TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5735869.2.1
(539 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA709186.1|CA709186 wdk2c.pk012.e3 wdk2c Triticum aestiv... 77 3e-021
>gb|CA709186.1|CA709186 wdk2c.pk012.e3 wdk2c Triticum aestivum cDNA clone wdk2c.pk012.e3 5'
end, mRNA sequence
Length = 502
Score = 77.1 bits (162), Expect(2) = 3e-021
Identities = 28/40 (70%), Positives = 31/40 (77%)
Frame = -1 / +3
Query: 500 GKGYXXXWDXERMATINDFIWTDSEISKMVRCFDSDGDDK 381
GKGY WD E+MA INDFIWTD E+SKM+ CFDSD D K
Sbjct: 141 GKGYIAPWDLEKMANINDFIWTDFELSKMIHCFDSDKDGK 260
Score = 44.6 bits (91), Expect(2) = 3e-021
Identities = 18/25 (72%), Positives = 19/25 (76%)
Frame = -1 / +2
Query: 386 DKPRGFPQHCISMQHVAGA*EVRMT 312
DKPRG P H +SMQHVAGA V MT
Sbjct: 260 DKPRGIPHHRVSMQHVAGARGVMMT 334
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 428,549,571
Number of Sequences: 636343
Number of extensions: 5636288
Number of successful extensions: 89284
Number of sequences better than 1.0e-020: 2
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 11344094279
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)