TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3923171.2.1
(570 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA486940.1|CA486940 WHE4337_G03_M05ZS Wheat meiotic anth... 67 3e-025
>gb|CA486940.1|CA486940 WHE4337_G03_M05ZS Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE4337_G03_M05, mRNA sequence
Length = 415
Score = 66.6 bits (139), Expect(3) = 3e-025
Identities = 27/36 (75%), Positives = 28/36 (77%)
Frame = -1 / -3
Query: 423 ML*LDKVTA*LISWPQPPSAAWRVAPPRQPPWRYLL 316
ML LDKVT *LISWPQPP AAWR AP R PPW+ L
Sbjct: 182 MLELDKVTV*LISWPQPP*AAWRAAPRRPPPWQRYL 75
Score = 58.8 bits (122), Expect(3) = 1e-020
Identities = 30/48 (62%), Positives = 33/48 (68%)
Frame = +3 / +2
Query: 306 YYRPAGSAKEVDAAELRAKLLTEAAAN*LIKQSLCRATA*VGSLGCPP 449
Y +AKEVDAAELRAKLLTEAAAN*LI + CRA A + L P
Sbjct: 65 YRPAGSAAKEVDAAELRAKLLTEAAAN*LITRLPCRARACMSWLSTTP 208
Score = 46.4 bits (95), Expect(3) = 3e-025
Identities = 20/27 (74%), Positives = 20/27 (74%)
Frame = -2 / -1
Query: 326 ATCWAIVAGDRVRHPRLLLSCRSGSRV 246
ATCWAIVAG RV HPRLLL RS V
Sbjct: 82 ATCWAIVAGHRVWHPRLLLVNRSSGAV 2
Score = 46.0 bits (94), Expect(3) = 1e-020
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +3 / +2
Query: 282 WVPDPVTGYYRPAGSA 329
WVPDPVTGYYRPAGSA
Sbjct: 38 WVPDPVTGYYRPAGSA 85
Score = 39.6 bits (80), Expect(3) = 3e-025
Identities = 18/33 (54%), Positives = 22/33 (66%)
Frame = -2 / -1
Query: 506 ALGQRARGSTSANIKAGEARWTAK*ANSCCSST 408
ALGQ A STSA++KA EARW+ CSS+
Sbjct: 268 ALGQGAPRSTSADLKAREARWSGGQPTHACSSS 170
Score = 32.2 bits (64), Expect(3) = 1e-020
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +1 / +3
Query: 442 VHLASPALIFALVDPRARWPRA 507
+HLAS AL ALVD A WPRA
Sbjct: 204 LHLASLALRSALVDRGAPWPRA 269
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 499,705,160
Number of Sequences: 636343
Number of extensions: 8592475
Number of successful extensions: 217650
Number of sequences better than 1.0e-020: 1
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 12237330049
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)