TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3923331.2.1
(496 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ167130.1|BQ167130 WHE0946_F09_K18ZY Wheat 5-15 DAP spi... 199 2e-050
gb|CD907374.1|CD907374 G468.106L19R010929 G468 Triticum aes... 199 2e-050
emb|AJ582182.1|TAE582182 Triticum aestivum mRNA for putativ... 199 2e-050
gb|CA502151.1|CA502151 WHE4043_D07_H13ZT Wheat meiotic anth... 185 3e-046
gb|CA602908.1|CA602908 wr1.pk0007.e11 wr1 Triticum aestivum... 182 2e-045
>gb|BQ167130.1|BQ167130 WHE0946_F09_K18ZY Wheat 5-15 DAP spike cDNA library Triticum
aestivum cDNA clone WHE0946_F09_K18, mRNA sequence
Length = 701
Score = 199 bits (429), Expect = 2e-050
Identities = 81/100 (81%), Positives = 92/100 (92%)
Frame = -3 / -3
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+ESIY+RS QLTLGALESAV GKFRSL HVPVWKDERP SIRGGD+LK+Y+IY
Sbjct: 474 RERGRFKTESIYLRSDQLTLGALESAVHGKFRSLKHVPVWKDERPSSIRGGDELKVYKIY 295
Query: 311 PVGLTQRQALYGFRFRDDSELEQYIKDHPCAKLEVIFV*L 192
P+GLT+RQALY F+F DD+E+ +YIK HPCAKLEVIFV*L
Sbjct: 294 PIGLTERQALYKFQFSDDAEVARYIKGHPCAKLEVIFV*L 175
Score = 130 bits (278), Expect = 1e-029
Identities = 62/102 (60%), Positives = 64/102 (62%)
Frame = +1 / +2
Query: 187 HSSYTKITSSFAQGWSLIYCXXXXXXLNLKPYKA*RCVRPTG*ILYNFRXXXXXXXXXXX 366
+ SYTKITSSFA GW LIY LN Y A VRP G*ILY F
Sbjct: 170 YCSYTKITSSFAHGWPLIYLATSASSLN*NLYNACLSVRPIG*ILYTFNSSPPRILDGLS 349
Query: 367 XFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDLNRPLSL 492
FHTGT LRDLNLP TADSKAP+VN DL *MLS LNRPLSL
Sbjct: 350 SFHTGTCLRDLNLPCTADSKAPKVN*SDLR*MLSVLNRPLSL 475
Score = 111 bits (236), Expect = 8e-024
Identities = 56/100 (56%), Positives = 66/100 (66%)
Frame = +3 / +1
Query: 192 *LHKNYFKLCTGMVFDILLEFRIIPKSKTVQSLTLR*TDWVNSV*FQVIPTSNARGPFIL 371
*L+KN+ KLCT M +I F II + K + L+ T WVN V Q+IP SN PFIL
Sbjct: 175 *LYKNHLKLCTWMALNISSNFSIITELKFI*RLSFCKTYWVNFVHLQLIPASNT*WPFIL 354
Query: 372 PYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSLS 491
PYRN++EG KFT H Q S S*L *S +NAL LKPPSLS
Sbjct: 355 PYRNMLEGSKFTMHGRLQSS*S*LI*S*INALCLKPPSLS 474
Score = 89.0 bits (188), Expect(2) = 2e-021
Identities = 40/61 (65%), Positives = 45/61 (73%)
Frame = -1 / -1
Query: 490 EREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGPSTTFLYGRMKGPRALEVGMT*NYTEFT 311
EREGGL QRAF *DQ S*L E W+ C VNL PS+ FLYGRMKG + E GM+* T+FT
Sbjct: 473 EREGGLRQRAFI*DQIS*L*ELWSLPCMVNLDPSSMFLYGRMKGHQVFEAGMS*RCTKFT 294
Query: 310 Q 308
Q
Sbjct: 293 Q 291
Score = 33.6 bits (67), Expect(2) = 2e-021
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = -2 / -2
Query: 243 IYQRPSLCKA*SNFCVTTM 187
IY PS+CKA* +FC+TT+
Sbjct: 226 IY*GPSMCKA*GDFCITTI 170
>gb|CD907374.1|CD907374 G468.106L19R010929 G468 Triticum aestivum cDNA clone G468106L19,
mRNA sequence
Length = 769
Score = 199 bits (429), Expect = 2e-050
Identities = 81/100 (81%), Positives = 92/100 (92%)
Frame = -3 / -3
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+ESIY+RS QLTLGALESAV GKFRSL HVPVWKDERP SIRGGD+LK+Y+IY
Sbjct: 482 RERGRFKTESIYLRSDQLTLGALESAVHGKFRSLKHVPVWKDERPSSIRGGDELKVYKIY 303
Query: 311 PVGLTQRQALYGFRFRDDSELEQYIKDHPCAKLEVIFV*L 192
P+GLT+RQALY F+F DD+E+ +YIK HPCAKLEVIFV*L
Sbjct: 302 PIGLTERQALYKFQFSDDAEVARYIKGHPCAKLEVIFV*L 183
Score = 130 bits (278), Expect = 1e-029
Identities = 62/102 (60%), Positives = 64/102 (62%)
Frame = +1 / +1
Query: 187 HSSYTKITSSFAQGWSLIYCXXXXXXLNLKPYKA*RCVRPTG*ILYNFRXXXXXXXXXXX 366
+ SYTKITSSFA GW LIY LN Y A VRP G*ILY F
Sbjct: 178 YCSYTKITSSFAHGWPLIYLATSASSLN*NLYNACLSVRPIG*ILYTFNSSPPRILDGLS 357
Query: 367 XFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDLNRPLSL 492
FHTGT LRDLNLP TADSKAP+VN DL *MLS LNRPLSL
Sbjct: 358 SFHTGTCLRDLNLPCTADSKAPKVN*SDLR*MLSVLNRPLSL 483
Score = 111 bits (236), Expect = 8e-024
Identities = 56/100 (56%), Positives = 66/100 (66%)
Frame = +3 / +3
Query: 192 *LHKNYFKLCTGMVFDILLEFRIIPKSKTVQSLTLR*TDWVNSV*FQVIPTSNARGPFIL 371
*L+KN+ KLCT M +I F II + K + L+ T WVN V Q+IP SN PFIL
Sbjct: 183 *LYKNHLKLCTWMALNISSNFSIITELKFI*RLSFCKTYWVNFVHLQLIPASNT*WPFIL 362
Query: 372 PYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSLS 491
PYRN++EG KFT H Q S S*L *S +NAL LKPPSLS
Sbjct: 363 PYRNMLEGSKFTMHGRLQSS*S*LI*S*INALCLKPPSLS 482
Score = 89.0 bits (188), Expect(2) = 2e-021
Identities = 40/61 (65%), Positives = 45/61 (73%)
Frame = -1 / -1
Query: 490 EREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGPSTTFLYGRMKGPRALEVGMT*NYTEFT 311
EREGGL QRAF *DQ S*L E W+ C VNL PS+ FLYGRMKG + E GM+* T+FT
Sbjct: 481 EREGGLRQRAFI*DQIS*L*ELWSLPCMVNLDPSSMFLYGRMKGHQVFEAGMS*RCTKFT 302
Query: 310 Q 308
Q
Sbjct: 301 Q 299
Score = 33.6 bits (67), Expect(2) = 2e-021
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = -2 / -2
Query: 243 IYQRPSLCKA*SNFCVTTM 187
IY PS+CKA* +FC+TT+
Sbjct: 234 IY*GPSMCKA*GDFCITTI 178
>emb|AJ582182.1|TAE582182 Triticum aestivum mRNA for putative glycoprotein
3-alpha-L-fucosyltransferase (core3ft gene)
Length = 1750
Score = 199 bits (429), Expect = 2e-050
Identities = 81/100 (81%), Positives = 92/100 (92%)
Frame = -3 / +2
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+ESIY+RS QLTLGALESAV GKFRSL HVPVWKDERP SIRGGD+LK+Y+IY
Sbjct: 1235 RERGRFKTESIYLRSDQLTLGALESAVHGKFRSLKHVPVWKDERPSSIRGGDELKVYKIY 1414
Query: 311 PVGLTQRQALYGFRFRDDSELEQYIKDHPCAKLEVIFV*L 192
P+GLT+RQALY F+F DD+E+ +YIK HPCAKLEVIFV*L
Sbjct: 1415 PIGLTERQALYKFQFSDDAEVARYIKGHPCAKLEVIFV*L 1534
Score = 130 bits (278), Expect = 1e-029
Identities = 62/102 (60%), Positives = 64/102 (62%)
Frame = +1 / -2
Query: 187 HSSYTKITSSFAQGWSLIYCXXXXXXLNLKPYKA*RCVRPTG*ILYNFRXXXXXXXXXXX 366
+ SYTKITSSFA GW LIY LN Y A VRP G*ILY F
Sbjct: 1539 YCSYTKITSSFAHGWPLIYLATSASSLN*NLYNACLSVRPIG*ILYTFNSSPPRILDGLS 1360
Query: 367 XFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDLNRPLSL 492
FHTGT LRDLNLP TADSKAP+VN DL *MLS LNRPLSL
Sbjct: 1359 SFHTGTCLRDLNLPCTADSKAPKVN*SDLR*MLSVLNRPLSL 1234
Score = 111 bits (236), Expect = 8e-024
Identities = 56/100 (56%), Positives = 66/100 (66%)
Frame = +3 / -1
Query: 192 *LHKNYFKLCTGMVFDILLEFRIIPKSKTVQSLTLR*TDWVNSV*FQVIPTSNARGPFIL 371
*L+KN+ KLCT M +I F II + K + L+ T WVN V Q+IP SN PFIL
Sbjct: 1534 *LYKNHLKLCTWMALNISSNFSIITELKFI*RLSFCKTYWVNFVHLQLIPASNT*WPFIL 1355
Query: 372 PYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSLS 491
PYRN++EG KFT H Q S S*L *S +NAL LKPPSLS
Sbjct: 1354 PYRNMLEGSKFTMHGRLQSS*S*LI*S*INALCLKPPSLS 1235
Score = 89.0 bits (188), Expect(2) = 2e-021
Identities = 40/61 (65%), Positives = 45/61 (73%)
Frame = -1 / +3
Query: 490 EREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGPSTTFLYGRMKGPRALEVGMT*NYTEFT 311
EREGGL QRAF *DQ S*L E W+ C VNL PS+ FLYGRMKG + E GM+* T+FT
Sbjct: 1236 EREGGLRQRAFI*DQIS*L*ELWSLPCMVNLDPSSMFLYGRMKGHQVFEAGMS*RCTKFT 1415
Query: 310 Q 308
Q
Sbjct: 1416 Q 1418
Score = 33.6 bits (67), Expect(2) = 2e-021
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = -2 / +1
Query: 243 IYQRPSLCKA*SNFCVTTM 187
IY PS+CKA* +FC+TT+
Sbjct: 1483 IY*GPSMCKA*GDFCITTI 1539
>gb|CA502151.1|CA502151 WHE4043_D07_H13ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE4043_D07_H13, mRNA sequence
Length = 534
Score = 185 bits (398), Expect = 3e-046
Identities = 76/96 (79%), Positives = 87/96 (90%)
Frame = -3 / +1
Query: 479 RFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGL 300
R K+ESIY+RS QLTLGALESAV GKFRSL HVPVWKDERP SIRGGD+LK+Y+IYP+GL
Sbjct: 31 RPKTESIYLRSDQLTLGALESAVHGKFRSLKHVPVWKDERPSSIRGGDELKVYKIYPIGL 210
Query: 299 TQRQALYGFRFRDDSELEQYIKDHPCAKLEVIFV*L 192
T+RQALY F+F DD+E+ +YIK HPCAKLEVIFV*L
Sbjct: 211 TERQALYKFQFSDDAEVARYIKGHPCAKLEVIFV*L 318
Score = 116 bits (249), Expect = 1e-025
Identities = 56/98 (57%), Positives = 58/98 (59%)
Frame = +1 / -2
Query: 187 HSSYTKITSSFAQGWSLIYCXXXXXXLNLKPYKA*RCVRPTG*ILYNFRXXXXXXXXXXX 366
+ SYTKITSSFA GW LIY LN Y A VRP G*ILY F
Sbjct: 323 YCSYTKITSSFAHGWPLIYLATSASSLN*NLYNACLSVRPIG*ILYTFNSSPPRILDGLS 144
Query: 367 XFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDLNR 480
FHTGT LRDLNLP TADSKAP+VN DL *MLS R
Sbjct: 143 SFHTGTCLRDLNLPCTADSKAPKVN*SDLR*MLSVFGR 30
>gb|CA602908.1|CA602908 wr1.pk0007.e11 wr1 Triticum aestivum cDNA clone wr1.pk0007.e11 5'
end, mRNA sequence
Length = 487
Score = 182 bits (392), Expect = 2e-045
Identities = 72/91 (79%), Positives = 83/91 (91%)
Frame = -3 / +1
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+ESIY+RS QLTLGALESAV GKFRSL HVPVWKDERP SIRGGD+LK+Y+IY
Sbjct: 82 RERGRFKTESIYLRSDQLTLGALESAVHGKFRSLKHVPVWKDERPSSIRGGDELKVYKIY 261
Query: 311 PVGLTQRQALYGFRFRDDSELEQYIKDHPCA 219
P+GLT+RQALY F+F DD+E+ +YIK HPCA
Sbjct: 262 PIGLTERQALYKFQFSDDAEVARYIKGHPCA 354
Score = 112 bits (239), Expect = 3e-024
Identities = 54/92 (58%), Positives = 55/92 (59%)
Frame = +1 / -3
Query: 217 FAQGWSLIYCXXXXXXLNLKPYKA*RCVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRD 396
FA GW LIY LN Y A VRP G*ILY F FHTGT LRD
Sbjct: 356 FAHGWPLIYLATSASSLN*NLYNACLSVRPIG*ILYTFNSSPPRILDGLSSFHTGTCLRD 177
Query: 397 LNLPSTADSKAPRVNWPDLM*MLSDLNRPLSL 492
LNLP TADSKAP+VN DL *MLS LNRPLSL
Sbjct: 176 LNLPCTADSKAPKVN*SDLR*MLSVLNRPLSL 81
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 458,046,882
Number of Sequences: 636343
Number of extensions: 6062428
Number of successful extensions: 91094
Number of sequences better than 1.0e-020: 6
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 10004240624
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)