TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2655972.2.1
(666 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD898601.1|CD898601 G174.109I13F010824 G174 Triticum aes... 184 1e-052
gb|CK214143.1|CK214143 FGAS026066 Triticum aestivum FGAS: L... 144 3e-043
gb|CA633415.1|CA633415 wle1n.pk0070.f4 wle1n Triticum aesti... 137 1e-033
>gb|CD898601.1|CD898601 G174.109I13F010824 G174 Triticum aestivum cDNA clone G174109I13,
mRNA sequence
Length = 568
Score = 184 bits (397), Expect(2) = 1e-052
Identities = 75/92 (81%), Positives = 84/92 (91%)
Frame = +2 / +3
Query: 59 RNQENDFLYKDFWLTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKIKEQSSRLWNLL 238
RN++NDFLYKDFWL HAQD+GVLS K GGGFFVAFSRDQPQKVYVQ KIKEQS+R+WN+L
Sbjct: 12 RNEDNDFLYKDFWLKHAQDKGVLSLKEGGGFFVAFSRDQPQKVYVQHKIKEQSARVWNIL 191
Query: 239 LSDAVIYIAGSSTKMPADVTAALEEVICKEGG 334
S A IY+AGSSTKMPADVTAALEEVI ++GG
Sbjct: 192 CSGAAIYVAGSSTKMPADVTAALEEVIRQKGG 287
Score = 139 bits (298), Expect(2) = 2e-038
Identities = 65/93 (69%), Positives = 72/93 (77%)
Frame = -1 / -1
Query: 336 TPPSLQITSSSAAVTSAGIFVEEPAM*ITASDSNKFHSLELCSFILGCT*TFCG*SREKA 157
+PP +ITSSSAAVTSAGI VE+PA I A + N FH+L LCSFIL CT*TF G*S EKA
Sbjct: 289 SPPFWRITSSSAAVTSAGILVEDPATYIAAPEHNMFHTLALCSFILCCT*TF*G*SLEKA 110
Query: 156 TKKPPPLFEDNTPSSCA*VSQKSLYRKSFSWFL 58
TKKPPP F D+TP S A +QKSLYRKS S FL
Sbjct: 109 TKKPPPSFRDSTPLSWACFNQKSLYRKSLSSFL 11
Score = 113 bits (241), Expect(2) = 3e-029
Identities = 58/92 (63%), Positives = 62/92 (67%)
Frame = +3 / +1
Query: 60 ETKKTIFYTRISG*LMRRMRGCCPRKGAVVSSSLFPGISRKRSTCNPR*RNRVQDCGTYY 239
E K TIF TR SG* M R R CCP K AVVS FPGIS KRS N R*R+RVQ+CGTYY
Sbjct: 13 EMKTTIFCTRTSG*SMPRTRECCP*KRAVVSLLPFPGISLKRSMYNIR*RSRVQECGTYY 192
Query: 240 CPMQ*STLQALRRKCLPMLQLR*RRLFVKKVV 335
TLQ L KCL MLQL *++L KKVV
Sbjct: 193 ALGLQYTLQGLLPKCLLMLQLH*KKLSAKKVV 288
Score = 113 bits (241), Expect(2) = 3e-027
Identities = 48/91 (52%), Positives = 60/91 (65%)
Frame = +1 / +2
Query: 61 KPRKRFSIQGFLANSCAG*GGVVLEKGRWFLRRFFPGSAAKGLRATQDKGTEFKTVELIT 240
K R+RFS+QG L +C G G V E+GRWFL F GSA+KGL T DKG E K+VE I
Sbjct: 14 K*RQRFSVQGLLVEACPGQGSAVPERGRWFLCCLFQGSASKGLCTT*DKGAECKSVEHIM 193
Query: 241 VRCSDLHCRLFDENACRCYSCARGGYL*RRW 333
+ ++ CR+F +NAC CYSC R Y +RW
Sbjct: 194 LWGCNIRCRVFYQNAC*CYSCTRRSYPPKRW 286
Score = 106 bits (227), Expect(2) = 9e-025
Identities = 49/92 (53%), Positives = 61/92 (66%)
Frame = -2 / -2
Query: 335 HHLLYK*PPLAQL*HRQAFSSKSLQCRSLHRTVISSTVLNSVPLSWVARRPFAADPGKKR 156
HHL * L QL*H+QAF K+LQ +++I ST+L+S PLS+V RPF ADP K++
Sbjct: 288 HHLFGG*LLLVQL*HQQAFW*KTLQRILQPQSIICSTLLHSAPLSYVVHRPFEADPWKRQ 109
Query: 155 RRNHRPFSRTTPPHPAHELARNPCIENRFLGF 60
+RNHRP S T P P H R+PC ENR L F
Sbjct: 108 QRNHRPLSGTALPCPGHASTRSPCTENRCLHF 13
Score = 83.5 bits (176), Expect(3) = 4e-022
Identities = 37/61 (60%), Positives = 46/61 (75%)
Frame = -3 / -3
Query: 232 VPQS*TLFLYLGLHVDLLRLIPGKSDEETTAPFRGQHPLILRMS*PEILV*KIVFLVSTA 53
VP S TL LYL L++DLLRLIPGK ++ETTA F+GQH L+L M PE+LV KIV +S
Sbjct: 185 VPHSCTLLLYLMLYIDLLRLIPGKGNKETTALFQGQHSLVLGMLQPEVLVQKIVVFISRT 6
Query: 52 K 50
+
Sbjct: 5 R 3
Score = 42.8 bits (87), Expect(2) = 1e-052
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = +2 / +3
Query: 350 ASKWLRDLERAGRLNIETWS* 412
AS WLR LERAG+ NIETWS*
Sbjct: 294 ASGWLRKLERAGKFNIETWS* 356
Score = 40.9 bits (83), Expect(2) = 2e-038
Identities = 17/24 (70%), Positives = 21/24 (87%)
Frame = -1 / -1
Query: 414 NYDHVSMLSLPALSRSLSHFEASA 343
N+DHVSML+LPALS LSH EA++
Sbjct: 358 NHDHVSMLNLPALSSFLSHPEAAS 287
Score = 35.9 bits (72), Expect(2) = 3e-029
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +1 / +2
Query: 346 GCFEMAQGSRKGW*T*HRDMVIVVMGKK 429
GCF MAQ + KGW* *H ++V++ +
Sbjct: 290 GCFRMAQETGKGW*I*H*NVVVIFQSNR 373
Score = 34.1 bits (68), Expect(3) = 4e-022
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = -3 / -3
Query: 301 SCNIGRHFRRRACNV 257
SCNI RHF RR CNV
Sbjct: 254 SCNISRHFGRRPCNV 210
Score = 29.0 bits (57), Expect(2) = 3e-027
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = +3 / +1
Query: 345 RMLRNGSGI*KGLVDLTSRHG 407
R+L++GSG KGLV+LT + G
Sbjct: 289 RLLQDGSGNWKGLVNLTLKRG 351
Score = 27.2 bits (53), Expect(2) = 9e-025
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = -2 / -2
Query: 407 TMSRC*VYQPFLDP*AISKHP 345
T +C*+YQPF *AI K P
Sbjct: 351 TTFQC*IYQPFPVS*AILKQP 289
Score = 25.4 bits (49), Expect(3) = 4e-022
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3 / -3
Query: 406 PCLDVKSTSPF*IPEP 359
P +VK TSPF PEP
Sbjct: 350 PRFNVKFTSPFQFPEP 303
>gb|CK214143.1|CK214143 FGAS026066 Triticum aestivum FGAS: Library 6 CAP GATE 1 Triticum
aestivum cDNA, mRNA sequence
Length = 1039
Score = 144 bits (308), Expect(2) = 3e-043
Identities = 56/82 (68%), Positives = 66/82 (80%)
Frame = +2 / -1
Query: 32 SPVLFFFGCRNQENDFLYKDFWLTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKIKE 211
+PVLFFFGCRNQ+NDFLYKDFWL HAQD+GVLS ++GGG FVAFSRDQPQKVYVQ KI+E
Sbjct: 601 APVLFFFGCRNQDNDFLYKDFWLNHAQDQGVLSLEKGGGLFVAFSRDQPQKVYVQDKIRE 422
Query: 212 QSSRLWNLLLSDAVIYIAGSST 277
Q +R+ N+L S S+
Sbjct: 421 QGARVLNMLCSSEATIYVAGSS 356
Score = 105 bits (223), Expect = 7e-022
Identities = 48/94 (51%), Positives = 63/94 (67%)
Frame = -1 / +3
Query: 315 TSSSAAVTSAGIFVEEPAM*ITASDSNKFHSLELCSFILGCT*TFCG*SREKATKKPPPL 136
+S++A ++ + + + + + N F++L CS IL CT*TF G*S E AT KPPP
Sbjct: 318 SSNAAVTSAGILVEDPATYIVASEEHNMFNTLAPCSLILSCT*TF*G*SLENATNKPPPF 497
Query: 135 FEDNTPSSCA*VSQKSLYRKSFSWFLQPKKNRTG 34
++TP S A* +QKSLYRKS S FLQPKKNRTG
Sbjct: 498 SRESTPWSWA*FNQKSLYRKSLS*FLQPKKNRTG 599
Score = 94.1 bits (199), Expect(2) = 5e-033
Identities = 38/61 (62%), Positives = 50/61 (81%)
Frame = +2 / -1
Query: 233 LLLSDAVIYIAGSSTKMPADVTAALEEVICKEGGVKQADASKWLRDLERAGRLNIETWS* 412
L S+A IY+AGSST+MPADVTAALEEV+C++GGV + D S+W+ L++ G+ IETWS*
Sbjct: 397 LCSSEATIYVAGSSTRMPADVTAALEEVLCQQGGVPKNDLSQWVTGLKKNGKFIIETWS* 218
Query: 413 L 415
L
Sbjct: 217 L 215
Score = 85.4 bits (180), Expect(2) = 2e-030
Identities = 32/69 (46%), Positives = 44/69 (63%)
Frame = -2 / +1
Query: 245 RTVISSTVLNSVPLSWVARRPFAADPGKKRRRNHRPFSRTTPPHPAHELARNPCIENRFL 66
R++ ST+L+ P ++ RPF DP K ++ NH PF PP P H+L R+PC EN +L
Sbjct: 388 RSITCSTLLHPAP*FYLVHRPFEVDP*KTQQINHHPFLGKAPPGPGHDLTRSPCTENHYL 567
Query: 65 GFYSQKRTE 39
FYSQ+R E
Sbjct: 568 DFYSQRRIE 594
Score = 68.0 bits (142), Expect(2) = 5e-033
Identities = 36/71 (50%), Positives = 43/71 (60%)
Frame = +3 / -2
Query: 39 FCSFLAVETKKTIFYTRISG*LMRRMRGCCPRKGAVVSSSLFPGISRKRSTCNPR*RNRV 218
F S LAVE K IF TR SG*+M R RGC P K VV F GI+ KRS + +RV
Sbjct: 594 FYSSLAVEIKIMIFCTRTSG*IMPRTRGCFP*KRVVVYLLRFLGINLKRSMYKIKLGSRV 415
Query: 219 QDCGTYYCPMQ 251
Q+C T Y P++
Sbjct: 414 QEC*TCYAPLR 382
Score = 68.0 bits (142), Expect(2) = 2e-030
Identities = 34/71 (47%), Positives = 45/71 (63%)
Frame = -1 / +3
Query: 447 NINNMYFLAHHNYDHVSMLSLPALSRSLSHFEASACLTPPSLQITSSSAAVTSAGIFVEE 268
N N+ + +YD VSM++LP R ++H E S TPP TSS+AAVTSAGI VE+
Sbjct: 183 NNRNLIPNIYLSYDQVSMINLPFFFRPVTHCERSFFGTPPC*HKTSSNAAVTSAGILVED 362
Query: 267 PAM*ITASDSN 235
PA I AS+ +
Sbjct: 363 PATYIVASEEH 395
Score = 52.4 bits (108), Expect(2) = 3e-043
Identities = 20/53 (37%), Positives = 27/53 (50%)
Frame = +1 / -3
Query: 253 DLHCRLFDENACRCYSCARGGYL*RRWCQASGCFEMAQGSRKGW*T*HRDMVI 411
++ C +F +NAC CY C ++ WC F M GS K W H D+VI
Sbjct: 377 NIRCWVFYQNAC*CYGCIGRSFMSAGWCSKK*SFAMGDGSEKEWQVYH*DLVI 219
>gb|CA633415.1|CA633415 wle1n.pk0070.f4 wle1n Triticum aestivum cDNA clone wle1n.pk0070.f4
5' end, mRNA sequence
Length = 530
Score = 137 bits (294), Expect(2) = 1e-033
Identities = 54/82 (65%), Positives = 64/82 (78%)
Frame = +2 / -3
Query: 32 SPVLFFFGCRNQENDFLYKDFWLTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKIKE 211
+PVLFFFGCRNQ+ND LYKDFWL HAQD+GVLS ++GGG VAFSRDQPQKVYVQ KI+E
Sbjct: 303 APVLFFFGCRNQDNDXLYKDFWLNHAQDQGVLSLEKGGGLXVAFSRDQPQKVYVQDKIRE 124
Query: 212 QSSRLWNLLLSDAVIYIAGSST 277
Q +R+ N+L S S+
Sbjct: 123 QGARVLNMLCSSEATXYVAGSS 58
Score = 101 bits (216), Expect = 6e-021
Identities = 47/94 (50%), Positives = 62/94 (65%)
Frame = -1 / +2
Query: 315 TSSSAAVTSAGIFVEEPAM*ITASDSNKFHSLELCSFILGCT*TFCG*SREKATKKPPPL 136
+S++A ++ + + + + + N F++L CS IL CT*TF G*S E AT KPPP
Sbjct: 20 SSNAAVTSAGILVEDPATXIVASEEHNMFNTLAPCSLILSCT*TF*G*SLENATXKPPPF 199
Query: 135 FEDNTPSSCA*VSQKSLYRKSFSWFLQPKKNRTG 34
++TP S A* +QKSLYR S S FLQPKKNRTG
Sbjct: 200 SRESTPWSWA*FNQKSLYRXSLS*FLQPKKNRTG 301
Score = 81.3 bits (171), Expect(2) = 6e-021
Identities = 31/69 (44%), Positives = 42/69 (60%)
Frame = -2 / +3
Query: 245 RTVISSTVLNSVPLSWVARRPFAADPGKKRRRNHRPFSRTTPPHPAHELARNPCIENRFL 66
R++ ST+L+ P ++ RPF DP K + NH PF PP P H+L R+PC E +L
Sbjct: 90 RSITCSTLLHPAP*FYLVHRPFEVDP*KTQXINHHPFLGKAPPGPGHDLTRSPCTEXHYL 269
Query: 65 GFYSQKRTE 39
FYSQ+R E
Sbjct: 270 DFYSQRRIE 296
Score = 66.6 bits (139), Expect(2) = 3e-021
Identities = 35/71 (49%), Positives = 42/71 (59%)
Frame = +3 / -1
Query: 39 FCSFLAVETKKTIFYTRISG*LMRRMRGCCPRKGAVVSSSLFPGISRKRSTCNPR*RNRV 218
F S LAVE K F TR SG*+M R RGC P K VV F GI+ KRS + +RV
Sbjct: 296 FYSSLAVEIKIMXFCTRTSG*IMPRTRGCFP*KRVVVYXLRFLGINLKRSMYKIKLGSRV 117
Query: 219 QDCGTYYCPMQ 251
Q+C T Y P++
Sbjct: 116 QEC*TCYAPLR 84
Score = 55.6 bits (115), Expect(2) = 3e-021
Identities = 22/32 (68%), Positives = 28/32 (87%)
Frame = +2 / -3
Query: 233 LLLSDAVIYIAGSSTKMPADVTAALEEVICKE 328
L S+A Y+AGSST+MPADVTAALEEV+C++
Sbjct: 99 LCSSEATXYVAGSSTRMPADVTAALEEVLCQK 4
Score = 40.0 bits (81), Expect(2) = 6e-021
Identities = 18/27 (66%), Positives = 22/27 (81%)
Frame = -1 / +2
Query: 315 TSSSAAVTSAGIFVEEPAM*ITASDSN 235
TSS+AAVTSAGI VE+PA I AS+ +
Sbjct: 17 TSSNAAVTSAGILVEDPATXIVASEEH 97
Score = 26.7 bits (52), Expect(2) = 1e-033
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +1 / -2
Query: 253 DLHCRLFDENACRCYSC 303
+ C +F +NAC CY C
Sbjct: 79 NXRCWVFYQNAC*CYGC 29
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 685,184,542
Number of Sequences: 636343
Number of extensions: 11171783
Number of successful extensions: 114872
Number of sequences better than 1.0e-020: 4
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 14899455312
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)