TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750560.2.1
(660 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA741533.1|CA741533 wia1c.pk003.i23 wia1c Triticum aesti... 153 4e-044
gb|BQ620658.1|BQ620658 TaLr1135G05R TaLr1 Triticum aestivum... 153 1e-041
gb|CA728774.1|CA728774 wdi1c.pk005.b12 wdi1c Triticum aesti... 157 1e-037
gb|CA497106.1|CA497106 WHE3223_B08_D15ZT Wheat meiotic anth... 156 2e-037
gb|BJ214159.1|BJ214159 BJ214159 Y. Ogihara unpublished cDNA... 70 2e-030
gb|BJ207936.1|BJ207936 BJ207936 Y. Ogihara unpublished cDNA... 84 6e-029
gb|BJ305337.1|BJ305337 BJ305337 Y. Ogihara unpublished cDNA... 72 2e-025
gb|BQ620202.1|BQ620202 TaLr1176E11R TaLr1 Triticum aestivum... 70 7e-025
gb|CF243394.1|CF243394 CCM10 Chinese Spring wheat immature ... 65 4e-023
gb|BJ234204.1|BJ234204 BJ234204 Y. Ogihara unpublished cDNA... 65 7e-023
gb|BQ578824.1|BQ578824 WHE0407_B10_D19ZS Wheat etiolated se... 65 9e-022
gb|BJ208165.1|BJ208165 BJ208165 Y. Ogihara unpublished cDNA... 70 3e-021
>gb|CA741533.1|CA741533 wia1c.pk003.i23 wia1c Triticum aestivum cDNA clone wia1c.pk003.i23
5' end, mRNA sequence
Length = 612
Score = 153 bits (329), Expect(2) = 4e-044
Identities = 58/87 (66%), Positives = 73/87 (83%)
Frame = +3 / +3
Query: 264 DALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKILCK 443
D L+ ELWHACAGPLVTVPR G+LV+YFPQGH+EQ+EAS +Q + L+DLP K+LC+
Sbjct: 204 DPLYDELWHACAGPLVTVPRVGDLVFYFPQGHIEQVEASMNQVAGNQMRLYDLPSKLLCR 383
Query: 444 VVNVELRAETDSDEVYAQIMLQPEADQ 524
V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 384 VINVELKAEADTDEVYAQVMLMPEPEQ 464
Score = 45.5 bits (93), Expect(2) = 4e-044
Identities = 20/26 (76%), Positives = 20/26 (76%)
Frame = +3 / +3
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHA 653
V SFCK LTASDTST SVLRRHA
Sbjct: 525 VRSFCKXLTASDTSTXXXXSVLRRHA 602
>gb|BQ620658.1|BQ620658 TaLr1135G05R TaLr1 Triticum aestivum cDNA clone TaLr1135G05R, mRNA
sequence
Length = 569
Score = 153 bits (328), Expect(2) = 1e-041
Identities = 58/87 (66%), Positives = 73/87 (83%)
Frame = +3 / +3
Query: 264 DALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLDQHLPLFDLPPKILCK 443
D L+ ELWHACAGPLVTVPR G+LV+YFPQGH+EQ+EAS +Q + L+DLP K+LC+
Sbjct: 201 DPLYDELWHACAGPLVTVPRVGDLVFYFPQGHIEQVEASMNQIAGNQMRLYDLPSKLLCR 380
Query: 444 VVNVELRAETDSDEVYAQIMLQPEADQ 524
V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 381 VINVELKAEADTDEVYAQVMLMPEPEQ 461
Score = 37.7 bits (76), Expect(2) = 1e-041
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = +3 / +3
Query: 576 VYSFCKTLTASDTSTH 623
V SFCKTLTASDTSTH
Sbjct: 522 VRSFCKTLTASDTSTH 569
>gb|CA728774.1|CA728774 wdi1c.pk005.b12 wdi1c Triticum aestivum cDNA clone wdi1c.pk005.b12
5' end, mRNA sequence
Length = 615
Score = 157 bits (337), Expect = 1e-037
Identities = 66/97 (68%), Positives = 72/97 (74%)
Frame = +3 / +1
Query: 189 FCSRLVSEAAEMXXXXXXXXXXXXXDALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQ 368
F S V+ A M DALFRELWHACAGPL+TVPRQGE VYYFPQGHMEQ
Sbjct: 322 FGSANVAAAMAMANPSAAGAPGICSDALFRELWHACAGPLITVPRQGERVYYFPQGHMEQ 501
Query: 369 LEASTDQQLDQHLPLFDLPPKILCKVVNVELRAETDS 479
LEAST+QQLDQ+LP+F+LP KILC VVNV LR E DS
Sbjct: 502 LEASTNQQLDQYLPMFNLPSKILCSVVNVXLRTEADS 612
>gb|CA497106.1|CA497106 WHE3223_B08_D15ZT Wheat meiotic anther cDNA library Triticum
aestivum cDNA clone WHE3223_B08_D15, mRNA sequence
Length = 425
Score = 156 bits (336), Expect = 2e-037
Identities = 66/96 (68%), Positives = 76/96 (79%)
Frame = +3 / +2
Query: 372 EASTDQQLDQHLPLFDLPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQXXXXXXXXX 551
++ST+QQLDQ+LP+F+LP KILC VVNVELR E DSDEVYAQIMLQPEA+Q
Sbjct: 53 DSSTNQQLDQYLPMFNLPSKILCSVVNVELRTEADSDEVYAQIMLQPEANQGELTSLGPE 232
Query: 552 XXXXXRCNVYSFCKTLTASDTSTHGGFSVLRRHAEE 659
+ ++SFCKTLTASDTSTHGGFSVLRRHAEE
Sbjct: 233 PKELEKGTIHSFCKTLTASDTSTHGGFSVLRRHAEE 340
Score = 127 bits (271), Expect = 2e-028
Identities = 56/96 (58%), Positives = 69/96 (71%)
Frame = -2 / -2
Query: 656 FSMPPEDRESTMGTCI*SSQGLAEGIDVASFRLRWLRIWAGGLTLICFWLQHNLSINFIR 477
FSMPP++RE+TMGT I S Q LA+G+ A F WLR A LTLI WLQHNLS+NFI
Sbjct: 337 FSMPPKNRETTMGTRIRSCQCLAKGMYGAFFEFFWLRTQACELTLISLWLQHNLSVNFI* 158
Query: 476 ISFSSKFYIDHLAENLGW*IKQR*VLIKLLICRCFK 369
ISF + Y+DH A + GW*++ R VL+KLL+CR K
Sbjct: 157 ISFRPELYVDHAA*DFGW*VEHRQVLVKLLVCR*IK 50
>gb|BJ214159.1|BJ214159 BJ214159 Y. Ogihara unpublished cDNA library, Wh Triticum aestivum
cDNA clone wh26b20 5', mRNA sequence
Length = 583
Score = 69.8 bits (146), Expect(3) = 2e-030
Identities = 26/44 (59%), Positives = 33/44 (75%)
Frame = +3 / +1
Query: 267 ALFRELWHACAGPLVTVPRQGELVYYFPQGHMEQLEASTDQQLD 398
A+ ELWHAC+GPLV +P G LV YFPQGH EQ+ AS +++D
Sbjct: 43 AINSELWHACSGPLVAMPPVGSLVVYFPQGHSEQVAASMHKEVD 174
Score = 53.8 bits (111), Expect(3) = 2e-030
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = +3 / +1
Query: 585 FCKTLTASDTSTHGGFSVLRRHAEE 659
FCKTLTASDTSTHGGFSV RR AE+
Sbjct: 364 FCKTLTASDTSTHGGFSVPRRAAEK 438
Score = 47.3 bits (97), Expect(3) = 2e-030
Identities = 16/31 (51%), Positives = 27/31 (87%)
Frame = +3 / +1
Query: 420 LPPKILCKVVNVELRAETDSDEVYAQIMLQP 512
LP K++CK++++ L A++++DEVYAQ+ LQP
Sbjct: 196 LPSKLICKLLSLTLHADSETDEVYAQMTLQP 288
>gb|BJ207936.1|BJ207936 BJ207936 Y. Ogihara unpublished cDNA library, Wh Triticum aestivum
cDNA clone wh6b03 5', mRNA sequence
Length = 408
Score = 83.5 bits (176), Expect(2) = 6e-029
Identities = 32/55 (58%), Positives = 44/55 (80%)
Frame = +3 / +2
Query: 360 MEQLEASTDQQLDQHLPLFDLPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
+EQ+EAS +Q + L+DLP K+LC+V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 14 IEQVEASMNQVAGNQMRLYDLPSKLLCRVINVELKAEADTDEVYAQVMLMPEPEQ 178
Score = 64.8 bits (135), Expect(2) = 6e-029
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +3 / +2
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 239 VRSFCKTLTASDTSTHGGFSVLRRHADE 322
>gb|BJ305337.1|BJ305337 BJ305337 Y. Ogihara unpublished cDNA library, Wh_yd Triticum
aestivum cDNA clone whyd29n08 5', mRNA sequence
Length = 649
Score = 71.6 bits (150), Expect(2) = 2e-025
Identities = 26/38 (68%), Positives = 34/38 (89%)
Frame = +3 / +1
Query: 411 LFDLPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
L+DLP K+LC+V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 43 LYDLPSKLLCRVINVELKAEADTDEVYAQVMLMPEPEQ 156
Score = 64.8 bits (135), Expect(2) = 2e-025
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +3 / +1
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 217 VRSFCKTLTASDTSTHGGFSVLRRHADE 300
>gb|BQ620202.1|BQ620202 TaLr1176E11R TaLr1 Triticum aestivum cDNA clone TaLr1176E11R, mRNA
sequence
Length = 702
Score = 69.8 bits (146), Expect(2) = 7e-025
Identities = 25/37 (67%), Positives = 33/37 (89%)
Frame = +3 / +1
Query: 414 FDLPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
+DLP K+LC+V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 16 YDLPSKLLCRVINVELKAEADTDEVYAQVMLMPEPEQ 126
Score = 64.8 bits (135), Expect(2) = 7e-025
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +3 / +1
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 187 VRSFCKTLTASDTSTHGGFSVLRRHADE 270
>gb|CF243394.1|CF243394 CCM10 Chinese Spring wheat immature spikelet cDNA Triticum aestivum
cDNA 5' similar to auxin response factor-like protein;
most similar to auxin response factor 9 and 1, mRNA
sequence
Length = 654
Score = 64.8 bits (135), Expect(3) = 4e-023
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +3 / +3
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 228 VRSFCKTLTASDTSTHGGFSVLRRHADE 311
Score = 50.6 bits (104), Expect(3) = 4e-023
Identities = 20/36 (55%), Positives = 28/36 (77%)
Frame = +3 / +1
Query: 363 EQLEASTDQQLDQHLPLFDLPPKILCKVVNVELRAE 470
EQ+EAS +Q + L+DLP K+LC+V+NVEL+AE
Sbjct: 1 EQVEASMNQVAANQMRLYDLPSKLLCRVLNVELKAE 108
Score = 30.9 bits (61), Expect(3) = 4e-023
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = +3 / +3
Query: 480 DEVYAQIMLQPEADQ 524
DEVYAQ+ML PE +Q
Sbjct: 120 DEVYAQVMLMPEPEQ 164
>gb|BJ234204.1|BJ234204 BJ234204 Y. Ogihara unpublished cDNA library, Wh_e Triticum
aestivum cDNA clone whe9b13 5', mRNA sequence
Length = 658
Score = 64.8 bits (135), Expect(2) = 7e-023
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +3 / +2
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 167 VRSFCKTLTASDTSTHGGFSVLRRHADE 250
Score = 62.9 bits (131), Expect(2) = 7e-023
Identities = 23/35 (65%), Positives = 30/35 (85%)
Frame = +3 / +2
Query: 420 LPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
LP +LC+V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 2 LPSXLLCRVINVELKAEADTDEVYAQVMLMPEPEQ 106
>gb|BQ578824.1|BQ578824 WHE0407_B10_D19ZS Wheat etiolated seedling root cDNA library
Triticum aestivum cDNA clone WHE0407_B10_D19, mRNA
sequence
Length = 780
Score = 64.8 bits (135), Expect(2) = 9e-022
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +3 / +2
Query: 576 VYSFCKTLTASDTSTHGGFSVLRRHAEE 659
V SFCKTLTASDTSTHGGFSVLRRHA+E
Sbjct: 179 VRSFCKTLTASDTSTHGGFSVLRRHADE 262
Score = 59.3 bits (123), Expect(2) = 9e-022
Identities = 21/32 (65%), Positives = 29/32 (90%)
Frame = +3 / +2
Query: 429 KILCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
++LC+V+NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 23 QLLCRVINVELKAEADTDEVYAQVMLMPEPEQ 118
>gb|BJ208165.1|BJ208165 BJ208165 Y. Ogihara unpublished cDNA library, Wh Triticum aestivum
cDNA clone wh7b03 5', mRNA sequence
Length = 248
Score = 69.8 bits (146), Expect(2) = 3e-021
Identities = 26/38 (68%), Positives = 33/38 (86%)
Frame = +3 / +1
Query: 411 LFDLPPKILCKVVNVELRAETDSDEVYAQIMLQPEADQ 524
L+DLP K+LC+V NVEL+AE D+DEVYAQ+ML PE +Q
Sbjct: 7 LYDLPSKLLCRVXNVELKAEADTDEVYAQVMLMPEPEQ 120
Score = 51.5 bits (106), Expect(2) = 3e-021
Identities = 21/22 (95%), Positives = 21/22 (95%)
Frame = +3 / +1
Query: 576 VYSFCKTLTASDTSTHGGFSVL 641
V SFCKTLTASDTSTHGGFSVL
Sbjct: 181 VRSFCKTLTASDTSTHGGFSVL 246
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 609,406,177
Number of Sequences: 636343
Number of extensions: 8196088
Number of successful extensions: 106184
Number of sequences better than 1.0e-020: 20
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 14722080844
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)