TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5351892.2.1
(466 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BT009236.1| Triticum aestivum clone wle1n.pk0109.e10:fis... 158 3e-038
gb|BJ320027.1|BJ320027 BJ320027 Y. Ogihara unpublished cDNA... 157 8e-038
>gb|BT009236.1| Triticum aestivum clone wle1n.pk0109.e10:fis, full insert mRNA
sequence
Length = 1262
Score = 158 bits (340), Expect = 3e-038
Identities = 58/73 (79%), Positives = 66/73 (90%)
Frame = +3 / +1
Query: 9 AGLKPWIMLKSENHVTPDPPCVRAMSIEPCFHQAPFYDCKARKDTDLGKVVPYVRHCEDV 188
AGL PWIM + ENHV P+PPC RAMSIEPCFHQAP++DCKA++D DLGKVVPYVRHCEDV
Sbjct: 790 AGLTPWIMYRPENHVVPEPPCGRAMSIEPCFHQAPYFDCKAKRDADLGKVVPYVRHCEDV 969
Query: 189 SWGLKIVDQTHL* 227
SWGLKIV++T L*
Sbjct: 970 SWGLKIVNETRL* 1008
Score = 126 bits (270), Expect(2) = 7e-029
Identities = 56/73 (76%), Positives = 57/73 (78%)
Frame = -1 / -1
Query: 226 YK*V*STILSPQLTSSQCLT*GTTLPKSVSFLALQS*KGA*WKHGSMDMARTHGGSGVTW 47
Y V TIL PQLTSSQCLT GTTLP+S S LALQS GA WKHGSMDMAR HGGSG TW
Sbjct: 1007 YNRVSFTILRPQLTSSQCLTYGTTLPRSASLLALQSK*GAWWKHGSMDMARPHGGSGTTW 828
Query: 46 FSDLSMIHGFRPA 8
FS L MIHG RPA
Sbjct: 827 FSGLYMIHGVRPA 789
Score = 112 bits (239), Expect = 3e-024
Identities = 49/72 (68%), Positives = 53/72 (73%)
Frame = +2 / +3
Query: 11 RPEAVDHA*VGEPRDARPTVRPRHVHRAVLPSSSLLRLQGEEGHRFGQGSALREALRGRE 190
RP AVDH EPR A VRP HVHRAVLP LLRLQG+EG R GQG A+REALRGR+
Sbjct: 792 RPHAVDHVQAREPRRAGAAVRPCHVHRAVLPPGPLLRLQGQEGRRPGQGGAVREALRGRQ 971
Query: 191 LGAQDCRSNSFV 226
LG QDC+ NS V
Sbjct: 972 LGPQDCK*NSVV 1007
Score = 103 bits (219), Expect = 2e-021
Identities = 48/73 (65%), Positives = 53/73 (72%)
Frame = -3 / -3
Query: 227 LQMSLIYNLEPPAHVLAVPHVRHYLAQIGVLPRLAVVEGSLMEARLDGHGADARWVWRHV 48
LQ S IYNLE PA VLAVPHVRH+LAQ+GV LAV G L+EARLDGHG AR + V
Sbjct: 1008 LQPSFIYNLEAPADVLAVPHVRHHLAQVGVPLGLAVEVGGLVEARLDGHGTAARRLRHDV 829
Query: 47 VLRLKHDPRLQAG 9
VL HDPR +AG
Sbjct: 828 VLGPVHDPRREAG 790
Score = 22.6 bits (43), Expect(2) = 7e-029
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = -2 / -1
Query: 432 FLFLLTSEDEQINGSVYQLSTL 367
F F IN SVYQLST+
Sbjct: 1208 FFFFXVIIVFSINESVYQLSTI 1143
>gb|BJ320027.1|BJ320027 BJ320027 Y. Ogihara unpublished cDNA library, Wh_yf Triticum
aestivum cDNA clone whyf13c22 3', mRNA sequence
Length = 699
Score = 157 bits (337), Expect = 8e-038
Identities = 57/73 (78%), Positives = 66/73 (90%)
Frame = +3 / -2
Query: 9 AGLKPWIMLKSENHVTPDPPCVRAMSIEPCFHQAPFYDCKARKDTDLGKVVPYVRHCEDV 188
AGL PWIM + ENHV P+PPC RAMSIEPCFHQAP++DC+A++D DLGKVVPYVRHCEDV
Sbjct: 392 AGLTPWIMYRPENHVVPEPPCGRAMSIEPCFHQAPYFDCRAKRDADLGKVVPYVRHCEDV 213
Query: 189 SWGLKIVDQTHL* 227
SWGLKIV++T L*
Sbjct: 212 SWGLKIVNETRL* 174
Score = 127 bits (271), Expect = 1e-028
Identities = 56/73 (76%), Positives = 57/73 (78%)
Frame = -1 / +1
Query: 226 YK*V*STILSPQLTSSQCLT*GTTLPKSVSFLALQS*KGA*WKHGSMDMARTHGGSGVTW 47
Y V TILSPQLTSSQCLT GTTLP+S S ALQS GA WKHGSMDMAR HGGSG TW
Sbjct: 175 YNRVSFTILSPQLTSSQCLTYGTTLPRSASLFALQSK*GAWWKHGSMDMARPHGGSGTTW 354
Query: 46 FSDLSMIHGFRPA 8
FS L MIHG RPA
Sbjct: 355 FSGLYMIHGVRPA 393
Score = 112 bits (239), Expect = 3e-024
Identities = 50/72 (69%), Positives = 53/72 (73%)
Frame = +2 / -1
Query: 11 RPEAVDHA*VGEPRDARPTVRPRHVHRAVLPSSSLLRLQGEEGHRFGQGSALREALRGRE 190
RP AVDH EPR A VRP HVHRAVLP LLRLQGEEG R GQG A+REALR R+
Sbjct: 390 RPHAVDHVQAREPRRAGAAVRPCHVHRAVLPPGPLLRLQGEEGRRPGQGGAVREALRRRQ 211
Query: 191 LGAQDCRSNSFV 226
LGAQDC+ NS V
Sbjct: 210 LGAQDCK*NSVV 175
Score = 105 bits (223), Expect = 4e-022
Identities = 48/73 (65%), Positives = 53/73 (72%)
Frame = -3 / +3
Query: 227 LQMSLIYNLEPPAHVLAVPHVRHYLAQIGVLPRLAVVEGSLMEARLDGHGADARWVWRHV 48
LQ S IYNLEPPA V AVPHVRH+LAQ+GV R AV G L+EARLDGHG AR + V
Sbjct: 174 LQPSFIYNLEPPADVFAVPHVRHHLAQVGVPLRPAVEVGGLVEARLDGHGTAARRLRHDV 353
Query: 47 VLRLKHDPRLQAG 9
VL HDPR +AG
Sbjct: 354 VLGPVHDPRREAG 392
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 411,755,609
Number of Sequences: 636343
Number of extensions: 5347295
Number of successful extensions: 147318
Number of sequences better than 1.0e-020: 4
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 9111004854
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)