TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAW3d03.yg.2.1
(557 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF133398.1|CF133398 WHE4357_E10_J19ZT Wheat meiotic flor... 249 1e-065
gb|BG606256.1|BG606256 WHE2962_F04_L08ZS Wheat dormant embr... 224 8e-058
gb|BJ248053.1|BJ248053 BJ248053 Y. Ogihara unpublished cDNA... 215 3e-055
>gb|CF133398.1|CF133398 WHE4357_E10_J19ZT Wheat meiotic floret cDNA library Triticum
aestivum cDNA clone WHE4357_E10_J19, mRNA sequence
Length = 760
Score = 249 bits (539), Expect = 1e-065
Identities = 111/177 (62%), Positives = 116/177 (65%)
Frame = -2 / +3
Query: 532 APXVXXQEXXXXIXXXDXXXXEEXXXXXLRSXASGXXXXTRXXRLMSNHXXVVXXXPVYH 353
AP + QE I D EE LRS A+G TR RLMSNH VV PVY
Sbjct: 51 APVIYSQETVSYIEGLDMMSGEEDRENILRSRATGKAVLTRPFRLMSNHLGVVLTFPVYL 230
Query: 352 AXXXXDXXXEDRXAAXAGXXXGAFDVESLVXNXXRQLAGNQXLVVNVYDVTNISNPLVMY 173
D EDR AA AG GAFDVESLV N RQLAGNQ LVVNVYDVTN SNPLVMY
Sbjct: 231 VDLPPDAKVEDRVAATAGYLGGAFDVESLVENLLRQLAGNQELVVNVYDVTNHSNPLVMY 410
Query: 172 XSEVPLXXPSSSHICMLDFGDPFRKHHMVCRYRXXPHVPXSAISTPSGVFVICMLVG 2
SEVPL PS SH C LDFGDPFR HHM+CRYR PHVP SAI+TPSGVFVI ML+G
Sbjct: 411 GSEVPLGAPSPSHTCTLDFGDPFRNHHMICRYRSEPHVPWSAITTPSGVFVILMLIG 581
Score = 188 bits (405), Expect = 4e-047
Identities = 94/165 (56%), Positives = 101/165 (61%)
Frame = +3 / -1
Query: 3 PTSIHMTKTPDGVEIADXGT*GXXLYLXTI*CFLNGSPKSSMQMCDDEGXXRGTSDXYMT 182
P SI +T TPDGV IAD GT G LYL +* FLNGSPKSS+Q+C+ EG RGTSD YMT
Sbjct: 580 PISISITNTPDGVVIADHGTWGSLLYLHIM**FLNGSPKSSVQVCEGEGAPRGTSDPYMT 401
Query: 183 RGFEMFVTS*TFTTNX*LPASCLXXFXTNDSTSNAPXXXPAXAAXRSSXXXSXXXXA**T 362
RGFE FVTS*TFTT+ *LPASC F T+DSTS AP PA AA RSS S *T
Sbjct: 400 RGFEWFVTS*TFTTSS*LPASCRSKFSTSDSTSKAPPRYPAVAATRSSTLASGGRSTR*T 221
Query: 363 GXXXTTXX*FDMSRXXRVXXXXPDAXDLXXXXXXSSXXXXSXXSM 497
G TT FDMS V P A DL SS S SM
Sbjct: 220 GNVSTTPRWFDMSLNGLVRTAFPVALDLRMFSRSSSPDIMSSPSM 86
Score = 161 bits (346), Expect = 6e-039
Identities = 75/175 (42%), Positives = 85/175 (48%)
Frame = -1 / +2
Query: 533 RAXXXXAGXXXXHRXXXXXXXRGXXXXXXEVXSIGXXXXHSXXXAHVESPXCRXXXSCLS 354
RA G HR RG EV H AHVE P CL
Sbjct: 50 RAGHLLPGDGVLHRGARHDVRRGGPGEHPEVEGDREGRPHQAIQAHVEPPGRGTDVPCLP 229
Query: 353 CXSXX*XXXGGSXXCXXRXXXGSI*CRIISXKXXXAASWQSXIGGKCL*CHKHFEPSCHV 174
C GGS C R CR+ K AASWQS GG+CL*CHK FE SCHV
Sbjct: 230 CRPPPGCQGGGSCRCYRRISWRRFRCRVTCGKFAAAASWQSGAGGECL*CHKPFESSCHV 409
Query: 173 XIRSSSXXPLIIAHLHARFW*SIQKASYGXQIQXXASCPXVCNFYPIWCLCHMYA 9
+ S S PL + HLHA FW*SIQK+S+ QIQ A C VC +YP+WC+ + YA
Sbjct: 410 WV*SPSWCPLTLTHLHA*FW*SIQKSSHDMQIQKRAPCSMVCYYYPVWCVRYTYA 574
Score = 149 bits (320), Expect = 2e-035
Identities = 80/168 (47%), Positives = 92/168 (54%)
Frame = +2 / -3
Query: 11 HTYDKDTRWGRNCRXXDMRLXSVSAXHMMLSEWITKI*HADVR**GXXKRNF*XIHDKRV 190
H Y++ TR G N R +M L SVSA H+M+SEWITKI A V *G KR+F IHDKR+
Sbjct: 572 HKYNEHTRRGSNSRPWNMGLASVSAYHVMISEWITKIKRAGV*G*GGTKRDFRPIHDKRI 393
Query: 191 RNVCDIINIYHQXLIAS*LPXXIXX**FYIKCSPXXSCXXSXTILLXXIXXXXXMIDRXX 370
R VCDIINI+HQ LIAS*LP I * YI+ + S S TIL I +DR
Sbjct: 392 RMVCDIINIHHQLLIAS*LPQQIFHK*LYIESASKISGGSSDTILHLGIRGEVDKVDRER 213
Query: 371 XYDTXVIRHEPXXSSXXXXPRCSXPQXXXXXLLAXXXVXXXDVXXXLL 514
Y V+RHEP P PQ LLA V DV LL
Sbjct: 212 QYHAQVVRHEPEWPGEDGLPGRPRPQDVLPVLLAGHHVEPLDVGHRLL 69
Score = 126 bits (269), Expect = 3e-028
Identities = 64/129 (49%), Positives = 69/129 (53%)
Frame = +1 / -2
Query: 16 I*QRHQMG*KLQTXGHEAXXCICXPYDAF*MDHQNLACRCAMMRGXQEELLXXT*QEGSK 195
+*+ HQ G* QT H A CIC D F*MDHQN ACRC +RG QE L T*QE S
Sbjct: 567 V*RTHQTG***QTMEHGARFCICISCDDF*MDHQNQACRCVRVRGHQEGLQTHT*QEDSN 388
Query: 196 CL*HHKHLPPIXDCQLAAXXXFXLMILHQMLPXXILXXQXXDPPXXHXXXDXHDRQEXXX 375
L*HHKH PP DCQLAA F + LH+ I Q DPP H RQ
Sbjct: 387 GL*HHKHSPPAPDCQLAAAANFPQVTLHRKRLQDIRR*QRHDPPPWHPGGGRQGRQGTSV 208
Query: 376 RHXGDST*A 402
G ST*A
Sbjct: 207 PRPGGST*A 181
Score = 119 bits (254), Expect = 3e-026
Identities = 76/174 (43%), Positives = 80/174 (45%)
Frame = -3 / +1
Query: 531 RXXXXRRXXXXTSXXXTXXXARXXXXXX*GXEHRGXXXSLXXXGSCRITXVSXXXFLSIM 352
R R TS T AR *G G S GSCR T LS +
Sbjct: 52 RRSSTPRRRCPTSRGST*CPARRTGRTS*GRGRPGRPSSPGHSGSCRTTWAWY*RSLSTL 231
Query: 351 XIXXXMXXRRIVXLXXQDXXGEHLM*NH*XKXXXGS*LAIXNWW*MFMMSQTFRTLLSCX 172
M RIV L D M*+H K GS*LAI +WW*MFMMSQT R LLSC
Sbjct: 232 STSPRMPRWRIVSLLPPDILEALSM*SHLWKICCGS*LAIRSWW*MFMMSQTIRILLSCM 411
Query: 171 XQKFLLXXPHHRTSAC*ILVIHSESIIWXADTXXSLMSXXLQFLPHLVSLSYVC 10
K LL PH T A ILVIHSE I ADT S M L LP LV Y+C
Sbjct: 412 GLKSLLVPPHPHTPARLILVIHSEIIT*YADTEASPMFHGLLLLPRLVCSLYLC 573
>gb|BG606256.1|BG606256 WHE2962_F04_L08ZS Wheat dormant embryo cDNA library Triticum
aestivum cDNA clone WHE2962_F04_L08, mRNA sequence
Length = 528
Score = 224 bits (483), Expect = 8e-058
Identities = 96/133 (72%), Positives = 99/133 (74%)
Frame = -2 / +1
Query: 403 RLMSNHXXVVXXXPVYHAXXXXDXXXEDRXAAXAGXXXGAFDVESLVXNXXRQLAGNQXL 224
RLMSNH VV PVY D EDR AA AG GAFDVESLV N RQLAGNQ L
Sbjct: 7 RLMSNHLGVVLTFPVYLVDLPPDAKVEDRVAATAGYLGGAFDVESLVENLLRQLAGNQEL 186
Query: 223 VVNVYDVTNISNPLVMYXSEVPLXXPSSSHICMLDFGDPFRKHHMVCRYRXXPHVPXSAI 44
VVNVYDVTN SNPLVMY SEVPL PS SH CMLDFGDPFR HHM+CRYR PHVP SAI
Sbjct: 187 VVNVYDVTNHSNPLVMYGSEVPLGAPSPSHTCMLDFGDPFRNHHMICRYRSEPHVPWSAI 366
Query: 43 STPSGVFVICMLV 5
+TPSGVFVI ML+
Sbjct: 367 TTPSGVFVILMLI 405
Score = 181 bits (389), Expect = 7e-045
Identities = 86/131 (65%), Positives = 92/131 (70%)
Frame = +3 / -2
Query: 9 SIHMTKTPDGVEIADXGT*GXXLYLXTI*CFLNGSPKSSMQMCDDEGXXRGTSDXYMTRG 188
SI +TKTPDGV IAD GT G LYL +* FLNGSPKSSMQ+C+ EG RGTSD YMTRG
Sbjct: 401 SISITKTPDGVVIADHGTWGSLLYLHIM**FLNGSPKSSMQVCEGEGAPRGTSDPYMTRG 222
Query: 189 FEMFVTS*TFTTNX*LPASCLXXFXTNDSTSNAPXXXPAXAAXRSSXXXSXXXXA**TGX 368
FE FVTS*TFTT+ *LPASC F T+DSTS AP PA AA RSS S *TG
Sbjct: 221 FEWFVTS*TFTTSS*LPASCRNKFSTSDSTSKAPPRYPAVAATRSSTLASGGRSTR*TGN 42
Query: 369 XXTTXX*FDMS 401
TT *FDMS
Sbjct: 41 VSTTPR*FDMS 9
Score = 162 bits (348), Expect = 3e-039
Identities = 70/131 (53%), Positives = 79/131 (60%)
Frame = -1 / +3
Query: 401 AHVESPXCRXXXSCLSCXSXX*XXXGGSXXCXXRXXXGSI*CRIISXKXXXAASWQSXIG 222
AHVESP CL C GGS C R CR+ K AASWQS G
Sbjct: 9 AHVESPGRGADVPCLPCRPPPGCQGGGSCCCYRRIPWRRFRCRVTCGKFVAAASWQSGAG 188
Query: 221 GKCL*CHKHFEPSCHVXIRSSSXXPLIIAHLHARFW*SIQKASYGXQIQXXASCPXVCNF 42
G+CL*CHK F+PSCHV + S S PL +AHLHARFW*SIQK+S+ QIQ A C VC +
Sbjct: 189 GECL*CHKPFKPSCHVWV*SPSWCPLALAHLHARFW*SIQKSSHDMQIQKRAPCSMVCYY 368
Query: 41 YPIWCLCHMYA 9
YPIWCL + YA
Sbjct: 369 YPIWCLRYTYA 401
Score = 151 bits (324), Expect = 6e-036
Identities = 73/131 (55%), Positives = 84/131 (64%)
Frame = +2 / -1
Query: 11 HTYDKDTRWGRNCRXXDMRLXSVSAXHMMLSEWITKI*HADVR**GXXKRNF*XIHDKRV 190
H Y++DTRWG N R +M L SVSA H+M+SEWITKI*HA VR G KR+F IHDKRV
Sbjct: 399 HKYNEDTRWGSNSRPWNMGLASVSAYHVMISEWITKI*HAGVRGRGGTKRDFRPIHDKRV 220
Query: 191 RNVCDIINIYHQXLIAS*LPXXIXX**FYIKCSPXXSCXXSXTILLXXIXXXXXMIDRXX 370
VCDIINI+HQ LIAS*LP I * YI+ + S S TIL I +DR
Sbjct: 219 *MVCDIINIHHQLLIAS*LPQQIFHK*LYIESASKVSGGSSNTILHLGIRGEVDKVDRER 40
Query: 371 XYDTXVIRHEP 403
+ VIRHEP
Sbjct: 39 QHHAQVIRHEP 7
Score = 139 bits (298), Expect = 3e-032
Identities = 69/129 (53%), Positives = 73/129 (56%)
Frame = +1 / -3
Query: 16 I*QRHQMG*KLQTXGHEAXXCICXPYDAF*MDHQNLACRCAMMRGXQEELLXXT*QEGSK 195
+*+RHQMG* QT H A CIC D F*MDHQNLACRCA RG QE L T*QEG
Sbjct: 394 V*RRHQMG***QTMEHGARFCICISCDDF*MDHQNLACRCARARGHQEGLQTHT*QEGLN 215
Query: 196 CL*HHKHLPPIXDCQLAAXXXFXLMILHQMLPXXILXXQXXDPPXXHXXXDXHDRQEXXX 375
L*HHKH PP DCQLAA F + LH+ I Q DPP H RQ
Sbjct: 214 GL*HHKHSPPAPDCQLAAATNFPQVTLHRKRLQGIRR*QQHDPPPWHPGGGRQGRQGTSA 35
Query: 376 RHXGDST*A 402
GDST*A
Sbjct: 34 PRPGDST*A 8
Score = 127 bits (272), Expect = 1e-028
Identities = 70/131 (53%), Positives = 75/131 (57%)
Frame = -3 / +2
Query: 402 GSCRITXVSXXXFLSIMXIXXXMXXRRIVXLXXQDXXGEHLM*NH*XKXXXGS*LAIXNW 223
GSCRIT LS + M RIV L D M*+H K GS*LAI +W
Sbjct: 8 GSCRITWAWC*RSLSTLSTSPRMPRWRIVLLLPPDTLEALSM*SHLWKICCGS*LAIRSW 187
Query: 222 W*MFMMSQTFRTLLSCXXQKFLLXXPHHRTSAC*ILVIHSESIIWXADTXXSLMSXXLQF 43
W*MFMMSQT +TLLSC K LL P RT AC*ILVIHSE I ADT S M L
Sbjct: 188 W*MFMMSQTIQTLLSCMGLKSLLVPPRPRTPAC*ILVIHSEIIT*YADTEASPMFHGLLL 367
Query: 42 LPHLVSLSYVC 10
LPHLVS Y+C
Sbjct: 368 LPHLVSSLYLC 400
>gb|BJ248053.1|BJ248053 BJ248053 Y. Ogihara unpublished cDNA library, Wh_f Triticum
aestivum cDNA clone whf4l08 5', mRNA sequence
Length = 459
Score = 215 bits (464), Expect = 3e-055
Identities = 94/140 (67%), Positives = 97/140 (69%)
Frame = -2 / +1
Query: 448 LRSXASGXXXXTRXXRLMSNHXXVVXXXPVYHAXXXXDXXXEDRXAAXAGXXXGAFDVES 269
LRS A+G TR RLMSNH VV PVY D EDR AA AG GAFDV S
Sbjct: 40 LRSRATGKAVLTRPFRLMSNHLGVVLTFPVYLVDLPPDAKVEDRVAATAGYLGGAFDVXS 219
Query: 268 LVXNXXRQLAGNQXLVVNVYDVTNISNPLVMYXSEVPLXXPSSSHICMLDFGDPFRKHHM 89
LV N RQLAGNQ LVVNVYDVTN SNPL MY SEVPL PS SH C LDFGDPFR HHM
Sbjct: 220 LVENLLRQLAGNQELVVNVYDVTNHSNPLXMYGSEVPLGAPSPSHTCTLDFGDPFRNHHM 399
Query: 88 VCRYRXXPHVPXSAISTPSG 29
+CRYR PHVP SAI+TPSG
Sbjct: 400 ICRYRSEPHVPWSAITTPSG 459
Score = 165 bits (355), Expect = 3e-040
Identities = 81/139 (58%), Positives = 87/139 (62%)
Frame = +3 / -2
Query: 30 PDGVEIADXGT*GXXLYLXTI*CFLNGSPKSSMQMCDDEGXXRGTSDXYMTRGFEMFVTS 209
PDGV IAD GT G LYL +* FLNGSPKSS+Q+C+ EG RGTSD YM RGFE FVTS
Sbjct: 458 PDGVVIADHGTWGSLLYLHIM**FLNGSPKSSVQVCEGEGAPRGTSDPYMXRGFEWFVTS 279
Query: 210 *TFTTNX*LPASCLXXFXTNDSTSNAPXXXPAXAAXRSSXXXSXXXXA**TGXXXTTXX* 389
*T TT+ *LPASC F T+D TS AP PA AA RSS S *TG TT
Sbjct: 278 *TLTTSS*LPASCRNKFSTSDXTSKAPPRYPAVAATRSSTLASGGRSTR*TGNVSTTPRW 99
Query: 390 FDMSRXXRVXXXXPDAXDL 446
FDMS V P A DL
Sbjct: 98 FDMSLNGLVRTAFPVALDL 42
Score = 124 bits (265), Expect = 9e-028
Identities = 68/140 (48%), Positives = 78/140 (55%)
Frame = +2 / -1
Query: 29 TRWGRNCRXXDMRLXSVSAXHMMLSEWITKI*HADVR**GXXKRNF*XIHDKRVRNVCDI 208
TRWG N R +M L SVSA H+M+SE ITKI* A V *G KR+F IH KR+ VCDI
Sbjct: 459 TRWGSNSRPWNMGLASVSAYHVMISERITKI*RAGV*G*GGTKRDFRPIHXKRI*MVCDI 280
Query: 209 INIYHQXLIAS*LPXXIXX**FYIKCSPXXSCXXSXTILLXXIXXXXXMIDRXXXYDTXV 388
INI HQ LIAS*LP I * YI+ + S S TIL I +DR + V
Sbjct: 279 INIDHQLLIAS*LPQQIFHK*LYIESASKVSRGSSNTILHLGIRGKVDKVDRERQHHAQV 100
Query: 389 IRHEPXXSSXXXXPRCSXPQ 448
+RHEP P PQ
Sbjct: 99 VRHEPEWPREDGLPGGPGPQ 40
Score = 119 bits (254), Expect(2) = 1e-028
Identities = 47/83 (56%), Positives = 56/83 (67%)
Frame = -1 / +3
Query: 278 CRIISXKXXXAASWQSXIGGKCL*CHKHFEPSCHVXIRSSSXXPLIIAHLHARFW*SIQK 99
C + K AASWQS GG+CL*CHK F+ S HV + S S PL + HLHARFW*S+QK
Sbjct: 210 CXVTCGKFVAAASWQSGAGGQCL*CHKPFKSSXHVWV*SPSWCPLTLTHLHARFW*SVQK 389
Query: 98 ASYGXQIQXXASCPXVCNFYPIW 30
+S+ QIQ A C VC +YPIW
Sbjct: 390 SSHDMQIQKRAPCSMVCYYYPIW 458
Score = 115 bits (245), Expect = 5e-025
Identities = 60/124 (48%), Positives = 63/124 (50%)
Frame = +1 / -3
Query: 31 QMG*KLQTXGHEAXXCICXPYDAF*MDHQNLACRCAMMRGXQEELLXXT*QEGSKCL*HH 210
QMG* QT H A CIC D F* DHQNLACRC +RG QE L T QE L*HH
Sbjct: 457 QMG***QTMEHGARFCICISCDDF*TDHQNLACRCVRVRGHQEGLQTHTXQEDLNGL*HH 278
Query: 211 KHLPPIXDCQLAAXXXFXLMILHQMLPXXILXXQXXDPPXXHXXXDXHDRQEXXXRHXGD 390
KH PP DCQLAA F + H+ I Q DPP H RQ G
Sbjct: 277 KH*PPAPDCQLAAATNFPQVTXHRKRLQGIPR*QQHDPPPWHPGEGRQGRQGTSAPRPGG 98
Query: 391 ST*A 402
ST*A
Sbjct: 97 ST*A 86
Score = 27.6 bits (54), Expect(2) = 1e-028
Identities = 13/36 (36%), Positives = 13/36 (36%)
Frame = -1 / +3
Query: 416 HSXXXAHVESPXCRXXXSCLSCXSXX*XXXGGSXXC 309
H AHVE P CL C GGS C
Sbjct: 72 HEAIQAHVEPPGRGADVPCLPCRPSPGCQGGGSCCC 179
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 291,605,074
Number of Sequences: 636343
Number of extensions: 2925515
Number of successful extensions: 28949
Number of sequences better than 1.0e-020: 5
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 11880035741
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)