TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN10e11.xg.2.2
(581 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ271067.1|BJ271067 BJ271067 Y. Ogihara unpublished cDNA... 127 7e-038
gb|CN009326.1|CN009326 WHE3857_D12_H23ZS Wheat Fusarium gra... 127 8e-038
gb|CV765668.1|CV765668 FGAS060055 Triticum aestivum FGAS: L... 127 1e-037
gb|U60754.1|TAU60754 Triticum aestivum delta-24-sterol meth... 127 3e-037
gb|U60755.1|TAU60755 Triticum aestivum delta-24-sterol meth... 57 6e-028
gb|BJ225577.1|BJ225577 BJ225577 Y. Ogihara unpublished cDNA... 122 2e-027
gb|CD865738.1|CD865738 AZO2.101K09F001120 AZO2 Triticum aes... 81 3e-022
>gb|BJ271067.1|BJ271067 BJ271067 Y. Ogihara unpublished cDNA library, Wh_oh Triticum
aestivum cDNA clone whoh23p14 5', mRNA sequence
Length = 650
Score = 127 bits (272), Expect(3) = 7e-038
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGL NNDY
Sbjct: 94 RWNGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDY 273
Query: 396 HVSRGK 413
++RGK
Sbjct: 274 QITRGK 291
Score = 103 bits (219), Expect(2) = 2e-025
Identities = 43/70 (61%), Positives = 51/70 (72%)
Frame = -2 / -1
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ PL W*SLL NPV EVELNLAIS+ GPPIP PTS+TFIP + +C A KC CRLM
Sbjct: 302 RLSAFPLVIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCKARKCSCRLM 123
Query: 244 ASREVSPGYL 215
S + SP +L
Sbjct: 122 LSLKDSPFHL 93
Score = 48.7 bits (100), Expect(3) = 7e-038
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 34 YDLATSFYEYGWGESFHFA 90
Score = 34.1 bits (68), Expect(2) = 2e-025
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -1
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP PYS*K V RS*
Sbjct: 89 AKWKDSPQPYS*KLVARS* 33
Score = 21.7 bits (41), Expect(3) = 7e-038
Identities = 9/26 (34%), Positives = 11/26 (42%)
Frame = +3 / +1
Query: 504 SAXLSEXCCXLXGDXXNXXXADNTFD 581
S L C + D +DNTFD
Sbjct: 304 SVGLGATCDFVKADFMKMPFSDNTFD 381
>gb|CN009326.1|CN009326 WHE3857_D12_H23ZS Wheat Fusarium graminearum infected spike cDNA
library Triticum aestivum cDNA clone WHE3857_D12_H23,
mRNA sequence
Length = 723
Score = 127 bits (272), Expect(3) = 8e-038
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGL NNDY
Sbjct: 382 RWNGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDY 561
Query: 396 HVSRGK 413
++RGK
Sbjct: 562 QITRGK 579
Score = 101 bits (216), Expect(2) = 7e-025
Identities = 42/70 (60%), Positives = 51/70 (72%)
Frame = -2 / -2
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ PL W*SLL NPV EVELNLAIS+ GPPIP PTS+TF+P + +C A KC CRLM
Sbjct: 590 RLSAFPLVIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFMPGFNSSCKARKCSCRLM 411
Query: 244 ASREVSPGYL 215
S + SP +L
Sbjct: 410 LSLKDSPFHL 381
Score = 48.7 bits (100), Expect(3) = 8e-038
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 322 YDLATSFYEYGWGESFHFA 378
Score = 34.1 bits (68), Expect(2) = 7e-025
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -2
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP PYS*K V RS*
Sbjct: 377 AKWKDSPQPYS*KLVARS* 321
Score = 21.7 bits (41), Expect(3) = 8e-038
Identities = 9/26 (34%), Positives = 11/26 (42%)
Frame = +3 / +1
Query: 504 SAXLSEXCCXLXGDXXNXXXADNTFD 581
S L C + D +DNTFD
Sbjct: 592 SVGLGATCDFVKADFMKMPFSDNTFD 669
>gb|CV765668.1|CV765668 FGAS060055 Triticum aestivum FGAS: Library 2 Gate 3 Triticum
aestivum cDNA, mRNA sequence
Length = 816
Score = 127 bits (272), Expect(3) = 1e-037
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGL NNDY
Sbjct: 218 RWNGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDY 397
Query: 396 HVSRGK 413
++RGK
Sbjct: 398 QITRGK 415
Score = 100 bits (212), Expect(2) = 3e-024
Identities = 42/70 (60%), Positives = 50/70 (71%)
Frame = -2 / -1
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ PL W*SLL NPV EVELNLAIS+ GPPIP PTS+TFIP + +C A KC C LM
Sbjct: 426 RLSAFPLVIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCKARKCSCLLM 247
Query: 244 ASREVSPGYL 215
S + SP +L
Sbjct: 246 LSLKDSPFHL 217
Score = 48.7 bits (100), Expect(3) = 1e-037
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 158 YDLATSFYEYGWGESFHFA 214
Score = 34.1 bits (68), Expect(2) = 3e-024
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -1
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP PYS*K V RS*
Sbjct: 213 AKWKDSPQPYS*KLVARS* 157
Score = 21.7 bits (41), Expect(3) = 1e-037
Identities = 9/26 (34%), Positives = 11/26 (42%)
Frame = +3 / +2
Query: 504 SAXLSEXCCXLXGDXXNXXXADNTFD 581
S L C + D +DNTFD
Sbjct: 428 SVGLGATCDFVKADFMKMPFSDNTFD 505
>gb|U60754.1|TAU60754 Triticum aestivum delta-24-sterol methyltransferase (TA-MT) mRNA,
complete cds
Length = 1392
Score = 127 bits (272), Expect(3) = 3e-037
Identities = 51/66 (77%), Positives = 59/66 (89%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGL NNDY
Sbjct: 305 RWNGESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNDY 484
Query: 396 HVSRGK 413
++RGK
Sbjct: 485 QITRGK 502
Score = 100 bits (212), Expect(2) = 5e-024
Identities = 42/70 (60%), Positives = 50/70 (71%)
Frame = -2 / -1
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ PL W*SLL NPV EVELNLAIS+ GPPIP PTS+TFIP + +C A KC C LM
Sbjct: 513 RLSAFPLVIW*SLLFNPVTEVELNLAISLNGPPIPQPTSKTFIPGFNSSCKARKCSCLLM 334
Query: 244 ASREVSPGYL 215
S + SP +L
Sbjct: 333 LSLKDSPFHL 304
Score = 48.7 bits (100), Expect(3) = 3e-037
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 245 YDLATSFYEYGWGESFHFA 301
Score = 34.1 bits (68), Expect(2) = 5e-024
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = -2 / -1
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP PYS*K V RS*
Sbjct: 300 AKWKDSPQPYS*KLVARS* 244
Score = 21.7 bits (41), Expect(3) = 3e-037
Identities = 9/26 (34%), Positives = 11/26 (42%)
Frame = +3 / +2
Query: 504 SAXLSEXCCXLXGDXXNXXXADNTFD 581
S L C + D +DNTFD
Sbjct: 515 SVGLGATCDFVKADFMKMPFSDNTFD 592
>gb|U60755.1|TAU60755 Triticum aestivum delta-24-sterol methyltransferase (TA-MT) gene,
complete cds
Length = 4749
Score = 56.5 bits (117), Expect(4) = 6e-028
Identities = 22/32 (68%), Positives = 28/32 (87%)
Frame = +3 / +1
Query: 210 YFRYPGETSREAIKRHQHFMALQLGLKKGMKV 305
+FR+ GE+ RE+IKRH+HF+ALQL LK GMKV
Sbjct: 1228 HFRWNGESLRESIKRHEHFLALQLELKPGMKV 1323
Score = 48.7 bits (100), Expect(4) = 6e-028
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYEYGWG+SFHFA
Sbjct: 1030 YDLATSFYEYGWGESFHFA 1086
Score = 42.8 bits (87), Expect(4) = 6e-028
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = +3 / +2
Query: 303 VLDVGCGIGGPLIEIARF 356
VLDVGCGIGGPL EIARF
Sbjct: 1454 VLDVGCGIGGPLREIARF 1507
Score = 41.8 bits (85), Expect(4) = 6e-028
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = +3 / +1
Query: 357 SSTSITGLTNNDYHVSRGKVCILAFF 434
SSTS+TGL NNDY ++RGKV + F
Sbjct: 2245 SSTSVTGLNNNDYQITRGKVVQWSLF 2322
>gb|BJ225577.1|BJ225577 BJ225577 Y. Ogihara unpublished cDNA library, Wh_dL Triticum
aestivum cDNA clone whdl22m20 5', mRNA sequence
Length = 488
Score = 122 bits (260), Expect(2) = 2e-027
Identities = 49/63 (77%), Positives = 56/63 (88%)
Frame = +3 / +3
Query: 225 GETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDYHVS 404
GE+ RE+IKRH+HF+ALQL LK GMKVLDVGCGIGGPL EIARFSSTS+TGL NN Y ++
Sbjct: 6 GESLRESIKRHEHFLALQLELKPGMKVLDVGCGIGGPLREIARFSSTSVTGLNNNXYQIT 185
Query: 405 RGK 413
RGK
Sbjct: 186 RGK 194
Score = 21.7 bits (41), Expect(2) = 2e-027
Identities = 9/26 (34%), Positives = 11/26 (42%)
Frame = +3 / +3
Query: 504 SAXLSEXCCXLXGDXXNXXXADNTFD 581
S L C + D +DNTFD
Sbjct: 207 SVGLGATCDFVKADFMKMPFSDNTFD 284
>gb|CD865738.1|CD865738 AZO2.101K09F001120 AZO2 Triticum aestivum cDNA clone AZO2101K09,
mRNA sequence
Length = 690
Score = 81.3 bits (171), Expect(2) = 3e-022
Identities = 32/66 (48%), Positives = 46/66 (69%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ E+ +I RH+H++A +L L++G + LDVGCG+GGP IARFS +TGL NN Y
Sbjct: 296 RHRLESFEASIARHEHYLAWKLKLEEGQRCLDVGCGVGGPQRCIARFSRAIVTGLNNNAY 475
Query: 396 HVSRGK 413
+SR +
Sbjct: 476 QISRAR 493
Score = 46.0 bits (94), Expect(2) = 3e-022
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDLVT FYE+GWG SFHFA
Sbjct: 236 YDLVTTFYEWGWGHSFHFA 292
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 503,958,449
Number of Sequences: 636343
Number of extensions: 7315298
Number of successful extensions: 103819
Number of sequences better than 1.0e-020: 8
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 12594624357
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)