TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067516.2.1
(605 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ300151.1|BJ300151 BJ300151 Y. Ogihara unpublished cDNA... 180 2e-060
gb|CZ889026.1|CZ889026 ftaa002d005b06t0 Bread wheat methyla... 135 2e-031
gb|BJ283383.1|BJ283383 BJ283383 Y. Ogihara unpublished cDNA... 85 2e-024
gb|BQ240367.1|BQ240367 TaE05018E03R TaE05 Triticum aestivum... 85 8e-024
>gb|BJ300151.1|BJ300151 BJ300151 Y. Ogihara unpublished cDNA library, Wh_SL Triticum
aestivum cDNA clone whsl17l22 3', mRNA sequence
Length = 694
Score = 180 bits (388), Expect(4) = 2e-060
Identities = 75/86 (87%), Positives = 81/86 (94%)
Frame = -2 / -2
Query: 604 GNASIEERLKSIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDVSIEAIINKVTK 425
GN S+EE L+SIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDVS+ AIINKVT+
Sbjct: 672 GNVSVEEILRSIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDVSVSAIINKVTQ 493
Query: 424 LRRDIIAGQEDKDFIEENSWKYSLLD 347
LRRDII+G EDKDFIEENSWKY LL+
Sbjct: 492 LRRDIISGSEDKDFIEENSWKYELLE 415
Score = 152 bits (326), Expect(4) = 5e-054
Identities = 70/86 (81%), Positives = 74/86 (86%)
Frame = +3 / +2
Query: 348 SNRLYFQLFSSIKSLSSWPAMISLLNFVTLLMIASMETSNASFNVSSFDLGSAYITFGIR 527
SN YFQLFSSIKSLSS P MISLLN VTLLMIA ETSNASF+VSSFDLGSAYITFG+R
Sbjct: 416 SNSSYFQLFSSIKSLSSLPEMISLLNCVTLLMIAETETSNASFSVSSFDLGSAYITFGMR 595
Query: 528 LMTSSLICLTTSG*ILFSLSSMLAFP 605
+TSSLICLTTSG IL S+SS L FP
Sbjct: 596 FITSSLICLTTSGWILLSISSTLTFP 673
Score = 107 bits (229), Expect(4) = 2e-037
Identities = 48/85 (56%), Positives = 56/85 (65%)
Frame = +2 / +1
Query: 350 *QTILPAILFDKIFVFLACDDISSQLCHFVNDRLYGNIKCIFQCLQFRSWIGIYHLWYQI 529
*Q ILPAILF+KIF+F A DDISSQLCHFV+D NIK + QFR WI IYHLWY+I
Sbjct: 418 *QFILPAILFNKIFIFAARDDISSQLCHFVDDCRNRNIKRVL*RFQFRPWISIYHLWYEI 597
Query: 530 XXXXXXXXXXIWVNTL*PLLNAGIS 604
+W++T LLN IS
Sbjct: 598 YNLFSHLLDNVWMDTSQYLLNTDIS 672
Score = 97.3 bits (206), Expect(4) = 2e-031
Identities = 53/85 (62%), Positives = 60/85 (70%)
Frame = -3 / -3
Query: 603 EMPALRRG*RVFTQMWSSK*ERRSSI*YQR*YMPIQDRNWRH*KMHLMFP*RRSLTK*QS 424
EM LRR * V Q SS+*E+R I YQR*YM IQ RNW+ *+ LMF R+S TK* +
Sbjct: 671 EMSVLRRY*EVSIQTLSSR*EKRL*ISYQR*YMLIQGRNWKR*RTRLMFRFRQSSTK*HN 492
Query: 423 *EEISSQAKKTKILSKRIAGSIVC* 349
*EEISS A K KIL KRIAGS+ C*
Sbjct: 491 *EEISSLAAKIKILLKRIAGSMNC* 417
Score = 45.1 bits (92), Expect(4) = 2e-060
Identities = 20/33 (60%), Positives = 23/33 (69%)
Frame = -3 / -3
Query: 303 SRSLKIKVEILVVHLQKLPRILGKVNREDRIEP 205
S S K +V+ILV+H K PRILGK N E RI P
Sbjct: 368 SPSPKTRVQILVIHQLKRPRILGKSNEETRIRP 270
Score = 42.3 bits (86), Expect(4) = 5e-054
Identities = 19/23 (82%), Positives = 20/23 (86%)
Frame = +3 / +2
Query: 228 SLFQEFLAASADEPLESPPLSLG 296
S+FQEFLAASADE ES PLSLG
Sbjct: 293 SIFQEFLAASADELPESAPLSLG 361
Score = 41.8 bits (85), Expect(4) = 5e-054
Identities = 16/26 (61%), Positives = 19/26 (73%)
Frame = +3 / +2
Query: 285 LSLGFEKNGSHL*GPIACCPSSNRLY 362
L LGFEKNGSH *GP CPS++ +
Sbjct: 356 LGLGFEKNGSHS*GPTVRCPSNSSYF 433
Score = 39.6 bits (80), Expect(4) = 2e-037
Identities = 21/41 (51%), Positives = 24/41 (58%)
Frame = +2 / +1
Query: 176 LISSQRRPTHGSILSSLFTFPRILGSFCR*TTRISTFIFRL 298
L+ S+ G IL S F FPRILG F * TRI T +F L
Sbjct: 241 LLISKSSQLCGLILVSSFDFPRILGRFS**ITRICTLVFGL 363
Score = 37.3 bits (75), Expect(4) = 2e-060
Identities = 10/15 (66%), Positives = 14/15 (93%)
Frame = -2 / -2
Query: 346 DGQQAIGPHKWDPFF 302
+GQ+ +GPH+WDPFF
Sbjct: 417 EGQRTVGPHEWDPFF 373
Score = 36.8 bits (74), Expect(4) = 2e-031
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = -3 / -3
Query: 342 DSRQLDLTNGIHSSRSLKIKVEILVVHLQKLPR 244
DS QLDL +GIHSSRS K + ++ + +L R
Sbjct: 413 DSGQLDLMSGIHSSRSPSPKTRVQILVIHQLKR 315
Score = 35.4 bits (71), Expect(4) = 2e-037
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +2 / +1
Query: 296 LREEWIPFVRSNCLLS 343
LREEWIP +RSNC LS
Sbjct: 367 LREEWIPLMRSNCPLS 414
Score = 34.5 bits (69), Expect(4) = 2e-031
Identities = 16/30 (53%), Positives = 21/30 (70%)
Frame = -2 / -2
Query: 259 AEAAKNSWKSEQRGQD*TMSWSSLG*DQGS 170
AEAAKNSWK E+ Q+*T ++L +Q S
Sbjct: 324 AEAAKNSWKIERGDQN*TAQLTALRDEQNS 235
Score = 32.2 bits (64), Expect(4) = 5e-054
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +2 / +3
Query: 23 FPKHFGGMASYNCRVT*IINDVHDHHNYWI 112
F + FGG SY IIN++HDH Y I
Sbjct: 96 FSRTFGGNISYTGCRGWIINNIHDHQGYSI 185
Score = 29.0 bits (57), Expect(4) = 2e-037
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +1 / +2
Query: 22 FSKAFWWNGFIQLQGDIDHQ*CS*SP 99
F K FWW F+ +DHQ +*SP
Sbjct: 95 FLKDFWW*HFLHRLQGLDHQQHT*SP 172
Score = 27.2 bits (53), Expect(4) = 2e-060
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = -1 / -2
Query: 113 RSSSYGDHEHH**SMSPCSCMKPFHQNALEK 21
R S GDH **S C K +HQ +L K
Sbjct: 186 RCCSLGDHVCC**SSPCSRCKKCYHQKSLRK 94
Score = 23.1 bits (44), Expect(4) = 2e-031
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2 / -3
Query: 112 DPVVMVIMNIIDDLCHPAVV*SHSTK 35
D V +VIM ++DD A V + +TK
Sbjct: 185 DAVALVIMYVVDDPAPAAGVRNVTTK 108
>gb|CZ889026.1|CZ889026 ftaa002d005b06t0 Bread wheat methylation filtered library (LibID:
111) Triticum aestivum genomic, DNA sequence
Length = 531
Score = 135 bits (289), Expect = 5e-031
Identities = 54/85 (63%), Positives = 64/85 (75%)
Frame = -2 / +2
Query: 463 DVSIEAIINKVTKLRRDIIAGQEDKDFIEENSWKYSLLDDGQQAIGPHKWDPFFXXXXXX 284
DVS+E IINKV++LRRDII EDKDF+EENSWKY LL++GQ+ IGPH+WDPFF
Sbjct: 2 DVSVEVIINKVSQLRRDIIEDHEDKDFVEENSWKYDLLEEGQRTIGPHEWDPFFSKPKDK 181
Query: 283 XXXXXXXSAEAAKNSWKSEQRGQD* 209
S AAKNSWK+EQRGQ+*
Sbjct: 182 GGDATNPSTNAAKNSWKNEQRGQN* 256
Score = 133 bits (284), Expect(2) = 2e-031
Identities = 61/82 (74%), Positives = 64/82 (78%)
Frame = +3 / -1
Query: 219 PLCSLFQEFLAASADEPLESPPLSLGFEKNGSHL*GPIACCPSSNRLYFQLFSSIKSLSS 398
PLCS FQEFLAA D + SPPLSLG EKNGSH *GPI CPSSNR YFQLFSS KSLSS
Sbjct: 246 PLCSFFQEFLAALVDGLVASPPLSLGLEKNGSHS*GPIVLCPSSNRSYFQLFSSTKSLSS 67
Query: 399 WPAMISLLNFVTLLMIASMETS 464
+MISLLN TLL+I S ETS
Sbjct: 66 *SSMISLLNCDTLLIITSTETS 1
Score = 109 bits (232), Expect = 4e-023
Identities = 49/84 (58%), Positives = 59/84 (70%)
Frame = +2 / -3
Query: 212 ILSSLFTFPRILGSFCR*TTRISTFIFRLREEWIPFVRSNCLLSIV*QTILPAILFDKIF 391
+L+SLF FP +LGS R + IST + LREE +PF+R NC L Q ILPAILF+KIF
Sbjct: 253 VLTSLFVFPGVLGSISRWISSISTLVLGLREERVPFMRPNCPLPFFQQIILPAILFNKIF 74
Query: 392 VFLACDDISSQLCHFVNDRLYGNI 463
+F+ DDISSQL H VN LY NI
Sbjct: 73 IFMIFDDISSQL*HLVNYYLYRNI 2
Score = 23.1 bits (44), Expect(2) = 2e-031
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = +2 / -2
Query: 71 *IINDVHDHHNYWI 112
*II ++HDH +Y +
Sbjct: 395 *IIKNIHDHQSYCV 354
>gb|BJ283383.1|BJ283383 BJ283383 Y. Ogihara unpublished cDNA library, Wh_r Triticum
aestivum cDNA clone whr15l16 3', mRNA sequence
Length = 609
Score = 85.4 bits (180), Expect(2) = 2e-024
Identities = 37/75 (49%), Positives = 52/75 (69%)
Frame = -2 / -1
Query: 601 NASIEERLKSIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDVSIEAIINKVTKL 422
NASIEE L+ I P V +MREEVI LIP V+Y +P +K KDAFDV++E ++++V K
Sbjct: 453 NASIEEVLRKIPPAKVARMREEVIRLIPAVMYREPAAKGVQFKDAFDVAVERVVDRVAKR 274
Query: 421 RRDIIAGQEDKDFIE 377
RR G+E +D ++
Sbjct: 273 RRAAAEGRECRDSVD 229
Score = 47.3 bits (97), Expect(2) = 2e-024
Identities = 15/23 (65%), Positives = 21/23 (91%)
Frame = -2 / -1
Query: 373 NSWKYSLLDDGQQAIGPHKWDPF 305
+SWKY+LL+DGQ IGPH++DP+
Sbjct: 222 DSWKYNLLEDGQTEIGPHEFDPY 154
>gb|BQ240367.1|BQ240367 TaE05018E03R TaE05 Triticum aestivum cDNA clone TaE05018E03R, mRNA
sequence
Length = 398
Score = 84.9 bits (179), Expect(2) = 8e-024
Identities = 37/74 (50%), Positives = 51/74 (68%)
Frame = -2 / +1
Query: 601 NASIEERLKSIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDVSIEAIINKVTKL 422
NASIEE L+ I P V +MREEV+ LIP V+Y +P +K KDAFDV++E +I++V K
Sbjct: 97 NASIEEVLRKIPPAKVARMREEVVRLIPSVMYREPAAKGVEFKDAFDVAVERVIDRVAKR 276
Query: 421 RRDIIAGQEDKDFI 380
RR G+E +D +
Sbjct: 277 RRAAAEGRECRDSV 318
Score = 46.0 bits (94), Expect(2) = 8e-024
Identities = 15/23 (65%), Positives = 19/23 (82%)
Frame = -2 / +1
Query: 373 NSWKYSLLDDGQQAIGPHKWDPF 305
+SWKY LL+DG IGPH++DPF
Sbjct: 328 DSWKYDLLEDGHTEIGPHEFDPF 396
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 516,827,841
Number of Sequences: 636343
Number of extensions: 6350098
Number of successful extensions: 56307
Number of sequences better than 1.0e-020: 5
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 13125710632
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)