TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3106160.2.1
(788 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD927723.1|CD927723 GR45.102P17F010322 GR45 Triticum aes... 214 8e-056
gb|CD893289.1|CD893289 G118.123F12F010829 G118 Triticum aes... 214 8e-056
gb|CA680933.1|CA680933 wlm24.pk0013.b3 wlm24 Triticum aesti... 207 2e-052
gb|CD924532.1|CD924532 G750.113G09F010706 G750 Triticum aes... 189 1e-048
gb|DQ157841.1| Triticum turgidum strain Langdon chromosome ... 105 7e-042
gb|BQ901513.1|BQ901513 Ta02_16e04_R Ta02_AAFC_ECORC_Fusariu... 140 2e-032
gb|CD869974.1|CD869974 AZO2.113C08F001128 AZO2 Triticum aes... 136 4e-032
gb|CD454178.1|CD454178 WHE0987-0990_M05_M05ZT CS wheat pre-... 112 5e-025
>gb|CD927723.1|CD927723 GR45.102P17F010322 GR45 Triticum aestivum cDNA clone GR45102P17,
mRNA sequence
Length = 695
Score = 214 bits (461), Expect(2) = 8e-056
Identities = 86/132 (65%), Positives = 102/132 (77%)
Frame = +3 / +3
Query: 3 AAVLHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRAAFIIASTASEEEMLRWYNE 182
AA+LHYTYTKFSDLTSRRDRCGCKPTKEDVKRCF+L+FDR AFIIASTA+E+EM WY E
Sbjct: 21 AAILHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFILEFDRLAFIIASTATEQEMRNWYRE 200
Query: 183 RVVWTDKQLNLKLLRKGVLTRIYTPMTIVQSLRESGVFTSAIAAGQPAVNAKLSPKETNA 362
VVWTDK NLKLLRKGVLTRIY PM I++ L+ESG+F A+ + + A + + +
Sbjct: 201 HVVWTDKDTNLKLLRKGVLTRIYAPMAIIRGLKESGIFIDAVTSAKAHPKANIGLENNES 380
Query: 363 QSQNVTAPGNMT 398
NVTA + T
Sbjct: 381 IHTNVTAAQSTT 416
Score = 138 bits (296), Expect(2) = 1e-032
Identities = 67/101 (66%), Positives = 73/101 (72%)
Frame = -2 / -2
Query: 304 AEVKTPDSRRL*TIVIGVYMRVSTPFLRSFKFSCLSVHTTRSLYQRSISSSLAVDAIIKA 125
A +K PDS R I IG YMRV TPFL+SFKF LSVHTT S YQ IS S+AVDAI+KA
Sbjct: 322 ASMKMPDSLRPRIIAIGAYMRVKTPFLKSFKFVSLSVHTTCSRYQFLISCSVAVDAIMKA 143
Query: 124 ALSKSNMKHLLTSSFVGLHPQRSRLDVKSENLVYV*CRTAA 2
LS S +KH TSSFVGL P S L+VKSEN V V*CR AA
Sbjct: 142 NLSNSKIKHRFTSSFVGLQPHLSLLEVKSENFV*V*CRIAA 20
Score = 24.9 bits (48), Expect(2) = 1e-032
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -2 / -2
Query: 526 LCSSKLGGDNGGTAVTLVSLKAR 458
+ S K GGD GGTA + S+ +R
Sbjct: 532 MSSRKPGGDIGGTASSWTSIISR 464
Score = 24.4 bits (47), Expect(2) = 8e-056
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = +3 / +3
Query: 459 LAFSDTNVTAVPPLSPPSLDE 521
L D AVPP+SPP E
Sbjct: 465 LEMIDVQEEAVPPMSPPGFLE 527
>gb|CD893289.1|CD893289 G118.123F12F010829 G118 Triticum aestivum cDNA clone G118123F12,
mRNA sequence
Length = 689
Score = 214 bits (461), Expect(2) = 8e-056
Identities = 86/132 (65%), Positives = 102/132 (77%)
Frame = +3 / +3
Query: 3 AAVLHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRAAFIIASTASEEEMLRWYNE 182
AA+LHYTYTKFSDLTSRRDRCGCKPTKEDVKRCF+L+FDR AFIIASTA+E+EM WY E
Sbjct: 111 AAILHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFILEFDRLAFIIASTATEQEMRNWYRE 290
Query: 183 RVVWTDKQLNLKLLRKGVLTRIYTPMTIVQSLRESGVFTSAIAAGQPAVNAKLSPKETNA 362
VVWTDK NLKLLRKGVLTRIY PM I++ L+ESG+F A+ + + A + + +
Sbjct: 291 HVVWTDKDTNLKLLRKGVLTRIYAPMAIIRGLKESGIFIDAVTSAKAHPKANIGLENNES 470
Query: 363 QSQNVTAPGNMT 398
NVTA + T
Sbjct: 471 IHTNVTAAQSTT 506
Score = 138 bits (296), Expect(2) = 1e-032
Identities = 67/101 (66%), Positives = 73/101 (72%)
Frame = -2 / -2
Query: 304 AEVKTPDSRRL*TIVIGVYMRVSTPFLRSFKFSCLSVHTTRSLYQRSISSSLAVDAIIKA 125
A +K PDS R I IG YMRV TPFL+SFKF LSVHTT S YQ IS S+AVDAI+KA
Sbjct: 412 ASMKMPDSLRPRIIAIGAYMRVKTPFLKSFKFVSLSVHTTCSRYQFLISCSVAVDAIMKA 233
Query: 124 ALSKSNMKHLLTSSFVGLHPQRSRLDVKSENLVYV*CRTAA 2
LS S +KH TSSFVGL P S L+VKSEN V V*CR AA
Sbjct: 232 NLSNSKIKHRFTSSFVGLQPHLSLLEVKSENFV*V*CRIAA 110
Score = 24.9 bits (48), Expect(2) = 1e-032
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -2 / -2
Query: 526 LCSSKLGGDNGGTAVTLVSLKAR 458
+ S K GGD GGTA + S+ +R
Sbjct: 622 MSSRKPGGDIGGTASSWTSIISR 554
Score = 24.4 bits (47), Expect(2) = 8e-056
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = +3 / +3
Query: 459 LAFSDTNVTAVPPLSPPSLDE 521
L D AVPP+SPP E
Sbjct: 555 LEMIDVQEEAVPPMSPPGFLE 617
>gb|CA680933.1|CA680933 wlm24.pk0013.b3 wlm24 Triticum aestivum cDNA clone wlm24.pk0013.b3
5' end, mRNA sequence
Length = 654
Score = 207 bits (446), Expect = 2e-052
Identities = 81/113 (71%), Positives = 94/113 (83%)
Frame = +3 / +3
Query: 3 AAVLHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRAAFIIASTASEEEMLRWYNE 182
AA+LHYTYTKFSDLTSRRDRCGCKPTKEDVKRCF+L+FDR AFIIASTA+E+EM WY E
Sbjct: 39 AAILHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFILEFDRLAFIIASTATEQEMRNWYRE 218
Query: 183 RVVWTDKQLNLKLLRKGVLTRIYTPMTIVQSLRESGVFTSAIAAGQPAVNAKL 341
VVWTDK NLKLLRKGVLTRIY PM I++ L+ESG+F A+ + + A +
Sbjct: 219 HVVWTDKDTNLKLLRKGVLTRIYAPMAIIRGLKESGIFIDAVTSAKAHPKANI 377
Score = 138 bits (296), Expect = 7e-032
Identities = 67/101 (66%), Positives = 73/101 (72%)
Frame = -2 / -3
Query: 304 AEVKTPDSRRL*TIVIGVYMRVSTPFLRSFKFSCLSVHTTRSLYQRSISSSLAVDAIIKA 125
A +K PDS R I IG YMRV TPFL+SFKF LSVHTT S YQ IS S+AVDAI+KA
Sbjct: 340 ASMKMPDSLRPRIIAIGAYMRVKTPFLKSFKFVSLSVHTTCSRYQFLISCSVAVDAIMKA 161
Query: 124 ALSKSNMKHLLTSSFVGLHPQRSRLDVKSENLVYV*CRTAA 2
LS S +KH TSSFVGL P S L+VKSEN V V*CR AA
Sbjct: 160 NLSNSKIKHRFTSSFVGLQPHLSLLEVKSENFV*V*CRIAA 38
>gb|CD924532.1|CD924532 G750.113G09F010706 G750 Triticum aestivum cDNA clone G750113G09,
mRNA sequence
Length = 661
Score = 189 bits (408), Expect(2) = 1e-048
Identities = 77/123 (62%), Positives = 92/123 (74%)
Frame = +3 / +1
Query: 30 KFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRAAFIIASTASEEEMLRWYNERVVWTDKQL 209
KFSDLTSRRDRCGCKPTKEDVKRCF+L+FD AFIIASTA+E+EM WY E VVWTDK
Sbjct: 13 KFSDLTSRRDRCGCKPTKEDVKRCFILEFDXMAFIIASTATEQEMRNWYREHVVWTDKDT 192
Query: 210 NLKLLRKGVLTRIYTPMTIVQSLRESGVFTSAIAAGQPAVNAKLSPKETNAQSQNVTAPG 389
NLKLLRKGVLTRIY PM I++ L+ESG+F A+ + + A + + + NVTA
Sbjct: 193 NLKLLRKGVLTRIYAPMAIIRGLKESGIFIDAVTSAKAHPKANMGLENNESIHTNVTAAQ 372
Query: 390 NMT 398
+ T
Sbjct: 373 STT 381
Score = 124 bits (266), Expect(2) = 1e-028
Identities = 59/91 (64%), Positives = 65/91 (71%)
Frame = -2 / -3
Query: 304 AEVKTPDSRRL*TIVIGVYMRVSTPFLRSFKFSCLSVHTTRSLYQRSISSSLAVDAIIKA 125
A +K PDS R I IG YMRV TPFL+SFKF LSVHTT S YQ IS S+AVDAI+KA
Sbjct: 287 ASMKMPDSLRPRIIAIGAYMRVKTPFLKSFKFVSLSVHTTCSRYQFLISCSVAVDAIMKA 108
Query: 124 ALSKSNMKHLLTSSFVGLHPQRSRLDVKSEN 32
S S +KH TSSFVGL P S L+VKSEN
Sbjct: 107 IXSNSKIKHRFTSSFVGLQPHLSLLEVKSEN 15
Score = 24.9 bits (48), Expect(2) = 1e-048
Identities = 10/21 (47%), Positives = 11/21 (52%)
Frame = +3 / +1
Query: 459 LAFSDTNVTAVPPLSPPSLDE 521
L D AVPP+SPP E
Sbjct: 430 LEMMDVQEEAVPPMSPPGFLE 492
Score = 24.9 bits (48), Expect(2) = 1e-028
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -2 / -3
Query: 526 LCSSKLGGDNGGTAVTLVSLKAR 458
+ S K GGD GGTA + S+ +R
Sbjct: 497 MSSRKPGGDIGGTASSWTSIISR 429
>gb|DQ157841.1| Triticum turgidum strain Langdon chromosome 1 clone BAC 1219A5 map 1BS,
WORKING DRAFT SEQUENCE, 16 unordered pieces
Length = 105039
Score = 105 bits (225), Expect(5) = 7e-042
Identities = 41/41 (100%), Positives = 41/41 (100%)
Frame = +3 / -2
Query: 3 AAVLHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRA 125
AAVLHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRA
Sbjct: 49199 AAVLHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRA 49077
Score = 98.7 bits (209), Expect(3) = 5e-029
Identities = 45/57 (78%), Positives = 48/57 (84%)
Frame = -2 / +1
Query: 172 QRSISSSLAVDAIIKAALSKSNMKHLLTSSFVGLHPQRSRLDVKSENLVYV*CRTAA 2
Q+S SS ++ ALSKSNMKHLLTSSFVGLHPQRSRLDVKSENLVYV*CRTAA
Sbjct: 49030 QKSGSSLQENRVALRTALSKSNMKHLLTSSFVGLHPQRSRLDVKSENLVYV*CRTAA 49200
Score = 69.3 bits (145), Expect(5) = 7e-042
Identities = 28/34 (82%), Positives = 29/34 (85%)
Frame = +3 / -1
Query: 174 YNERVVWTDKQLNLKLLRKGVLTRIYTPMTIVQS 275
YNERVVW DKQLNLKLLRKGVLTRIY PM + S
Sbjct: 48606 YNERVVWNDKQLNLKLLRKGVLTRIYAPMVLNNS 48505
Score = 57.0 bits (118), Expect(3) = 5e-029
Identities = 23/29 (79%), Positives = 25/29 (86%)
Frame = -2 / +2
Query: 259 IGVYMRVSTPFLRSFKFSCLSVHTTRSLY 173
IG MRV+TPFLRSF+ SCLS HTTRSLY
Sbjct: 48521 IGA*MRVNTPFLRSFRLSCLSFHTTRSLY 48607
Score = 39.1 bits (79), Expect(5) = 7e-042
Identities = 16/20 (80%), Positives = 17/20 (85%)
Frame = +3 / -3
Query: 114 FDRAAFIIASTASEEEMLRW 173
F AFIIASTASEE+MLRW
Sbjct: 48892 FTMQAFIIASTASEEDMLRW 48833
Score = 32.2 bits (64), Expect(5) = 7e-042
Identities = 15/26 (57%), Positives = 16/26 (61%)
Frame = +3 / -1
Query: 264 IVQSLRESGVFTSAIAAGQPAVNAKL 341
IVQ LRESGVFTS I A +L
Sbjct: 48426 IVQGLRESGVFTSVIRAAVDNAKVRL 48349
Score = 30.4 bits (60), Expect(5) = 7e-042
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +3 / -1
Query: 486 AVPPLSPPSLDEHR 527
AVPP+SPP LDE R
Sbjct: 48213 AVPPMSPPGLDEAR 48172
Score = 28.6 bits (56), Expect(3) = 5e-029
Identities = 13/17 (76%), Positives = 13/17 (76%)
Frame = -2 / +2
Query: 313 AAMAEVKTPDSRRL*TI 263
A M EVKTPDSRR TI
Sbjct: 48377 ALMTEVKTPDSRRPCTI 48427
>gb|BQ901513.1|BQ901513 Ta02_16e04_R
Ta02_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_he
ads Triticum aestivum cDNA clone Ta02_16e04, mRNA
sequence
Length = 461
Score = 140 bits (301), Expect = 2e-032
Identities = 56/57 (98%), Positives = 57/57 (100%)
Frame = +3 / +2
Query: 3 AAVLHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRAAFIIASTASEEEMLRW 173
AAVLHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRAAFIIASTASEE+MLRW
Sbjct: 290 AAVLHYTYTKFSDLTSRRDRCGCKPTKEDVKRCFMLDFDRAAFIIASTASEEDMLRW 460
Score = 123 bits (264), Expect = 2e-027
Identities = 56/57 (98%), Positives = 56/57 (98%)
Frame = -2 / -3
Query: 172 QRSISSSLAVDAIIKAALSKSNMKHLLTSSFVGLHPQRSRLDVKSENLVYV*CRTAA 2
QRSISSSLAVDAIIKAALSKSNMKHLLTSSFVGLHPQRSRLDVKSENLVYV*C TAA
Sbjct: 459 QRSISSSLAVDAIIKAALSKSNMKHLLTSSFVGLHPQRSRLDVKSENLVYV*CSTAA 289
Score = 103 bits (219), Expect = 3e-021
Identities = 44/57 (77%), Positives = 47/57 (82%)
Frame = -1 / -2
Query: 173 PAKHFFFTSS*CNYKSCSVKI*HETSLNIFFCRLASTAITSRCQI*KFSVCIMQNCS 3
PAKH FTSS*CNYKSCSVKI HETS NIF LASTAITS C +*KF VCI+Q+CS
Sbjct: 460 PAKHILFTSS*CNYKSCSVKIQHETSFNIFLRGLASTAITSGC*V*KFGVCIVQHCS 290
Score = 102 bits (218), Expect = 4e-021
Identities = 45/57 (78%), Positives = 50/57 (87%)
Frame = +2 / +1
Query: 2 GCSSALYIH*IFRFDI*T*SLWMQAYKRRC*EMFHVRF*QSSFYNCINC**RRNASL 172
GCS+ALYIH IFR +I T*SLWMQA++ RC*+MFHV F*QSSFYNCINC**R ASL
Sbjct: 289 GCSAALYIHQIFRLNIQT*SLWMQAHEGRC*KMFHVGF*QSSFYNCINC**RGYASL 459
>gb|CD869974.1|CD869974 AZO2.113C08F001128 AZO2 Triticum aestivum cDNA clone AZO2113C08,
mRNA sequence
Length = 724
Score = 136 bits (291), Expect(2) = 4e-032
Identities = 56/102 (54%), Positives = 70/102 (68%)
Frame = +3 / +3
Query: 93 KRCFMLDFDRAAFIIASTASEEEMLRWYNERVVWTDKQLNLKLLRKGVLTRIYTPMTIVQ 272
KRCF+L+FDR AFIIASTA+E+EM WY E VVWTDK NLKLLRKGVLTRIY PM I++
Sbjct: 3 KRCFILEFDRLAFIIASTATEQEMRNWYREHVVWTDKDTNLKLLRKGVLTRIYAPMAIIR 182
Query: 273 SLRESGVFTSAIAAGQPAVNAKLSPKETNAQSQNVTAPGNMT 398
L+ESG+F A+ + + A + + + VTA T
Sbjct: 183 GLKESGIFIDAVTSAKAHPKANIGLENNESIHTKVTASQTTT 308
Score = 23.1 bits (44), Expect(2) = 4e-032
Identities = 9/21 (42%), Positives = 11/21 (52%)
Frame = +3 / +3
Query: 459 LAFSDTNVTAVPPLSPPSLDE 521
L D A+PP+SPP E
Sbjct: 357 LEMIDVQEEAMPPMSPPGFLE 419
>gb|CD454178.1|CD454178 WHE0987-0990_M05_M05ZT CS wheat pre-anthesis spike cDNA library
Triticum aestivum cDNA clone WHE0987-0990_M05_M05, mRNA
sequence
Length = 712
Score = 112 bits (240), Expect(2) = 5e-025
Identities = 47/89 (52%), Positives = 60/89 (67%)
Frame = +3 / -3
Query: 132 IIASTASEEEMLRWYNERVVWTDKQLNLKLLRKGVLTRIYTPMTIVQSLRESGVFTSAIA 311
IIASTA+E+EM WY E VVWTDK NLKLLRKGVLTRIY PM I++ L+ESG+F A+
Sbjct: 710 IIASTATEQEMRNWYREHVVWTDKDTNLKLLRKGVLTRIYAPMAIIRGLKESGIFIDAVT 531
Query: 312 AGQPAVNAKLSPKETNAQSQNVTAPGNMT 398
+ + A + + + NVTA + T
Sbjct: 530 SAKAHPKANIGLENNESIHTNVTAAQSTT 444
Score = 22.6 bits (43), Expect(2) = 5e-025
Identities = 9/21 (42%), Positives = 10/21 (47%)
Frame = +3 / -3
Query: 459 LAFSDTNVTAVPPLSPPSLDE 521
L D VPP+SPP E
Sbjct: 395 LEMIDVQEEVVPPMSPPGFLE 333
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 730,224,028
Number of Sequences: 636343
Number of extensions: 10556808
Number of successful extensions: 78844
Number of sequences better than 1.0e-020: 13
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 18535631906
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)