TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115079.2.1
(636 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ216979.1|BJ216979 BJ216979 Y. Ogihara unpublished cDNA... 250 9e-066
gb|CK205864.1|CK205864 FGAS017413 Triticum aestivum FGAS: L... 249 2e-065
gb|CK205437.1|CK205437 FGAS016922 Triticum aestivum FGAS: L... 246 2e-064
gb|BJ216653.1|BJ216653 BJ216653 Y. Ogihara unpublished cDNA... 228 4e-059
gb|BF484118.1|BF484118 WHE1788_E07_I14ZS Wheat pre-anthesis... 221 8e-057
gb|BJ209232.1|BJ209232 BJ209232 Y. Ogihara unpublished cDNA... 218 6e-056
gb|CA502438.1|CA502438 WHE4047_E05_J09ZT Wheat meiotic anth... 82 4e-024
gb|CA594953.1|CA594953 wpa1c.pk006.e16 wpa1c Triticum aesti... 82 4e-024
>gb|BJ216979.1|BJ216979 BJ216979 Y. Ogihara unpublished cDNA library, Wh Triticum aestivum
cDNA clone wh14j12 3', mRNA sequence
Length = 642
Score = 250 bits (541), Expect = 9e-066
Identities = 100/112 (89%), Positives = 109/112 (97%)
Frame = -2 / -2
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
LPEKIESRIEGFCR LM++VGIK+FND+FWAVHPGGPAILNRLE CL+L+P+KLKISRKA
Sbjct: 545 LPEKIESRIEGFCRILMDKVGIKEFNDVFWAVHPGGPAILNRLEVCLQLEPDKLKISRKA 366
Query: 455 LMNYGNVSSNTIFYVLEYLRDELKKGAISEEWGLILAFGPGITFEGLLVRGV 300
LMNYGNVSSNT+FYVLEYLRDELKKGAI EEWGLILAFGPGITFEGLLVRG+
Sbjct: 365 LMNYGNVSSNTVFYVLEYLRDELKKGAIREEWGLILAFGPGITFEGLLVRGI 210
Score = 214 bits (462), Expect(2) = 3e-056
Identities = 83/109 (76%), Positives = 91/109 (83%)
Frame = +3 / +3
Query: 309 N*QSFKGDAWAKSQDQSPLFTYCPFL*LVPQVLQHIEDCIAAHISIVH*GFSANLELLWL 488
N*QSFK DAWAKSQDQSPLF YCPFL +PQ+LQHIEDC+ AHI IVH G S +LE +WL
Sbjct: 219 N*QSFKCDAWAKSQDQSPLFPYCPFLQFIPQILQHIEDCVTAHIPIVHQGLSTDLEFIWL 398
Query: 489 KFKAELKPVQYCWSTRMHSPKYIVEILYPYLVHESPAETFYAAFNLLRQ 635
K K L+PVQYCWST MHSPKYI+E+LYP LVHE PAE FYAAFNL RQ
Sbjct: 399 KLKTNLQPVQYCWSTGMHSPKYIIELLYPNLVHEYPAEAFYAAFNLFRQ 545
Score = 201 bits (433), Expect = 7e-051
Identities = 90/118 (76%), Positives = 94/118 (79%)
Frame = +1 / +1
Query: 283 LVKVFFTPRTSSPSKVMPGPKAKINPHSSLIAPFFNSSLKYSST*KIVLLLTFP*FIKAX 462
L++ PRTSSPS VMPGPKAKINPHSSLIAPFFNSSLKYSST*K VLLLTFP*FI+A
Sbjct: 193 LLQFQLMPRTSSPSNVMPGPKAKINPHSSLIAPFFNSSLKYSST*KTVLLLTFP*FIRAF 372
Query: 463 XXXXXXXXXXXRQNSSLFNIAGPPGCTAQNISLKSFIPTWFMRVLQKPSMRLSIFSGK 636
RQ SSLFNIAGPPGCTAQN SL SFIPT M +LQKPSMRLSIFSGK
Sbjct: 373 LLILSLSGSS*RQTSSLFNIAGPPGCTAQNTSLNSFIPTLSMSILQKPSMRLSIFSGK 546
Score = 173 bits (373), Expect = 1e-042
Identities = 78/110 (70%), Positives = 87/110 (79%)
Frame = -1 / -1
Query: 636 FA*ED*KPHRRFLQDSHEPGRDKGFQRYILGCASWWTSNIEQA*VLP*TSAREAQD*QKS 457
FA*+D*KPHRR LQD+H G DKG Q ILGCAS WTSNIEQA L T AR QD K
Sbjct: 546 FA*KD*KPHRRLLQDTHGQGWDKGVQ*CILGCASRWTSNIEQAGGLSST*AR*TQDQ*KG 367
Query: 456 LNELWKCEQQYNLLCAGVLEGRVKERGNK*RVGIDLGFWPRHHL*RTASS 307
+ELW+CEQ ++LLCAGV EG ++ERGNK RVGIDLGFWPRHH+*RTASS
Sbjct: 366 PDELWECEQ*HSLLCAGVFEG*IEERGNKGRVGIDLGFWPRHHI*RTASS 217
Score = 165 bits (355), Expect = 4e-040
Identities = 81/109 (74%), Positives = 86/109 (78%)
Frame = -3 / -3
Query: 634 CLRRLKAA*KVSAGLS*TR*G*RISTIYFGLCILVDQQY*TGLSSALNFSQRSSRLAEKP 455
CL+RLKAA*K SAG S TR G*R S +YFGLCI VDQQY*TG N SQ +SR E+P
Sbjct: 544 CLKRLKAA*KASAGYSWTRLG*RSSMMYFGLCIPVDQQY*TGWRFVFNLSQINSRSVERP 365
Query: 454 **TMEM*AAIQSSMCWST*GTS*RKGQ*VKSGD*SWLLAQASPLKDC*F 308
**TM M*A QSSMCWS *G +*RKGQ* KSGD*SWLLAQAS LKDC*F
Sbjct: 364 **TMGM*AVTQSSMCWSI*GMN*RKGQ*GKSGD*SWLLAQASHLKDC*F 218
Score = 104 bits (222), Expect(2) = 5e-026
Identities = 50/75 (66%), Positives = 55/75 (73%)
Frame = +2 / +2
Query: 410 AHRRLYCCSHFHSSLRLFC*S*ASLAEV*GRTQACSILLVHQDAQPKIYR*NPLSLPGS* 589
AHRRL CSH HSS F *S* LA+V + ACSILLVH+DAQPKI+ * PLS P *
Sbjct: 320 AHRRLCYCSHSHSSSGPFY*S*VYLAQVEDKPPACSILLVHRDAQPKIHH*TPLSQPCP* 499
Query: 590 ESCRNLLCGFQSSQA 634
SCR+LLCGFQS QA
Sbjct: 500 VSCRSLLCGFQSFQA 544
Score = 33.6 bits (67), Expect(2) = 5e-026
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = +2 / +2
Query: 308 ELAVLQR*CLGQKPR 352
ELAVLQ *CLGQKPR
Sbjct: 218 ELAVLQM*CLGQKPR 262
Score = 24.9 bits (48), Expect(2) = 3e-056
Identities = 7/21 (33%), Positives = 15/21 (71%)
Frame = +3 / +1
Query: 192 QLPPRHLCSLCDVSVYIINLY 254
++ P H+ +CD+S+Y++ Y
Sbjct: 124 EMLPCHVKLVCDLSIYLVVTY 186
>gb|CK205864.1|CK205864 FGAS017413 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1077
Score = 249 bits (538), Expect = 2e-065
Identities = 100/111 (90%), Positives = 108/111 (97%)
Frame = -2 / -3
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
LPEKIESRIEGFCR LM++VGIK+FND+FWAVHPGGPAILNRLE CL+L+P+KLKISRKA
Sbjct: 613 LPEKIESRIEGFCRILMDKVGIKEFNDVFWAVHPGGPAILNRLEVCLQLEPDKLKISRKA 434
Query: 455 LMNYGNVSSNTIFYVLEYLRDELKKGAISEEWGLILAFGPGITFEGLLVRG 303
LMNYGNVSSNT+FYVLEYLRDELKKGAI EEWGLILAFGPGITFEGLLVRG
Sbjct: 433 LMNYGNVSSNTVFYVLEYLRDELKKGAIREEWGLILAFGPGITFEGLLVRG 281
Score = 215 bits (465), Expect(2) = 1e-056
Identities = 83/113 (73%), Positives = 93/113 (82%)
Frame = +3 / +2
Query: 297 LYTSN*QSFKGDAWAKSQDQSPLFTYCPFL*LVPQVLQHIEDCIAAHISIVH*GFSANLE 476
++ N*QSFK DAWAKSQDQSPLF YCPFL +PQ+LQHIEDC+ AHI IVH G S +LE
Sbjct: 275 VFAPN*QSFKCDAWAKSQDQSPLFPYCPFLQFIPQILQHIEDCVTAHIPIVHQGLSTDLE 454
Query: 477 LLWLKFKAELKPVQYCWSTRMHSPKYIVEILYPYLVHESPAETFYAAFNLLRQ 635
+WLK K L+PVQYCWST MHSPKYI+E+LYP LVHE PAE FYAAFNL RQ
Sbjct: 455 FIWLKLKTNLQPVQYCWSTGMHSPKYIIELLYPNLVHEYPAEAFYAAFNLFRQ 613
Score = 204 bits (439), Expect = 1e-051
Identities = 91/118 (77%), Positives = 95/118 (80%)
Frame = +1 / +3
Query: 283 LVKVFFTPRTSSPSKVMPGPKAKINPHSSLIAPFFNSSLKYSST*KIVLLLTFP*FIKAX 462
L++ F PRTSSPS VMPGPKAKINPHSSLIAPFFNSSLKYSST*K VLLLTFP*FI+A
Sbjct: 261 LLQFQFLPRTSSPSNVMPGPKAKINPHSSLIAPFFNSSLKYSST*KTVLLLTFP*FIRAF 440
Query: 463 XXXXXXXXXXXRQNSSLFNIAGPPGCTAQNISLKSFIPTWFMRVLQKPSMRLSIFSGK 636
RQ SSLFNIAGPPGCTAQN SL SFIPT M +LQKPSMRLSIFSGK
Sbjct: 441 LLILSLSGSS*RQTSSLFNIAGPPGCTAQNTSLNSFIPTLSMSILQKPSMRLSIFSGK 614
Score = 175 bits (376), Expect = 5e-043
Identities = 80/119 (67%), Positives = 91/119 (76%)
Frame = -1 / -2
Query: 636 FA*ED*KPHRRFLQDSHEPGRDKGFQRYILGCASWWTSNIEQA*VLP*TSAREAQD*QKS 457
FA*+D*KPHRR LQD+H G DKG Q ILGCAS WTSNIEQA L T AR QD K
Sbjct: 614 FA*KD*KPHRRLLQDTHGQGWDKGVQ*CILGCASRWTSNIEQAGGLSST*AR*TQDQ*KG 435
Query: 456 LNELWKCEQQYNLLCAGVLEGRVKERGNK*RVGIDLGFWPRHHL*RTASSRCKENLHKL 280
+ELW+CEQ ++LLCAGV EG ++ERGNK RVGIDLGFWPRHH+*RTASS K L ++
Sbjct: 434 PDELWECEQ*HSLLCAGVFEG*IEERGNKGRVGIDLGFWPRHHI*RTASSGQKLKLKEI 258
Score = 165 bits (355), Expect = 4e-040
Identities = 81/109 (74%), Positives = 86/109 (78%)
Frame = -3 / -1
Query: 634 CLRRLKAA*KVSAGLS*TR*G*RISTIYFGLCILVDQQY*TGLSSALNFSQRSSRLAEKP 455
CL+RLKAA*K SAG S TR G*R S +YFGLCI VDQQY*TG N SQ +SR E+P
Sbjct: 612 CLKRLKAA*KASAGYSWTRLG*RSSMMYFGLCIPVDQQY*TGWRFVFNLSQINSRSVERP 433
Query: 454 **TMEM*AAIQSSMCWST*GTS*RKGQ*VKSGD*SWLLAQASPLKDC*F 308
**TM M*A QSSMCWS *G +*RKGQ* KSGD*SWLLAQAS LKDC*F
Sbjct: 432 **TMGM*AVTQSSMCWSI*GMN*RKGQ*GKSGD*SWLLAQASHLKDC*F 286
Score = 104 bits (222), Expect(2) = 2e-026
Identities = 50/75 (66%), Positives = 55/75 (73%)
Frame = +2 / +1
Query: 410 AHRRLYCCSHFHSSLRLFC*S*ASLAEV*GRTQACSILLVHQDAQPKIYR*NPLSLPGS* 589
AHRRL CSH HSS F *S* LA+V + ACSILLVH+DAQPKI+ * PLS P *
Sbjct: 388 AHRRLCYCSHSHSSSGPFY*S*VYLAQVEDKPPACSILLVHRDAQPKIHH*TPLSQPCP* 567
Query: 590 ESCRNLLCGFQSSQA 634
SCR+LLCGFQS QA
Sbjct: 568 VSCRSLLCGFQSFQA 612
Score = 35.4 bits (71), Expect(2) = 2e-026
Identities = 16/20 (80%), Positives = 16/20 (80%)
Frame = +2 / +1
Query: 293 FSLHLELAVLQR*CLGQKPR 352
FS ELAVLQ *CLGQKPR
Sbjct: 271 FSFCPELAVLQM*CLGQKPR 330
Score = 24.9 bits (48), Expect(2) = 1e-056
Identities = 7/21 (33%), Positives = 15/21 (71%)
Frame = +3 / +3
Query: 192 QLPPRHLCSLCDVSVYIINLY 254
++ P H+ +CD+S+Y++ Y
Sbjct: 192 EMLPCHVKLVCDLSIYLVVTY 254
>gb|CK205437.1|CK205437 FGAS016922 Triticum aestivum FGAS: Library 5 GATE 7 Triticum
aestivum cDNA, mRNA sequence
Length = 1063
Score = 246 bits (531), Expect = 2e-064
Identities = 98/112 (87%), Positives = 108/112 (96%)
Frame = -2 / -3
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
LPEKIESRIEGFCR LM++VGIK+FND+FWAVHPGGPAILNRLE CL+L+P+KLKISRKA
Sbjct: 611 LPEKIESRIEGFCRILMDKVGIKEFNDVFWAVHPGGPAILNRLEVCLELEPDKLKISRKA 432
Query: 455 LMNYGNVSSNTIFYVLEYLRDELKKGAISEEWGLILAFGPGITFEGLLVRGV 300
LMNYGNVSSNT+FYVLEYLRDELKKGAI EEWGLILAFGPGITFEG+LVR +
Sbjct: 431 LMNYGNVSSNTVFYVLEYLRDELKKGAIREEWGLILAFGPGITFEGMLVRRI 276
Score = 218 bits (471), Expect = 4e-056
Identities = 85/109 (77%), Positives = 93/109 (85%)
Frame = +3 / +3
Query: 309 N*QSFKGDAWAKSQDQSPLFTYCPFL*LVPQVLQHIEDCIAAHISIVH*GFSANLELLWL 488
N* SFK DAWAKSQDQSPLF YCPFL L+PQ+LQHIEDC+AAHI IVH G S +LE +WL
Sbjct: 285 N*HSFKCDAWAKSQDQSPLFPYCPFLQLIPQILQHIEDCVAAHIPIVHQGLSTDLEFIWL 464
Query: 489 KFKAELKPVQYCWSTRMHSPKYIVEILYPYLVHESPAETFYAAFNLLRQ 635
KFK L+PVQYCWST MHSPKYI+E+LYP LVHE PAE FYAAFNL RQ
Sbjct: 465 KFKTNLQPVQYCWSTGMHSPKYIIELLYPNLVHEYPAEAFYAAFNLFRQ 611
Score = 196 bits (423), Expect = 2e-049
Identities = 87/110 (79%), Positives = 89/110 (80%)
Frame = +1 / +1
Query: 307 RTSSPSKVMPGPKAKINPHSSLIAPFFNSSLKYSST*KIVLLLTFP*FIKAXXXXXXXXX 486
RTS PS VMPGPKAKINPHSSLIAPFFNSSLKYSST*K VLLLTFP*FI+A
Sbjct: 283 RTSIPSNVMPGPKAKINPHSSLIAPFFNSSLKYSST*KTVLLLTFP*FIRAFLLILSLSG 462
Query: 487 XXXRQNSSLFNIAGPPGCTAQNISLKSFIPTWFMRVLQKPSMRLSIFSGK 636
RQ SSLFNIAGPPGCTAQN SL SFIPT M +LQKPSMRLSIFSGK
Sbjct: 463 SSSRQTSSLFNIAGPPGCTAQNTSLNSFIPTLSMSILQKPSMRLSIFSGK 612
Score = 178 bits (383), Expect = 6e-044
Identities = 80/110 (72%), Positives = 88/110 (80%)
Frame = -1 / -2
Query: 636 FA*ED*KPHRRFLQDSHEPGRDKGFQRYILGCASWWTSNIEQA*VLP*TSAREAQD*QKS 457
FA*+D*KPHRR LQD+H G DKG Q ILGCAS WTSNIEQA L *T AR QD K
Sbjct: 612 FA*KD*KPHRRLLQDTHGQGWDKGVQ*CILGCASRWTSNIEQAGGLS*T*AR*TQDQ*KG 433
Query: 456 LNELWKCEQQYNLLCAGVLEGRVKERGNK*RVGIDLGFWPRHHL*RTASS 307
+ELW+CEQQ++LLCAGV EG V+ERGNK RVGIDLGFWPRHH+*R ASS
Sbjct: 432 PDELWECEQQHSLLCAGVFEG*VEERGNKGRVGIDLGFWPRHHI*RNASS 283
Score = 168 bits (362), Expect = 4e-041
Identities = 83/109 (76%), Positives = 88/109 (80%)
Frame = -3 / -1
Query: 634 CLRRLKAA*KVSAGLS*TR*G*RISTIYFGLCILVDQQY*TGLSSALNFSQRSSRLAEKP 455
CL+RLKAA*K SAG S TR G*R S +YFGLCI VDQQY*TG LN SQ +SR E+P
Sbjct: 610 CLKRLKAA*KASAGYSWTRLG*RSSMMYFGLCIPVDQQY*TGWRFVLNLSQINSRSVERP 431
Query: 454 **TMEM*AAIQSSMCWST*GTS*RKGQ*VKSGD*SWLLAQASPLKDC*F 308
**TM M*AA QSSMCWS *G S*RKGQ* KSGD*SWLLAQAS LK+C*F
Sbjct: 430 **TMGM*AATQSSMCWSI*GMS*RKGQ*GKSGD*SWLLAQASHLKEC*F 284
Score = 109 bits (233), Expect(2) = 8e-027
Identities = 51/75 (68%), Positives = 56/75 (74%)
Frame = +2 / +2
Query: 410 AHRRLYCCSHFHSSLRLFC*S*ASLAEV*GRTQACSILLVHQDAQPKIYR*NPLSLPGS* 589
AHRRL CCSH HSS F *S* LA+V + ACSILLVH+DAQPKI+ * PLS P *
Sbjct: 386 AHRRLCCCSHSHSSSGPFY*S*VYLAQVQDKPPACSILLVHRDAQPKIHH*TPLSQPCP* 565
Query: 590 ESCRNLLCGFQSSQA 634
SCR+LLCGFQS QA
Sbjct: 566 VSCRSLLCGFQSFQA 610
Score = 31.3 bits (62), Expect(2) = 8e-027
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +2 / +2
Query: 308 ELAVLQR*CLGQKPR 352
ELA LQ *CLGQKPR
Sbjct: 284 ELAFLQM*CLGQKPR 328
>gb|BJ216653.1|BJ216653 BJ216653 Y. Ogihara unpublished cDNA library, Wh Triticum aestivum
cDNA clone wh13d20 3', mRNA sequence
Length = 543
Score = 228 bits (493), Expect = 4e-059
Identities = 90/102 (88%), Positives = 99/102 (97%)
Frame = -2 / -1
Query: 605 GFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSN 426
GFCR LM++VGIK+FND+FWAVHPGGPAILNRLE CL+L+P+KLKISRKALMNYGNVSSN
Sbjct: 543 GFCRILMDKVGIKEFNDVFWAVHPGGPAILNRLEVCLQLEPDKLKISRKALMNYGNVSSN 364
Query: 425 TIFYVLEYLRDELKKGAISEEWGLILAFGPGITFEGLLVRGV 300
T+FYVLEYLRDELKKGAI EEWGLILAFGPGITFEGLLVRG+
Sbjct: 363 TVFYVLEYLRDELKKGAIREEWGLILAFGPGITFEGLLVRGI 238
Score = 193 bits (415), Expect(2) = 9e-050
Identities = 74/98 (75%), Positives = 82/98 (83%)
Frame = +3 / +1
Query: 309 N*QSFKGDAWAKSQDQSPLFTYCPFL*LVPQVLQHIEDCIAAHISIVH*GFSANLELLWL 488
N*QSFK DAWAKSQDQSPLF YCPFL +PQ+LQHIEDC+ AHI IVH G S +LE +WL
Sbjct: 247 N*QSFKCDAWAKSQDQSPLFPYCPFLQFIPQILQHIEDCVTAHIPIVHQGLSTDLEFIWL 426
Query: 489 KFKAELKPVQYCWSTRMHSPKYIVEILYPYLVHESPAE 602
K K L+PVQYCWST MHSPKYI+E+LYP LVHE PAE
Sbjct: 427 KLKTNLQPVQYCWSTGMHSPKYIIELLYPNLVHEYPAE 540
Score = 176 bits (379), Expect = 2e-043
Identities = 79/107 (73%), Positives = 83/107 (77%)
Frame = +1 / +2
Query: 283 LVKVFFTPRTSSPSKVMPGPKAKINPHSSLIAPFFNSSLKYSST*KIVLLLTFP*FIKAX 462
L++ PRTSSPS VMPGPKAKINPHSSLIAPFFNSSLKYSST*K VLLLTFP*FI+A
Sbjct: 221 LLQFQLMPRTSSPSNVMPGPKAKINPHSSLIAPFFNSSLKYSST*KTVLLLTFP*FIRAF 400
Query: 463 XXXXXXXXXXXRQNSSLFNIAGPPGCTAQNISLKSFIPTWFMRVLQK 603
RQ SSLFNIAGPPGCTAQN SL SFIPT M +LQK
Sbjct: 401 LLILSLSGSS*RQTSSLFNIAGPPGCTAQNTSLNSFIPTLSMSILQK 541
Score = 151 bits (324), Expect = 8e-036
Identities = 68/98 (69%), Positives = 76/98 (77%)
Frame = -1 / -3
Query: 600 LQDSHEPGRDKGFQRYILGCASWWTSNIEQA*VLP*TSAREAQD*QKSLNELWKCEQQYN 421
LQD+H G DKG Q ILGCAS WTSNIEQA L T AR QD K +ELW+CEQ ++
Sbjct: 538 LQDTHGQGWDKGVQ*CILGCASRWTSNIEQAGGLSST*AR*TQDQ*KGPDELWECEQ*HS 359
Query: 420 LLCAGVLEGRVKERGNK*RVGIDLGFWPRHHL*RTASS 307
LLCAGV EG ++ERGNK RVGIDLGFWPRHH+*RTASS
Sbjct: 358 LLCAGVFEG*IEERGNKGRVGIDLGFWPRHHI*RTASS 245
Score = 145 bits (312), Expect = 3e-034
Identities = 72/98 (73%), Positives = 76/98 (77%)
Frame = -3 / -2
Query: 601 SAGLS*TR*G*RISTIYFGLCILVDQQY*TGLSSALNFSQRSSRLAEKP**TMEM*AAIQ 422
SAG S TR G*R S +YFGLCI VDQQY*TG N SQ +SR E+P**TM M*A Q
Sbjct: 539 SAGYSWTRLG*RSSMMYFGLCIPVDQQY*TGWRFVFNLSQINSRSVERP**TMGM*AVTQ 360
Query: 421 SSMCWST*GTS*RKGQ*VKSGD*SWLLAQASPLKDC*F 308
SSMCWS *G +*RKGQ* KSGD*SWLLAQAS LKDC*F
Sbjct: 359 SSMCWSI*GMN*RKGQ*GKSGD*SWLLAQASHLKDC*F 246
Score = 24.9 bits (48), Expect(2) = 9e-050
Identities = 7/21 (33%), Positives = 15/21 (71%)
Frame = +3 / +2
Query: 192 QLPPRHLCSLCDVSVYIINLY 254
++ P H+ +CD+S+Y++ Y
Sbjct: 152 EMLPCHVKLVCDLSIYLVVTY 214
>gb|BF484118.1|BF484118 WHE1788_E07_I14ZS Wheat pre-anthesis spike cDNA library Triticum
aestivum cDNA clone WHE1788_E07_I14, mRNA sequence
Length = 677
Score = 221 bits (476), Expect = 8e-057
Identities = 88/99 (88%), Positives = 96/99 (96%)
Frame = -2 / +1
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
LPEKIESRIEGFCR LM++VGIK+FND+FWAVHPGGPAILNRLE CL+L+P+KLKISRKA
Sbjct: 379 LPEKIESRIEGFCRILMDKVGIKEFNDVFWAVHPGGPAILNRLEVCLELEPDKLKISRKA 558
Query: 455 LMNYGNVSSNTIFYVLEYLRDELKKGAISEEWGLILAFG 339
LMNYGNVSSNT+FYVLEYLRDELKKGAI EEWGLILAFG
Sbjct: 559 LMNYGNVSSNTVFYVLEYLRDELKKGAIREEWGLILAFG 675
Score = 193 bits (417), Expect = 1e-048
Identities = 73/99 (73%), Positives = 83/99 (83%)
Frame = +3 / -3
Query: 339 AKSQDQSPLFTYCPFL*LVPQVLQHIEDCIAAHISIVH*GFSANLELLWLKFKAELKPVQ 518
AKS+DQSPLF YCPFL +PQ+LQHIEDC+AAHI IVH G S +LE +WLKFK L+PVQ
Sbjct: 675 AKSKDQSPLFPYCPFLQFIPQILQHIEDCVAAHIPIVHQGLSTDLEFIWLKFKTNLQPVQ 496
Query: 519 YCWSTRMHSPKYIVEILYPYLVHESPAETFYAAFNLLRQ 635
YCWS MHSPKY++E+LYP LVHE PAE FYAAFNL RQ
Sbjct: 495 YCWSAGMHSPKYVIELLYPNLVHEYPAEAFYAAFNLFRQ 379
Score = 177 bits (381), Expect = 1e-043
Identities = 78/100 (78%), Positives = 81/100 (81%)
Frame = +1 / -1
Query: 337 GPKAKINPHSSLIAPFFNSSLKYSST*KIVLLLTFP*FIKAXXXXXXXXXXXXRQNSSLF 516
GPKA+INPHSSLIAPFFNSSLKYSST*K VLLLTFP*FI+A RQ SSLF
Sbjct: 677 GPKARINPHSSLIAPFFNSSLKYSST*KTVLLLTFP*FIRAFLLILSLSGSSSRQTSSLF 498
Query: 517 NIAGPPGCTAQNISLKSFIPTWFMRVLQKPSMRLSIFSGK 636
NIAGPPGCTAQN SL SFIPT M +LQKPSMRLSIFSGK
Sbjct: 497 NIAGPPGCTAQNTSLNSFIPTLSMSILQKPSMRLSIFSGK 378
Score = 145 bits (311), Expect = 5e-034
Identities = 68/100 (68%), Positives = 76/100 (76%)
Frame = -1 / +3
Query: 636 FA*ED*KPHRRFLQDSHEPGRDKGFQRYILGCASWWTSNIEQA*VLP*TSAREAQD*QKS 457
FA*+D*KPHRR LQD+H G DKG Q ILGCAS TSNIEQA L *T AR QD K
Sbjct: 378 FA*KD*KPHRRLLQDTHGQGWDKGVQ*RILGCASRRTSNIEQAGGLS*T*AR*TQDQ*KG 557
Query: 456 LNELWKCEQQYNLLCAGVLEGRVKERGNK*RVGIDLGFWP 337
+ELW+CEQQ++LLCAGV EG ++ERGNK RVGID FWP
Sbjct: 558 PDELWECEQQHSLLCAGVFEG*IEERGNKGRVGIDPCFWP 677
Score = 141 bits (302), Expect = 8e-033
Identities = 72/99 (72%), Positives = 76/99 (76%)
Frame = -3 / +2
Query: 634 CLRRLKAA*KVSAGLS*TR*G*RISTIYFGLCILVDQQY*TGLSSALNFSQRSSRLAEKP 455
CL+RLKAA*K SAG S TR G*R S YFGLCI DQQY*TG LN SQ +SR E+P
Sbjct: 380 CLKRLKAA*KASAGYSWTRLG*RSSMTYFGLCIPADQQY*TGWRFVLNLSQINSRSVERP 559
Query: 454 **TMEM*AAIQSSMCWST*GTS*RKGQ*VKSGD*SWLLA 338
**TM M*AA QSSMCWS *G +*RKGQ* KSGD*S LLA
Sbjct: 560 **TMGM*AATQSSMCWSI*GMN*RKGQ*GKSGD*SLLLA 676
Score = 104 bits (221), Expect = 1e-021
Identities = 50/75 (66%), Positives = 54/75 (72%)
Frame = +2 / -2
Query: 410 AHRRLYCCSHFHSSLRLFC*S*ASLAEV*GRTQACSILLVHQDAQPKIYR*NPLSLPGS* 589
AHRRL CCSH HSS F *S* LA+V + ACSILLV +DAQPKI * PLS P *
Sbjct: 604 AHRRLCCCSHSHSSSGPFY*S*VYLAQVQDKPPACSILLVRRDAQPKIRH*TPLSQPCP* 425
Query: 590 ESCRNLLCGFQSSQA 634
SCR+LLCGFQS QA
Sbjct: 424 VSCRSLLCGFQSFQA 380
>gb|BJ209232.1|BJ209232 BJ209232 Y. Ogihara unpublished cDNA library, Wh Triticum aestivum
cDNA clone wh13d20 5', mRNA sequence
Length = 548
Score = 218 bits (470), Expect = 6e-056
Identities = 87/98 (88%), Positives = 95/98 (96%)
Frame = -2 / +3
Query: 635 LPEKIESRIEGFCRTLMNQVGIKDFNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKA 456
LPEKIESRIEGFCR LM++VGIK+FND+FWAVHPGGPAILNRLE CL+L+P+KLKISRKA
Sbjct: 255 LPEKIESRIEGFCRILMDKVGIKEFNDVFWAVHPGGPAILNRLEVCLQLEPDKLKISRKA 434
Query: 455 LMNYGNVSSNTIFYVLEYLRDELKKGAISEEWGLILAF 342
LMNYGNVSSNT+FYVLEYLRDELKKGAI EEWGLILAF
Sbjct: 435 LMNYGNVSSNTVFYVLEYLRDELKKGAIREEWGLILAF 548
Score = 192 bits (413), Expect = 4e-048
Identities = 73/98 (74%), Positives = 81/98 (82%)
Frame = +3 / -1
Query: 342 KSQDQSPLFTYCPFL*LVPQVLQHIEDCIAAHISIVH*GFSANLELLWLKFKAELKPVQY 521
KSQDQSPLF YCPFL +PQ+LQHIEDC+ AHI IVH G S +LE +WLK K L+PVQY
Sbjct: 548 KSQDQSPLFPYCPFLQFIPQILQHIEDCVTAHIPIVHQGLSTDLEFIWLKLKTNLQPVQY 369
Query: 522 CWSTRMHSPKYIVEILYPYLVHESPAETFYAAFNLLRQ 635
CWST MHSPKYI+E+LYP LVHE PAE FYAAFNL RQ
Sbjct: 368 CWSTGMHSPKYIIELLYPNLVHEYPAEAFYAAFNLFRQ 255
Score = 171 bits (367), Expect = 9e-042
Identities = 77/98 (78%), Positives = 79/98 (80%)
Frame = +1 / -2
Query: 343 KAKINPHSSLIAPFFNSSLKYSST*KIVLLLTFP*FIKAXXXXXXXXXXXXRQNSSLFNI 522
KAKINPHSSLIAPFFNSSLKYSST*K VLLLTFP*FI+A RQ SSLFNI
Sbjct: 547 KAKINPHSSLIAPFFNSSLKYSST*KTVLLLTFP*FIRAFLLILSLSGSS*RQTSSLFNI 368
Query: 523 AGPPGCTAQNISLKSFIPTWFMRVLQKPSMRLSIFSGK 636
AGPPGCTAQN SL SFIPT M +LQKPSMRLSIFSGK
Sbjct: 367 AGPPGCTAQNTSLNSFIPTLSMSILQKPSMRLSIFSGK 254
Score = 144 bits (309), Expect = 9e-034
Identities = 67/98 (68%), Positives = 75/98 (76%)
Frame = -1 / +2
Query: 636 FA*ED*KPHRRFLQDSHEPGRDKGFQRYILGCASWWTSNIEQA*VLP*TSAREAQD*QKS 457
FA*+D*KPHRR LQD+H G DKG Q ILGCAS WTSNIEQA L T AR QD K
Sbjct: 254 FA*KD*KPHRRLLQDTHGQGWDKGVQ*CILGCASRWTSNIEQAGGLSST*AR*TQDQ*KG 433
Query: 456 LNELWKCEQQYNLLCAGVLEGRVKERGNK*RVGIDLGF 343
+ELW+CEQ ++LLCAGV EG ++ERGNK RVGIDLGF
Sbjct: 434 PDELWECEQ*HSLLCAGVFEG*IEERGNKGRVGIDLGF 547
Score = 142 bits (305), Expect = 3e-033
Identities = 70/97 (72%), Positives = 75/97 (77%)
Frame = -3 / +1
Query: 634 CLRRLKAA*KVSAGLS*TR*G*RISTIYFGLCILVDQQY*TGLSSALNFSQRSSRLAEKP 455
CL+RLKAA*K SAG S TR G*R S +YFGLCI VDQQY*TG N SQ +SR E+P
Sbjct: 256 CLKRLKAA*KASAGYSWTRLG*RSSMMYFGLCIPVDQQY*TGWRFVFNLSQINSRSVERP 435
Query: 454 **TMEM*AAIQSSMCWST*GTS*RKGQ*VKSGD*SWL 344
**TM M*A QSSMCWS *G +*RKGQ* KSGD*SWL
Sbjct: 436 **TMGM*AVTQSSMCWSI*GMN*RKGQ*GKSGD*SWL 546
Score = 104 bits (222), Expect = 9e-022
Identities = 50/75 (66%), Positives = 55/75 (73%)
Frame = +2 / -3
Query: 410 AHRRLYCCSHFHSSLRLFC*S*ASLAEV*GRTQACSILLVHQDAQPKIYR*NPLSLPGS* 589
AHRRL CSH HSS F *S* LA+V + ACSILLVH+DAQPKI+ * PLS P *
Sbjct: 480 AHRRLCYCSHSHSSSGPFY*S*VYLAQVEDKPPACSILLVHRDAQPKIHH*TPLSQPCP* 301
Query: 590 ESCRNLLCGFQSSQA 634
SCR+LLCGFQS QA
Sbjct: 300 VSCRSLLCGFQSFQA 256
>gb|CA502438.1|CA502438 WHE4047_E05_J09ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE4047_E05_J09, mRNA sequence
Length = 634
Score = 82.2 bits (173), Expect(2) = 4e-024
Identities = 35/67 (52%), Positives = 48/67 (71%)
Frame = -2 / +2
Query: 563 FNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYLRDELK 384
++ +FWAVHPGGPAIL ++E L L KL+ SR AL ++GN SSNTI YVLE + +E +
Sbjct: 158 YDKMFWAVHPGGPAILTKMEGRLGLDGGKLRASRSALRDFGNASSNTIVYVLENMVEESR 337
Query: 383 KGAISEE 363
+ A + E
Sbjct: 338 QRAEAPE 358
Score = 49.6 bits (102), Expect(2) = 4e-024
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / +2
Query: 365 EWGLILAFGPGITFEGLLVRGVK 297
EWGLILAFGPGITFEG+L R ++
Sbjct: 395 EWGLILAFGPGITFEGILARNLQ 463
>gb|CA594953.1|CA594953 wpa1c.pk006.e16 wpa1c Triticum aestivum cDNA clone wpa1c.pk006.e16
5' end, mRNA sequence
Length = 532
Score = 82.2 bits (173), Expect(2) = 4e-024
Identities = 35/67 (52%), Positives = 48/67 (71%)
Frame = -2 / +3
Query: 563 FNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYLRDELK 384
++ +FWAVHPGGPAIL ++E L L KL+ SR AL ++GN SSNTI YVLE + +E +
Sbjct: 117 YDKMFWAVHPGGPAILTKMEGRLGLDGGKLRASRSALRDFGNASSNTIVYVLENMVEESR 296
Query: 383 KGAISEE 363
+ A + E
Sbjct: 297 QRAEAPE 317
Score = 49.6 bits (102), Expect(2) = 4e-024
Identities = 18/23 (78%), Positives = 21/23 (91%)
Frame = -2 / +3
Query: 365 EWGLILAFGPGITFEGLLVRGVK 297
EWGLILAFGPGITFEG+L R ++
Sbjct: 354 EWGLILAFGPGITFEGILARNLQ 422
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 598,983,436
Number of Sequences: 636343
Number of extensions: 8344542
Number of successful extensions: 77730
Number of sequences better than 1.0e-020: 9
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 14012582972
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)