TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.064G21F020918.3.1
(705 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CD432412.1|CD432412 ETH1_29_A04.g1_A002 Ethylene-treated... 228 7e-098
gb|BE598686.1|BE598686 PI1_81_F04.b1_A002 Pathogen induced ... 232 1e-081
gb|CD209204.1|CD209204 HS1_46_F10.g1_A012 Heat-shocked seed... 149 4e-063
gb|BG464223.1|BG464223 EM1_71_B05.b1_A002 Embryo 1 (EM1) So... 152 6e-036
gb|BG649135.1|BG649135 EM1_56_A05.b1_A002 Embryo 1 (EM1) So... 152 6e-036
gb|CW428236.1|CW428236 fsbb001f140l08k0 Sorghum methylation... 123 2e-031
gb|CW415759.1|CW415759 fsbb001f116a03f0 Sorghum methylation... 118 5e-030
gb|CW108361.1|CW108361 104_480_11097488_116_34496_032 Sorgh... 80 1e-024
>gb|CD432412.1|CD432412 ETH1_29_A04.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA
clone ETH1_29_A04_A002 5', mRNA sequence
Length = 700
Score = 228 bits (493), Expect(3) = 6e-094
Identities = 88/100 (88%), Positives = 90/100 (90%)
Frame = -2 / -1
Query: 704 HCCIWWWCPIRRHHLWTPHIRVPLMGWSYR*MKKSRWGYWSCPSSIIVCLELRPIRICCG 525
HCCIWWWCPIRRHHL TPHI V L WSYR*MKKSRWG+ SCP SIIVCLELRPIRICCG
Sbjct: 694 HCCIWWWCPIRRHHL*TPHIWVSLRAWSYR*MKKSRWGHGSCPRSIIVCLELRPIRICCG 515
Query: 524 GVSRCTRLILYC*RFLRFGPSSHITLSHCLFPSSTLMFLL 405
GVSRCT LILYC*RFLRFGPSSHI+LSHCLF SS LMFLL
Sbjct: 514 GVSRCTLLILYC*RFLRFGPSSHISLSHCLFLSSILMFLL 395
Score = 226 bits (487), Expect(3) = 7e-098
Identities = 90/102 (88%), Positives = 95/102 (93%)
Frame = +1 / +3
Query: 400 QSKRNIRVEDGNKQWERVMWLLGPNLKNRQQYRMSRVHLLTPPQHILIGLSSRHTIMLLG 579
+ KRNIR+E+ NKQWER MWLLGPNLKNRQQYRMSRVHLLTPPQHILIGLSSRHTIMLLG
Sbjct: 390 KGKRNIRIEERNKQWEREMWLLGPNLKNRQQYRMSRVHLLTPPQHILIGLSSRHTIMLLG 569
Query: 580 QLQ*PHRLFFIHL*LQPIRGTRICGVHR*CLLMGHHHHMQQC 705
QL *PHRLFFIHL*LQ +R T ICGV+R*CLLMGHHHHMQQC
Sbjct: 570 QLP*PHRLFFIHL*LQALRDTHICGVYR*CLLMGHHHHMQQC 695
Score = 222 bits (479), Expect(2) = 1e-089
Identities = 93/105 (88%), Positives = 96/105 (91%)
Frame = -1 / -3
Query: 705 TLLHMVVVSHKEASSVDPTYTGAPDGLELQMNEKKPVGLLELSQQHYSMPGTETNQDMLW 526
TLLHMVVVSHKEASSVDPTY G P+GLELQMNEKKPVG ELSQ+HYSMPGTETNQDMLW
Sbjct: 695 TLLHMVVVSHKEASSVDPTYMGVPEGLELQMNEKKPVGSRELSQEHYSMPGTETNQDMLW 516
Query: 525 GG*QVYSAHSVLLTIFEIWT**PHHPFPLFISVLYPYVPFALYSL 391
GG* VYSAHSVLLTIFEIWT**PH PFPLFIS+LYPYVPFA L
Sbjct: 515 GG**VYSAHSVLLTIFEIWT**PHLPFPLFISLLYPYVPFAFILL 381
Score = 214 bits (462), Expect(2) = 5e-087
Identities = 91/104 (87%), Positives = 95/104 (91%)
Frame = +2 / +1
Query: 392 REYKAKGT*G*RTEINNGKG*CGY*VQISKIVSNTE*AEYTC*PPHSIS*LVSVPGIL*C 571
+ KAKGT*G*R EINNGKG CGY*VQISKIVSNTE*AEYT *PPHSIS*LVSVPGIL*C
Sbjct: 382 KRIKAKGT*G*RREINNGKGRCGY*VQISKIVSNTE*AEYTY*PPHSIS*LVSVPGIL*C 561
Query: 572 CWDSSSNPTGFFSFICSSNPSGAPVYVGSTDDASLWDTTTICNN 703
WDSS +PTGFFSFICSS PSG P+YVGSTDDASLWDTTTIC+N
Sbjct: 562 SWDSSRDPTGFFSFICSSKPSGTPIYVGSTDDASLWDTTTICSN 693
Score = 164 bits (352), Expect(2) = 3e-066
Identities = 66/84 (78%), Positives = 68/84 (80%)
Frame = +3 / +2
Query: 402 KQKEHXXXXXXXXXXXXDVATRSKSQKSSAIQNEPSTPANPPTAYPDWSQFQAYYNAAGT 581
+QKEH DVATRSKSQKSSAIQNE STP NPPTAYPDWSQFQAYYNA GT
Sbjct: 392 RQKEHKDRGEK*TMGKGDVATRSKSQKSSAIQNEQSTPTNPPTAYPDWSQFQAYYNAPGT 571
Query: 582 APVTPPAFFHSSVAPTHQGHPYMW 653
APVTPPAFFHSSVAP+ QGHPYMW
Sbjct: 572 APVTPPAFFHSSVAPSPQGHPYMW 643
Score = 149 bits (321), Expect(3) = 7e-098
Identities = 61/71 (85%), Positives = 61/71 (85%)
Frame = +3 / +3
Query: 60 APGIPKSCGASAPTPLVDSSLPNTPEESFSATRLPPLCRAGVEWRSTTTWIGLFFCWLDA 239
AP IPK C ASAPT LVDSSLPNTPEESFS TRLPPLCRAGVEWRS TT IGLFFCWL
Sbjct: 36 APPIPKFCAASAPTLLVDSSLPNTPEESFSETRLPPLCRAGVEWRSITT*IGLFFCWLGT 215
Query: 240 ESVLQCLHRLY 272
ES LQCLHRLY
Sbjct: 216 ESALQCLHRLY 248
Score = 134 bits (288), Expect(2) = 2e-057
Identities = 60/78 (76%), Positives = 61/78 (78%)
Frame = -3 / -2
Query: 637 P*WVGATDE*KKAGGVTGAVPAAL*YAWN*DQXXXXXXXXXXXXXSFCIADDF*DLDLVA 458
P* +GATDE*KKAGGVTGAVP AL*YAWN*DQ SFCIADDF*DLDLVA
Sbjct: 627 P*GLGATDE*KKAGGVTGAVPGAL*YAWN*DQSGYAVGGLVGVLCSFCIADDF*DLDLVA 448
Query: 457 TSPFPIVYFRPLPLCSFC 404
TSPFPIVYF PL LCSFC
Sbjct: 447 TSPFPIVYFSPLSLCSFC 394
Score = 132 bits (283), Expect(3) = 6e-094
Identities = 54/64 (84%), Positives = 56/64 (87%)
Frame = -1 / -2
Query: 315 KKHGNCF*LCNFFRNRACEGIEGQIQRLTSRRRDQSRWWWISTPRQHGREEEDESRRRIP 136
+K GN F CNFFR+RACEGIEGQIQ LTSRRRDQS W WISTPRQ GREEEDESRRRIP
Sbjct: 291 QKRGNSFLNCNFFRDRACEGIEGQIQCLTSRRRDQSTW*WISTPRQPGREEEDESRRRIP 112
Query: 135 PACS 124
PACS
Sbjct: 111 PACS 100
Score = 131 bits (280), Expect(2) = 1e-089
Identities = 57/67 (85%), Positives = 60/67 (89%)
Frame = -3 / -1
Query: 259 RH*RTDSASNQQKKRPIQVVVDLHSTPARQRGGRRVAEKDSSGVFGREESTSGVGAEAPQ 80
RH*R DS NQQKKRPI VV+DLHSTPARQRGGRRV+EKDSSGVFGREESTS VGAEA Q
Sbjct: 235 RH*RADSVPNQQKKRPIYVVMDLHSTPARQRGGRRVSEKDSSGVFGREESTSRVGAEAAQ 56
Query: 79 DLGIPGA 59
+LGI GA
Sbjct: 55 NLGIGGA 35
Score = 128 bits (274), Expect(2) = 5e-087
Identities = 59/69 (85%), Positives = 59/69 (85%)
Frame = +1 / +1
Query: 106 SSTPLFRTRRRNPSPRLVFLLSAVLAWSGDPPPPGLVSSSAG*TLNLSFNAFTGSIPEEV 285
SSTPL RTRRRNPSPRLVFLLSA LAWSGDP P LVSSSAG* LNL FNAFTGSIPEEV
Sbjct: 82 SSTPLCRTRRRNPSPRLVFLLSAGLAWSGDPSPRRLVSSSAG*ALNLPFNAFTGSIPEEV 261
Query: 286 AELEAIAVF 312
A EAIA F
Sbjct: 262 AV*EAIATF 288
Score = 111 bits (237), Expect(2) = 2e-057
Identities = 49/58 (84%), Positives = 51/58 (87%)
Frame = -2 / -3
Query: 275 GIEPVKALKDRFSV*PAEEETNPGGGGSPLHASTAERRKTSRGEGFLRRVRKRGVDER 102
GIEPVKALK RFS *PAEEETN G GSPLHAS AERRKTS GEGFLRRVR+RGVDE+
Sbjct: 251 GIEPVKALKGRFSA*PAEEETNLRGDGSPLHASPAERRKTSLGEGFLRRVRQRGVDEQ 78
Score = 111 bits (237), Expect(2) = 3e-066
Identities = 48/65 (73%), Positives = 50/65 (76%)
Frame = +2 / +2
Query: 68 NSQIXXXXXXXXXXXXXSSEHAGGILLRDSSSSSLPCWRGVEIHHHLDWSLLLLVRR*IC 247
NSQI S+EHAGGILLRDSSSSSLP WRGVEIHHH+DWSLLLLVR *IC
Sbjct: 44 NSQILRRLRPNPARRLLSAEHAGGILLRDSSSSSLPGWRGVEIHHHVDWSLLLLVRH*IC 223
Query: 248 PSMPS 262
PSMPS
Sbjct: 224 PSMPS 238
Score = 24.0 bits (46), Expect(3) = 6e-094
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -3 / -3
Query: 382 KRTAFTCSVSNLD 344
K+ A +CSVSNLD
Sbjct: 353 KQNATSCSVSNLD 315
Score = 22.6 bits (43), Expect(3) = 7e-098
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +1 / +2
Query: 334 FGSYPDWIRNM 366
F +PDW RNM
Sbjct: 305 FLGHPDWTRNM 337
>gb|BE598686.1|BE598686 PI1_81_F04.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA,
mRNA sequence
Length = 558
Score = 232 bits (502), Expect(3) = 1e-081
Identities = 88/100 (88%), Positives = 90/100 (90%)
Frame = -2 / -3
Query: 704 HCCIWWWCPIRRHHLWTPHIRVPLMGWSYR*MKKSRWGYWSCPSSIIVCLELRPIRICCG 525
HCCIWWWCPI RHHLWTPHI V L WSYR*MKKSRWG+ SCP SIIVCLELRPIRICCG
Sbjct: 544 HCCIWWWCPIXRHHLWTPHIWVSLRAWSYR*MKKSRWGHGSCPRSIIVCLELRPIRICCG 365
Query: 524 GVSRCTRLILYC*RFLRFGPSSHITLSHCLFPSSTLMFLL 405
GVSRCT LILYC*RFLRFGPSSHI+LSHCLF SS LMFLL
Sbjct: 364 GVSRCTLLILYC*RFLRFGPSSHISLSHCLFLSSILMFLL 245
Score = 228 bits (493), Expect(3) = 4e-077
Identities = 91/102 (89%), Positives = 95/102 (93%)
Frame = +1 / +3
Query: 400 QSKRNIRVEDGNKQWERVMWLLGPNLKNRQQYRMSRVHLLTPPQHILIGLSSRHTIMLLG 579
+ KRNIR+E+ NKQWER MWLLGPNLKNRQQYRMSRVHLLTPPQHILIGLSSRHTIMLLG
Sbjct: 240 KGKRNIRIEERNKQWEREMWLLGPNLKNRQQYRMSRVHLLTPPQHILIGLSSRHTIMLLG 419
Query: 580 QLQ*PHRLFFIHL*LQPIRGTRICGVHR*CLLMGHHHHMQQC 705
QL *PHRLFFIHL*LQ +R T ICGVHR*CLLMGHHHHMQQC
Sbjct: 420 QLP*PHRLFFIHL*LQALRDTHICGVHR*CLLMGHHHHMQQC 545
Score = 219 bits (473), Expect(2) = 1e-067
Identities = 92/105 (87%), Positives = 95/105 (90%)
Frame = -1 / -2
Query: 705 TLLHMVVVSHKEASSVDPTYTGAPDGLELQMNEKKPVGLLELSQQHYSMPGTETNQDMLW 526
TLLHMVVVSHK ASSVDPTY G P+GLELQMNEKKPVG ELSQ+HYSMPGTETNQDMLW
Sbjct: 545 TLLHMVVVSHKXASSVDPTYMGVPEGLELQMNEKKPVGSRELSQEHYSMPGTETNQDMLW 366
Query: 525 GG*QVYSAHSVLLTIFEIWT**PHHPFPLFISVLYPYVPFALYSL 391
GG* VYSAHSVLLTIFEIWT**PH PFPLFIS+LYPYVPFA L
Sbjct: 365 GG**VYSAHSVLLTIFEIWT**PHLPFPLFISLLYPYVPFAFILL 231
Score = 212 bits (458), Expect(2) = 2e-067
Identities = 90/104 (86%), Positives = 94/104 (90%)
Frame = +2 / +1
Query: 392 REYKAKGT*G*RTEINNGKG*CGY*VQISKIVSNTE*AEYTC*PPHSIS*LVSVPGIL*C 571
+ KAKGT*G*R EINNGKG CGY*VQISKIVSNTE*AEYT *PPHSIS*LVSVPGIL*C
Sbjct: 232 KRIKAKGT*G*RREINNGKGRCGY*VQISKIVSNTE*AEYTY*PPHSIS*LVSVPGIL*C 411
Query: 572 CWDSSSNPTGFFSFICSSNPSGAPVYVGSTDDASLWDTTTICNN 703
WDSS +PTGFFSFICSS PSG P+YVGSTDDA LWDTTTIC+N
Sbjct: 412 SWDSSRDPTGFFSFICSSKPSGTPIYVGSTDDAXLWDTTTICSN 543
Score = 164 bits (352), Expect(2) = 8e-052
Identities = 66/84 (78%), Positives = 68/84 (80%)
Frame = +3 / +2
Query: 402 KQKEHXXXXXXXXXXXXDVATRSKSQKSSAIQNEPSTPANPPTAYPDWSQFQAYYNAAGT 581
+QKEH DVATRSKSQKSSAIQNE STP NPPTAYPDWSQFQAYYNA GT
Sbjct: 242 RQKEHKDRGEK*TMGKGDVATRSKSQKSSAIQNEQSTPTNPPTAYPDWSQFQAYYNAPGT 421
Query: 582 APVTPPAFFHSSVAPTHQGHPYMW 653
APVTPPAFFHSSVAP+ QGHPYMW
Sbjct: 422 APVTPPAFFHSSVAPSPQGHPYMW 493
Score = 134 bits (288), Expect(2) = 2e-039
Identities = 60/78 (76%), Positives = 61/78 (78%)
Frame = -3 / -1
Query: 637 P*WVGATDE*KKAGGVTGAVPAAL*YAWN*DQXXXXXXXXXXXXXSFCIADDF*DLDLVA 458
P* +GATDE*KKAGGVTGAVP AL*YAWN*DQ SFCIADDF*DLDLVA
Sbjct: 477 P*GLGATDE*KKAGGVTGAVPGAL*YAWN*DQSGYAVGGLVGVLCSFCIADDF*DLDLVA 298
Query: 457 TSPFPIVYFRPLPLCSFC 404
TSPFPIVYF PL LCSFC
Sbjct: 297 TSPFPIVYFSPLSLCSFC 244
Score = 87.2 bits (184), Expect(3) = 1e-081
Identities = 35/44 (79%), Positives = 37/44 (84%)
Frame = -1 / -1
Query: 315 KKHGNCF*LCNFFRNRACEGIEGQIQRLTSRRRDQSRWWWISTP 184
+K GN F CNFFR+RACEGIEGQIQ LTSRRRDQS W WISTP
Sbjct: 141 QKRGNSFLNCNFFRDRACEGIEGQIQCLTSRRRDQSTW*WISTP 10
Score = 77.6 bits (163), Expect(3) = 4e-077
Identities = 35/43 (81%), Positives = 35/43 (81%)
Frame = +1 / +1
Query: 184 WSGDPPPPGLVSSSAG*TLNLSFNAFTGSIPEEVAELEAIAVF 312
WSGDP P LVSSSAG* LNL FNAFTGSIPEEVA EAIA F
Sbjct: 10 WSGDPSPRRLVSSSAG*ALNLPFNAFTGSIPEEVAV*EAIATF 138
Score = 66.1 bits (138), Expect(2) = 2e-067
Identities = 25/30 (83%), Positives = 25/30 (83%)
Frame = +3 / +3
Query: 183 VEWRSTTTWIGLFFCWLDAESVLQCLHRLY 272
VEWRS TT IGLFFCWL ES LQCLHRLY
Sbjct: 9 VEWRSITT*IGLFFCWLGTESALQCLHRLY 98
Score = 62.9 bits (131), Expect(2) = 8e-052
Identities = 24/26 (92%), Positives = 25/26 (96%)
Frame = +2 / +2
Query: 185 GVEIHHHLDWSLLLLVRR*ICPSMPS 262
GVEIHHH+DWSLLLLVR *ICPSMPS
Sbjct: 11 GVEIHHHVDWSLLLLVRH*ICPSMPS 88
Score = 60.2 bits (125), Expect(2) = 1e-067
Identities = 26/31 (83%), Positives = 26/31 (83%)
Frame = -2 / -2
Query: 275 GIEPVKALKDRFSV*PAEEETNPGGGGSPLH 183
GIEPVKALK RFS *PAEEETN G GSPLH
Sbjct: 101 GIEPVKALKGRFSA*PAEEETNLRGDGSPLH 9
Score = 51.0 bits (105), Expect(2) = 2e-039
Identities = 21/26 (80%), Positives = 22/26 (84%)
Frame = -3 / -3
Query: 259 RH*RTDSASNQQKKRPIQVVVDLHST 182
RH*R DS NQQKKRPI VV+DLHST
Sbjct: 85 RH*RADSVPNQQKKRPIYVVMDLHST 8
Score = 24.0 bits (46), Expect(3) = 1e-081
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -3 / -2
Query: 382 KRTAFTCSVSNLD 344
K+ A +CSVSNLD
Sbjct: 203 KQNATSCSVSNLD 165
Score = 22.6 bits (43), Expect(3) = 4e-077
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +1 / +2
Query: 334 FGSYPDWIRNM 366
F +PDW RNM
Sbjct: 155 FLGHPDWTRNM 187
>gb|CD209204.1|CD209204 HS1_46_F10.g1_A012 Heat-shocked seedlings Sorghum bicolor cDNA
clone HS1_46_F10_A012 5', mRNA sequence
Length = 570
Score = 149 bits (321), Expect(4) = 4e-063
Identities = 61/71 (85%), Positives = 61/71 (85%)
Frame = +3 / +3
Query: 60 APGIPKSCGASAPTPLVDSSLPNTPEESFSATRLPPLCRAGVEWRSTTTWIGLFFCWLDA 239
AP IPK C ASAPT LVDSSLPNTPEESFS TRLPPLCRAGVEWRS TT IGLFFCWL
Sbjct: 117 APPIPKFCAASAPTLLVDSSLPNTPEESFSETRLPPLCRAGVEWRSITT*IGLFFCWLGT 296
Query: 240 ESVLQCLHRLY 272
ES LQCLHRLY
Sbjct: 297 ESALQCLHRLY 329
Score = 132 bits (283), Expect(4) = 5e-058
Identities = 54/64 (84%), Positives = 56/64 (87%)
Frame = -1 / -1
Query: 315 KKHGNCF*LCNFFRNRACEGIEGQIQRLTSRRRDQSRWWWISTPRQHGREEEDESRRRIP 136
+K GN F CNFFR+RACEGIEGQIQ LTSRRRDQS W WISTPRQ GREEEDESRRRIP
Sbjct: 372 QKRGNSFLNCNFFRDRACEGIEGQIQCLTSRRRDQSTW*WISTPRQPGREEEDESRRRIP 193
Query: 135 PACS 124
PACS
Sbjct: 192 PACS 181
Score = 131 bits (280), Expect(4) = 9e-055
Identities = 57/67 (85%), Positives = 60/67 (89%)
Frame = -3 / -3
Query: 259 RH*RTDSASNQQKKRPIQVVVDLHSTPARQRGGRRVAEKDSSGVFGREESTSGVGAEAPQ 80
RH*R DS NQQKKRPI VV+DLHSTPARQRGGRRV+EKDSSGVFGREESTS VGAEA Q
Sbjct: 316 RH*RADSVPNQQKKRPIYVVMDLHSTPARQRGGRRVSEKDSSGVFGREESTSRVGAEAAQ 137
Query: 79 DLGIPGA 59
+LGI GA
Sbjct: 136 NLGIGGA 116
Score = 128 bits (274), Expect(4) = 1e-052
Identities = 59/69 (85%), Positives = 59/69 (85%)
Frame = +1 / +1
Query: 106 SSTPLFRTRRRNPSPRLVFLLSAVLAWSGDPPPPGLVSSSAG*TLNLSFNAFTGSIPEEV 285
SSTPL RTRRRNPSPRLVFLLSA LAWSGDP P LVSSSAG* LNL FNAFTGSIPEEV
Sbjct: 163 SSTPLCRTRRRNPSPRLVFLLSAGLAWSGDPSPRRLVSSSAG*ALNLPFNAFTGSIPEEV 342
Query: 286 AELEAIAVF 312
A EAIA F
Sbjct: 343 AV*EAIATF 369
Score = 111 bits (237), Expect(4) = 1e-046
Identities = 49/58 (84%), Positives = 51/58 (87%)
Frame = -2 / -2
Query: 275 GIEPVKALKDRFSV*PAEEETNPGGGGSPLHASTAERRKTSRGEGFLRRVRKRGVDER 102
GIEPVKALK RFS *PAEEETN G GSPLHAS AERRKTS GEGFLRRVR+RGVDE+
Sbjct: 332 GIEPVKALKGRFSA*PAEEETNLRGDGSPLHASPAERRKTSLGEGFLRRVRQRGVDEQ 159
Score = 111 bits (237), Expect(3) = 2e-035
Identities = 48/65 (73%), Positives = 50/65 (76%)
Frame = +2 / +2
Query: 68 NSQIXXXXXXXXXXXXXSSEHAGGILLRDSSSSSLPCWRGVEIHHHLDWSLLLLVRR*IC 247
NSQI S+EHAGGILLRDSSSSSLP WRGVEIHHH+DWSLLLLVR *IC
Sbjct: 125 NSQILRRLRPNPARRLLSAEHAGGILLRDSSSSSLPGWRGVEIHHHVDWSLLLLVRH*IC 304
Query: 248 PSMPS 262
PSMPS
Sbjct: 305 PSMPS 319
Score = 89.0 bits (188), Expect(4) = 4e-063
Identities = 34/40 (85%), Positives = 37/40 (92%)
Frame = +1 / +3
Query: 400 QSKRNIRVEDGNKQWERVMWLLGPNLKNRQQYRMSRVHLL 519
+ KRNIR+E+ NKQWER MWLLGPNLKNRQQYRMSRVHLL
Sbjct: 450 KGKRNIRIEERNKQWEREMWLLGPNLKNRQQYRMSRVHLL 569
Score = 86.8 bits (183), Expect(4) = 5e-058
Identities = 36/41 (87%), Positives = 37/41 (90%)
Frame = -1 / -2
Query: 513 VYSAHSVLLTIFEIWT**PHHPFPLFISVLYPYVPFALYSL 391
VYSAHSVLLTIFEIWT**PH PFPLFIS+LYPYVPFA L
Sbjct: 563 VYSAHSVLLTIFEIWT**PHLPFPLFISLLYPYVPFAFILL 441
Score = 81.7 bits (172), Expect(4) = 9e-055
Identities = 34/38 (89%), Positives = 35/38 (92%)
Frame = -2 / -3
Query: 518 SRCTRLILYC*RFLRFGPSSHITLSHCLFPSSTLMFLL 405
SRCT LILYC*RFLRFGPSSHI+LSHCLF SS LMFLL
Sbjct: 568 SRCTLLILYC*RFLRFGPSSHISLSHCLFLSSILMFLL 455
Score = 79.4 bits (167), Expect(4) = 1e-052
Identities = 36/41 (87%), Positives = 37/41 (90%)
Frame = +2 / +1
Query: 392 REYKAKGT*G*RTEINNGKG*CGY*VQISKIVSNTE*AEYT 514
+ KAKGT*G*R EINNGKG CGY*VQISKIVSNTE*AEYT
Sbjct: 442 KRIKAKGT*G*RREINNGKGRCGY*VQISKIVSNTE*AEYT 564
Score = 76.7 bits (161), Expect(4) = 1e-046
Identities = 30/32 (93%), Positives = 30/32 (93%)
Frame = -3 / -1
Query: 499 FCIADDF*DLDLVATSPFPIVYFRPLPLCSFC 404
FCIADDF*DLDLVATSPFPIVYF PL LCSFC
Sbjct: 549 FCIADDF*DLDLVATSPFPIVYFSPLSLCSFC 454
Score = 51.0 bits (105), Expect(3) = 2e-035
Identities = 24/38 (63%), Positives = 25/38 (65%)
Frame = +3 / +2
Query: 402 KQKEHXXXXXXXXXXXXDVATRSKSQKSSAIQNEPSTP 515
+QKEH DVATRSKSQKSSAIQNE STP
Sbjct: 452 RQKEHKDRGEK*TMGKGDVATRSKSQKSSAIQNEQSTP 565
Score = 35.9 bits (72), Expect(4) = 5e-058
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -3 / -3
Query: 64 GAQAVER*CQWTRNGVSTPY 5
G VE *C+W NGVSTPY
Sbjct: 124 GGAGVES*CEWRGNGVSTPY 65
Score = 34.1 bits (68), Expect(4) = 4e-063
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +1 / +1
Query: 1 GSRECSHHFLSIDIS 45
GSREC HHFLSI IS
Sbjct: 61 GSRECLHHFLSIHIS 105
Score = 31.8 bits (63), Expect(4) = 9e-055
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = -1 / -1
Query: 45 ADVNGQEMV*ALPT 4
ADVNG+EMV*ALPT
Sbjct: 105 ADVNGEEMV*ALPT 64
Score = 30.9 bits (61), Expect(4) = 1e-052
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +3 / +3
Query: 3 K*GVLTPFLVH*HQRSTACAP 65
+*GVLTPF +H HQ ST P
Sbjct: 63 E*GVLTPFPLHSHQLSTPAPP 125
Score = 30.4 bits (60), Expect(4) = 1e-046
Identities = 10/14 (71%), Positives = 13/14 (92%)
Frame = -2 / -2
Query: 44 LMSMDKKWCEHSLL 3
LM M++KWC+HSLL
Sbjct: 104 LM*MERKWCKHSLL 63
Score = 27.6 bits (54), Expect(4) = 5e-058
Identities = 11/13 (84%), Positives = 11/13 (84%)
Frame = -3 / -2
Query: 382 KRTAFTCSVSNLD 344
K AFTCSVSNLD
Sbjct: 434 KDRAFTCSVSNLD 396
Score = 27.2 bits (53), Expect(4) = 4e-063
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +1 / +2
Query: 334 FGSYPDWIRNM*KL 375
F +PDW RNM*KL
Sbjct: 386 FLGHPDWTRNM*KL 427
Score = 27.2 bits (53), Expect(4) = 9e-055
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -2 / -1
Query: 374 SFHMFRIQSGYDPKI 330
SFHMFR+QSG K+
Sbjct: 426 SFHMFRVQSG*PKKL 382
Score = 26.3 bits (51), Expect(4) = 1e-052
Identities = 10/11 (90%), Positives = 11/11 (100%)
Frame = +3 / +1
Query: 345 SRLDTEHVKAV 377
SRLDTEHVKA+
Sbjct: 397 SRLDTEHVKAL 429
Score = 25.8 bits (50), Expect(3) = 2e-035
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +2 / +3
Query: 344 IQIGYGTCES 373
IQIG+GTCES
Sbjct: 396 IQIGHGTCES 425
Score = 25.8 bits (50), Expect(4) = 1e-046
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -1 / -3
Query: 375 QLSHVPYPIWI 343
+LSHVP PIW+
Sbjct: 427 ELSHVPCPIWM 395
>gb|BG464223.1|BG464223 EM1_71_B05.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 528
Score = 152 bits (326), Expect = 6e-036
Identities = 53/60 (88%), Positives = 54/60 (90%)
Frame = -2 / -3
Query: 704 HCCIWWWCPIRRHHLWTPHIRVPLMGWSYR*MKKSRWGYWSCPSSIIVCLELRPIRICCG 525
HCCIWWWCPIRRHHLWTPHI V L WSYR*MKKSRWG+ SCP SIIVCLELRPIRICCG
Sbjct: 187 HCCIWWWCPIRRHHLWTPHIWVSLRAWSYR*MKKSRWGHGSCPRSIIVCLELRPIRICCG 8
Score = 138 bits (296), Expect = 9e-032
Identities = 56/62 (90%), Positives = 57/62 (91%)
Frame = +1 / +3
Query: 520 TPPQHILIGLSSRHTIMLLGQLQ*PHRLFFIHL*LQPIRGTRICGVHR*CLLMGHHHHMQ 699
T PQHILIGLSSRHTIMLLGQL *PHRLFFIHL*LQ +R T ICGVHR*CLLMGHHHHMQ
Sbjct: 3 TRPQHILIGLSSRHTIMLLGQLP*PHRLFFIHL*LQALRDTHICGVHR*CLLMGHHHHMQ 182
Query: 700 QC 705
QC
Sbjct: 183 QC 188
Score = 134 bits (287), Expect = 1e-030
Identities = 54/60 (90%), Positives = 56/60 (93%)
Frame = -1 / -2
Query: 705 TLLHMVVVSHKEASSVDPTYTGAPDGLELQMNEKKPVGLLELSQQHYSMPGTETNQDMLW 526
TLLHMVVVSHKEASSVDPTY G P+GLELQMNEKKPVG ELSQ+HYSMPGTETNQDMLW
Sbjct: 188 TLLHMVVVSHKEASSVDPTYMGVPEGLELQMNEKKPVGSRELSQEHYSMPGTETNQDMLW 9
Score = 132 bits (282), Expect = 7e-030
Identities = 52/59 (88%), Positives = 55/59 (93%)
Frame = +2 / +1
Query: 527 HSIS*LVSVPGIL*CCWDSSSNPTGFFSFICSSNPSGAPVYVGSTDDASLWDTTTICNN 703
HSIS*LVSVPGIL*C WDSS +PTGFFSFICSS PSG P+YVGSTDDASLWDTTTIC+N
Sbjct: 10 HSIS*LVSVPGIL*CSWDSSRDPTGFFSFICSSKPSGTPIYVGSTDDASLWDTTTICSN 186
Score = 106 bits (227), Expect = 3e-022
Identities = 39/42 (92%), Positives = 40/42 (95%)
Frame = +3 / +2
Query: 528 TAYPDWSQFQAYYNAAGTAPVTPPAFFHSSVAPTHQGHPYMW 653
TAYPDWSQFQAYYNA GTAPVTPPAFFHSSVAP+ QGHPYMW
Sbjct: 11 TAYPDWSQFQAYYNAPGTAPVTPPAFFHSSVAPSPQGHPYMW 136
>gb|BG649135.1|BG649135 EM1_56_A05.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA
sequence
Length = 527
Score = 152 bits (326), Expect = 6e-036
Identities = 53/60 (88%), Positives = 54/60 (90%)
Frame = -2 / -2
Query: 704 HCCIWWWCPIRRHHLWTPHIRVPLMGWSYR*MKKSRWGYWSCPSSIIVCLELRPIRICCG 525
HCCIWWWCPIRRHHLWTPHI V L WSYR*MKKSRWG+ SCP SIIVCLELRPIRICCG
Sbjct: 187 HCCIWWWCPIRRHHLWTPHIWVSLRAWSYR*MKKSRWGHGSCPRSIIVCLELRPIRICCG 8
Score = 138 bits (296), Expect = 9e-032
Identities = 56/62 (90%), Positives = 57/62 (91%)
Frame = +1 / +3
Query: 520 TPPQHILIGLSSRHTIMLLGQLQ*PHRLFFIHL*LQPIRGTRICGVHR*CLLMGHHHHMQ 699
T PQHILIGLSSRHTIMLLGQL *PHRLFFIHL*LQ +R T ICGVHR*CLLMGHHHHMQ
Sbjct: 3 TRPQHILIGLSSRHTIMLLGQLP*PHRLFFIHL*LQALRDTHICGVHR*CLLMGHHHHMQ 182
Query: 700 QC 705
QC
Sbjct: 183 QC 188
Score = 134 bits (287), Expect = 1e-030
Identities = 54/60 (90%), Positives = 56/60 (93%)
Frame = -1 / -1
Query: 705 TLLHMVVVSHKEASSVDPTYTGAPDGLELQMNEKKPVGLLELSQQHYSMPGTETNQDMLW 526
TLLHMVVVSHKEASSVDPTY G P+GLELQMNEKKPVG ELSQ+HYSMPGTETNQDMLW
Sbjct: 188 TLLHMVVVSHKEASSVDPTYMGVPEGLELQMNEKKPVGSRELSQEHYSMPGTETNQDMLW 9
Score = 132 bits (282), Expect = 7e-030
Identities = 52/59 (88%), Positives = 55/59 (93%)
Frame = +2 / +1
Query: 527 HSIS*LVSVPGIL*CCWDSSSNPTGFFSFICSSNPSGAPVYVGSTDDASLWDTTTICNN 703
HSIS*LVSVPGIL*C WDSS +PTGFFSFICSS PSG P+YVGSTDDASLWDTTTIC+N
Sbjct: 10 HSIS*LVSVPGIL*CSWDSSRDPTGFFSFICSSKPSGTPIYVGSTDDASLWDTTTICSN 186
Score = 106 bits (227), Expect = 3e-022
Identities = 39/42 (92%), Positives = 40/42 (95%)
Frame = +3 / +2
Query: 528 TAYPDWSQFQAYYNAAGTAPVTPPAFFHSSVAPTHQGHPYMW 653
TAYPDWSQFQAYYNA GTAPVTPPAFFHSSVAP+ QGHPYMW
Sbjct: 11 TAYPDWSQFQAYYNAPGTAPVTPPAFFHSSVAPSPQGHPYMW 136
>gb|CW428236.1|CW428236 fsbb001f140l08k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f140l08, DNA
sequence
Length = 560
Score = 123 bits (263), Expect(2) = 2e-031
Identities = 50/57 (87%), Positives = 50/57 (87%)
Frame = +3 / +3
Query: 60 APGIPKSCGASAPTPLVDSSLPNTPEESFSATRLPPLCRAGVEWRSTTTWIGLFFCW 230
AP IPK C ASAPT LVDSSLPNTPEESFS TRLPPLCRAGVEWRS TT IGLFFCW
Sbjct: 351 APPIPKFCAASAPTLLVDSSLPNTPEESFSETRLPPLCRAGVEWRSITT*IGLFFCW 521
Score = 115 bits (246), Expect(2) = 9e-030
Identities = 50/57 (87%), Positives = 53/57 (92%)
Frame = -3 / -2
Query: 229 QQKKRPIQVVVDLHSTPARQRGGRRVAEKDSSGVFGREESTSGVGAEAPQDLGIPGA 59
QQKKRPI VV+DLHSTPARQRGGRRV+EKDSSGVFGREESTS VGAEA Q+LGI GA
Sbjct: 520 QQKKRPIYVVMDLHSTPARQRGGRRVSEKDSSGVFGREESTSRVGAEAAQNLGIGGA 350
Score = 90.4 bits (191), Expect(2) = 9e-021
Identities = 39/55 (70%), Positives = 41/55 (74%)
Frame = +2 / +2
Query: 68 NSQIXXXXXXXXXXXXXSSEHAGGILLRDSSSSSLPCWRGVEIHHHLDWSLLLLV 232
NSQI S+EHAGGILLRDSSSSSLP WRGVEIHHH+DWSLLLLV
Sbjct: 359 NSQILRRLRPNPARRLLSAEHAGGILLRDSSSSSLPGWRGVEIHHHVDWSLLLLV 523
Score = 35.9 bits (72), Expect(2) = 9e-030
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -3 / -2
Query: 64 GAQAVER*CQWTRNGVSTPY 5
G VE *C+W NGVSTPY
Sbjct: 358 GGAGVES*CEWRGNGVSTPY 299
Score = 34.1 bits (68), Expect(2) = 2e-031
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +1 / +1
Query: 1 GSRECSHHFLSIDIS 45
GSREC HHFLSI IS
Sbjct: 295 GSRECLHHFLSIHIS 339
Score = 30.9 bits (61), Expect(2) = 9e-021
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +3 / +3
Query: 3 K*GVLTPFLVH*HQRSTACAP 65
+*GVLTPF +H HQ ST P
Sbjct: 297 E*GVLTPFPLHSHQLSTPAPP 359
>gb|CW415759.1|CW415759 fsbb001f116a03f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f116a03, DNA
sequence
Length = 344
Score = 118 bits (252), Expect(2) = 5e-030
Identities = 49/56 (87%), Positives = 49/56 (87%)
Frame = +3 / +2
Query: 60 APGIPKSCGASAPTPLVDSSLPNTPEESFSATRLPPLCRAGVEWRSTTTWIGLFFC 227
AP IPK C ASAPT LVDSSLPNTPEESFS TRLPPLCRAGVEWRS TT IGLFFC
Sbjct: 176 APPIPKFCAASAPTLLVDSSLPNTPEESFSETRLPPLCRAGVEWRSITT*IGLFFC 343
Score = 110 bits (235), Expect(2) = 3e-028
Identities = 48/56 (85%), Positives = 51/56 (91%)
Frame = -3 / -3
Query: 226 QKKRPIQVVVDLHSTPARQRGGRRVAEKDSSGVFGREESTSGVGAEAPQDLGIPGA 59
QKKRPI VV+DLHSTPARQRGG RV+EKDSSGVFGREESTS VGAEA Q+LGI GA
Sbjct: 342 QKKRPIYVVMDLHSTPARQRGGSRVSEKDSSGVFGREESTSRVGAEAAQNLGIGGA 175
Score = 35.9 bits (72), Expect(2) = 3e-028
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -3 / -3
Query: 64 GAQAVER*CQWTRNGVSTPY 5
G VE *C+W NGVSTPY
Sbjct: 183 GGAGVES*CEWRGNGVSTPY 124
Score = 34.1 bits (68), Expect(2) = 5e-030
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +1 / +3
Query: 1 GSRECSHHFLSIDIS 45
GSREC HHFLSI IS
Sbjct: 120 GSRECLHHFLSIHIS 164
>gb|CW108361.1|CW108361 104_480_11097488_116_34496_032 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11097488, DNA
sequence
Length = 413
Score = 79.9 bits (168), Expect(2) = 1e-024
Identities = 31/42 (73%), Positives = 35/42 (83%)
Frame = +2 / +1
Query: 557 GIL*CCWDSSSNPTGFFSFICSSNPSGAPVYVGSTDDASLWD 682
GIL*C WDSS +PTGFFSFICSS PSG P+YVGST SL++
Sbjct: 160 GIL*CSWDSSRDPTGFFSFICSSKPSGTPIYVGSTGTFSLFN 285
Score = 79.9 bits (168), Expect(2) = 1e-023
Identities = 30/41 (73%), Positives = 34/41 (82%)
Frame = -2 / -2
Query: 671 RHHLWTPHIRVPLMGWSYR*MKKSRWGYWSCPSSIIVCLEL 549
R +LWTPHI V L WSYR*MKKSRWG+ SCP SIIVCL++
Sbjct: 274 RMYLWTPHIWVSLRAWSYR*MKKSRWGHGSCPRSIIVCLQI 152
Score = 79.4 bits (167), Expect(2) = 5e-024
Identities = 29/32 (90%), Positives = 30/32 (93%)
Frame = +3 / +2
Query: 558 AYYNAAGTAPVTPPAFFHSSVAPTHQGHPYMW 653
AYYNA GTAPVTPPAFFHSSVAP+ QGHPYMW
Sbjct: 161 AYYNAPGTAPVTPPAFFHSSVAPSPQGHPYMW 256
Score = 76.2 bits (160), Expect(2) = 1e-022
Identities = 30/41 (73%), Positives = 34/41 (82%)
Frame = -1 / -1
Query: 678 HKEASSVDPTYTGAPDGLELQMNEKKPVGLLELSQQHYSMP 556
++E VDPTY G P+GLELQMNEKKPVG ELSQ+HYSMP
Sbjct: 281 NRENVPVDPTYMGVPEGLELQMNEKKPVGSRELSQEHYSMP 159
Score = 75.3 bits (158), Expect(2) = 9e-021
Identities = 31/36 (86%), Positives = 32/36 (88%)
Frame = +1 / +3
Query: 556 RHTIMLLGQLQ*PHRLFFIHL*LQPIRGTRICGVHR 663
RHTIMLLGQL *PHRLFFIHL*LQ +R T ICGVHR
Sbjct: 159 RHTIMLLGQLP*PHRLFFIHL*LQALRDTHICGVHR 266
Score = 54.2 bits (112), Expect(2) = 1e-024
Identities = 20/25 (80%), Positives = 20/25 (80%)
Frame = +3 / +1
Query: 483 SSAIQNEPSTPANPPTAYPDWSQFQ 557
S QNE STP NPPTAYPDWSQFQ
Sbjct: 1 SFLFQNEQSTPTNPPTAYPDWSQFQ 75
Score = 52.8 bits (109), Expect(2) = 5e-024
Identities = 21/22 (95%), Positives = 22/22 (100%)
Frame = +1 / +2
Query: 493 YRMSRVHLLTPPQHILIGLSSR 558
+RMSRVHLLTPPQHILIGLSSR
Sbjct: 11 FRMSRVHLLTPPQHILIGLSSR 76
Score = 51.0 bits (105), Expect(2) = 1e-023
Identities = 20/21 (95%), Positives = 20/21 (95%)
Frame = -1 / -2
Query: 558 PGTETNQDMLWGG*QVYSAHS 496
PGTETNQDMLWGG* VYSAHS
Sbjct: 76 PGTETNQDMLWGG**VYSAHS 14
Score = 51.0 bits (105), Expect(2) = 1e-022
Identities = 20/21 (95%), Positives = 20/21 (95%)
Frame = -2 / -3
Query: 557 LELRPIRICCGGVSRCTRLIL 495
LELRPIRICCGGVSRCT LIL
Sbjct: 75 LELRPIRICCGGVSRCTLLIL 13
Score = 46.0 bits (94), Expect(2) = 9e-021
Identities = 20/23 (86%), Positives = 22/23 (95%)
Frame = +2 / +3
Query: 494 TE*AEYTC*PPHSIS*LVSVPGI 562
+E*AEYT *PPHSIS*LVSVPG+
Sbjct: 12 SE*AEYTY*PPHSIS*LVSVPGV 80
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 929,348,025
Number of Sequences: 832831
Number of extensions: 15163238
Number of successful extensions: 178247
Number of sequences better than 1.0e-020: 9
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 21386462760
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)