TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3023663.2.1
(613 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA500452.1|CA500452 WHE4019_G05_N09ZT Wheat meiotic anth... 207 8e-053
>gb|CA500452.1|CA500452 WHE4019_G05_N09ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE4019_G05_N09, mRNA sequence
Length = 735
Score = 207 bits (447), Expect = 8e-053
Identities = 87/122 (71%), Positives = 96/122 (78%)
Frame = -2 / +3
Query: 594 PETNRKDVNLPGILQSPISNMLRKVEKVSQDIPKHRKVTHPEYEVETANGRITKRRKTRS 415
PET RKDVN+ G++ SPISN++RKVEK SQD+ HRKVTH EYEVETANG+ITKRRKT+S
Sbjct: 351 PETKRKDVNISGVIHSPISNVMRKVEKASQDVTNHRKVTHHEYEVETANGKITKRRKTKS 530
Query: 414 PVMFGEPNSQKPLHNXXXXXXXXXXXVPTRSRAHPANIGELFSEGSLNPYADDPYAFD*K 235
VMFGEPN+QK L N V SR HPANIGELFSEGSLNPYADDPYAF *K
Sbjct: 531 TVMFGEPNTQKSLQNTADKDVTKTRKVVAGSRPHPANIGELFSEGSLNPYADDPYAFG*K 710
Query: 234 LT 229
LT
Sbjct: 711 LT 716
Score = 187 bits (402), Expect = 1e-046
Identities = 89/122 (72%), Positives = 92/122 (75%)
Frame = +2 / -3
Query: 230 VSF*SKA*GSSAYGFKEPSENNSPMFAGCARDLVGTFFSFLTSLSVVLCNGF*EFGSPNM 409
VSF* A*GSSAYGFKEPSENNSP+ AG RD TF F+TSLS V CN F* FGSPNM
Sbjct: 715 VSF*PNA*GSSAYGFKEPSENNSPILAGWGRDPATTFLVFVTSLSAVFCNDF*VFGSPNM 536
Query: 410 TGLLVFLRFVILPFAVSTSYSGWVTFLCLGIS*DTFSTFLNMFEIGDCKIPGKFTSFRLV 589
T LLVFLRFVI PFAVSTSYS VTFL L S* FSTFL EIG+C P FTSFRLV
Sbjct: 535 TVLLVFLRFVIFPFAVSTSYSWCVTFL*LVTS*LAFSTFLITLEIGECITPDMFTSFRLV 356
Query: 590 SG 595
SG
Sbjct: 355 SG 350
Score = 181 bits (390), Expect = 6e-045
Identities = 82/134 (61%), Positives = 97/134 (72%)
Frame = +1 / -2
Query: 211 KNDTSRRELLVKSIRVVSIRIQGTLRE*FTNVCRMCTGSCRNLF*FFNIFVSSIMQWFLR 390
K +RRELL K IRV+SI IQG LR+*FT++ RM TGSC NL F +I VSSI+Q L
Sbjct: 734 KISINRRELLTKCIRVISIWIQGALRK*FTDIGRMGTGSCNNLSCFCDILVSSILQ*LLS 555
Query: 391 VRFTKHDRAPSFPALCDSSICCLNFVFRVGYLSMFRNILRHFLYLSQHVRNW*L*NPR*V 570
+RF KHD A SFPALCD SICCL+F+F V YLS+ NIL FLYL +VRNW + N R V
Sbjct: 554 IRFPKHDSALSFPALCDFSICCLHFIFMVCYLSVISNILTCFLYLPHYVRNWRVYNSRYV 375
Query: 571 YVLPIGLRTYQLDL 612
Y+LP GLRT+ L
Sbjct: 374 YILPFGLRTWTWQL 333
Score = 166 bits (358), Expect = 1e-040
Identities = 78/125 (62%), Positives = 89/125 (71%)
Frame = -1 / +2
Query: 598 KSGDQSEGRKLTWDFTITNFEHVEKGRESVSGYS*T*KGNPP*IRS*DSKWKNHKAQEN* 419
+S DQ+EG K W +T+ NF E+GRES SG *+ KGN P*I S DSKWKNHKAQEN*
Sbjct: 347 RS*DQTEGCKHIWSYTLANF*RNEEGRESKSGCY*SQKGNTP*I*SGDSKWKNHKAQEN* 526
Query: 418 EPCHVW*TELSETIA*YY*QRC*KTKKGSYKIPCTSCKHW*IIL*GFLESVC*RPLCF*L 239
E CHVW TE SE IA Y *Q C K KKG +IP SC++ *II *G LES+C* PLC L
Sbjct: 527 EHCHVWGTEYSEVIAKYC*QGCHKNKKGCCRIPSPSCQYR*IIF*GLLESIC**PLCIWL 706
Query: 238 EAHAW 224
EAH +
Sbjct: 707 EAHVY 721
Score = 158 bits (339), Expect = 6e-038
Identities = 78/123 (63%), Positives = 87/123 (70%)
Frame = +3 / -1
Query: 231 *ASSQKHKGRQHTDSRNPQRIIHQCLQDVHGIL*EPFLVF*HLCQ*YYAMVSESSVHQT* 410
*AS+Q HKG QH DSR+PQ+IIH+ QD GIL +PFL *H CQ Y+AM SE SV QT*
Sbjct: 714 *ASNQMHKGHQHMDSRSPQKIIHRYWQDGDGILQQPFLFL*HPCQQYFAMTSEYSVPQT* 535
Query: 411 QGS*FSCAL*FFHLLSQLRIQGGLPFYV*EYPETLSLPFSTCSKLVIVKSQVSLRPSDWS 590
Q S*FSCAL*FFHLLS L I G LPF * +P+ LSLP S KL V Q+ L PS WS
Sbjct: 534 QCS*FSCAL*FFHLLSPLHIHGVLPFCD**HPDLLSLPSSLR*KLASV*LQICLHPSVWS 355
Query: 591 PDL 599
DL
Sbjct: 354 QDL 346
Score = 138 bits (297), Expect = 4e-032
Identities = 70/109 (64%), Positives = 78/109 (71%)
Frame = -3 / +1
Query: 596 VRRPIGRT*TYLGFYNHQFRTC*ER*RKCLRIFLNIER*PTLNTKLRQQMEESQSAGKLG 417
V RP GR *TYL Y QF T * R*RK +R+ L ER* T+N K RQQME+SQSAGKL
Sbjct: 349 VLRPNGRM*TYLELYTRQFLT**GR*RKQVRMLLITER*HTMNMKWRQQMEKSQSAGKLR 528
Query: 416 ALSCLVNRTLRNHCIILLTKMLKN*KRFLQDPVHILQTLVNYSLRVP*I 270
ALSCL NR LR+HC ILLT+M + +R LQDPV IL VNY LR P*I
Sbjct: 529 ALSCLGNRILRSHCKILLTRMSQKQERLLQDPVPILPISVNYFLRAP*I 675
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 593,636,107
Number of Sequences: 636343
Number of extensions: 8780344
Number of successful extensions: 85030
Number of sequences better than 1.0e-020: 1
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 13303085100
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)