TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.068P22F020924.3.1
(714 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE600856.1|BE600856 PI1_92_B01.b1_A002 Pathogen induced ... 222 5e-057
gb|CF759670.1|CF759670 DSAF1_48_C06.b1_A011 Drought-stresse... 201 1e-050
gb|CN125223.1|CN125223 RHOH1_9_F11.g1_A002 Acid- and alkali... 194 1e-048
gb|BE361313.1|BE361313 DG1_71_D11.b1_A002 Dark Grown 1 (DG1... 100 2e-037
gb|CW027767.1|CW027767 104_254_10498697_114_30395 Sorghum m... 118 4e-034
gb|CL151510.1|CL151510 104_334_10779304_114_31361_088 Sorgh... 100 3e-031
gb|CD229872.1|CD229872 CCC1_20_A05.g1_A007 Callus culture/c... 133 3e-030
gb|CN126543.1|CN126543 RHOH1_17_F12.g1_A002 Acid- and alkal... 132 5e-030
gb|CN134785.1|CN134785 OX1_28_B01.g1_A002 Oxidatively-stres... 110 3e-023
gb|CN129158.1|CN129158 RHOH1_33_G09.g1_A002 Acid- and alkal... 108 1e-022
gb|CW350751.1|CW350751 fsbb001f012d11k0 Sorghum methylation... 100 3e-022
gb|BI076089.1|BI076089 IP1_25_E12.b1_A002 Immature pannicle... 79 5e-022
gb|BG947821.1|BG947821 IP1_5_E12.b1_A002 Immature pannicle ... 79 2e-021
>gb|BE600856.1|BE600856 PI1_92_B01.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA,
mRNA sequence
Length = 537
Score = 222 bits (479), Expect = 5e-057
Identities = 94/132 (71%), Positives = 110/132 (83%)
Frame = +2 / +2
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQS 496
DQKT+RRLAQNREAARKSRLRKKAYVQQLE+S+LKL GIFISSS DQ+
Sbjct: 119 DQKTMRRLAQNREAARKSRLRKKAYVQQLESSKLKLAQLEQELQKARQQGIFISSSGDQT 298
Query: 497 HSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLK 676
H+MSGNGA FD+EYARWLE+ N+QI+ELR V+AHASD+DLR +VD IM+HYDEIF++K
Sbjct: 299 HAMSGNGALTFDIEYARWLEDQNKQINELRTAVNAHASDSDLRLIVDGIMAHYDEIFKVK 478
Query: 677 GNAAKADVFHVL 712
G AAKADVFH+L
Sbjct: 479 GVAAKADVFHIL 514
>gb|CF759670.1|CF759670 DSAF1_48_C06.b1_A011 Drought-stressed after flowering Sorghum
bicolor cDNA clone DSAF1_48_C06_A011 5', mRNA sequence
Length = 569
Score = 201 bits (433), Expect = 1e-050
Identities = 85/122 (69%), Positives = 100/122 (81%)
Frame = +2 / +1
Query: 347 NREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQSHSMSGNGASA 526
NREAARKSRLRKKAYVQQLE+S+LKL GIFISSS DQ+H+MSGNGA
Sbjct: 1 NREAARKSRLRKKAYVQQLESSKLKLAQLEQELQKARQQGIFISSSGDQTHAMSGNGALT 180
Query: 527 FDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAKADVFH 706
FD+EYARWLE+ N+QI+ELR V+AHASD+DLR +VD IM+HYDEIF++KG AAKADVFH
Sbjct: 181 FDIEYARWLEDQNKQINELRTAVNAHASDSDLRLIVDGIMAHYDEIFKVKGVAAKADVFH 360
Query: 707 VL 712
+L
Sbjct: 361 IL 366
>gb|CN125223.1|CN125223 RHOH1_9_F11.g1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_9_F11_A002 5', mRNA sequence
Length = 776
Score = 194 bits (418), Expect = 1e-048
Identities = 83/117 (70%), Positives = 92/117 (78%)
Frame = +2 / +1
Query: 362 RKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQSHSMSGNGASAFDMEY 541
RKSRLRKKAY+Q LE+SRLKLT GIFIS+S DQ S SGNGA AFDMEY
Sbjct: 205 RKSRLRKKAYIQNLESSRLKLTQLEQELQRARQQGIFISTSGDQPQSTSGNGALAFDMEY 384
Query: 542 ARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAKADVFHVL 712
ARWLEEHN+ ++ELRA V+AHA D DLR +VD IM+HYDEIFRLKG AAKADVFHVL
Sbjct: 385 ARWLEEHNKHVNELRAAVNAHAGDNDLRGIVDSIMAHYDEIFRLKGVAAKADVFHVL 555
Score = 104 bits (222), Expect = 1e-021
Identities = 50/88 (56%), Positives = 59/88 (67%)
Frame = -1 / -1
Query: 714 DKT*KTSALAALPLSLKISS*CDMILSTTLRRXXXXXXXXXXXXXXLICRLCSSNHLAYS 535
D T*KTSALAA PLSLKISS*C ++LST RR C LCSSN AYS
Sbjct: 557 DST*KTSALAATPLSLKISS*CAIMLSTIPRRSLSPACALTAALNSFTCLLCSSNQRAYS 378
Query: 534 ISKADAPFPLMECD*STELDIKMPC*RA 451
+S A APFPL++ *S +++IK+PC*RA
Sbjct: 377 MSNAKAPFPLVD*G*SPDVEIKIPC*RA 294
Score = 103 bits (220), Expect = 3e-021
Identities = 55/114 (48%), Positives = 71/114 (62%)
Frame = +3 / +2
Query: 363 GRAD*GRRHMFNSWRIAG*NLPN*SKSCKELVSRAFLYLVQLISHTP*VEMGHRPLIWSM 542
G+A RRH + R+A *NL +*SKS LV+R FL+ Q I+ VEM L WSM
Sbjct: 206 GKAVYERRHTSKTLRVAD*NLLS*SKSFSGLVNRVFLFRHQEINLNQQVEMELWHLTWSM 385
Query: 543 RGGWKSTIGKLVN*GLVSVLMQVILIFEVLLIRSCHTMMRFLGSKAMQPRQMFF 704
GW+ST ++*GL+S+ M VI+IF VLLI HT +F GS+ Q +QMFF
Sbjct: 386 HAGWRSTTNM*MS*GLLSMHMPVIMIFVVLLIALWHTTTKFSGSRVWQLKQMFF 547
>gb|BE361313.1|BE361313 DG1_71_D11.b1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 555
Score = 99.6 bits (211), Expect(2) = 2e-037
Identities = 35/69 (50%), Positives = 55/69 (79%)
Frame = +2 / +1
Query: 506 SGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNA 685
S +GA+ FD+EYARWL++H R+++EL + AH D DLR++VD ++H+DE+F+LK +A
Sbjct: 325 SSSGAAMFDVEYARWLDDHGRRMAELHGALHAHLPDGDLRAIVDDTLTHHDELFQLKASA 504
Query: 686 AKADVFHVL 712
AK+DV H++
Sbjct: 505 AKSDVVHLI 531
Score = 78.0 bits (164), Expect(2) = 2e-037
Identities = 34/56 (60%), Positives = 40/56 (71%)
Frame = +2 / +1
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSS 484
D KT+RRLAQNREAARKSRLRKKAY+QQLE+S+LKL GIF+ +
Sbjct: 145 DPKTIRRLAQNREAARKSRLRKKAYIQQLESSKLKLAQIEQDMQRAHSQGIFLGGA 312
>gb|CW027767.1|CW027767 104_254_10498697_114_30395 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10498697, DNA
sequence
Length = 816
Score = 118 bits (253), Expect(3) = 4e-034
Identities = 46/66 (69%), Positives = 57/66 (86%)
Frame = +2 / -2
Query: 515 GASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAKA 694
GA FD+EY+RW E+ N+Q++ELR V+AHASD+DLR +VD IM+HYDEIFRLKG AAKA
Sbjct: 278 GAMTFDLEYSRWQEDQNKQMNELRTAVNAHASDSDLRLIVDGIMAHYDEIFRLKGIAAKA 99
Query: 695 DVFHVL 712
DVFH+L
Sbjct: 98 DVFHIL 81
Score = 38.2 bits (77), Expect(3) = 4e-034
Identities = 15/18 (83%), Positives = 17/18 (94%)
Frame = +2 / -2
Query: 464 GIFISSSVDQSHSMSGNG 517
GIFISSS DQ+H+MSGNG
Sbjct: 458 GIFISSSGDQTHAMSGNG 405
Score = 30.4 bits (60), Expect(3) = 4e-034
Identities = 13/17 (76%), Positives = 15/17 (88%)
Frame = +2 / -2
Query: 374 LRKKAYVQQLENSRLKL 424
L KAYVQQLE+S+LKL
Sbjct: 638 LFSKAYVQQLESSKLKL 588
>gb|CL151510.1|CL151510 104_334_10779304_114_31361_088 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10779304, DNA
sequence
Length = 749
Score = 100 bits (213), Expect(3) = 3e-031
Identities = 35/66 (53%), Positives = 54/66 (81%)
Frame = +2 / -2
Query: 515 GASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAKA 694
GA+ FD+EYARWL++H R+++EL + AH D DLR++VD ++H+DE+F+LK +AAK+
Sbjct: 256 GAAMFDVEYARWLDDHGRRMAELHGALHAHLPDGDLRAIVDDTLTHHDELFQLKASAAKS 77
Query: 695 DVFHVL 712
DVFH++
Sbjct: 76 DVFHLI 59
Score = 49.2 bits (101), Expect(3) = 3e-031
Identities = 20/26 (76%), Positives = 24/26 (92%)
Frame = +2 / -1
Query: 320 QKTLRRLAQNREAARKSRLRKKAYVQ 397
Q+T+RRLAQNREAARKSRLRKK ++
Sbjct: 734 QQTIRRLAQNREAARKSRLRKKVNIE 657
Score = 27.6 bits (54), Expect(3) = 3e-031
Identities = 10/14 (71%), Positives = 14/14 (100%)
Frame = +2 / -2
Query: 383 KAYVQQLENSRLKL 424
+AY+QQLE+S+LKL
Sbjct: 580 QAYIQQLESSKLKL 539
>gb|CD229872.1|CD229872 CCC1_20_A05.g1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_20_A05_A007 5', mRNA sequence
Length = 612
Score = 133 bits (285), Expect = 3e-030
Identities = 57/84 (67%), Positives = 65/84 (77%)
Frame = +2 / +2
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQS 496
DQK LRRLAQNREAARKSRLRKKAYVQQLE+S+LKL GIFISSS DQ+
Sbjct: 359 DQKVLRRLAQNREAARKSRLRKKAYVQQLESSKLKLASLEQELQKARQQGIFISSSGDQT 538
Query: 497 HSMSGNGASAFDMEYARWLEEHNR 568
H+MSGNGA FD+EY+RW E+ N+
Sbjct: 539 HAMSGNGAMTFDLEYSRWQEDQNK 610
>gb|CN126543.1|CN126543 RHOH1_17_F12.g1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_17_F12_A002 5', mRNA sequence
Length = 833
Score = 132 bits (283), Expect = 5e-030
Identities = 57/81 (70%), Positives = 65/81 (80%)
Frame = +2 / +1
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQS 496
DQKT+RRLAQNREAAR+SRLRKKAYVQQLE+S+LKL GIFISSS DQ+
Sbjct: 586 DQKTMRRLAQNREAARRSRLRKKAYVQQLESSKLKLAQLEQELQKARQQGIFISSSGDQT 765
Query: 497 HSMSGNGASAFDMEYARWLEE 559
H+MSGNGA FD+EYARWLE+
Sbjct: 766 HAMSGNGALTFDIEYARWLED 828
>gb|CN134785.1|CN134785 OX1_28_B01.g1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_28_B01_A002 5', mRNA sequence
Length = 718
Score = 110 bits (234), Expect = 3e-023
Identities = 49/72 (68%), Positives = 55/72 (76%)
Frame = +2 / +3
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQS 496
DQ+T+RRLAQNREAARKSRLRKKAYVQQLE+S+LKL GIFIS S DQ+
Sbjct: 501 DQETMRRLAQNREAARKSRLRKKAYVQQLESSKLKLAQLEQELQKARQQGIFISGSGDQT 680
Query: 497 HSMSGNGASAFD 532
H+MSGNGA FD
Sbjct: 681 HAMSGNGALTFD 716
>gb|CN129158.1|CN129158 RHOH1_33_G09.g1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_33_G09_A002 5', mRNA sequence
Length = 801
Score = 108 bits (230), Expect = 1e-022
Identities = 49/68 (72%), Positives = 53/68 (77%)
Frame = +2 / +3
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQS 496
DQK LRRLAQNREAARKSRLRKKAYVQQLE+S+LKL GIFISSS DQ+
Sbjct: 303 DQKVLRRLAQNREAARKSRLRKKAYVQQLESSKLKLASLEQELQKARQQGIFISSSGDQT 482
Query: 497 HSMSGNGA 520
H+MSGNGA
Sbjct: 483 HAMSGNGA 506
>gb|CW350751.1|CW350751 fsbb001f012d11k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f012d11, DNA
sequence
Length = 763
Score = 100 bits (213), Expect(2) = 3e-022
Identities = 35/66 (53%), Positives = 54/66 (81%)
Frame = +2 / -2
Query: 515 GASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNAAKA 694
GA+ FD+EYARWL++H R+++EL + AH D DLR++VD ++H+DE+F+LK +AAK+
Sbjct: 366 GAAMFDVEYARWLDDHGRRMAELHGALHAHLPDGDLRAIVDDTLTHHDELFQLKASAAKS 187
Query: 695 DVFHVL 712
DVFH++
Sbjct: 186 DVFHLI 169
Score = 27.6 bits (54), Expect(2) = 3e-022
Identities = 10/14 (71%), Positives = 14/14 (100%)
Frame = +2 / -2
Query: 383 KAYVQQLENSRLKL 424
+AY+QQLE+S+LKL
Sbjct: 690 QAYIQQLESSKLKL 649
>gb|BI076089.1|BI076089 IP1_25_E12.b1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA,
mRNA sequence
Length = 596
Score = 78.5 bits (165), Expect(2) = 5e-022
Identities = 34/56 (60%), Positives = 40/56 (71%)
Frame = +2 / +3
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSS 484
D KTLRRLAQNREAARKSRLRKKAY+QQLE+SR++L G+F +S
Sbjct: 234 DPKTLRRLAQNREAARKSRLRKKAYIQQLESSRIRLAQLEQELHTARAQGVFFPNS 401
Score = 46.9 bits (96), Expect(2) = 5e-022
Identities = 18/40 (45%), Positives = 26/40 (65%)
Frame = +2 / +3
Query: 518 ASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVD 637
A+ FD+EY RW EEH R + ELRA + H + +L+ V+
Sbjct: 474 AAMFDVEYGRWQEEHYRLMYELRAALQQHLPEGELQMYVE 593
>gb|BG947821.1|BG947821 IP1_5_E12.b1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA,
mRNA sequence
Length = 581
Score = 78.5 bits (165), Expect(2) = 2e-021
Identities = 34/56 (60%), Positives = 40/56 (71%)
Frame = +2 / +3
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSS 484
D KTLRRLAQNREAARKSRLRKKAY+QQLE+SR++L G+F +S
Sbjct: 234 DPKTLRRLAQNREAARKSRLRKKAYIQQLESSRIRLAQLEQELHTARAQGVFFPNS 401
Score = 45.1 bits (92), Expect(2) = 2e-021
Identities = 17/36 (47%), Positives = 24/36 (66%)
Frame = +2 / +3
Query: 518 ASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLR 625
A+ FD+EY RW EEH R + ELRA + H + +L+
Sbjct: 474 AAMFDVEYGRWQEEHYRLMYELRAALQQHLPEGELQ 581
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 745,142,229
Number of Sequences: 832831
Number of extensions: 9690945
Number of successful extensions: 70920
Number of sequences better than 1.0e-020: 14
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 21742903806
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)