TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCF7a10.yg.2.1
(523 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN135280.1|CN135280 OX1_31_F02.g1_A002 Oxidatively-stres... 204 7e-063
gb|CD233682.1|CD233682 SS1_3_B09.g1_A012 Salt-stressed seed... 156 2e-055
gb|CD228436.1|CD228436 CCC1_7_D11.g1_A007 Callus culture/ce... 201 7e-051
gb|CW109339.1|CW109339 104_481_11098049_148_34501_071 Sorgh... 81 4e-041
gb|BE597085.1|BE597085 PI1_70_E04.g1_A002 Pathogen induced ... 85 6e-040
gb|AW287265.2|AW287265 LG1_268_G06.b1_A002 Light Grown 1 (L... 109 4e-037
gb|CL190299.1|CL190299 104_408_10905986_116_32502_009 Sorgh... 75 8e-028
gb|BG463224.1|BG463224 EM1_48_A03.b1_A002 Embryo 1 (EM1) So... 113 2e-024
gb|CW387865.1|CW387865 fsbb001f072g08f0 Sorghum methylation... 85 7e-022
gb|BG739562.1|BG739562 EM1_82_D08.b1_A002 Embryo 1 (EM1) So... 90 2e-021
>gb|CN135280.1|CN135280 OX1_31_F02.g1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_31_F02_A002 5', mRNA sequence
Length = 787
Score = 204 bits (439), Expect(2) = 7e-063
Identities = 86/113 (76%), Positives = 93/113 (82%)
Frame = +3 / +1
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D+RRKP GKI A LT LCI LKQSP F G SVFSR E GVTHVLVTGGAGYIGSH
Sbjct: 379 DSRRKPNFTGKIAVAAALTVLCIIVLKQSPSFSGTSVFSRRETGVTHVLVTGGAGYIGSH 558
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
A LRLL D+YRVTIVDNLSRGNMGAV+VLQ LFP+PGRLQFI+ADLGD +V+
Sbjct: 559 ATLRLLSDNYRVTIVDNLSRGNMGAVRVLQRLFPEPGRLQFIYADLGDAXAVN 717
Score = 150 bits (322), Expect(2) = 2e-044
Identities = 67/108 (62%), Positives = 76/108 (70%)
Frame = -1 / -3
Query: 328 SPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAPPVTK 149
SP+SA +N SLPG GN RT TAP+FPLD+LSTMVTR*LS + + ACEPI PAPPVT
Sbjct: 701 SPKSA*IN*SLPGSGNSR*RTLTAPMFPLDKLSTMVTR*LSLRRRRVACEPIYPAPPVTN 522
Query: 148 TWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFPTLRGFRLAS 5
TWVTP S RENTL+P K GDCF + + V A IFP GFR S
Sbjct: 521 TWVTPVSRRENTLVPLKLGDCFNTIMHNTVSAAATAIFPVKLGFRRES 378
Score = 57.9 bits (120), Expect(2) = 7e-063
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = +1 / +1
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGES 510
A VNKIF+ENAFDAVMHFAA AYVGES
Sbjct: 703 AXAVNKIFSENAFDAVMHFAAVAYVGES 786
Score = 49.6 bits (102), Expect(2) = 2e-044
Identities = 22/25 (88%), Positives = 22/25 (88%)
Frame = -3 / -3
Query: 509 LSPT*AXAAKCITASNAFSANILLT 435
LSPT*A AAKCITASNAFS NIL T
Sbjct: 785 LSPT*ATAAKCITASNAFSENILFT 711
>gb|CD233682.1|CD233682 SS1_3_B09.g1_A012 Salt-stressed seedlings Sorghum bicolor cDNA
clone SS1_3_B09_A012 5', mRNA sequence
Length = 671
Score = 156 bits (335), Expect(3) = 2e-055
Identities = 64/78 (82%), Positives = 74/78 (94%)
Frame = +3 / +1
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQ 290
++FS HEPGVTHVLVTGGAGYIGSHAALRLL+DS+RVTIVDNLSRGN+GA+KVLQ LFP+
Sbjct: 310 TLFSIHEPGVTHVLVTGGAGYIGSHAALRLLKDSFRVTIVDNLSRGNIGAIKVLQNLFPE 489
Query: 291 PGRLQFIFADLGDQKSVS 344
PGRLQFI ADLGD ++V+
Sbjct: 490 PGRLQFIQADLGDPEAVN 543
Score = 112 bits (240), Expect(2) = 5e-031
Identities = 48/71 (67%), Positives = 54/71 (76%)
Frame = -1 / -1
Query: 328 SPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAPPVTK 149
SP+SA MNC+ PG GN+ RT APIFPL+RLSTMVT S +AACEP+*PAPPVT
Sbjct: 527 SPKSACMNCNRPGSGNRFWRTLIAPIFPLERLSTMVTLKESLSRRRAACEPM*PAPPVTS 348
Query: 148 TWVTPGSWREN 116
T VTPGSW EN
Sbjct: 347 TCVTPGSWMEN 315
Score = 66.1 bits (138), Expect(3) = 2e-055
Identities = 27/29 (93%), Positives = 28/29 (96%)
Frame = +1 / +1
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VN+IFAENAFDAVMHFAA AYVGESTLEP
Sbjct: 538 VNRIFAENAFDAVMHFAAVAYVGESTLEP 624
Score = 42.3 bits (86), Expect(2) = 5e-031
Identities = 15/28 (53%), Positives = 22/28 (78%)
Frame = -2 / -3
Query: 519 FQCALSHISXSCKVHHSIKCIFSKYLVD 436
F+ AL++I SCKVH+SIKC+ KY ++
Sbjct: 621 FKRALTNIGHSCKVHNSIKCVLCKYSIN 538
Score = 33.1 bits (66), Expect(3) = 2e-055
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +3 / +1
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +RKPR + K++ +L +C+ L Q P
Sbjct: 193 DPKRKPRCLSKVVMLALLAAMCVVMLTQPP 282
>gb|CD228436.1|CD228436 CCC1_7_D11.g1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_7_D11_A007 5', mRNA sequence
Length = 655
Score = 201 bits (433), Expect = 7e-051
Identities = 84/108 (77%), Positives = 90/108 (83%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D+RRKP GKI A LT +CI LKQSP F G SVFSR E GVTHVLVTGGAGYIGSH
Sbjct: 332 DSRRKPNFTGKIAVAAALTVMCIIVLKQSPSFSGTSVFSRRETGVTHVLVTGGAGYIGSH 511
Query: 186 AALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGD 329
A LRLL D+YRVTIVDNLSRGNMGAV+VLQ LFP+PGRLQFI+ADLGD
Sbjct: 512 ATLRLLSDNYRVTIVDNLSRGNMGAVRVLQRLFPEPGRLQFIYADLGD 655
Score = 149 bits (319), Expect = 4e-035
Identities = 67/108 (62%), Positives = 75/108 (69%)
Frame = -1 / -2
Query: 328 SPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAPPVTK 149
SP+SA +N SLPG GN RT TAP+FPLD+LSTMVTR*LS + + ACEPI PAPPVT
Sbjct: 654 SPKSA*IN*SLPGSGNSR*RTLTAPMFPLDKLSTMVTR*LSLRRRRVACEPIYPAPPVTN 475
Query: 148 TWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFPTLRGFRLAS 5
TWVTP S RENTL+P K GDCF + V A IFP GFR S
Sbjct: 474 TWVTPVSRRENTLVPLKLGDCFNTIMHITVSAAATAIFPVKLGFRRES 331
>gb|CW109339.1|CW109339 104_481_11098049_148_34501_071 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11098049, DNA
sequence
Length = 575
Score = 80.8 bits (170), Expect(3) = 4e-041
Identities = 33/37 (89%), Positives = 36/37 (97%)
Frame = +3 / +1
Query: 120 SRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIV 230
S HEPGVTHVLVTGGAGYIGSHAALRLL+DS+RVTI+
Sbjct: 1 SIHEPGVTHVLVTGGAGYIGSHAALRLLKDSFRVTIM 111
Score = 74.8 bits (157), Expect(3) = 4e-041
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = +3 / +1
Query: 231 DNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
DNLSRGN+GA+KVLQ LFP+PGRLQFI ADLGD ++VS
Sbjct: 250 DNLSRGNIGAIKVLQNLFPEPGRLQFIQADLGDPEAVS 363
Score = 58.3 bits (121), Expect(3) = 3e-026
Identities = 24/40 (60%), Positives = 30/40 (75%)
Frame = -1 / -1
Query: 349 DEDTDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLS 230
++DT SP+SA MNC+ PG GN+ RT APIFPL+RLS
Sbjct: 368 NQDTASGSPKSACMNCNRPGSGNRFWRTLIAPIFPLERLS 249
Score = 54.2 bits (112), Expect(3) = 3e-026
Identities = 23/34 (67%), Positives = 25/34 (73%)
Frame = -1 / -1
Query: 226 MVTR*LSCKSLKAACEPI*PAPPVTKTWVTPGSW 125
MVT S +AACEP+*PAPPVT T VTPGSW
Sbjct: 107 MVTLKESLSRRRAACEPM*PAPPVTSTCVTPGSW 6
Score = 52.4 bits (108), Expect(3) = 4e-041
Identities = 21/24 (87%), Positives = 23/24 (95%)
Frame = +1 / +3
Query: 427 ASQVNKIFAENAFDAVMHFAAXAY 498
+SQVN+IFAENAFDAVMHFAA AY
Sbjct: 504 SSQVNRIFAENAFDAVMHFAAVAY 575
Score = 45.1 bits (92), Expect(3) = 3e-026
Identities = 21/23 (91%), Positives = 21/23 (91%)
Frame = -3 / -2
Query: 497 *AXAAKCITASNAFSANILLTWE 429
*A AAKCITASNAFSANILLT E
Sbjct: 574 *ATAAKCITASNAFSANILLT*E 506
>gb|BE597085.1|BE597085 PI1_70_E04.g1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA,
mRNA sequence
Length = 623
Score = 84.9 bits (179), Expect(3) = 6e-040
Identities = 35/38 (92%), Positives = 37/38 (97%)
Frame = +3 / -2
Query: 117 FSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIV 230
FS HEPGVTHVLVTGGAGYIGSHAALRLL+DS+RVTIV
Sbjct: 574 FSIHEPGVTHVLVTGGAGYIGSHAALRLLKDSFRVTIV 461
Score = 74.8 bits (157), Expect(3) = 6e-040
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = +3 / -2
Query: 231 DNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
DNLSRGN+GA+KVLQ LFP+PGRLQFI ADLGD ++VS
Sbjct: 322 DNLSRGNIGAIKVLQNLFPEPGRLQFIQADLGDPEAVS 209
Score = 61.6 bits (128), Expect(3) = 2e-027
Identities = 26/39 (66%), Positives = 29/39 (74%)
Frame = -1 / +3
Query: 232 STMVTR*LSCKSLKAACEPI*PAPPVTKTWVTPGSWREN 116
+TMVT S +AACEP+*PAPPVT T VTPGSW EN
Sbjct: 459 TTMVTLKESLSRRRAACEPM*PAPPVTSTCVTPGSWMEN 575
Score = 58.3 bits (121), Expect(3) = 2e-027
Identities = 24/40 (60%), Positives = 30/40 (75%)
Frame = -1 / +3
Query: 349 DEDTDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLS 230
++DT SP+SA MNC+ PG GN+ RT APIFPL+RLS
Sbjct: 204 NQDTASGSPKSACMNCNRPGSGNRFWRTLIAPIFPLERLS 323
Score = 44.6 bits (91), Expect(3) = 6e-040
Identities = 18/21 (85%), Positives = 20/21 (95%)
Frame = +1 / -1
Query: 427 ASQVNKIFAENAFDAVMHFAA 489
+SQVN+IFAENAFDA MHFAA
Sbjct: 68 SSQVNRIFAENAFDAGMHFAA 6
Score = 41.8 bits (85), Expect(3) = 2e-027
Identities = 19/22 (86%), Positives = 19/22 (86%)
Frame = -3 / +1
Query: 494 AXAAKCITASNAFSANILLTWE 429
A AAKCI ASNAFSANILLT E
Sbjct: 1 ATAAKCIPASNAFSANILLT*E 66
>gb|AW287265.2|AW287265 LG1_268_G06.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 501
Score = 109 bits (233), Expect(2) = 4e-037
Identities = 45/56 (80%), Positives = 53/56 (94%)
Frame = +3 / +3
Query: 177 GSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
GSHAALRLL+DS+RVTIVDNLSRGN+GA+KVLQ LFP+PGRLQFI ADLGD ++V+
Sbjct: 9 GSHAALRLLKDSFRVTIVDNLSRGNIGAIKVLQNLFPEPGRLQFIQADLGDPEAVN 176
Score = 66.1 bits (138), Expect(2) = 4e-037
Identities = 27/29 (93%), Positives = 28/29 (96%)
Frame = +1 / +3
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VN+IFAENAFDAVMHFAA AYVGESTLEP
Sbjct: 171 VNRIFAENAFDAVMHFAAVAYVGESTLEP 257
>gb|CL190299.1|CL190299 104_408_10905986_116_32502_009 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10905986, DNA
sequence
Length = 633
Score = 74.8 bits (157), Expect(2) = 8e-028
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = +3 / +3
Query: 231 DNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
DNLSRGN+GA+KVLQ LFP+PGRLQFI ADLGD ++VS
Sbjct: 93 DNLSRGNIGAIKVLQNLFPEPGRLQFIQADLGDPEAVS 206
Score = 70.7 bits (148), Expect(2) = 8e-028
Identities = 29/32 (90%), Positives = 31/32 (96%)
Frame = +1 / +2
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
+SQVN+IFAENAFDAVMHFAA AYVGESTLEP
Sbjct: 347 SSQVNRIFAENAFDAVMHFAAVAYVGESTLEP 442
>gb|BG463224.1|BG463224 EM1_48_A03.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 518
Score = 113 bits (242), Expect = 2e-024
Identities = 48/68 (70%), Positives = 50/68 (73%)
Frame = +3 / +1
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGSH 185
D+RRKP GKI A LT +CI LKQSP F G SVFSR E GVTHVL TGGAGYIGSH
Sbjct: 313 DSRRKPNFTGKIAVAAALTVMCIIVLKQSPSFSGTSVFSRRETGVTHVLRTGGAGYIGSH 492
Query: 186 AALRLLQD 209
A LRLL D
Sbjct: 493 ATLRLLSD 516
>gb|CW387865.1|CW387865 fsbb001f072g08f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f072g08, DNA
sequence
Length = 446
Score = 84.9 bits (179), Expect(2) = 7e-022
Identities = 35/38 (92%), Positives = 37/38 (97%)
Frame = +3 / -1
Query: 117 FSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIV 230
FS HEPGVTHVLVTGGAGYIGSHAALRLL+DS+RVTIV
Sbjct: 314 FSIHEPGVTHVLVTGGAGYIGSHAALRLLKDSFRVTIV 201
Score = 39.1 bits (79), Expect(2) = 7e-022
Identities = 14/18 (77%), Positives = 17/18 (94%)
Frame = +3 / -3
Query: 240 SRGNMGAVKVLQGLFPQP 293
SRGN+GA+KVLQ LFP+P
Sbjct: 54 SRGNIGAIKVLQNLFPEP 1
>gb|BG739562.1|BG739562 EM1_82_D08.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 521
Score = 89.5 bits (189), Expect(2) = 2e-021
Identities = 37/42 (88%), Positives = 40/42 (95%)
Frame = +3 / +3
Query: 114 VFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNL 239
+FS HEPGVTHVLVTGGAGYIGSHAALRLL+DS+RVTIVD L
Sbjct: 396 LFSIHEPGVTHVLVTGGAGYIGSHAALRLLKDSFRVTIVDXL 521
Score = 33.1 bits (66), Expect(2) = 2e-021
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +RKPR + K++ +L +C+ L Q P
Sbjct: 272 DPKRKPRCLSKVVMLALLAAMCVVMLTQPP 361
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 665,943,966
Number of Sequences: 832831
Number of extensions: 9881913
Number of successful extensions: 101149
Number of sequences better than 1.0e-020: 13
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 14357581560
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)