TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4790360.2.1
(667 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CV066458.1|CV066458 WNEL4b5 Wheat EST endosperm library ... 238 4e-062
gb|BF473884.1|BF473884 WHE0932_D09_H18ZS Wheat 5-15 DAP spi... 228 5e-059
>gb|CV066458.1|CV066458 WNEL4b5 Wheat EST endosperm library Triticum aestivum cDNA clone
WNEL4b5 5' similar to Oryza sativa, mRNA sequence
Length = 546
Score = 238 bits (515), Expect = 4e-062
Identities = 92/115 (80%), Positives = 98/115 (85%)
Frame = +2 / +2
Query: 2 HEPQSFLLGSQYFLHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDVTIGSWMLAMNVNH 181
+EPQSFLLGS+YFLHAYGPIY RNNSFRMF+NEDVTIGSWMLAMNVNH
Sbjct: 56 YEPQSFLLGSEYFLHAYGPIYALSADVVASLVALRNNSFRMFNNEDVTIGSWMLAMNVNH 235
Query: 182 ENTHALCEPDCTESSIAVWDIPKCSGLCHPEVKMLELHERKECTGGPTVAAEVSE 346
ENTHALCEP+CT SSIAVWDIPKCSGLCHPEVKMLELH+RKECTGGPT AAE +
Sbjct: 236 ENTHALCEPECTASSIAVWDIPKCSGLCHPEVKMLELHQRKECTGGPTEAAETDD 400
Score = 200 bits (432), Expect = 1e-050
Identities = 87/115 (75%), Positives = 93/115 (80%)
Frame = -2 / -1
Query: 345 SDTSAATVGPPVHXXXXXXXXXXXSGWHRPEHFGMSQTAIEDSVQSGSQSACVFSWLTFI 166
S SAA+VGPPVH SGWH PEHFG+SQTAIED+V SGS ACVFSWLTFI
Sbjct: 399 SSVSAASVGPPVHSFRW*SSSIFTSGWHSPEHFGISQTAIEDAVHSGSHRACVFSWLTFI 220
Query: 165 ASIHDPIVTSSLLNIRKLLFRNATKLATTSADKA*IGPYA*RKYCDPSKKDWGSW 1
ASI DPIVTSSLLNIRKLLF AT+LATTSADKA*IGPYA*RKY DPS+KD GS+
Sbjct: 219 ASIQDPIVTSSLLNIRKLLFLRATRLATTSADKA*IGPYA*RKYSDPSRKDCGSY 55
Score = 162 bits (349), Expect = 3e-039
Identities = 69/108 (63%), Positives = 79/108 (73%)
Frame = +1 / +1
Query: 7 APILFTRITILPSCIWANLCFIS*RGG*LGCIAKQQFPYVQQRGRYNWIVDACYERQP*E 186
A +L T I ILPSCIWA LCFIS* GG GC KQQF VQQ G Y WI+DACYERQP E
Sbjct: 61 ATVLPTWIRILPSCIWAYLCFIS*CGGKPGCSEKQQFSNVQQ*GCYYWILDACYERQPRE 240
Query: 187 HTCTL*T*LHGVFNCCLGHPKMLRSMPPRGKNARAP*KKRMHRWSNCG 330
H C++ T +H V NCCLG+ KMLR+MPP GK+A A +R+H WSN G
Sbjct: 241 HACSVRTRVHSVLNCCLGYSKMLRAMPPGGKDAGASPAERVHGWSNGG 384
Score = 152 bits (326), Expect = 4e-036
Identities = 68/107 (63%), Positives = 84/107 (78%)
Frame = -1 / -3
Query: 322 WTTCAFFSFMEL*HFYLWVA*T*AFWDVPNSN*RLRAVRFTKCMCVLMVDVHSKHPRSNC 143
WTT A F ++L H YL VA* *AFW++P+SN* A+ F + M VL+VDVHSKHP SN
Sbjct: 376 WTTRALFPLVKLQHLYLRVA*P*AFWNIPDSN*GRCALGFAQSMRVLVVDVHSKHPGSNS 197
Query: 142 NVLVAEHTETVVSQCNQASHHVS**SINWPICMKEVL*SE*KGLGLM 2
N+L+ EH++TVVSQ NQA HH+S**SIN PICMKEV *S+*+GL L+
Sbjct: 196 NILIVEHSKTVVSQSNQACHHIS**SINRPICMKEVF*SK*EGLWLV 56
Score = 148 bits (318), Expect = 5e-035
Identities = 68/107 (63%), Positives = 76/107 (71%)
Frame = -3 / -2
Query: 335 QLPQLDHLCILFFHGALAFLPLGGIDLSILGCPKQQLKTPCSQVHKVHVCSHG*RS*QAS 156
Q P LDH C L A A LP GGI LSIL P+QQL+T C++V H CS G*RS*QAS
Sbjct: 389 QRPPLDHPCTLSAGEAPASLPPGGIALSILEYPRQQLRTLCTRVRTEHACSRG*RS*QAS 210
Query: 155 TIQL*RPRC*TYGNCCFAMQPS*PPRQLIKHKLAHMHEGSIVIRVKR 15
IQ * P C*T+ NCCF+ QP PP QLIKHK AHMHEGSI+I+V R
Sbjct: 209 RIQ**HPHC*TFENCCFSEQPGLPPHQLIKHK*AHMHEGSILIQVGR 69
Score = 140 bits (301), Expect = 1e-032
Identities = 66/106 (62%), Positives = 73/106 (68%)
Frame = +3 / +3
Query: 6 SPNPFYSDHNTSFMHMGQFMLYQLTWWLAWLHCETTVSVCSATRTLQLDRGCLL*TSTMR 185
S +P Y D NTSFMHMG FMLYQL WW AWL ETTV CS R L LD GCLL*TST R
Sbjct: 60 SHSPSYLDQNTSFMHMGLFMLYQLMWWQAWLL*ETTVFECSTMRMLLLDPGCLL*TSTTR 239
Query: 186 THMHFVNLTARSLQLLFGTSQNAQVYATQR*KC*SSMKEKNAQVVQ 323
T M N +A+ QLL G QNAQ YAT+R*+C S K+A+VVQ
Sbjct: 240 TRMLCANPSAQRPQLLSGIFQNAQGYATRR*RCWSFTSGKSARVVQ 377
>gb|BF473884.1|BF473884 WHE0932_D09_H18ZS Wheat 5-15 DAP spike cDNA library Triticum
aestivum cDNA clone WHE0932_D09_H18, mRNA sequence
Length = 651
Score = 228 bits (492), Expect = 5e-059
Identities = 88/108 (81%), Positives = 93/108 (86%)
Frame = +2 / +3
Query: 2 HEPQSFLLGSQYFLHAYGPIYXXXXXXXXXXXXXRNNSFRMFSNEDVTIGSWMLAMNVNH 181
+EPQSFLLGS+YFLHAYGPIY RNNSFRMF+NEDVTIGSWMLAMNVNH
Sbjct: 99 YEPQSFLLGSEYFLHAYGPIYALSADVVASLVAVRNNSFRMFNNEDVTIGSWMLAMNVNH 278
Query: 182 ENTHALCEPDCTESSIAVWDIPKCSGLCHPEVKMLELHERKECTGGPT 325
ENTHAL EP+CT SSIAVWDIPKCSGLCHPEVKMLELH+RKECTGGPT
Sbjct: 279 ENTHALTEPECTASSIAVWDIPKCSGLCHPEVKMLELHQRKECTGGPT 422
Score = 196 bits (422), Expect = 2e-049
Identities = 85/110 (77%), Positives = 90/110 (81%)
Frame = -2 / -3
Query: 330 ATVGPPVHXXXXXXXXXXXSGWHRPEHFGMSQTAIEDSVQSGSQSACVFSWLTFIASIHD 151
++VGPPVH SGWH PEHFGMSQTAIED+V SGS ACVFSWLTFIASI D
Sbjct: 427 SSVGPPVHSFRW*SSSIFTSGWHSPEHFGMSQTAIEDAVHSGSVRACVFSWLTFIASIQD 248
Query: 150 PIVTSSLLNIRKLLFRNATKLATTSADKA*IGPYA*RKYCDPSKKDWGSW 1
PIVTSSLLNIRKLLF ATKLATTSADKA*IGPYA*RKY DPS+KD GS+
Sbjct: 247 PIVTSSLLNIRKLLFLTATKLATTSADKA*IGPYA*RKYSDPSRKDCGSY 98
Score = 167 bits (360), Expect = 9e-041
Identities = 73/112 (65%), Positives = 82/112 (73%)
Frame = +1 / +2
Query: 7 APILFTRITILPSCIWANLCFIS*RGG*LGCIAKQQFPYVQQRGRYNWIVDACYERQP*E 186
A +L T I ILPSCIWA LCFIS* GG LGC KQQFP VQQRG Y WI+DA YERQP E
Sbjct: 104 ATVLPTWIRILPSCIWAYLCFIS*CGGKLGCCEKQQFPDVQQRGCYYWILDARYERQPRE 283
Query: 187 HTCTL*T*LHGVFNCCLGHPKMLRSMPPRGKNARAP*KKRMHRWSNCGS*GV 342
H + T +H V NCCLGH KMLR+MPP GK+ARA +R+H WSN G G+
Sbjct: 284 HARSHRTRVHSVLNCCLGHSKMLRAMPPGGKDARASPAERVHGWSNGGPGGM 439
Score = 155 bits (334), Expect = 3e-037
Identities = 71/108 (65%), Positives = 79/108 (73%)
Frame = -3 / -1
Query: 338 PQLPQLDHLCILFFHGALAFLPLGGIDLSILGCPKQQLKTPCSQVHKVHVCSHG*RS*QA 159
P P LDH C L ALA LP GGI LSIL CP+QQL+T C++V CS G*RS*+A
Sbjct: 435 PPGPPLDHPCTLSAGEALASLPPGGIALSILECPRQQLRTLCTRVR*ERACSRG*RS*RA 256
Query: 158 STIQL*RPRC*TYGNCCFAMQPS*PPRQLIKHKLAHMHEGSIVIRVKR 15
S IQ * PRC*T GNCCF+ QPS PP QLIKHK AHMHEGSI+I+V R
Sbjct: 255 SRIQ**HPRC*TSGNCCFSQQPSLPPHQLIKHK*AHMHEGSILIQVGR 112
Score = 149 bits (320), Expect = 3e-035
Identities = 69/107 (64%), Positives = 83/107 (77%)
Frame = -1 / -2
Query: 322 WTTCAFFSFMEL*HFYLWVA*T*AFWDVPNSN*RLRAVRFTKCMCVLMVDVHSKHPRSNC 143
WTT A F ++L*H YL VA* *AFW+VP+SN* A+ F + + VL+VDVHS+HP SN
Sbjct: 419 WTTRALFPLVKL*HLYLRVA*P*AFWNVPDSN*GRCALGFGESVRVLVVDVHSEHPGSNS 240
Query: 142 NVLVAEHTETVVSQCNQASHHVS**SINWPICMKEVL*SE*KGLGLM 2
N+LV EH ETVVS NQA HH+S**SIN PICMKEV *S+*+GL L+
Sbjct: 239 NILVVEHPETVVSHSNQACHHIS**SINRPICMKEVF*SK*EGLWLV 99
Score = 144 bits (309), Expect = 1e-033
Identities = 68/106 (64%), Positives = 75/106 (70%)
Frame = +3 / +1
Query: 6 SPNPFYSDHNTSFMHMGQFMLYQLTWWLAWLHCETTVSVCSATRTLQLDRGCLL*TSTMR 185
S +P Y D NTSFMHMG FMLYQL WW AWL ETTVS CS TR L LD GC L*TST R
Sbjct: 103 SHSPSYLDQNTSFMHMGLFMLYQLMWWQAWLL*ETTVSGCSTTRMLLLDPGCSL*TSTTR 282
Query: 186 THMHFVNLTARSLQLLFGTSQNAQVYATQR*KC*SSMKEKNAQVVQ 323
T N +A+ QLL GT QNAQ YAT+R*+C*S K+A+VVQ
Sbjct: 283 TRTLSPNPSAQRPQLLSGTFQNAQGYATRR*RC*SFTSGKSARVVQ 420
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 669,908,113
Number of Sequences: 636343
Number of extensions: 10379842
Number of successful extensions: 90743
Number of sequences better than 1.0e-020: 2
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 14899455312
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)