TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS16e03.yg.2.1
(608 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CL152970.1|CL152970 104_337_10780381_114_31367_013 Sorgh... 272 4e-072
gb|AW746450.1|AW746450 WS1_53_E10.b1_A002 Water-stressed 1 ... 210 1e-053
gb|CL155405.1|CL155405 104_341_10782104_116_31472_200 Sorgh... 142 2e-039
gb|CW277691.1|CW277691 104_751_11406162_116_35410_065 Sorgh... 133 2e-036
gb|BE919101.1|BE919101 FM1_3_B06.b1_A003 Floral-Induced Mer... 131 2e-033
gb|CW342955.1|CW342955 104_845_11487158_116_36188_063 Sorgh... 101 3e-027
>gb|CL152970.1|CL152970 104_337_10780381_114_31367_013 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10780381, DNA
sequence
Length = 482
Score = 272 bits (588), Expect = 4e-072
Identities = 102/120 (85%), Positives = 107/120 (89%)
Frame = -1 / -1
Query: 374 FCTHRIRLNKLDNIS*RYGCWNPVRCSVPCLHPFHASRSIHCSHPWGHWHWIKHQQMLGW 195
FCT IR+NKLDNI *RYGCWNP R SVPCLHPFHASRSIHCSHPWGHW+WIKHQQMLG
Sbjct: 482 FCTRCIRVNKLDNIR*RYGCWNPARWSVPCLHPFHASRSIHCSHPWGHWYWIKHQQMLGR 303
Query: 194 THEGRDWICK*TPSWFYFHLHCGSYEGPFQQK*E*IVRI*RDQCIGGRS*AGPCQGY*VP 15
HEGRDWICK*TPS YFHLHC +YEGP Q K*E*I+R+*RDQCIGGRS* GPCQGY*VP
Sbjct: 302 PHEGRDWICK*TPSRLYFHLHCSAYEGPLQWK*E*IIRV*RDQCIGGRS*TGPCQGY*VP 123
Score = 252 bits (545), Expect = 3e-066
Identities = 107/119 (89%), Positives = 110/119 (92%)
Frame = +3 / +3
Query: 18 YLVTLARTGL*STTNALIPLNSDDLFSFLLEWALVRTAVKVKVEPTWGLLANPISPLMSP 197
YLVTLARTGL*STTNALI LNSDDLFSF LEWALV TAVKVKV+PTWGLLANPISP M P
Sbjct: 126 YLVTLARTGL*STTNALISLNSDDLFSFPLEWALVSTAVKVKVQPTWGLLANPISPFMRP 305
Query: 198 TKHLLMLNPMPVPPWMRAMDGPTCMKGVKTRD*ASNGIPTPVSSTDIIKLIESDAMGAK 374
TKHLLMLNP+PVPPWMRAMDGPTCMKGVKTRD* S GIPTPVSS DIIKLI+SDA GAK
Sbjct: 306 TKHLLMLNPIPVPPWMRAMDGPTCMKGVKTRD*PSGGIPTPVSSPDIIKLIDSDAPGAK 482
Score = 249 bits (539), Expect = 2e-065
Identities = 109/119 (91%), Positives = 112/119 (94%)
Frame = -2 / -2
Query: 373 FAPIASDSISLIISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVG 194
FAP AS+SISLIIS EDTGVGIP D QSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVG
Sbjct: 481 FAPGASESISLIISGEDTGVGIPPDGQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVG 302
Query: 193 LMRGEIGFASKPQVGSTFTFTAVLTRAHSSRNENKSSEFKGINALVVDHRPVRAKVTRY 17
LM+GEIGFASKPQVG TFTFTAVLTRAHSS NENKSSEFK INALVVDHRPVRAKVT+Y
Sbjct: 301 LMKGEIGFASKPQVGCTFTFTAVLTRAHSSGNENKSSEFKEINALVVDHRPVRAKVTKY 125
Score = 232 bits (502), Expect = 3e-060
Identities = 99/119 (83%), Positives = 106/119 (89%)
Frame = +2 / +2
Query: 17 VPSNLGTDRPMIYHQCIDPFKF*RFILISAGMGPRKNRSEGESRTNLGFTCKSNLSPHES 196
V SNLGTDR MIYHQCID FK * FILIS G+GPRK+ SEGES T+LGFTCKSNLS HE+
Sbjct: 125 VLSNLGTDRSMIYHQCIDLFKL**FILISTGVGPRKHCSEGESTTDLGFTCKSNLSLHEA 304
Query: 197 NQAFADA*SNASAPMDASNGWTYLHERGEDTGLSIERDSNTRIFN*YYQAY*V*CDGCK 373
+QAFADA*SN SAPMDASNGWTYLHERGEDTGL+I RDSNTRIF *YYQAY*+*C GCK
Sbjct: 305 DQAFADA*SNTSAPMDASNGWTYLHERGEDTGLTIGRDSNTRIFT*YYQAY*L*CTGCK 481
Score = 220 bits (474), Expect = 2e-056
Identities = 99/119 (83%), Positives = 105/119 (88%)
Frame = -3 / -3
Query: 372 LHPSHQTQ*A**YQLKIRVLESRSMLSPVSSPLSCK*VHPLLASMGALALD*ASANAWLD 193
LHP HQ+Q*A**YQ+KIRVLESR M+SPVSSPLSCK*VHPLLASMGAL LD*ASANAW
Sbjct: 480 LHPVHQSQ*A**YQVKIRVLESRPMVSPVSSPLSCK*VHPLLASMGALVLD*ASANAWSA 301
Query: 192 S*GERLDLQVNPKLVLLSPSLRFLRGPIPAEMRINRQNLKGSMHWW*IIGRSVPRLLGT 16
S* ERLDLQVNPK V+LSPSL+ LRGP P EMRIN Q+LK SMHWW*II RSVPRLL T
Sbjct: 300 S*RERLDLQVNPKSVVLSPSLQCLRGPTPVEMRINHQSLKRSMHWW*IIDRSVPRLLST 124
Score = 215 bits (464), Expect = 5e-055
Identities = 92/119 (77%), Positives = 96/119 (80%)
Frame = +1 / +1
Query: 16 GT**PWHGPAYDLPPMH*SL*ILTIYSHFCWNGPS*EPQ*R*K*NQLGVYLQIQSLPS*V 195
GT**PWHGP YDLPPMH*SL* L IYSHF W+GPS* Q*R*K N+LGVYLQIQSLPS*
Sbjct: 124 GT**PWHGPVYDLPPMH*SL*TLMIYSHFHWSGPS*ALQ*R*KYNRLGVYLQIQSLPS*G 303
Query: 196 QPSIC*CLIQCQCPHGCEQWMDLLA*KG*RHGTEHRTGFQHPYXXXXXXXXXXXMRWVQ 372
+PSIC*CLIQ QCPHGCEQWMDLLA*KG*RHGT+HR GFQHPY M VQ
Sbjct: 304 RPSIC*CLIQYQCPHGCEQWMDLLA*KG*RHGTDHRAGFQHPYLHLILSSLLTLMHRVQ 480
>gb|AW746450.1|AW746450 WS1_53_E10.b1_A002 Water-stressed 1 (WS1) Sorghum bicolor cDNA,
mRNA sequence
Length = 545
Score = 210 bits (454), Expect = 1e-053
Identities = 78/89 (87%), Positives = 82/89 (92%)
Frame = -1 / +3
Query: 281 HPFHASRSIHCSHPWGHWHWIKHQQMLGWTHEGRDWICK*TPSWFYFHLHCGSYEGPFQQ 102
HPFHASRSIHCSHPWGHW+WIKHQQMLG HEGRDWICK*TPS FYFHLHC +YEGP Q
Sbjct: 9 HPFHASRSIHCSHPWGHWYWIKHQQMLGRPHEGRDWICK*TPSRFYFHLHCSAYEGPLQW 188
Query: 101 K*E*IVRI*RDQCIGGRS*AGPCQGY*VP 15
K*E*I+R+*RDQCIGGRS* GPCQGY*VP
Sbjct: 189 K*E*IIRV*RDQCIGGRS*TGPCQGY*VP 275
Score = 198 bits (427), Expect = 6e-050
Identities = 82/88 (93%), Positives = 83/88 (94%)
Frame = +3 / -1
Query: 18 YLVTLARTGL*STTNALIPLNSDDLFSFLLEWALVRTAVKVKVEPTWGLLANPISPLMSP 197
YLVTLARTGL*STTNALI LNSDDLFSF LEWALV TAVKVKVEPTWGLLANPISP M P
Sbjct: 272 YLVTLARTGL*STTNALISLNSDDLFSFPLEWALVSTAVKVKVEPTWGLLANPISPFMRP 93
Query: 198 TKHLLMLNPMPVPPWMRAMDGPTCMKGV 281
TKHLLMLNP+PVPPWMRAMDGPTCMKGV
Sbjct: 92 TKHLLMLNPIPVPPWMRAMDGPTCMKGV 9
Score = 198 bits (427), Expect = 6e-050
Identities = 84/88 (95%), Positives = 86/88 (97%)
Frame = -2 / +1
Query: 280 TPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGEIGFASKPQVGSTFTFTAVLTRAHSSR 101
TPFMQVGPSIARIHGGTGIGLSISKCLVGLM+GEIGFASKPQVGSTFTFTAVLTRAHSS
Sbjct: 10 TPFMQVGPSIARIHGGTGIGLSISKCLVGLMKGEIGFASKPQVGSTFTFTAVLTRAHSSG 189
Query: 100 NENKSSEFKGINALVVDHRPVRAKVTRY 17
NENKSSEFK INALVVDHRPVRAKVT+Y
Sbjct: 190 NENKSSEFKEINALVVDHRPVRAKVTKY 273
Score = 180 bits (387), Expect = 2e-044
Identities = 77/88 (87%), Positives = 80/88 (90%)
Frame = +1 / -2
Query: 16 GT**PWHGPAYDLPPMH*SL*ILTIYSHFCWNGPS*EPQ*R*K*NQLGVYLQIQSLPS*V 195
GT**PWHGP YDLPPMH*SL* L IYSHF W+GPS* Q*R*K*N+LGVYLQIQSLPS*
Sbjct: 274 GT**PWHGPVYDLPPMH*SL*TLMIYSHFHWSGPS*ALQ*R*K*NRLGVYLQIQSLPS*G 95
Query: 196 QPSIC*CLIQCQCPHGCEQWMDLLA*KG 279
+PSIC*CLIQ QCPHGCEQWMDLLA*KG
Sbjct: 94 RPSIC*CLIQYQCPHGCEQWMDLLA*KG 11
Score = 175 bits (376), Expect = 7e-043
Identities = 74/88 (84%), Positives = 79/88 (89%)
Frame = +2 / -3
Query: 17 VPSNLGTDRPMIYHQCIDPFKF*RFILISAGMGPRKNRSEGESRTNLGFTCKSNLSPHES 196
V SNLGTDR MIYHQCID FK * FILIS G+GPRK+ SEGESRT+LGFTCKSNLS HE+
Sbjct: 273 VLSNLGTDRSMIYHQCIDLFKL**FILISTGVGPRKHCSEGESRTDLGFTCKSNLSLHEA 94
Query: 197 NQAFADA*SNASAPMDASNGWTYLHERG 280
+QAFADA*SN SAPMDASNGWTYLHERG
Sbjct: 93 DQAFADA*SNTSAPMDASNGWTYLHERG 10
Score = 166 bits (358), Expect = 2e-040
Identities = 74/89 (83%), Positives = 77/89 (86%)
Frame = -3 / +2
Query: 282 SPLSCK*VHPLLASMGALALD*ASANAWLDS*GERLDLQVNPKLVLLSPSLRFLRGPIPA 103
+PLSCK*VHPLLASMGAL LD*ASANAW S* ERLDLQVNPK VLLSPSL+ LRGP P
Sbjct: 8 APLSCK*VHPLLASMGALVLD*ASANAWSAS*RERLDLQVNPKSVLLSPSLQCLRGPTPV 187
Query: 102 EMRINRQNLKGSMHWW*IIGRSVPRLLGT 16
EMRIN Q+LK SMHWW*II RSVPRLL T
Sbjct: 188 EMRINHQSLKRSMHWW*IIDRSVPRLLST 274
>gb|CL155405.1|CL155405 104_341_10782104_116_31472_200 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10782104, DNA
sequence
Length = 651
Score = 142 bits (305), Expect(2) = 2e-039
Identities = 58/85 (68%), Positives = 70/85 (82%)
Frame = -2 / -2
Query: 358 SDSISLIISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGE 179
SD ++L++SVEDTG+GIP DAQ++VFTPFMQ S +R +GGTGIGLSIS+CLV LM G+
Sbjct: 641 SDKVTLVVSVEDTGIGIPLDAQAKVFTPFMQADSSTSRTYGGTGIGLSISQCLVELMGGQ 462
Query: 178 IGFASKPQVGSTFTFTAVLTRAHSS 104
I F S+PQVGSTFTFTAVL R S
Sbjct: 461 INFVSRPQVGSTFTFTAVLQRCDRS 387
Score = 40.9 bits (83), Expect(2) = 2e-039
Identities = 16/23 (69%), Positives = 20/23 (86%)
Frame = -2 / -2
Query: 85 SEFKGINALVVDHRPVRAKVTRY 17
S FKG++AL+VD RPVRA VT+Y
Sbjct: 344 SSFKGLSALLVDTRPVRATVTKY 276
>gb|CW277691.1|CW277691 104_751_11406162_116_35410_065 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11406162, DNA
sequence
Length = 692
Score = 133 bits (284), Expect(2) = 2e-036
Identities = 55/78 (70%), Positives = 64/78 (82%)
Frame = -2 / +1
Query: 337 ISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGEIGFASKP 158
+SVEDTG+GIP DAQ++VFTPFMQ S +R +GGTGIGLSIS+CLV LM G+I F S+P
Sbjct: 1 VSVEDTGIGIPLDAQAKVFTPFMQADSSTSRTYGGTGIGLSISQCLVELMGGQINFVSRP 180
Query: 157 QVGSTFTFTAVLTRAHSS 104
QVGSTFTFTAVL R S
Sbjct: 181 QVGSTFTFTAVLQRCDRS 234
Score = 40.9 bits (83), Expect(2) = 2e-036
Identities = 16/23 (69%), Positives = 20/23 (86%)
Frame = -2 / +1
Query: 85 SEFKGINALVVDHRPVRAKVTRY 17
S FKG++AL+VD RPVRA VT+Y
Sbjct: 277 SSFKGLSALLVDTRPVRATVTKY 345
>gb|BE919101.1|BE919101 FM1_3_B06.b1_A003 Floral-Induced Meristem 1 (FM1) Sorghum
propinquum cDNA, mRNA sequence
Length = 521
Score = 131 bits (281), Expect(2) = 2e-033
Identities = 56/91 (61%), Positives = 68/91 (74%)
Frame = -2 / +1
Query: 358 SDSISLIISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGE 179
SD ++L IS+EDTGVGIP AQ RVFTPFMQ S +R +GGTGIGLSISKCL LM G+
Sbjct: 247 SDHVTLAISIEDTGVGIPLQAQDRVFTPFMQADSSTSRNYGGTGIGLSISKCLAELMGGQ 426
Query: 178 IGFASKPQVGSTFTFTAVLTRAHSSRNENKS 86
I F S+P VGSTFTF+A L R++ + + S
Sbjct: 427 ISFTSRPFVGSTFTFSATLKRSYKDISVDSS 519
Score = 31.8 bits (63), Expect(2) = 2e-033
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = -2 / +1
Query: 466 NTLSGYPVANRKRSWENFRV 407
NTLSG+ A+R+ SW+ F++
Sbjct: 124 NTLSGFEAADRRNSWQYFKL 183
>gb|CW342955.1|CW342955 104_845_11487158_116_36188_063 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11487158, DNA
sequence
Length = 583
Score = 101 bits (216), Expect(2) = 3e-027
Identities = 43/63 (68%), Positives = 50/63 (79%)
Frame = -2 / -3
Query: 292 SRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGEIGFASKPQVGSTFTFTAVLTRA 113
++VFTPFMQ S +R +GGTGIGLSIS+CLV LM G+I F S+PQVGSTFTFTAVL R
Sbjct: 581 AKVFTPFMQADSSTSRTYGGTGIGLSISQCLVELMGGQINFVSRPQVGSTFTFTAVLQRC 402
Query: 112 HSS 104
S
Sbjct: 401 DRS 393
Score = 40.9 bits (83), Expect(2) = 3e-027
Identities = 16/23 (69%), Positives = 20/23 (86%)
Frame = -2 / -3
Query: 85 SEFKGINALVVDHRPVRAKVTRY 17
S FKG++AL+VD RPVRA VT+Y
Sbjct: 350 SSFKGLSALLVDTRPVRATVTKY 282
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 661,434,436
Number of Sequences: 832831
Number of extensions: 9650770
Number of successful extensions: 87400
Number of sequences better than 1.0e-020: 7
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 17827330437
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)