TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3336981.2.1
(510 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD884896.1|CD884896 F1.118B23F010510 F1 Triticum aestivu... 211 3e-063
gb|CN011458.1|CN011458 WHE3884_E06_I12ZS Wheat Fusarium gra... 170 8e-049
gb|AJ614344.1|AJ614344 AJ614344 Triticum turgidum subsp. du... 108 5e-042
gb|BM137376.1|BM137376 WHE0463-0466_C01_C01ZS Wheat Fusariu... 144 4e-041
gb|CD897506.1|CD897506 G174.106B19F010823 G174 Triticum aes... 109 4e-038
gb|AJ615866.1|AJ615866 AJ615866 Triticum turgidum subsp. du... 115 1e-037
gb|AL821028.1|AL821028 AL821028 O:232 Triticum aestivum cDN... 111 6e-024
gb|BG313979.1|BG313979 WHE2065_D11_H21ZS Wheat salt-stresse... 89 2e-023
gb|AL825764.1|AL825764 AL825764 p:234 Triticum aestivum cDN... 105 2e-023
gb|CK209928.1|CK209928 FGAS021717 Triticum aestivum FGAS: L... 103 1e-021
>gb|CD884896.1|CD884896 F1.118B23F010510 F1 Triticum aestivum cDNA clone F1118B23, mRNA
sequence
Length = 564
Score = 211 bits (456), Expect(2) = 3e-063
Identities = 86/142 (60%), Positives = 106/142 (74%)
Frame = +1 / +1
Query: 85 TYSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISAT 264
+Y SIQ+R ++M+ A LTFM+IGGFPSFVED+K+F +ERL+GHYGVS FVI+NT+SAT
Sbjct: 94 SYGSIQARGSMLMFVGAFLTFMAIGGFPSFVEDMKIFGRERLNGHYGVSSFVIANTVSAT 273
Query: 265 PYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGII 444
PYL +SL+PGAM YYL GL + D F YF +A+ VPDFLMGII
Sbjct: 274 PYLLLISLVPGAMAYYLVGLQRSFDQFAYFALVLFMTMMLVEGLMMIVASAVPDFLMGII 453
Query: 445 VGAGVQGVMMLNGGFFRLPNEL 510
GAG+QGVMMLNGGFFRLP++L
Sbjct: 454 TGAGIQGVMMLNGGFFRLPHDL 519
Score = 134 bits (287), Expect(2) = 1e-033
Identities = 65/125 (52%), Positives = 72/125 (57%)
Frame = -2 / -2
Query: 506 SLGRRKKPPLSIMTPCTPAPTMIPIRKSGTTAAMTIIMLXXXXXXXXXXXXXXXKWSAPL 327
S GRRKKPPLSIMTP PAP M+P+RKSGT A II WS L
Sbjct: 515 SWGRRKKPPLSIMTPWMPAPVMMPMRKSGTALATIIIRPSTSIMVMKSTSAK*ANWSKLL 336
Query: 326 DRPVR*YSIAPGSRETPARYGVAEMVLEMTNSDTP*WPXXXXXXXXXXXXTKDGNPPIDM 147
RP R* +IAPG+RE RYGVAE VL +TN +TP* P TKDG PP+ M
Sbjct: 335 CRPTR**AIAPGTREIRRRYGVAETVLAITNDETP*CPFSLSLPNIFMSSTKDGKPPMAM 156
Query: 146 KVRSA 132
KVR A
Sbjct: 155 KVRKA 141
Score = 51.0 bits (105), Expect(2) = 3e-063
Identities = 16/28 (57%), Positives = 23/28 (82%)
Frame = +1 / +1
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLGYS 84
+GYYW+R AIY+ + +C GTIFY +G+S
Sbjct: 13 LGYYWLRFAIYIALCLCCGTIFYDIGHS 96
Score = 29.0 bits (57), Expect(2) = 1e-033
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -1 / -1
Query: 84 AVAKLVEDSA*AHANAHVDGQTHPVV 7
A+A ++ED A A A VD + PVV
Sbjct: 96 AMADVIEDGAAAEAEGDVDSEAEPVV 19
>gb|CN011458.1|CN011458 WHE3884_E06_I12ZS Wheat Fusarium graminearum infected spike cDNA
library Triticum aestivum cDNA clone WHE3884_E06_I12,
mRNA sequence
Length = 736
Score = 170 bits (365), Expect(2) = 8e-049
Identities = 66/141 (46%), Positives = 95/141 (67%)
Frame = +1 / +1
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y+SI +R + +TFMSIGGFPSFVE++KVF++ERL+GHYGV+ FVI+NTISA P
Sbjct: 247 YTSILARAACAAFVFGFVTFMSIGGFPSFVEEMKVFQRERLNGHYGVAAFVIANTISALP 426
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIV 447
+L + + G + Y++ L G H+++F IA+V+P+FLMGII+
Sbjct: 427 FLVLICFMSGTVCYFMVRLHPGFTHYIFFVLNLYASVTVVESLMMAIASVIPNFLMGIII 606
Query: 448 GAGVQGVMMLNGGFFRLPNEL 510
GAG+QG+ ML G+FRLP ++
Sbjct: 607 GAGIQGIFMLVSGYFRLPYDI 669
Score = 44.1 bits (90), Expect(2) = 8e-049
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +1 / +1
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + VC+GTI+ +G
Sbjct: 166 GYYWLRLLIYLLVTVCIGTIYLDVG 240
>gb|AJ614344.1|AJ614344 AJ614344 Triticum turgidum subsp. durum etiolated seedling 20 day
Triticum turgidum subsp. durum cDNA clone 10080R, mRNA
sequence
Length = 602
Score = 108 bits (231), Expect(3) = 5e-042
Identities = 42/99 (42%), Positives = 62/99 (62%)
Frame = +1 / +3
Query: 214 GHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXX 393
G YGV+ FVI+NTISA P+L + + G + Y++ L G H+++F
Sbjct: 216 GTYGVAAFVIANTISALPFLVLICFMSGTVCYFMVRLHPGFTHYIFFVLNLYASVTVVES 395
Query: 394 XXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
IA+V+P+FLMGII+GAG+QG+ ML G+FRLP ++
Sbjct: 396 LMMAIASVIPNFLMGIIIGAGIQGIFMLVSGYFRLPYDI 512
Score = 66.1 bits (138), Expect(3) = 5e-042
Identities = 25/44 (56%), Positives = 34/44 (77%)
Frame = +1 / +2
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGH 219
Y+SI +R + +TFMSIGGFPSFVE++KVF++ERL+GH
Sbjct: 89 YTSILARAACAAFVFGFVTFMSIGGFPSFVEEMKVFQRERLNGH 220
Score = 35.0 bits (70), Expect(3) = 5e-042
Identities = 11/22 (50%), Positives = 17/22 (77%)
Frame = +1 / +2
Query: 13 WMRLAIYMGIGVCLGTIFYQLG 78
W+RL IY+ + VC+GTI+ +G
Sbjct: 17 WLRLLIYLLVTVCIGTIYLDVG 82
>gb|BM137376.1|BM137376 WHE0463-0466_C01_C01ZS Wheat Fusarium graminearum infected spike
cDNA library Triticum aestivum cDNA clone
WHE0463-0466_C01_C01, mRNA sequence
Length = 617
Score = 144 bits (309), Expect(2) = 4e-041
Identities = 57/123 (46%), Positives = 81/123 (65%)
Frame = +1 / +1
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y+SI +R + +TFMSIGGFPSFVE++KVF++ERL+GHYGV+ FVI+NTISA P
Sbjct: 247 YTSILARAACAAFVFGFVTFMSIGGFPSFVEEMKVFQRERLNGHYGVAAFVIANTISALP 426
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIV 447
+L + + G + Y++ L G H+++F IA+V+P+FLMGII+
Sbjct: 427 FLVLICFMSGTVCYFMVRLHPGFTHYIFFVLNLYASVTVVESLMMAIASVIPNFLMGIII 606
Query: 448 GAG 456
GAG
Sbjct: 607 GAG 615
Score = 44.1 bits (90), Expect(2) = 4e-041
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +1 / +1
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + VC+GTI+ +G
Sbjct: 166 GYYWLRLLIYLLVTVCIGTIYLDVG 240
>gb|CD897506.1|CD897506 G174.106B19F010823 G174 Triticum aestivum cDNA clone G174106B19,
mRNA sequence
Length = 500
Score = 109 bits (233), Expect(3) = 4e-038
Identities = 40/77 (51%), Positives = 58/77 (75%)
Frame = +1 / +3
Query: 124 YTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAM 303
+ +TFMSIGGFPSFVE++KVF++ERL+GHYGV+ FVI+NTISA P+L + + G +
Sbjct: 108 FVFGFVTFMSIGGFPSFVEEMKVFQRERLNGHYGVAAFVIANTISALPFLVLICFMSGTV 287
Query: 304 LYYLTGLSKGADHFVYF 354
Y++ L G H+++F
Sbjct: 288 CYFMVRLHPGFTHYIFF 338
Score = 61.6 bits (128), Expect(3) = 4e-038
Identities = 22/35 (62%), Positives = 31/35 (88%)
Frame = +1 / +2
Query: 406 IAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
IA+V+P+FLMGII+GAG+QG+ ML G+FRLP ++
Sbjct: 389 IASVIPNFLMGIIIGAGIQGIFMLVSGYFRLPYDI 493
Score = 25.4 bits (49), Expect(3) = 4e-038
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +1 / +2
Query: 28 IYMGIGVCLGTIFYQLG 78
IY+ + VC+GTI+ +G
Sbjct: 14 IYLLVTVCIGTIYLDVG 64
>gb|AJ615866.1|AJ615866 AJ615866 Triticum turgidum subsp. durum etiolated seedling 20 day
Triticum turgidum subsp. durum cDNA clone 12297R, mRNA
sequence
Length = 682
Score = 115 bits (245), Expect(2) = 1e-037
Identities = 43/89 (48%), Positives = 63/89 (70%)
Frame = +1 / +2
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y+SI +R + +TFMSIGGFPSFVE++KVF++ERL+GHYGV+ FVI+NTIS P
Sbjct: 35 YTSILARAACAAFVFGFVTFMSIGGFPSFVEEMKVFQRERLNGHYGVAAFVIANTISXLP 214
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYF 354
+L + + G + Y++ L G H+++F
Sbjct: 215 FLVLICFMSGTVCYFMVRLHPGFTHYIFF 301
Score = 61.6 bits (128), Expect(2) = 1e-037
Identities = 22/35 (62%), Positives = 31/35 (88%)
Frame = +1 / +3
Query: 406 IAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
IA+V+P+FLMGII+GAG+QG+ ML G+FRLP ++
Sbjct: 354 IASVIPNFLMGIIIGAGIQGIFMLVSGYFRLPYDI 458
>gb|AL821028.1|AL821028 AL821028 O:232 Triticum aestivum cDNA clone F08_O232_plate_7, mRNA
sequence
Length = 508
Score = 111 bits (237), Expect = 6e-024
Identities = 43/99 (43%), Positives = 62/99 (62%)
Frame = +1 / +3
Query: 214 GHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXX 393
GHYGV+ FVI+NTISA P+L + + G + Y++ L G H+++F
Sbjct: 9 GHYGVAAFVIANTISALPFLVLICFMSGTVCYFMVRLHPGFTHYIFFVLNLYASVTVVES 188
Query: 394 XXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
IA+V+P FLMGII+GAG+QG+ ML G+FRLP ++
Sbjct: 189 LMMAIASVIPYFLMGIIIGAGIQGIFMLVSGYFRLPYDI 305
>gb|BG313979.1|BG313979 WHE2065_D11_H21ZS Wheat salt-stressed sheath cDNA library Triticum
aestivum cDNA clone WHE2065_D11_H21, mRNA sequence
Length = 592
Score = 88.6 bits (187), Expect(2) = 2e-023
Identities = 34/75 (45%), Positives = 53/75 (70%)
Frame = +1 / +3
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y++IQ+R + +TFMSIGGFPSF+E++KVF ER +GHYGV+ ++ISN +S+ P
Sbjct: 366 YTAIQARASCGGFVSGFMTFMSIGGFPSFIEEMKVFSLERQNGHYGVAAYIISNFLSSMP 545
Query: 268 YLAGVSLLPGAMLYY 312
+L +S ++ Y+
Sbjct: 546 FLLTMSWASASITYW 590
Score = 40.5 bits (82), Expect(2) = 2e-023
Identities = 13/25 (52%), Positives = 20/25 (80%)
Frame = +1 / +3
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYY +R+ IY+ + +CLGTI+Y +G
Sbjct: 285 GYYRLRIIIYVLMAICLGTIYYDVG 359
>gb|AL825764.1|AL825764 AL825764 p:234 Triticum aestivum cDNA clone H11_p234_plate_15, mRNA
sequence
Length = 526
Score = 105 bits (224), Expect = 4e-022
Identities = 49/73 (67%), Positives = 50/73 (68%)
Frame = -2 / -1
Query: 509 SSLGRRKKPPLSIMTPCTPAPTMIPIRKSGTTAAMTIIMLXXXXXXXXXXXXXXXKWSAP 330
SSLGRRKKPPLSI+TPCTPAPTMIPIRKSGT AM IIML K S P
Sbjct: 226 SSLGRRKKPPLSIITPCTPAPTMIPIRKSGTMTAMIIIMLSTSSVHMHRLTTK*AKRSGP 47
Query: 329 LDRPVR*YSIAPG 291
PVR*YSIAPG
Sbjct: 46 FVSPVR*YSIAPG 8
Score = 81.3 bits (171), Expect(2) = 2e-023
Identities = 33/35 (94%), Positives = 34/35 (97%)
Frame = +1 / +3
Query: 406 IAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
IA +VPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL
Sbjct: 123 IAVIVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 227
Score = 47.8 bits (98), Expect(2) = 2e-023
Identities = 17/23 (73%), Positives = 20/23 (86%)
Frame = +1 / +3
Query: 286 LLPGAMLYYLTGLSKGADHFVYF 354
++PGAMLYYLTGL+KG D F YF
Sbjct: 3 VIPGAMLYYLTGLTKGPDRFAYF 71
>gb|CK209928.1|CK209928 FGAS021717 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 998
Score = 103 bits (220), Expect = 1e-021
Identities = 44/108 (40%), Positives = 65/108 (60%)
Frame = +1 / +3
Query: 187 KVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXX 366
+VF ER +GHYGV+ ++ISN +S+ P+L +S ++ Y++ G +F +F
Sbjct: 21 EVFSLERQNGHYGVAAYIISNFLSSMPFLLTMSWASASITYWMVKFRPGFSYFAFFALNL 200
Query: 367 XXXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
I+A+VP+FLMG+I+GAGV G+MML GFFRL EL
Sbjct: 201 YGGVSVIESLMMIISALVPNFLMGLILGAGVIGIMMLTSGFFRLLPEL 344
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 420,532,197
Number of Sequences: 636343
Number of extensions: 5179862
Number of successful extensions: 146420
Number of sequences better than 1.0e-020: 12
length of database: 122,413,413
effective HSP length: 52
effective length of database: 89,323,577
effective search space used: 10450858509
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)