TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBJ7h03.xg.2.1
(487 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN127879.1|CN127879 RHOH1_25_D05.g1_A002 Acid- and alkal... 95 4e-032
gb|BE360223.1|BE360223 DG1_62_C07.b1_A002 Dark Grown 1 (DG1... 95 4e-032
gb|CN140719.1|CN140719 OX1_46_B10.g1_A002 Oxidatively-stres... 92 2e-031
gb|CN129542.1|CN129542 RHOH1_36_B03.b3_A002 Acid- and alkal... 88 5e-030
gb|CN129628.1|CN129628 RHOH1_36_B03.g1_A002 Acid- and alkal... 88 5e-030
gb|AW671472.1|AW671472 LG1_347_C12.b1_A002 Light Grown 1 (L... 98 1e-029
gb|CW071272.1|CW071272 104_323_10591451_114_30471 Sorghum m... 73 5e-027
gb|CN127798.1|CN127798 RHOH1_25_D05.b1_A002 Acid- and alkal... 76 2e-026
gb|AW287022.2|AW287022 LG1_264_E07.b1_A002 Light Grown 1 (L... 95 1e-025
gb|CW376890.1|CW376890 fsbb001f054p19f0 Sorghum methylation... 69 1e-024
gb|CW497105.1|CW497105 fsbb001f289k18f0 Sorghum methylation... 73 5e-022
>gb|CN127879.1|CN127879 RHOH1_25_D05.g1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_25_D05_A002 5', mRNA sequence
Length = 692
Score = 95.0 bits (201), Expect(2) = 4e-032
Identities = 36/74 (48%), Positives = 45/74 (60%)
Frame = +2 / +3
Query: 74 FTGTQXXCAECDKXXXXIDLLXXXXXTYHKTCFXXXHCKGILSXXSYSSMXXXLYCKTHF 253
F+GTQ C C K +D L +H++CF HCK LS +YSS YCK HF
Sbjct: 69 FSGTQQKCKVCTKTVYPMDQLSTDGVAFHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 248
Query: 254 EQLFKETGSFSKNF 295
EQLFKETGS++K+F
Sbjct: 249 EQLFKETGSYNKSF 290
Score = 63.8 bits (133), Expect(2) = 4e-032
Identities = 26/50 (52%), Positives = 31/50 (62%)
Frame = +2 / +3
Query: 329 LTRAPSKLSSXFSGTXXXXXACQKTVYPLEXLTLEXXSYHKXXXXXSHGG 478
LTR+PSK + FSGT C KT YPLE +T+E +YHK SHGG
Sbjct: 339 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGG 488
>gb|BE360223.1|BE360223 DG1_62_C07.b1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 597
Score = 95.0 bits (201), Expect(2) = 4e-032
Identities = 36/74 (48%), Positives = 45/74 (60%)
Frame = +2 / +2
Query: 74 FTGTQXXCAECDKXXXXIDLLXXXXXTYHKTCFXXXHCKGILSXXSYSSMXXXLYCKTHF 253
F+GTQ C C K +D L +H++CF HCK LS +YSS YCK HF
Sbjct: 149 FSGTQQKCKVCTKTVYPMDQLSTDGVAFHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 328
Query: 254 EQLFKETGSFSKNF 295
EQLFKETGS++K+F
Sbjct: 329 EQLFKETGSYNKSF 370
Score = 63.8 bits (133), Expect(2) = 4e-032
Identities = 26/50 (52%), Positives = 31/50 (62%)
Frame = +2 / +2
Query: 329 LTRAPSKLSSXFSGTXXXXXACQKTVYPLEXLTLEXXSYHKXXXXXSHGG 478
LTR+PSK + FSGT C KT YPLE +T+E +YHK SHGG
Sbjct: 419 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGG 568
>gb|CN140719.1|CN140719 OX1_46_B10.g1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_46_B10_A002 5', mRNA sequence
Length = 544
Score = 92.3 bits (195), Expect(2) = 2e-031
Identities = 38/86 (44%), Positives = 48/86 (55%)
Frame = +2 / +1
Query: 38 SHRQAGVGTMSSFTGTQXXCAECDKXXXXIDLLXXXXXTYHKTCFXXXHCKGILSXXSYS 217
S +AG F+GTQ C C K +D L +H++CF HCK LS +YS
Sbjct: 22 SEARAGELGEKMFSGTQQKCKVCTKTVYPMDQLSTDGVAFHRSCFKCQHCKSTLSLSNYS 201
Query: 218 SMXXXLYCKTHFEQLFKETGSFSKNF 295
S YCK HFEQLFKET S++K+F
Sbjct: 202 SFEGVPYCKAHFEQLFKETVSYNKSF 279
Score = 63.8 bits (133), Expect(2) = 2e-031
Identities = 26/50 (52%), Positives = 31/50 (62%)
Frame = +2 / +1
Query: 329 LTRAPSKLSSXFSGTXXXXXACQKTVYPLEXLTLEXXSYHKXXXXXSHGG 478
LTR+PSK + FSGT C KT YPLE +T+E +YHK SHGG
Sbjct: 328 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGG 477
>gb|CN129542.1|CN129542 RHOH1_36_B03.b3_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_36_B03_A002 3', mRNA sequence
Length = 819
Score = 87.7 bits (185), Expect(2) = 5e-030
Identities = 36/74 (48%), Positives = 44/74 (59%)
Frame = +2 / +2
Query: 74 FTGTQXXCAECDKXXXXIDLLXXXXXTYHKTCFXXXHCKGILSXXSYSSMXXXLYCKTHF 253
F+GTQ C K +D L H++CF HCK LS +YSS YCK HF
Sbjct: 11 FSGTQQKCKVRTKTVYPMDQLSTXGVALHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 190
Query: 254 EQLFKETGSFSKNF 295
EQLFKETGS++K+F
Sbjct: 191 EQLFKETGSYNKSF 232
Score = 63.8 bits (133), Expect(2) = 5e-030
Identities = 26/50 (52%), Positives = 31/50 (62%)
Frame = +2 / +2
Query: 329 LTRAPSKLSSXFSGTXXXXXACQKTVYPLEXLTLEXXSYHKXXXXXSHGG 478
LTR+PSK + FSGT C KT YPLE +T+E +YHK SHGG
Sbjct: 281 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGG 430
>gb|CN129628.1|CN129628 RHOH1_36_B03.g1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_36_B03_A002 5', mRNA sequence
Length = 784
Score = 87.7 bits (185), Expect(2) = 5e-030
Identities = 35/74 (47%), Positives = 43/74 (58%)
Frame = +2 / +2
Query: 74 FTGTQXXCAECDKXXXXIDLLXXXXXTYHKTCFXXXHCKGILSXXSYSSMXXXLYCKTHF 253
F+GTQ C K +D L H++CF HCK LS +YSS YCK HF
Sbjct: 113 FSGTQQKCKVRTKTVYPMDQLSTDGVALHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 292
Query: 254 EQLFKETGSFSKNF 295
EQLFKETGS++K+F
Sbjct: 293 EQLFKETGSYNKSF 334
Score = 63.8 bits (133), Expect(2) = 5e-030
Identities = 26/50 (52%), Positives = 31/50 (62%)
Frame = +2 / +2
Query: 329 LTRAPSKLSSXFSGTXXXXXACQKTVYPLEXLTLEXXSYHKXXXXXSHGG 478
LTR+PSK + FSGT C KT YPLE +T+E +YHK SHGG
Sbjct: 383 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGG 532
>gb|AW671472.1|AW671472 LG1_347_C12.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 674
Score = 98.2 bits (208), Expect(2) = 1e-029
Identities = 37/81 (45%), Positives = 44/81 (54%)
Frame = +2 / +1
Query: 53 GVGTMSSFTGTQXXCAECDKXXXXIDLLXXXXXTYHKTCFXXXHCKGILSXXSYSSMXXX 232
G +SF GT C CDK +D L YHK CF HCKG L +Y+S
Sbjct: 211 GPAMATSFQGTTTKCTACDKTVYLVDKLTADNRIYHKACFRCHHCKGTLKLANYNSFEGV 390
Query: 233 LYCKTHFEQLFKETGSFSKNF 295
LYC+ HF+QLFK TGS K+F
Sbjct: 391 LYCRPHFDQLFKRTGSLDKSF 453
Score = 51.9 bits (107), Expect(2) = 1e-029
Identities = 20/44 (45%), Positives = 25/44 (56%)
Frame = +2 / +1
Query: 347 KLSSXFSGTXXXXXACQKTVYPLEXLTLEXXSYHKXXXXXSHGG 478
K+SS F+GT C KTVYP+E +T+ YHK HGG
Sbjct: 511 KVSSAFAGTREKCVGCSKTVYPIEKVTVNNTMYHKSCFKCCHGG 642
>gb|CW071272.1|CW071272 104_323_10591451_114_30471 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10591451, DNA
sequence
Length = 734
Score = 73.5 bits (154), Expect(2) = 5e-027
Identities = 30/39 (76%), Positives = 30/39 (76%)
Frame = +2 / -1
Query: 185 CKGILSXXSYSSMXXXLYCKTHFEQLFKETGSFSKNFTP 301
C G L SYSSM LYCKTHFEQLFKETGSFSK FTP
Sbjct: 239 CVGRLQMCSYSSMDGVLYCKTHFEQLFKETGSFSKKFTP 123
Score = 69.3 bits (145), Expect(2) = 5e-027
Identities = 27/44 (61%), Positives = 27/44 (61%)
Frame = +2 / -1
Query: 71 SFTGTQXXCAECDKXXXXIDLLXXXXXTYHKTCFXXXHCKGILS 202
SFTGTQ C CDK IDLL YHKTCF HCKGILS
Sbjct: 512 SFTGTQDKCTACDKTVHFIDLLTADGVIYHKTCFKCSHCKGILS 381
Score = 65.2 bits (136), Expect(3) = 7e-025
Identities = 27/44 (61%), Positives = 30/44 (68%)
Frame = -2 / +1
Query: 201 ERIPLQWXXXKHVLW*VXXSXXRRSMXXXXLSHSAHXSXVPVKE 70
ERIPLQW KHVLW*+ S RRSM LSH+ H S VPVK+
Sbjct: 382 ERIPLQWLHLKHVLW*MTPSAVRRSMKWTVLSHAVHLSCVPVKD 513
Score = 65.2 bits (136), Expect(3) = 7e-025
Identities = 28/33 (84%), Positives = 28/33 (84%)
Frame = -2 / +1
Query: 300 GVKFLEKLPVSLKSCSKWVLQYXXXSMEE*LXI 202
GV FLEKLPVSLKSCSKWVLQY SMEE*L I
Sbjct: 124 GVNFLEKLPVSLKSCSKWVLQYSTPSMEE*LHI 222
Score = 23.1 bits (44), Expect(3) = 7e-025
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -3 / +2
Query: 71 KTWCRRL 51
KTWCRRL
Sbjct: 509 KTWCRRL 529
>gb|CN127798.1|CN127798 RHOH1_25_D05.b1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_25_D05_A002 3', mRNA sequence
Length = 720
Score = 75.8 bits (159), Expect(2) = 2e-026
Identities = 27/47 (57%), Positives = 34/47 (72%)
Frame = +2 / +2
Query: 155 YHKTCFXXXHCKGILSXXSYSSMXXXLYCKTHFEQLFKETGSFSKNF 295
+H++CF HCK LS +YSS YCK HFEQLFKETGS++K+F
Sbjct: 8 FHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHFEQLFKETGSYNKSF 148
Score = 63.8 bits (133), Expect(2) = 2e-026
Identities = 26/50 (52%), Positives = 31/50 (62%)
Frame = +2 / +2
Query: 329 LTRAPSKLSSXFSGTXXXXXACQKTVYPLEXLTLEXXSYHKXXXXXSHGG 478
LTR+PSK + FSGT C KT YPLE +T+E +YHK SHGG
Sbjct: 197 LTRSPSKAARMFSGTQDKCATCGKTAYPLEKVTVEEKAYHKSCFKCSHGG 346
>gb|AW287022.2|AW287022 LG1_264_E07.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 511
Score = 95.0 bits (201), Expect(2) = 1e-025
Identities = 36/74 (48%), Positives = 45/74 (60%)
Frame = +2 / +2
Query: 74 FTGTQXXCAECDKXXXXIDLLXXXXXTYHKTCFXXXHCKGILSXXSYSSMXXXLYCKTHF 253
F+GTQ C C K +D L +H++CF HCK LS +YSS YCK HF
Sbjct: 149 FSGTQQKCKVCTKTVYPMDQLSTDGVAFHRSCFKCQHCKSTLSLSNYSSFEGVPYCKAHF 328
Query: 254 EQLFKETGSFSKNF 295
EQLFKETGS++K+F
Sbjct: 329 EQLFKETGSYNKSF 370
Score = 41.8 bits (85), Expect(2) = 1e-025
Identities = 17/30 (56%), Positives = 19/30 (63%)
Frame = +2 / +2
Query: 329 LTRAPSKLSSXFSGTXXXXXACQKTVYPLE 418
LTR+PSK + FSGT C KT YPLE
Sbjct: 419 LTRSPSKAARMFSGTQDKCATCGKTAYPLE 508
>gb|CW376890.1|CW376890 fsbb001f054p19f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f054p19, DNA
sequence
Length = 642
Score = 69.3 bits (145), Expect(2) = 1e-024
Identities = 27/44 (61%), Positives = 27/44 (61%)
Frame = +2 / +3
Query: 71 SFTGTQXXCAECDKXXXXIDLLXXXXXTYHKTCFXXXHCKGILS 202
SFTGTQ C CDK IDLL YHKTCF HCKGILS
Sbjct: 261 SFTGTQDKCTACDKTVHFIDLLTADGVIYHKTCFKCSHCKGILS 392
Score = 65.2 bits (136), Expect(2) = 1e-024
Identities = 27/35 (77%), Positives = 27/35 (77%)
Frame = +2 / +3
Query: 185 CKGILSXXSYSSMXXXLYCKTHFEQLFKETGSFSK 289
C G L SYSSM LYCKTHFEQLFKETGSFSK
Sbjct: 534 CVGRLQMCSYSSMDGVLYCKTHFEQLFKETGSFSK 638
Score = 65.2 bits (136), Expect(3) = 1e-023
Identities = 27/44 (61%), Positives = 30/44 (68%)
Frame = -2 / -3
Query: 201 ERIPLQWXXXKHVLW*VXXSXXRRSMXXXXLSHSAHXSXVPVKE 70
ERIPLQW KHVLW*+ S RRSM LSH+ H S VPVK+
Sbjct: 391 ERIPLQWLHLKHVLW*MTPSAVRRSMKWTVLSHAVHLSCVPVKD 260
Score = 60.6 bits (126), Expect(3) = 1e-023
Identities = 26/30 (86%), Positives = 26/30 (86%)
Frame = -2 / -3
Query: 291 FLEKLPVSLKSCSKWVLQYXXXSMEE*LXI 202
FLEKLPVSLKSCSKWVLQY SMEE*L I
Sbjct: 640 FLEKLPVSLKSCSKWVLQYSTPSMEE*LHI 551
Score = 23.1 bits (44), Expect(3) = 1e-023
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -3 / -1
Query: 71 KTWCRRL 51
KTWCRRL
Sbjct: 264 KTWCRRL 244
>gb|CW497105.1|CW497105 fsbb001f289k18f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f289k18, DNA
sequence
Length = 711
Score = 73.5 bits (154), Expect(2) = 5e-022
Identities = 30/39 (76%), Positives = 30/39 (76%)
Frame = +2 / +3
Query: 185 CKGILSXXSYSSMXXXLYCKTHFEQLFKETGSFSKNFTP 301
C G L SYSSM LYCKTHFEQLFKETGSFSK FTP
Sbjct: 156 CVGRLQMCSYSSMDGVLYCKTHFEQLFKETGSFSKKFTP 272
Score = 52.4 bits (108), Expect(2) = 5e-022
Identities = 22/28 (78%), Positives = 22/28 (78%)
Frame = +2 / +2
Query: 335 RAPSKLSSXFSGTXXXXXACQKTVYPLE 418
RAPSKLSS FSGT ACQKTVYPLE
Sbjct: 521 RAPSKLSSAFSGTQDKCAACQKTVYPLE 604
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 389,528,171
Number of Sequences: 832831
Number of extensions: 3229416
Number of successful extensions: 46533
Number of sequences better than 1.0e-020: 12
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 12921823404
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)