)
Frame = -3 / +2
Query: 426 PVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
PV+Y+H +F Q GC D Y+TAHYQSP+ M+CLW KL +G A CCN R*
Sbjct: 227 PVEYLHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR* 373
Score = 54.7 bits (113), Expect(2) = 4e-027
Identities = 22/37 (59%), Positives = 28/37 (75%)
Frame = -3 / +2
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYN 436
VIS+ IA + S N +LRLFKMEDVSMGMWV+ ++
Sbjct: 104 VISSDIAEYIVSEFDNQALRLFKMEDVSMGMWVQKFS 214
>gb|BF199557.1|BF199557 WHE0591-0594_O16_O16ZE Triticum monococcum vegetative apex cDNA
library Triticum monococcum cDNA clone
WHE0591-0594_O16_O16, mRNA sequence
Length = 459
Score = 93.6 bits (198), Expect(2) = 9e-027
Identities = 34/50 (68%), Positives = 37/50 (74%)
Frame = -3 / +1
Query: 423 VQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR*HE 274
V+YVH RFCQ GC D+Y TAHYQSP QMLCLWE L G CCN R*H+
Sbjct: 265 VEYVHGARFCQAGCADDYLTAHYQSPGQMLCLWENLLQGMPQCCNAR*HD 414
Score = 46.9 bits (96), Expect(2) = 9e-027
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -1 / +2
Query: 485 CSRWRT*AWACGWRTTTPA 429
C RWRT*AWACGW ++TPA
Sbjct: 206 CPRWRT*AWACGWGSSTPA 262
>gb|CD872898.1|CD872898 AZO2.121N15R010521 AZO2 Triticum aestivum cDNA clone AZO2121N15,
mRNA sequence
Length = 584
Score = 84.9 bits (179), Expect(2) = 6e-026
Identities = 29/49 (59%), Positives = 36/49 (73%)
Frame = -3 / -3
Query: 426 PVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
PV+Y+H +F Q GC D Y+TAHY SP+ M+CLW KL +G A CCN R*
Sbjct: 429 PVEYLHDVKFLQAGCFDGYYTAHYPSPQHMICLWRKLQSGSAQCCNAR* 283
Score = 52.8 bits (109), Expect(2) = 6e-026
Identities = 21/37 (56%), Positives = 28/37 (75%)
Frame = -3 / -3
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYN 436
VIS+ IA + S N +LRLF+MEDVSMGMWV+ ++
Sbjct: 552 VISSDIAEYIVSEFDNQALRLFQMEDVSMGMWVQKFS 442
>gb|BJ253760.1|BJ253760 BJ253760 Y. Ogihara unpublished cDNA library, Wh_f Triticum
aestivum cDNA clone whf3c24 3', mRNA sequence
Length = 589
Score = 87.2 bits (184), Expect(2) = 2e-024
Identities = 30/49 (61%), Positives = 37/49 (75%)
Frame = -3 / -1
Query: 426 PVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
PV+Y+H +F Q GC D Y+TAHYQSP+ M+CLW KL +G A CCN R*
Sbjct: 400 PVEYLHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR* 254
Score = 45.5 bits (93), Expect(2) = 2e-024
Identities = 17/26 (65%), Positives = 21/26 (80%)
Frame = -3 / -1
Query: 513 SRHTNHSLRLFKMEDVSMGMWVEDYN 436
S N +LRLFKMEDVSMGMWV+ ++
Sbjct: 490 SEFDNQALRLFKMEDVSMGMWVQKFS 413
>gb|CA704545.1|CA704545 wdk1c.pk011.g17 wdk1c Triticum aestivum cDNA clone wdk1c.pk011.g17
5' end, mRNA sequence
Length = 459
Score = 111 bits (236), Expect = 9e-024
Identities = 37/49 (75%), Positives = 44/49 (89%)
Frame = -3 / +3
Query: 426 PVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
PV+Y HSW+FCQ+GC++NY+TAHYQSPRQMLCLW+KL GR CCNYR*
Sbjct: 12 PVRYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGRPSCCNYR* 158
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 640,356,663
Number of Sequences: 636343
Number of extensions: 12339872
Number of successful extensions: 220426
Number of sequences better than 1.0e-020: 23
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 11612065010
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)