TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3754578.2.1
(725 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ265891.1|BJ265891 BJ265891 Y. Ogihara unpublished cDNA... 162 6e-040
gb|BE493730.1|BE493730 WHE0574_F12_L24ZE Triticum monococcu... 160 3e-039
gb|BJ260192.1|BJ260192 BJ260192 Y. Ogihara unpublished cDNA... 153 2e-036
gb|CK209599.1|CK209599 FGAS021371 Triticum aestivum FGAS: L... 149 4e-035
gb|BE498794.1|BE498794 WHE0966_G09_N18ZS Wheat pre-anthesis... 119 3e-030
gb|CA704545.1|CA704545 wdk1c.pk011.g17 wdk1c Triticum aesti... 129 4e-030
gb|CD918504.1|CD918504 G608.109L01F010907 G608 Triticum aes... 119 2e-029
gb|CD454064.1|CD454064 WHE0966_G09_N18ZT CS wheat pre-anthe... 119 3e-026
gb|CA706096.1|CA706096 wdk1c.pk022.h16 wdk1c Triticum aesti... 83 5e-026
gb|CA593233.1|CA593233 wpa1c.pk003.d20 wpa1c Triticum aesti... 103 6e-025
gb|CD872897.1|CD872897 AZO2.121N15F010209 AZO2 Triticum aes... 88 9e-021
gb|BJ297738.1|BJ297738 BJ297738 Y. Ogihara unpublished cDNA... 88 9e-021
gb|DN828796.1|DN828796 KUCD01_03_D02_T3 WSWR cDNA library T... 88 9e-021
gb|BJ247659.1|BJ247659 BJ247659 Y. Ogihara unpublished cDNA... 88 9e-021
gb|BJ253760.1|BJ253760 BJ253760 Y. Ogihara unpublished cDNA... 88 9e-021
>gb|BJ265891.1|BJ265891 BJ265891 Y. Ogihara unpublished cDNA library, Wh_h Triticum
aestivum cDNA clone whh23o06 3', mRNA sequence
Length = 607
Score = 162 bits (348), Expect(2) = 6e-040
Identities = 60/65 (92%), Positives = 62/65 (95%)
Frame = -3 / -2
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASC 538
EDVSMGLWVEKFN T PV+YSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRG+ SC
Sbjct: 528 EDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGRPSC 349
Query: 537 CNYR* 523
CNYR*
Sbjct: 348 CNYR* 334
Score = 127 bits (271), Expect(2) = 3e-029
Identities = 56/67 (83%), Positives = 59/67 (88%)
Frame = +2 / +2
Query: 524 YL*LQQDA*PRTNLSHKHSICLGD**WAV**FSRQPYWQNFQLWEYWTGFVELNFSTHRP 703
YL*LQQD PRTNLSHKHSICLGD** AV**FSRQPYWQNFQL EY TG V LNFSTH P
Sbjct: 335 YL*LQQDGRPRTNLSHKHSICLGD**CAV**FSRQPYWQNFQL*EYLTGMV*LNFSTHSP 514
Query: 704 MLTSSLV 724
+LTSS++
Sbjct: 515 ILTSSIL 535
Score = 23.1 bits (44), Expect(2) = 6e-040
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -2 / -2
Query: 457 GWLVFSFST 431
GWLVFSF T
Sbjct: 273 GWLVFSFLT 247
Score = 22.1 bits (42), Expect(2) = 3e-029
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = +3 / +2
Query: 432 VEKENTSQP 458
V+KENTSQP
Sbjct: 248 VKKENTSQP 274
>gb|BE493730.1|BE493730 WHE0574_F12_L24ZE Triticum monococcum vegetative apex cDNA library
Triticum monococcum cDNA clone WHE0574_F12_L24, mRNA
sequence
Length = 655
Score = 160 bits (343), Expect(2) = 3e-039
Identities = 59/65 (90%), Positives = 61/65 (93%)
Frame = -3 / +1
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASC 538
EDVSMGLWVEKFN T PV+YSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLV G+ SC
Sbjct: 106 EDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVHGRPSC 285
Query: 537 CNYR* 523
CNYR*
Sbjct: 286 CNYR* 300
Score = 122 bits (262), Expect(2) = 6e-028
Identities = 55/67 (82%), Positives = 58/67 (86%)
Frame = +2 / -3
Query: 524 YL*LQQDA*PRTNLSHKHSICLGD**WAV**FSRQPYWQNFQLWEYWTGFVELNFSTHRP 703
YL*LQQD P TNLSHKHSICLGD** AV**FSRQPYWQNFQL EY TG V LNFSTH P
Sbjct: 299 YL*LQQDGRP*TNLSHKHSICLGD**CAV**FSRQPYWQNFQL*EYLTGMV*LNFSTHSP 120
Query: 704 MLTSSLV 724
+LTSS++
Sbjct: 119 ILTSSIL 99
Score = 23.1 bits (44), Expect(2) = 3e-039
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -2 / +1
Query: 457 GWLVFSFST 431
GWLVFSF T
Sbjct: 361 GWLVFSFLT 387
Score = 22.1 bits (42), Expect(2) = 6e-028
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = +3 / -3
Query: 432 VEKENTSQP 458
V+KENTSQP
Sbjct: 386 VKKENTSQP 360
>gb|BJ260192.1|BJ260192 BJ260192 Y. Ogihara unpublished cDNA library, Wh_h Triticum
aestivum cDNA clone whh23o06 5', mRNA sequence
Length = 588
Score = 153 bits (329), Expect = 2e-036
Identities = 56/61 (91%), Positives = 58/61 (95%)
Frame = -3 / +2
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASC 538
EDVSMGLWVEKFN T PV+YSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRG+ SC
Sbjct: 404 EDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGRPSC 583
Query: 537 C 535
C
Sbjct: 584 C 586
Score = 121 bits (259), Expect = 8e-027
Identities = 53/64 (82%), Positives = 56/64 (87%)
Frame = +2 / -1
Query: 533 LQQDA*PRTNLSHKHSICLGD**WAV**FSRQPYWQNFQLWEYWTGFVELNFSTHRPMLT 712
LQQD PRTNLSHKHSICLGD** AV**FSRQPYWQNFQL EY TG V LNFSTH P+LT
Sbjct: 588 LQQDGRPRTNLSHKHSICLGD**CAV**FSRQPYWQNFQL*EYLTGMV*LNFSTHSPILT 409
Query: 713 SSLV 724
SS++
Sbjct: 408 SSIL 397
>gb|CK209599.1|CK209599 FGAS021371 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1130
Score = 149 bits (319), Expect = 4e-035
Identities = 56/65 (86%), Positives = 60/65 (92%)
Frame = -3 / +3
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASC 538
EDVSMGLWVEKFN T PV+YSHSWKFCQYGCLENYYTAHYQSPR+MLCLWDKLVRG+ S
Sbjct: 612 EDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLENYYTAHYQSPRRMLCLWDKLVRGRPSL 791
Query: 537 CNYR* 523
+YR*
Sbjct: 792 LHYR* 806
Score = 115 bits (245), Expect = 7e-025
Identities = 52/67 (77%), Positives = 56/67 (83%)
Frame = +2 / -2
Query: 524 YL*LQQDA*PRTNLSHKHSICLGD**WAV**FSRQPYWQNFQLWEYWTGFVELNFSTHRP 703
YL* +D PRTNLSHKHSI LGD** AV**FSRQPYWQNFQL EY TG V LNFSTH P
Sbjct: 805 YL*CSRDGRPRTNLSHKHSIRLGD**CAV**FSRQPYWQNFQL*EYLTGMV*LNFSTHSP 626
Query: 704 MLTSSLV 724
+LTSS++
Sbjct: 625 ILTSSIL 605
>gb|BE498794.1|BE498794 WHE0966_G09_N18ZS Wheat pre-anthesis spike cDNA library Triticum
aestivum cDNA clone WHE0966_G09_N18, mRNA sequence
Length = 462
Score = 119 bits (255), Expect(2) = 3e-030
Identities = 42/51 (82%), Positives = 46/51 (90%)
Frame = -3 / +2
Query: 675 TNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR* 523
T PVQY HSWKFCQYGC++NY+TAHYQSPRQMLCLWDKL G+A CCNYR*
Sbjct: 95 TAPVQYIHSWKFCQYGCVDNYFTAHYQSPRQMLCLWDKLSLGRAQCCNYR* 247
Score = 33.1 bits (66), Expect(2) = 3e-030
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -3 / +2
Query: 717 EDVSMGLWVEKFNST 673
EDVSMG+WVE +N+T
Sbjct: 44 EDVSMGMWVEDYNTT 88
>gb|CA704545.1|CA704545 wdk1c.pk011.g17 wdk1c Triticum aestivum cDNA clone wdk1c.pk011.g17
5' end, mRNA sequence
Length = 459
Score = 129 bits (276), Expect(2) = 4e-030
Identities = 46/49 (93%), Positives = 48/49 (97%)
Frame = -3 / +3
Query: 669 PVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR* 523
PV+YSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRG+ SCCNYR*
Sbjct: 12 PVRYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGRPSCCNYR* 158
Score = 99.6 bits (211), Expect(2) = 5e-021
Identities = 44/49 (89%), Positives = 44/49 (89%)
Frame = +2 / -3
Query: 524 YL*LQQDA*PRTNLSHKHSICLGD**WAV**FSRQPYWQNFQLWEYWTG 670
YL*LQQD PRTNLSHKHSICLGD** AV**FSRQPYWQNFQL EY TG
Sbjct: 157 YL*LQQDGRPRTNLSHKHSICLGD**CAV**FSRQPYWQNFQL*EYLTG 11
Score = 23.1 bits (44), Expect(2) = 4e-030
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -2 / +3
Query: 457 GWLVFSFST 431
GWLVFSF T
Sbjct: 219 GWLVFSFLT 245
Score = 22.1 bits (42), Expect(2) = 5e-021
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = +3 / -3
Query: 432 VEKENTSQP 458
V+KENTSQP
Sbjct: 244 VKKENTSQP 218
>gb|CD918504.1|CD918504 G608.109L01F010907 G608 Triticum aestivum cDNA clone G608109L01,
mRNA sequence
Length = 710
Score = 119 bits (255), Expect(2) = 2e-029
Identities = 42/51 (82%), Positives = 46/51 (90%)
Frame = -3 / +2
Query: 675 TNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR* 523
T PVQY HSWKFCQYGC++NY+TAHYQSPRQMLCLWDKL G+A CCNYR*
Sbjct: 53 TAPVQYIHSWKFCQYGCVDNYFTAHYQSPRQMLCLWDKLSLGRAQCCNYR* 205
Score = 29.9 bits (59), Expect(2) = 2e-029
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = -3 / +2
Query: 717 EDVSMGLWVEKFNST 673
ED SMG+WVE +N+T
Sbjct: 2 EDGSMGMWVEDYNTT 46
>gb|CD454064.1|CD454064 WHE0966_G09_N18ZT CS wheat pre-anthesis spike cDNA library Triticum
aestivum cDNA clone WHE0966_G09_N18, mRNA sequence
Length = 726
Score = 119 bits (255), Expect = 3e-026
Identities = 42/51 (82%), Positives = 46/51 (90%)
Frame = -3 / -1
Query: 675 TNPVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR* 523
T PVQY HSWKFCQYGC++NY+TAHYQSPRQMLCLWDKL G+A CCNYR*
Sbjct: 717 TAPVQYIHSWKFCQYGCVDNYFTAHYQSPRQMLCLWDKLSLGRAQCCNYR* 565
>gb|CA706096.1|CA706096 wdk1c.pk022.h16 wdk1c Triticum aestivum cDNA clone wdk1c.pk022.h16
5' end, mRNA sequence
Length = 431
Score = 82.6 bits (174), Expect(2) = 5e-026
Identities = 30/33 (90%), Positives = 31/33 (93%)
Frame = -3 / +2
Query: 717 EDVSMGLWVEKFNSTNPVQYSHSWKFCQYGCLE 619
EDVSMGLWVEKFN T PV+YSHSWKFCQYGCLE
Sbjct: 242 EDVSMGLWVEKFNYTMPVRYSHSWKFCQYGCLE 340
Score = 56.1 bits (116), Expect(2) = 5e-026
Identities = 19/21 (90%), Positives = 21/21 (100%)
Frame = -3 / +3
Query: 621 ENYYTAHYQSPRQMLCLWDKL 559
+NYYTAHYQSPRQMLCLWDK+
Sbjct: 339 KNYYTAHYQSPRQMLCLWDKV 401
>gb|CA593233.1|CA593233 wpa1c.pk003.d20 wpa1c Triticum aestivum cDNA clone wpa1c.pk003.d20
5' end, mRNA sequence
Length = 575
Score = 103 bits (219), Expect(2) = 6e-025
Identities = 36/50 (72%), Positives = 43/50 (86%)
Frame = -3 / +3
Query: 669 PVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR*Q 520
PV+Y HS KFCQ+GC+++YYTAHYQSPRQMLCLWDKL G+ CCN R*+
Sbjct: 183 PVEYVHSVKFCQFGCIDDYYTAHYQSPRQMLCLWDKLQAGKPRCCNMR*R 332
Score = 31.8 bits (63), Expect(2) = 6e-025
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = -3 / +2
Query: 717 EDVSMGLWVEKFNST 673
EDVSMG+WV +FN T
Sbjct: 134 EDVSMGMWVGRFNHT 178
>gb|CD872897.1|CD872897 AZO2.121N15F010209 AZO2 Triticum aestivum cDNA clone AZO2121N15,
mRNA sequence
Length = 706
Score = 87.7 bits (185), Expect(2) = 9e-021
Identities = 32/50 (64%), Positives = 36/50 (72%)
Frame = -3 / +2
Query: 669 PVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR*Q 520
PV+Y H KF Q GC + YYTAHYQSP+ M+CLW KL G A CCN R*Q
Sbjct: 299 PVEYLHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR*Q 448
Score = 33.1 bits (66), Expect(2) = 9e-021
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -3 / +2
Query: 717 EDVSMGLWVEKFNST 673
EDVSMG+WV+KF+ T
Sbjct: 248 EDVSMGMWVQKFSKT 292
>gb|BJ297738.1|BJ297738 BJ297738 Y. Ogihara unpublished cDNA library, Wh_SL Triticum
aestivum cDNA clone whsl21n21 3', mRNA sequence
Length = 646
Score = 87.7 bits (185), Expect(2) = 9e-021
Identities = 32/50 (64%), Positives = 36/50 (72%)
Frame = -3 / -2
Query: 669 PVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR*Q 520
PV+Y H KF Q GC + YYTAHYQSP+ M+CLW KL G A CCN R*Q
Sbjct: 375 PVEYLHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR*Q 226
Score = 33.1 bits (66), Expect(2) = 9e-021
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -3 / -2
Query: 717 EDVSMGLWVEKFNST 673
EDVSMG+WV+KF+ T
Sbjct: 426 EDVSMGMWVQKFSKT 382
>gb|DN828796.1|DN828796 KUCD01_03_D02_T3 WSWR cDNA library Triticum aestivum cDNA, mRNA
sequence
Length = 597
Score = 87.7 bits (185), Expect(2) = 9e-021
Identities = 32/50 (64%), Positives = 36/50 (72%)
Frame = -3 / +3
Query: 669 PVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR*Q 520
PV+Y H KF Q GC + YYTAHYQSP+ M+CLW KL G A CCN R*Q
Sbjct: 117 PVEYLHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR*Q 266
Score = 33.1 bits (66), Expect(2) = 9e-021
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -3 / +3
Query: 717 EDVSMGLWVEKFNST 673
EDVSMG+WV+KF+ T
Sbjct: 66 EDVSMGMWVQKFSKT 110
>gb|BJ247659.1|BJ247659 BJ247659 Y. Ogihara unpublished cDNA library, Wh_f Triticum
aestivum cDNA clone whf3c24 5', mRNA sequence
Length = 589
Score = 87.7 bits (185), Expect(2) = 9e-021
Identities = 32/50 (64%), Positives = 36/50 (72%)
Frame = -3 / +2
Query: 669 PVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR*Q 520
PV+Y H KF Q GC + YYTAHYQSP+ M+CLW KL G A CCN R*Q
Sbjct: 227 PVEYLHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR*Q 376
Score = 33.1 bits (66), Expect(2) = 9e-021
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -3 / +2
Query: 717 EDVSMGLWVEKFNST 673
EDVSMG+WV+KF+ T
Sbjct: 176 EDVSMGMWVQKFSKT 220
>gb|BJ253760.1|BJ253760 BJ253760 Y. Ogihara unpublished cDNA library, Wh_f Triticum
aestivum cDNA clone whf3c24 3', mRNA sequence
Length = 589
Score = 87.7 bits (185), Expect(2) = 9e-021
Identities = 32/50 (64%), Positives = 36/50 (72%)
Frame = -3 / -1
Query: 669 PVQYSHSWKFCQYGCLENYYTAHYQSPRQMLCLWDKLVRGQASCCNYR*Q 520
PV+Y H KF Q GC + YYTAHYQSP+ M+CLW KL G A CCN R*Q
Sbjct: 400 PVEYLHDVKFFQAGCFDGYYTAHYQSPQHMICLWRKLQSGSAQCCNAR*Q 251
Score = 33.1 bits (66), Expect(2) = 9e-021
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = -3 / -1
Query: 717 EDVSMGLWVEKFNST 673
EDVSMG+WV+KF+ T
Sbjct: 451 EDVSMGMWVQKFSKT 407
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 820,576,402
Number of Sequences: 636343
Number of extensions: 14078097
Number of successful extensions: 137324
Number of sequences better than 1.0e-020: 22
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 16673199992
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)