TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH13e05.yg.2.1
(577 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW210433.1|CW210433 104_640_11189792_116_37000_018 Sorgh... 199 1e-062
gb|BG463534.1|BG463534 EM1_49_F12.g1_A002 Embryo 1 (EM1) So... 196 6e-062
gb|BE594757.1|BE594757 PI1_45_B05.g1_A002 Pathogen induced ... 173 4e-055
gb|BM327318.1|BM327318 PIC1_12_A06.g1_A002 Pathogen-infecte... 132 2e-031
gb|CW210434.1|CW210434 104_640_11189792_148_36999_018 Sorgh... 78 3e-028
gb|CW318924.1|CW318924 104_811_11474214_148_35883_025 Sorgh... 118 6e-026
gb|CF757196.1|CF757196 DSAF1_14_H08.g1_A011 Drought-stresse... 114 1e-024
gb|CF758925.1|CF758925 DSAF1_38_B12.g1_A011 Drought-stresse... 114 1e-024
gb|BM327323.1|BM327323 PIC1_12_A11.g1_A002 Pathogen-infecte... 55 1e-023
gb|CF072395.1|CF072395 FE1_2_F08.g1_A002 Iron-deficient see... 103 2e-021
>gb|CW210433.1|CW210433 104_640_11189792_116_37000_018 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11189792, DNA
sequence
Length = 644
Score = 199 bits (429), Expect(5) = 1e-062
Identities = 77/89 (86%), Positives = 83/89 (93%)
Frame = -1 / +3
Query: 451 SGELKMFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGYVIAHYQEPRDMMCLW 272
S L+MFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGYV+AHYQEPRDMMCLW
Sbjct: 126 SSLLQMFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGYVVAHYQEPRDMMCLW 305
Query: 271 DQFQKTKRGTCCKE*QKSCEWCFMLRWLL 185
D+FQKTKRGTCCKE*QK + CF+ RWL+
Sbjct: 306 DKFQKTKRGTCCKE*QKYWK*CFIFRWLV 392
Score = 175 bits (377), Expect(4) = 2e-050
Identities = 70/82 (85%), Positives = 75/82 (91%)
Frame = -3 / +2
Query: 437 DVQAGGCRNGNMDQ*DEEGWLGCQV*E*WEDFG*RMRGWLCDCSLPRTKGYDVPLGSVSE 258
DVQA GCRNGNMDQ*DEEGWLGCQV*E*WED G*R+RGW+C CSLPRTKGYDVPLG VSE
Sbjct: 140 DVQARGCRNGNMDQ*DEEGWLGCQV*E*WEDLG*RVRGWVCGCSLPRTKGYDVPLG*VSE 319
Query: 257 NKTRNMLQRITEIMRMVFYVTV 192
NKTRNMLQRITEI+ M+ Y +V
Sbjct: 320 NKTRNMLQRITEILEMMLYFSV 385
Score = 175 bits (376), Expect(5) = 5e-058
Identities = 76/88 (86%), Positives = 79/88 (89%)
Frame = +3 / -2
Query: 183 NSSHRNIKHHSHDFCYSLQHVPRFVF*N*SQRHIISLGSW**AIT*PSSHPSTKILPSFS 362
+++HR IKHH FCYSLQHVPRFVF*N SQRHIISLGSW**A T PSSHPSTKILPSFS
Sbjct: 394 STNHRKIKHHFQYFCYSLQHVPRFVF*NLSQRHIISLGSW**ATTYPSSHPSTKILPSFS 215
Query: 363 YLTSKPSFFISLIHIPIATSSSLNIFNS 446
YLTSKPSFFISLIHIPIATSSSLNI S
Sbjct: 214 YLTSKPSFFISLIHIPIATSSSLNICKS 131
Score = 98.7 bits (209), Expect(3) = 5e-026
Identities = 41/61 (67%), Positives = 43/61 (70%)
Frame = +2 / -1
Query: 260 LKLIPEAHHIPWFLVMSNHIAILASFNQNPPXXXXXXXXXXXXXXXDPYSHCDILQLEHL 439
LKLIPEAHHIPWFLV+SNHI ILA FNQ+PP DPYSHCDIL LEHL
Sbjct: 317 LKLIPEAHHIPWFLVVSNHIPILAPFNQDPPIILILDIQAILLHLIDPYSHCDIL*LEHL 138
Query: 440 * 442
*
Sbjct: 137 * 135
Score = 96.8 bits (205), Expect(3) = 2e-025
Identities = 41/51 (80%), Positives = 42/51 (82%)
Frame = -2 / +1
Query: 342 WLKDARMAM*LLITKNQGI*CASGISFRKQNEEHVAKNNRNHANGVLCYGG 190
WLK ARM M LL TKNQGI*CASGISFRKQNEEHVAKNNRN N L +GG
Sbjct: 235 WLKGARMGMWLLTTKNQGI*CASGISFRKQNEEHVAKNNRNTGNDALFFGG 387
Score = 92.3 bits (195), Expect(5) = 5e-031
Identities = 39/51 (76%), Positives = 43/51 (84%)
Frame = +1 / -3
Query: 193 TVT*NTIRMISVILCNMFLVLFSETDPRGTSYPLVLGNEQSHSHPRILQPK 345
T *+ I ISVILCNMFLVLFSET PRGTSYPLVLG+EQ H+HPR LQP+
Sbjct: 384 TEK*SIISSISVILCNMFLVLFSETYPRGTSYPLVLGSEQPHTHPRTLQPR 232
Score = 36.3 bits (73), Expect(5) = 5e-058
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1 / -1
Query: 133 VYIKNVLA*RCLFKQRP 183
VYIKNVLA*RCLF Q P
Sbjct: 431 VYIKNVLA*RCLFNQPP 381
Score = 36.3 bits (73), Expect(5) = 5e-058
Identities = 15/21 (71%), Positives = 19/21 (90%)
Frame = +3 / -1
Query: 15 MP*FDLKLFKLQTKQT*RYPK 77
+P*FDLKLF+L+TKQT*+ K
Sbjct: 542 IP*FDLKLFRLRTKQT*KISK 480
Score = 34.1 bits (68), Expect(5) = 1e-062
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -3 / +1
Query: 182 GRCLNRHLHAKTFLMYT 132
G LNRH HAKTFL+YT
Sbjct: 382 GGWLNRHRHAKTFLIYT 432
Score = 33.6 bits (67), Expect(5) = 5e-031
Identities = 14/19 (73%), Positives = 18/19 (94%)
Frame = +2 / -3
Query: 11 SHAVV*LETLQTADETNLK 67
++ +V*LETLQTADETNL+
Sbjct: 546 AYPLV*LETLQTADETNLE 490
Score = 31.8 bits (63), Expect(5) = 5e-031
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +3 / -3
Query: 132 CIHQERFSVKVSIQAT 179
CI+QERFSV VS+Q T
Sbjct: 432 CIYQERFSVTVSVQPT 385
Score = 30.9 bits (61), Expect(5) = 1e-062
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = -1 / +1
Query: 61 VCFVCSLKSFKSNYGM 14
VCFV SLKSFKSN G+
Sbjct: 496 VCFVRSLKSFKSN*GI 543
Score = 30.4 bits (60), Expect(4) = 2e-050
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -2 / +2
Query: 63 KFVSSAV*RVSSQTTAW 13
KFVSSAV*RVSSQT +
Sbjct: 494 KFVSSAV*RVSSQTRGY 544
Score = 30.4 bits (60), Expect(3) = 2e-025
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / +1
Query: 438 RCSSWRMSQWEY 403
RCSS RMSQWEY
Sbjct: 139 RCSS*RMSQWEY 174
Score = 29.9 bits (59), Expect(4) = 2e-050
Identities = 12/16 (75%), Positives = 15/16 (93%)
Frame = -2 / +3
Query: 177 LLE*TPSR*NVLDVYS 130
L+E TPSR*NVLD+Y+
Sbjct: 387 LVEQTPSR*NVLDIYT 434
Score = 29.9 bits (59), Expect(3) = 5e-026
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +1 / -2
Query: 22 SLT*NSSNCRRNKLEDIQKI 81
SLT*NSS+C RNKL K+
Sbjct: 535 SLT*NSSDCGRNKLRRYPKL 476
Score = 28.6 bits (56), Expect(5) = 1e-062
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -3 / +3
Query: 140 MYTALPKRILKELE 99
+YTALPKRIL E+E
Sbjct: 426 IYTALPKRILAEVE 467
Score = 27.6 bits (54), Expect(3) = 5e-026
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = +2 / -2
Query: 131 LYTSRTF*REGVYSSN 178
+Y SRTF*R+GV S+N
Sbjct: 433 VYISRTF*RDGVCSTN 386
Score = 27.6 bits (54), Expect(5) = 5e-058
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1 / -2
Query: 100 SSSFRILLGRAVYI 141
S+S RILLGRAVYI
Sbjct: 466 STSARILLGRAVYI 425
Score = 27.2 bits (53), Expect(3) = 2e-025
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = -3 / +3
Query: 62 SLFRLQFEEFQVKL 21
SLFR Q EEFQVKL
Sbjct: 495 SLFRPQSEEFQVKL 536
Score = 27.2 bits (53), Expect(5) = 5e-031
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +1 / -3
Query: 403 IFPLRHPPA*TSL 441
IFPLRHP A*TS+
Sbjct: 174 IFPLRHPLA*TSV 136
Score = 24.9 bits (48), Expect(5) = 5e-058
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +1 / -3
Query: 61 LEDIQKIVCLP 93
LEDIQ VCLP
Sbjct: 495 LEDIQNFVCLP 463
Score = 23.1 bits (44), Expect(5) = 1e-062
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -3 / +2
Query: 92 GKQTIFWISS 63
G+QT FWISS
Sbjct: 464 GRQTKFWISS 493
Score = 22.6 bits (43), Expect(5) = 5e-031
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +3 / -1
Query: 93 FQFQLF*NPLRQGCIH 140
F F NPLR GC++
Sbjct: 473 FAFHFCKNPLR*GCVY 426
Score = 21.7 bits (41), Expect(4) = 2e-050
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -2 / +2
Query: 138 VYSPA*EDSKRAG 100
++SP *EDS R+G
Sbjct: 428 IHSPT*EDSCRSG 466
>gb|BG463534.1|BG463534 EM1_49_F12.g1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 616
Score = 196 bits (423), Expect(5) = 6e-062
Identities = 75/84 (89%), Positives = 80/84 (95%)
Frame = -1 / +3
Query: 436 MFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGYVIAHYQEPRDMMCLWDQFQK 257
MFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGYV+AHYQEPRDMMCLWD+FQK
Sbjct: 3 MFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGYVVAHYQEPRDMMCLWDKFQK 182
Query: 256 TKRGTCCKE*QKSCEWCFMLRWLL 185
TKRGTCCKE*QK + CF+ RWL+
Sbjct: 183 TKRGTCCKE*QKYWK*CFIFRWLV 254
Score = 175 bits (377), Expect(4) = 2e-050
Identities = 70/82 (85%), Positives = 75/82 (91%)
Frame = -3 / +2
Query: 437 DVQAGGCRNGNMDQ*DEEGWLGCQV*E*WEDFG*RMRGWLCDCSLPRTKGYDVPLGSVSE 258
DVQA GCRNGNMDQ*DEEGWLGCQV*E*WED G*R+RGW+C CSLPRTKGYDVPLG VSE
Sbjct: 2 DVQARGCRNGNMDQ*DEEGWLGCQV*E*WEDLG*RVRGWVCGCSLPRTKGYDVPLG*VSE 181
Query: 257 NKTRNMLQRITEIMRMVFYVTV 192
NKTRNMLQRITEI+ M+ Y +V
Sbjct: 182 NKTRNMLQRITEILEMMLYFSV 247
Score = 174 bits (374), Expect(5) = 7e-058
Identities = 75/85 (88%), Positives = 78/85 (91%)
Frame = +3 / -1
Query: 183 NSSHRNIKHHSHDFCYSLQHVPRFVF*N*SQRHIISLGSW**AIT*PSSHPSTKILPSFS 362
+++HR IKHH FCYSLQHVPRFVF*N SQRHIISLGSW**A T PSSHPSTKILPSFS
Sbjct: 256 STNHRKIKHHFQYFCYSLQHVPRFVF*NLSQRHIISLGSW**ATTYPSSHPSTKILPSFS 77
Query: 363 YLTSKPSFFISLIHIPIATSSSLNI 437
YLTSKPSFFISLIHIPIATSSSLNI
Sbjct: 76 YLTSKPSFFISLIHIPIATSSSLNI 2
Score = 96.8 bits (205), Expect(3) = 2e-025
Identities = 41/51 (80%), Positives = 42/51 (82%)
Frame = -2 / +1
Query: 342 WLKDARMAM*LLITKNQGI*CASGISFRKQNEEHVAKNNRNHANGVLCYGG 190
WLK ARM M LL TKNQGI*CASGISFRKQNEEHVAKNNRN N L +GG
Sbjct: 97 WLKGARMGMWLLTTKNQGI*CASGISFRKQNEEHVAKNNRNTGNDALFFGG 249
Score = 96.4 bits (204), Expect(3) = 2e-025
Identities = 39/59 (66%), Positives = 41/59 (69%)
Frame = +2 / -3
Query: 260 LKLIPEAHHIPWFLVMSNHIAILASFNQNPPXXXXXXXXXXXXXXXDPYSHCDILQLEH 436
LKLIPEAHHIPWFLV+SNHI ILA FNQ+PP DPYSHCDIL LEH
Sbjct: 179 LKLIPEAHHIPWFLVVSNHIPILAPFNQDPPIILILDIQAILLHLIDPYSHCDIL*LEH 3
Score = 92.3 bits (195), Expect(5) = 6e-031
Identities = 39/51 (76%), Positives = 43/51 (84%)
Frame = +1 / -2
Query: 193 TVT*NTIRMISVILCNMFLVLFSETDPRGTSYPLVLGNEQSHSHPRILQPK 345
T *+ I ISVILCNMFLVLFSET PRGTSYPLVLG+EQ H+HPR LQP+
Sbjct: 246 TEK*SIISSISVILCNMFLVLFSETYPRGTSYPLVLGSEQPHTHPRTLQPR 94
Score = 36.3 bits (73), Expect(5) = 7e-058
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1 / -3
Query: 133 VYIKNVLA*RCLFKQRP 183
VYIKNVLA*RCLF Q P
Sbjct: 293 VYIKNVLA*RCLFNQPP 243
Score = 36.3 bits (73), Expect(5) = 7e-058
Identities = 15/21 (71%), Positives = 19/21 (90%)
Frame = +3 / -3
Query: 15 MP*FDLKLFKLQTKQT*RYPK 77
+P*FDLKLF+L+TKQT*+ K
Sbjct: 404 IP*FDLKLFRLRTKQT*KISK 342
Score = 34.1 bits (68), Expect(5) = 6e-062
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -3 / +1
Query: 182 GRCLNRHLHAKTFLMYT 132
G LNRH HAKTFL+YT
Sbjct: 244 GGWLNRHRHAKTFLIYT 294
Score = 33.6 bits (67), Expect(5) = 6e-031
Identities = 14/19 (73%), Positives = 18/19 (94%)
Frame = +2 / -2
Query: 11 SHAVV*LETLQTADETNLK 67
++ +V*LETLQTADETNL+
Sbjct: 408 AYPLV*LETLQTADETNLE 352
Score = 31.8 bits (63), Expect(5) = 6e-031
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +3 / -2
Query: 132 CIHQERFSVKVSIQAT 179
CI+QERFSV VS+Q T
Sbjct: 294 CIYQERFSVTVSVQPT 247
Score = 30.9 bits (61), Expect(5) = 6e-062
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = -1 / +1
Query: 61 VCFVCSLKSFKSNYGM 14
VCFV SLKSFKSN G+
Sbjct: 358 VCFVRSLKSFKSN*GI 405
Score = 30.4 bits (60), Expect(4) = 2e-050
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -2 / +2
Query: 63 KFVSSAV*RVSSQTTAW 13
KFVSSAV*RVSSQT +
Sbjct: 356 KFVSSAV*RVSSQTRGY 406
Score = 30.4 bits (60), Expect(3) = 2e-025
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / +1
Query: 438 RCSSWRMSQWEY 403
RCSS RMSQWEY
Sbjct: 1 RCSS*RMSQWEY 36
Score = 29.9 bits (59), Expect(4) = 2e-050
Identities = 12/16 (75%), Positives = 15/16 (93%)
Frame = -2 / +3
Query: 177 LLE*TPSR*NVLDVYS 130
L+E TPSR*NVLD+Y+
Sbjct: 249 LVEQTPSR*NVLDIYT 296
Score = 29.9 bits (59), Expect(3) = 2e-025
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +1 / -1
Query: 22 SLT*NSSNCRRNKLEDIQKI 81
SLT*NSS+C RNKL K+
Sbjct: 397 SLT*NSSDCGRNKLRRYPKL 338
Score = 28.6 bits (56), Expect(5) = 6e-062
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -3 / +3
Query: 140 MYTALPKRILKELE 99
+YTALPKRIL E+E
Sbjct: 288 IYTALPKRILAEVE 329
Score = 27.6 bits (54), Expect(3) = 2e-025
Identities = 11/16 (68%), Positives = 14/16 (87%)
Frame = +2 / -1
Query: 131 LYTSRTF*REGVYSSN 178
+Y SRTF*R+GV S+N
Sbjct: 295 VYISRTF*RDGVCSTN 248
Score = 27.6 bits (54), Expect(5) = 7e-058
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1 / -1
Query: 100 SSSFRILLGRAVYI 141
S+S RILLGRAVYI
Sbjct: 328 STSARILLGRAVYI 287
Score = 27.2 bits (53), Expect(3) = 2e-025
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = -3 / +3
Query: 62 SLFRLQFEEFQVKL 21
SLFR Q EEFQVKL
Sbjct: 357 SLFRPQSEEFQVKL 398
Score = 26.7 bits (52), Expect(5) = 6e-031
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +1 / -2
Query: 403 IFPLRHPPA*TS 438
IFPLRHP A*TS
Sbjct: 36 IFPLRHPLA*TS 1
Score = 24.9 bits (48), Expect(5) = 7e-058
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +1 / -2
Query: 61 LEDIQKIVCLP 93
LEDIQ VCLP
Sbjct: 357 LEDIQNFVCLP 325
Score = 23.1 bits (44), Expect(5) = 6e-062
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -3 / +2
Query: 92 GKQTIFWISS 63
G+QT FWISS
Sbjct: 326 GRQTKFWISS 355
Score = 22.6 bits (43), Expect(5) = 6e-031
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +3 / -3
Query: 93 FQFQLF*NPLRQGCIH 140
F F NPLR GC++
Sbjct: 335 FAFHFCKNPLR*GCVY 288
Score = 21.7 bits (41), Expect(4) = 2e-050
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -2 / +2
Query: 138 VYSPA*EDSKRAG 100
++SP *EDS R+G
Sbjct: 290 IHSPT*EDSCRSG 328
>gb|BE594757.1|BE594757 PI1_45_B05.g1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA,
mRNA sequence
Length = 636
Score = 173 bits (373), Expect(5) = 4e-055
Identities = 65/74 (87%), Positives = 70/74 (94%)
Frame = -1 / +1
Query: 406 IWINEMKKDGLDVKYENDGRILVEGCEDGYVIAHYQEPRDMMCLWDQFQKTKRGTCCKE* 227
IWINEMKKDGLDVKYENDGRILVEGCEDGYV+AHYQEPRDMMCLWD+FQKTKRGTCCKE*
Sbjct: 1 IWINEMKKDGLDVKYENDGRILVEGCEDGYVVAHYQEPRDMMCLWDKFQKTKRGTCCKE* 180
Query: 226 QKSCEWCFMLRWLL 185
QK + CF+ RWL+
Sbjct: 181 QKYWK*CFIFRWLV 222
Score = 151 bits (324), Expect(5) = 4e-051
Identities = 64/74 (86%), Positives = 67/74 (90%)
Frame = +3 / -2
Query: 183 NSSHRNIKHHSHDFCYSLQHVPRFVF*N*SQRHIISLGSW**AIT*PSSHPSTKILPSFS 362
+++HR IKHH FCYSLQHVPRFVF*N SQRHIISLGSW**A T PSSHPSTKILPSFS
Sbjct: 224 STNHRKIKHHFQYFCYSLQHVPRFVF*NLSQRHIISLGSW**ATTYPSSHPSTKILPSFS 45
Query: 363 YLTSKPSFFISLIH 404
YLTSKPSFFISLIH
Sbjct: 44 YLTSKPSFFISLIH 3
Score = 150 bits (322), Expect(4) = 6e-043
Identities = 60/71 (84%), Positives = 65/71 (91%)
Frame = -3 / +3
Query: 404 MDQ*DEEGWLGCQV*E*WEDFG*RMRGWLCDCSLPRTKGYDVPLGSVSENKTRNMLQRIT 225
MDQ*DEEGWLGCQV*E*WED G*R+RGW+C CSLPRTKGYDVPLG VSENKTRNMLQRIT
Sbjct: 3 MDQ*DEEGWLGCQV*E*WEDLG*RVRGWVCGCSLPRTKGYDVPLG*VSENKTRNMLQRIT 182
Query: 224 EIMRMVFYVTV 192
EI+ M+ Y +V
Sbjct: 183 EILEMMLYFSV 215
Score = 96.8 bits (205), Expect(2) = 1e-021
Identities = 41/51 (80%), Positives = 42/51 (82%)
Frame = -2 / +2
Query: 342 WLKDARMAM*LLITKNQGI*CASGISFRKQNEEHVAKNNRNHANGVLCYGG 190
WLK ARM M LL TKNQGI*CASGISFRKQNEEHVAKNNRN N L +GG
Sbjct: 65 WLKGARMGMWLLTTKNQGI*CASGISFRKQNEEHVAKNNRNTGNDALFFGG 217
Score = 92.3 bits (195), Expect(4) = 5e-028
Identities = 39/51 (76%), Positives = 43/51 (84%)
Frame = +1 / -3
Query: 193 TVT*NTIRMISVILCNMFLVLFSETDPRGTSYPLVLGNEQSHSHPRILQPK 345
T *+ I ISVILCNMFLVLFSET PRGTSYPLVLG+EQ H+HPR LQP+
Sbjct: 214 TEK*SIISSISVILCNMFLVLFSETYPRGTSYPLVLGSEQPHTHPRTLQPR 62
Score = 36.3 bits (73), Expect(5) = 4e-051
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1 / -1
Query: 133 VYIKNVLA*RCLFKQRP 183
VYIKNVLA*RCLF Q P
Sbjct: 261 VYIKNVLA*RCLFNQPP 211
Score = 36.3 bits (73), Expect(5) = 4e-051
Identities = 15/21 (71%), Positives = 19/21 (90%)
Frame = +3 / -1
Query: 15 MP*FDLKLFKLQTKQT*RYPK 77
+P*FDLKLF+L+TKQT*+ K
Sbjct: 372 IP*FDLKLFRLRTKQT*KISK 310
Score = 34.1 bits (68), Expect(5) = 4e-055
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -3 / +2
Query: 182 GRCLNRHLHAKTFLMYT 132
G LNRH HAKTFL+YT
Sbjct: 212 GGWLNRHRHAKTFLIYT 262
Score = 33.6 bits (67), Expect(4) = 5e-028
Identities = 14/19 (73%), Positives = 18/19 (94%)
Frame = +2 / -3
Query: 11 SHAVV*LETLQTADETNLK 67
++ +V*LETLQTADETNL+
Sbjct: 376 AYPLV*LETLQTADETNLE 320
Score = 31.8 bits (63), Expect(4) = 5e-028
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +3 / -3
Query: 132 CIHQERFSVKVSIQAT 179
CI+QERFSV VS+Q T
Sbjct: 262 CIYQERFSVTVSVQPT 215
Score = 30.9 bits (61), Expect(5) = 4e-055
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = -1 / +2
Query: 61 VCFVCSLKSFKSNYGM 14
VCFV SLKSFKSN G+
Sbjct: 326 VCFVRSLKSFKSN*GI 373
Score = 30.4 bits (60), Expect(4) = 6e-043
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -2 / +3
Query: 63 KFVSSAV*RVSSQTTAW 13
KFVSSAV*RVSSQT +
Sbjct: 324 KFVSSAV*RVSSQTRGY 374
Score = 29.9 bits (59), Expect(4) = 6e-043
Identities = 12/16 (75%), Positives = 15/16 (93%)
Frame = -2 / +1
Query: 177 LLE*TPSR*NVLDVYS 130
L+E TPSR*NVLD+Y+
Sbjct: 217 LVEQTPSR*NVLDIYT 264
Score = 28.6 bits (56), Expect(5) = 4e-055
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -3 / +1
Query: 140 MYTALPKRILKELE 99
+YTALPKRIL E+E
Sbjct: 256 IYTALPKRILAEVE 297
Score = 27.6 bits (54), Expect(5) = 4e-051
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1 / -2
Query: 100 SSSFRILLGRAVYI 141
S+S RILLGRAVYI
Sbjct: 296 STSARILLGRAVYI 255
Score = 27.2 bits (53), Expect(2) = 1e-021
Identities = 12/14 (85%), Positives = 12/14 (85%)
Frame = -3 / +1
Query: 62 SLFRLQFEEFQVKL 21
SLFR Q EEFQVKL
Sbjct: 325 SLFRPQSEEFQVKL 366
Score = 24.9 bits (48), Expect(5) = 4e-051
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +1 / -3
Query: 61 LEDIQKIVCLP 93
LEDIQ VCLP
Sbjct: 325 LEDIQNFVCLP 293
Score = 23.1 bits (44), Expect(5) = 4e-055
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -3 / +3
Query: 92 GKQTIFWISS 63
G+QT FWISS
Sbjct: 294 GRQTKFWISS 323
Score = 22.6 bits (43), Expect(4) = 5e-028
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +3 / -1
Query: 93 FQFQLF*NPLRQGCIH 140
F F NPLR GC++
Sbjct: 303 FAFHFCKNPLR*GCVY 256
Score = 21.7 bits (41), Expect(4) = 6e-043
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -2 / +3
Query: 138 VYSPA*EDSKRAG 100
++SP *EDS R+G
Sbjct: 258 IHSPT*EDSCRSG 296
>gb|BM327318.1|BM327318 PIC1_12_A06.g1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 622
Score = 132 bits (282), Expect(2) = 2e-031
Identities = 51/94 (54%), Positives = 64/94 (68%)
Frame = -1 / +1
Query: 514 AHGPGYIVSKDIAKEVYRKHKSGELKMFKLEDVAMGIWINEMKKDGLDVKYENDGRILVE 335
AHGPGYI+S+DIAK V R H+ L++FKLEDVAMGIWI + K G V D R E
Sbjct: 70 AHGPGYIISRDIAKFVVRGHQELTLQLFKLEDVAMGIWIQQYKNSGQQVNIVTDDRFYNE 249
Query: 334 GCEDGYVIAHYQEPRDMMCLWDQFQKTKRGTCCK 233
GC+ YV+AHYQ PR MMCLW++ + + CC+
Sbjct: 250 GCDADYVLAHYQTPRLMMCLWEKLKTEYQAVCCE 351
Score = 24.4 bits (47), Expect(2) = 2e-031
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = -3 / +3
Query: 569 *QVVHNP*GMA*GELSSLGAWTRIY 495
*Q+V+ GMA + ++G+W+ IY
Sbjct: 15 *QMVYQSEGMAFRHVPAMGSWSWIY 89
>gb|CW210434.1|CW210434 104_640_11189792_148_36999_018 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11189792, DNA
sequence
Length = 659
Score = 77.6 bits (163), Expect(5) = 4e-027
Identities = 28/36 (77%), Positives = 32/36 (88%)
Frame = -1 / -3
Query: 292 RDMMCLWDQFQKTKRGTCCKE*QKSCEWCFMLRWLL 185
RDMMCLWD+FQKTKRGTCCKE*QK + CF+ RWL+
Sbjct: 657 RDMMCLWDKFQKTKRGTCCKE*QKYWK*CFIFRWLV 550
Score = 72.5 bits (152), Expect(5) = 3e-028
Identities = 29/37 (78%), Positives = 32/37 (86%)
Frame = +3 / +2
Query: 183 NSSHRNIKHHSHDFCYSLQHVPRFVF*N*SQRHIISL 293
+++HR IKHH FCYSLQHVPRFVF*N SQRHIISL
Sbjct: 548 STNHRKIKHHFQYFCYSLQHVPRFVF*NLSQRHIISL 658
Score = 36.3 bits (73), Expect(5) = 3e-028
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1 / +1
Query: 133 VYIKNVLA*RCLFKQRP 183
VYIKNVLA*RCLF Q P
Sbjct: 511 VYIKNVLA*RCLFNQPP 561
Score = 36.3 bits (73), Expect(5) = 3e-028
Identities = 15/21 (71%), Positives = 19/21 (90%)
Frame = +3 / +1
Query: 15 MP*FDLKLFKLQTKQT*RYPK 77
+P*FDLKLF+L+TKQT*+ K
Sbjct: 400 IP*FDLKLFRLRTKQT*KISK 462
Score = 34.1 bits (68), Expect(5) = 4e-027
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -3 / -1
Query: 182 GRCLNRHLHAKTFLMYT 132
G LNRH HAKTFL+YT
Sbjct: 560 GGWLNRHRHAKTFLIYT 510
Score = 30.9 bits (61), Expect(5) = 4e-027
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = -1 / -1
Query: 61 VCFVCSLKSFKSNYGM 14
VCFV SLKSFKSN G+
Sbjct: 446 VCFVRSLKSFKSN*GI 399
Score = 28.6 bits (56), Expect(5) = 4e-027
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -3 / -3
Query: 140 MYTALPKRILKELE 99
+YTALPKRIL E+E
Sbjct: 516 IYTALPKRILAEVE 475
Score = 27.6 bits (54), Expect(5) = 3e-028
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1 / +2
Query: 100 SSSFRILLGRAVYI 141
S+S RILLGRAVYI
Sbjct: 476 STSARILLGRAVYI 517
Score = 24.9 bits (48), Expect(5) = 3e-028
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +1 / +3
Query: 61 LEDIQKIVCLP 93
LEDIQ VCLP
Sbjct: 447 LEDIQNFVCLP 479
Score = 23.1 bits (44), Expect(5) = 4e-027
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -3 / -2
Query: 92 GKQTIFWISS 63
G+QT FWISS
Sbjct: 478 GRQTKFWISS 449
>gb|CW318924.1|CW318924 104_811_11474214_148_35883_025 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11474214, DNA
sequence
Length = 696
Score = 118 bits (253), Expect = 6e-026
Identities = 42/62 (67%), Positives = 54/62 (87%)
Frame = -1 / +3
Query: 451 SGELKMFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGYVIAHYQEPRDMMCLW 272
+G L+MFKLEDVAMGIW+N+MKKDGL VKYE D RI ++GC DGYV+AHYQE R ++C+W
Sbjct: 501 NGFLQMFKLEDVAMGIWVNDMKKDGLPVKYETDKRINIDGCNDGYVVAHYQETRHLLCMW 680
Query: 271 DQ 266
++
Sbjct: 681 EK 686
>gb|CF757196.1|CF757196 DSAF1_14_H08.g1_A011 Drought-stressed after flowering Sorghum
bicolor cDNA clone DSAF1_14_H08_A011 3', mRNA sequence
Length = 557
Score = 114 bits (243), Expect = 1e-024
Identities = 45/88 (51%), Positives = 58/88 (65%)
Frame = -1 / +2
Query: 496 IVSKDIAKEVYRKHKSGELKMFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGY 317
I+S+DIAK V R H+ L++FKLEDVAMGIWI + K G V D R EGC+ Y
Sbjct: 2 IISRDIAKFVVRGHQELTLQLFKLEDVAMGIWIQQYKNSGQQVNIVTDDRFYNEGCDADY 181
Query: 316 VIAHYQEPRDMMCLWDQFQKTKRGTCCK 233
V+AHYQ PR MMCLW++ + + CC+
Sbjct: 182 VLAHYQTPRLMMCLWEKLKTEYQAICCE 265
>gb|CF758925.1|CF758925 DSAF1_38_B12.g1_A011 Drought-stressed after flowering Sorghum
bicolor cDNA clone DSAF1_38_B12_A011 3', mRNA sequence
Length = 558
Score = 114 bits (243), Expect = 1e-024
Identities = 45/88 (51%), Positives = 58/88 (65%)
Frame = -1 / +2
Query: 496 IVSKDIAKEVYRKHKSGELKMFKLEDVAMGIWINEMKKDGLDVKYENDGRILVEGCEDGY 317
I+S+DIAK V R H+ L++FKLEDVAMGIWI + K G V D R EGC+ Y
Sbjct: 2 IISRDIAKFVVRGHQELTLQLFKLEDVAMGIWIQQYKNSGQQVNIVTDDRFYNEGCDADY 181
Query: 316 VIAHYQEPRDMMCLWDQFQKTKRGTCCK 233
V+AHYQ PR MMCLW++ + + CC+
Sbjct: 182 VLAHYQTPRLMMCLWEKLKTEYQAICCE 265
>gb|BM327323.1|BM327323 PIC1_12_A11.g1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 473
Score = 54.7 bits (113), Expect(5) = 1e-023
Identities = 20/28 (71%), Positives = 24/28 (85%)
Frame = +3 / -2
Query: 183 NSSHRNIKHHSHDFCYSLQHVPRFVF*N 266
+++HR IKHH FCYSLQHVPRFVF*+
Sbjct: 85 STNHRKIKHHFQYFCYSLQHVPRFVF*D 2
Score = 36.3 bits (73), Expect(5) = 1e-023
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1 / -1
Query: 133 VYIKNVLA*RCLFKQRP 183
VYIKNVLA*RCLF Q P
Sbjct: 122 VYIKNVLA*RCLFNQPP 72
Score = 36.3 bits (73), Expect(5) = 1e-023
Identities = 15/21 (71%), Positives = 19/21 (90%)
Frame = +3 / -1
Query: 15 MP*FDLKLFKLQTKQT*RYPK 77
+P*FDLKLF+L+TKQT*+ K
Sbjct: 233 IP*FDLKLFRLRTKQT*KISK 171
Score = 27.6 bits (54), Expect(5) = 1e-023
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = +1 / -2
Query: 100 SSSFRILLGRAVYI 141
S+S RILLGRAVYI
Sbjct: 157 STSARILLGRAVYI 116
Score = 24.9 bits (48), Expect(5) = 1e-023
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +1 / -3
Query: 61 LEDIQKIVCLP 93
LEDIQ VCLP
Sbjct: 186 LEDIQNFVCLP 154
>gb|CF072395.1|CF072395 FE1_2_F08.g1_A002 Iron-deficient seedlings Sorghum bicolor cDNA
clone FE1_2_F08_A002 5', mRNA sequence
Length = 658
Score = 103 bits (220), Expect = 2e-021
Identities = 41/97 (42%), Positives = 59/97 (60%)
Frame = -1 / -3
Query: 514 AHGPGYIVSKDIAKEVYRKHKSGELKMFKLEDVAMGIWINEMKKDGLDVKYENDGRILVE 335
A+GPGYI+S DIA+ + + + L++FK+EDV+MG+W+ + V+Y +D R
Sbjct: 656 ANGPGYIISSDIAQYILSEFDNKTLRLFKMEDVSMGMWVEKFNTTRSPVEYLHDLRFYQP 477
Query: 334 GCEDGYVIAHYQEPRDMMCLWDQFQKTKRGTCCKE*Q 224
GC DGY AHYQ P+ M+CLW + Q C *Q
Sbjct: 476 GCFDGYFTAHYQSPQHMICLWRKLQAGSTQCCNVR*Q 366
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 718,199,911
Number of Sequences: 832831
Number of extensions: 9595110
Number of successful extensions: 79013
Number of sequences better than 1.0e-020: 13
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 16511218794
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)