TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1738863.2.3
(730 letters)
Database: Triticum_nucl_with_EST.fasta
636,343 sequences; 367,240,239 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA627079.1|CA627079 wl1n.pk152.g4 wl1n Triticum aestivum... 130 2e-044
gb|CD935588.1|CD935588 OV.101O13R010406 OV Triticum aestivu... 124 8e-034
gb|CK215156.1|CK215156 FGAS027108 Triticum aestivum FGAS: L... 124 9e-034
gb|CK163816.1|CK163816 FGAS016452 Triticum aestivum FGAS: L... 122 1e-033
gb|CD491670.1|CD491670 WHE2853_F02_L03ZT CS wheat unstresse... 124 1e-033
gb|CD928430.1|CD928430 GR45.104N11F010316 GR45 Triticum aes... 124 1e-033
>gb|CA627079.1|CA627079 wl1n.pk152.g4 wl1n Triticum aestivum cDNA clone wl1n.pk152.g4 5'
end, mRNA sequence
Length = 482
Score = 130 bits (279), Expect(3) = 2e-044
Identities = 52/52 (100%), Positives = 52/52 (100%)
Frame = +1 / +2
Query: 40 CPADSLKLGACVDLLGGLVHIGLGDPVVNKCCPVLEGLVELEAAVCLCTTIK 195
CPADSLKLGACVDLLGGLVHIGLGDPVVNKCCPVLEGLVELEAAVCLCTTIK
Sbjct: 221 CPADSLKLGACVDLLGGLVHIGLGDPVVNKCCPVLEGLVELEAAVCLCTTIK 376
Score = 122 bits (261), Expect(2) = 6e-037
Identities = 48/48 (100%), Positives = 48/48 (100%)
Frame = -2 / -2
Query: 231 QWQVEVDVEEPELDGGAEAHGRLQLHQPLQHRAALVHHGVAEADMHQP 88
QWQVEVDVEEPELDGGAEAHGRLQLHQPLQHRAALVHHGVAEADMHQP
Sbjct: 412 QWQVEVDVEEPELDGGAEAHGRLQLHQPLQHRAALVHHGVAEADMHQP 269
Score = 54.2 bits (112), Expect(3) = 2e-044
Identities = 20/23 (86%), Positives = 20/23 (86%)
Frame = +3 / +2
Query: 231 GAPAPPHLRQDAAAGLHLHRLIW 299
GAP PPHLRQDAAAGLHL RL W
Sbjct: 413 GAPTPPHLRQDAAAGLHLQRLXW 481
Score = 52.8 bits (109), Expect(2) = 6e-037
Identities = 21/23 (91%), Positives = 22/23 (95%)
Frame = -3 / -2
Query: 299 PDQTVQV*PGGGVLPQVRRSWSA 231
P QT+QV*PGGGVLPQVRRSWSA
Sbjct: 481 PXQTLQV*PGGGVLPQVRRSWSA 413
Score = 33.6 bits (67), Expect(3) = 2e-044
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = +3 / +1
Query: 3 ATEPDADAVVADVPG 47
ATEPDADAVVA VPG
Sbjct: 184 ATEPDADAVVAYVPG 228
>gb|CD935588.1|CD935588 OV.101O13R010406 OV Triticum aestivum cDNA clone OV101O13, mRNA
sequence
Length = 681
Score = 124 bits (266), Expect(3) = 8e-034
Identities = 47/52 (90%), Positives = 51/52 (98%)
Frame = +1 / -3
Query: 40 CPADSLKLGACVDLLGGLVHIGLGDPVVNKCCPVLEGLVELEAAVCLCTTIK 195
CP DSLK+GACVDLLGGLVH+GLGDPVVNKCCP+LEGLVELEAAVCLCTTI+
Sbjct: 565 CPVDSLKIGACVDLLGGLVHVGLGDPVVNKCCPLLEGLVELEAAVCLCTTIR 410
Score = 115 bits (246), Expect(3) = 1e-027
Identities = 50/79 (63%), Positives = 51/79 (64%)
Frame = -3 / +3
Query: 284 QV*PGGGVLPQVRRSWSASGXXXXXXXXXXXMVVQRHXXXXXXXXXXXTGQHLFTTGSPR 105
QV*P GGVLPQVRRSW A G MVV RH +GQHLFTTGSPR
Sbjct: 321 QV*PVGGVLPQVRRSWRARGRTRLMLRSFSRMVVHRHTAASSSTSPSSSGQHLFTTGSPR 500
Query: 104 PICTSPPSRSTHAPSFSES 48
P CTSPPSRSTHAP FSES
Sbjct: 501 PTCTSPPSRSTHAPIFSES 557
Score = 39.1 bits (79), Expect(3) = 8e-034
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +1 / -3
Query: 250 TCGKTPPPGYTCTV*S 297
TCGKTPP GYTC++*S
Sbjct: 355 TCGKTPPTGYTCSI*S 308
Score = 24.0 bits (46), Expect(3) = 1e-027
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -1 / +1
Query: 412 DILSCRSLFKRPM 374
DILSCRS+ + P+
Sbjct: 238 DILSCRSIHQSPL 276
Score = 22.1 bits (42), Expect(3) = 1e-027
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -3 / +3
Query: 485 LIDPAVVTLFLLRRAFFSLERRSRRHPLMSI 393
L D ++ + LR + ++ RHPLMSI
Sbjct: 165 LADRSISSSCSLRTSCLAIGDDDVRHPLMSI 257
Score = 21.7 bits (41), Expect(3) = 8e-034
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +3 / -2
Query: 21 DAVVADVPGGL 53
DA ADVPGGL
Sbjct: 584 DARGADVPGGL 552
>gb|CK215156.1|CK215156 FGAS027108 Triticum aestivum FGAS: Library 6 CAP GATE 1 Triticum
aestivum cDNA, mRNA sequence
Length = 745
Score = 124 bits (266), Expect(3) = 9e-034
Identities = 47/52 (90%), Positives = 51/52 (98%)
Frame = +1 / -1
Query: 40 CPADSLKLGACVDLLGGLVHIGLGDPVVNKCCPVLEGLVELEAAVCLCTTIK 195
CP DSLK+GACVDLLGGLVH+GLGDPVVNKCCP+LEGLVELEAAVCLCTTI+
Sbjct: 625 CPVDSLKIGACVDLLGGLVHVGLGDPVVNKCCPLLEGLVELEAAVCLCTTIR 470
Score = 115 bits (246), Expect(2) = 2e-026
Identities = 50/79 (63%), Positives = 51/79 (64%)
Frame = -3 / +3
Query: 284 QV*PGGGVLPQVRRSWSASGXXXXXXXXXXXMVVQRHXXXXXXXXXXXTGQHLFTTGSPR 105
QV*P GGVLPQVRRSW A G MVV RH +GQHLFTTGSPR
Sbjct: 381 QV*PVGGVLPQVRRSWRARGRTRLMLRSFSRMVVHRHTAASSSTSPSSSGQHLFTTGSPR 560
Query: 104 PICTSPPSRSTHAPSFSES 48
P CTSPPSRSTHAP FSES
Sbjct: 561 PTCTSPPSRSTHAPIFSES 617
Score = 39.1 bits (79), Expect(3) = 9e-034
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +1 / -1
Query: 250 TCGKTPPPGYTCTV*S 297
TCGKTPP GYTC++*S
Sbjct: 415 TCGKTPPTGYTCSI*S 368
Score = 24.0 bits (46), Expect(2) = 2e-026
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -1 / +1
Query: 412 DILSCRSLFKRPM 374
DILSCRS+ + P+
Sbjct: 298 DILSCRSIHQSPL 336
Score = 21.7 bits (41), Expect(3) = 9e-034
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +3 / -3
Query: 21 DAVVADVPGGL 53
DA ADVPGGL
Sbjct: 644 DARGADVPGGL 612
>gb|CK163816.1|CK163816 FGAS016452 Triticum aestivum FGAS: Library 4 Gate 8 Triticum
aestivum cDNA, mRNA sequence
Length = 1082
Score = 122 bits (262), Expect(2) = 1e-033
Identities = 47/52 (90%), Positives = 51/52 (98%)
Frame = +1 / -1
Query: 40 CPADSLKLGACVDLLGGLVHIGLGDPVVNKCCPVLEGLVELEAAVCLCTTIK 195
CP D+LKLGACVDLLGGLVHIGLGDPVVN+CCP+LEGLVELEAAVCLCTTI+
Sbjct: 551 CPVDTLKLGACVDLLGGLVHIGLGDPVVNQCCPLLEGLVELEAAVCLCTTIR 396
Score = 118 bits (253), Expect = 6e-026
Identities = 51/79 (64%), Positives = 52/79 (65%)
Frame = -3 / +1
Query: 284 QV*PGGGVLPQVRRSWSASGXXXXXXXXXXXMVVQRHXXXXXXXXXXXTGQHLFTTGSPR 105
QV PGGGVLPQVRRSW A G MVVQRH +GQH FTTGSPR
Sbjct: 307 QVKPGGGVLPQVRRSWRARGRARLMLRSFSRMVVQRHTAASSSTSPSSSGQHWFTTGSPR 486
Query: 104 PICTSPPSRSTHAPSFSES 48
PICTSPPSRSTHAPSFS S
Sbjct: 487 PICTSPPSRSTHAPSFSVS 543
Score = 40.9 bits (83), Expect(2) = 1e-033
Identities = 13/16 (81%), Positives = 16/16 (100%)
Frame = +1 / -1
Query: 250 TCGKTPPPGYTCTV*S 297
TCGKTPPPG+TC++*S
Sbjct: 341 TCGKTPPPGFTCSI*S 294
>gb|CD491670.1|CD491670 WHE2853_F02_L03ZT CS wheat unstressed root tip cDNA library
Triticum aestivum cDNA clone WHE2853_F02_L03, mRNA
sequence
Length = 703
Score = 124 bits (266), Expect(2) = 1e-033
Identities = 47/52 (90%), Positives = 51/52 (98%)
Frame = +1 / -1
Query: 40 CPADSLKLGACVDLLGGLVHIGLGDPVVNKCCPVLEGLVELEAAVCLCTTIK 195
CP DSLK+GACVDLLGGLVH+GLGDPVVNKCCP+LEGLVELEAAVCLCTTI+
Sbjct: 622 CPVDSLKIGACVDLLGGLVHVGLGDPVVNKCCPLLEGLVELEAAVCLCTTIR 467
Score = 115 bits (246), Expect(3) = 1e-027
Identities = 50/79 (63%), Positives = 51/79 (64%)
Frame = -3 / +3
Query: 284 QV*PGGGVLPQVRRSWSASGXXXXXXXXXXXMVVQRHXXXXXXXXXXXTGQHLFTTGSPR 105
QV*P GGVLPQVRRSW A G MVV RH +GQHLFTTGSPR
Sbjct: 378 QV*PVGGVLPQVRRSWRARGRTRLMLRSFSRMVVHRHTAASSSTSPSSSGQHLFTTGSPR 557
Query: 104 PICTSPPSRSTHAPSFSES 48
P CTSPPSRSTHAP FSES
Sbjct: 558 PTCTSPPSRSTHAPIFSES 614
Score = 39.1 bits (79), Expect(2) = 1e-033
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +1 / -1
Query: 250 TCGKTPPPGYTCTV*S 297
TCGKTPP GYTC++*S
Sbjct: 412 TCGKTPPTGYTCSI*S 365
Score = 24.0 bits (46), Expect(3) = 1e-027
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -1 / +1
Query: 412 DILSCRSLFKRPM 374
DILSCRS+ + P+
Sbjct: 295 DILSCRSIHQSPL 333
Score = 22.1 bits (42), Expect(3) = 1e-027
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -3 / +3
Query: 485 LIDPAVVTLFLLRRAFFSLERRSRRHPLMSI 393
L D ++ + LR + ++ RHPLMSI
Sbjct: 222 LADRSISSSCSLRTSCLAIGDDDVRHPLMSI 314
>gb|CD928430.1|CD928430 GR45.104N11F010316 GR45 Triticum aestivum cDNA clone GR45104N11,
mRNA sequence
Length = 677
Score = 124 bits (266), Expect(2) = 1e-033
Identities = 47/52 (90%), Positives = 51/52 (98%)
Frame = +1 / +1
Query: 40 CPADSLKLGACVDLLGGLVHIGLGDPVVNKCCPVLEGLVELEAAVCLCTTIK 195
CP DSLK+GACVDLLGGLVH+GLGDPVVNKCCP+LEGLVELEAAVCLCTTI+
Sbjct: 334 CPVDSLKIGACVDLLGGLVHVGLGDPVVNKCCPLLEGLVELEAAVCLCTTIR 489
Score = 109 bits (233), Expect = 3e-023
Identities = 48/79 (60%), Positives = 49/79 (62%)
Frame = -3 / -1
Query: 284 QV*PGGGVLPQVRRSWSASGXXXXXXXXXXXMVVQRHXXXXXXXXXXXTGQHLFTTGSPR 105
QV*P GGVLPQVRRSW A G MVV R +GQHLFTTGSP
Sbjct: 578 QV*PVGGVLPQVRRSWRARGRTRLMLSSLSRMVVHRQTAASSSTSPSSSGQHLFTTGSPS 399
Query: 104 PICTSPPSRSTHAPSFSES 48
P CTSPPSRSTHAP FSES
Sbjct: 398 PTCTSPPSRSTHAPIFSES 342
Score = 39.1 bits (79), Expect(2) = 1e-033
Identities = 13/16 (81%), Positives = 15/16 (93%)
Frame = +1 / +1
Query: 250 TCGKTPPPGYTCTV*S 297
TCGKTPP GYTC++*S
Sbjct: 544 TCGKTPPTGYTCSI*S 591
Database: Triticum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 3:01 PM
Number of letters in database: 367,240,239
Number of sequences in database: 636,343
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 653,861,666
Number of Sequences: 636343
Number of extensions: 9753601
Number of successful extensions: 118797
Number of sequences better than 1.0e-020: 9
length of database: 122,413,413
effective HSP length: 53
effective length of database: 88,687,234
effective search space used: 16761887226
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)