TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAT2e04.yg.2.1
(424 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF466200.2| Sorghum bicolor putative protein kinase gene... 139 2e-056
gb|BG159208.1|BG159208 RHIZ2_27_F09.b1_A003 Rhizome2 (RHIZ2... 135 4e-055
gb|CF489000.1|CF489000 POL1_53_C07.g1_A002 Pollen Sorghum b... 91 6e-047
gb|CW135635.1|CW135635 104_520_11117953_116_34836_009 Sorgh... 92 8e-045
gb|CW241144.1|CW241144 104_700_11218605_148_37542_093 Sorgh... 86 1e-041
gb|CF484299.1|CF484299 POL1_4_A06.g1_A002 Pollen Sorghum bi... 138 1e-038
gb|CW437404.1|CW437404 fsbb001f154i03k0 Sorghum methylation... 79 4e-036
gb|BZ334822.1|BZ334822 hx93g02.b1 WGS-SbicolorF (JM107 adap... 87 2e-035
gb|CW042123.1|CW042123 104_276_10506948_114_30476 Sorghum m... 79 1e-032
gb|CL700967.2|CL700967 SP__Ba0067G06.r SP__Ba Sorghum propi... 79 2e-032
gb|CW098901.1|CW098901 104_465_11003259_116_34374_006 Sorgh... 92 5e-029
gb|CW111980.1|CW111980 104_485_11104632_116_34538_086 Sorgh... 79 2e-026
gb|CW241143.1|CW241143 104_700_11218605_116_37545_093 Sorgh... 81 6e-026
gb|CN127712.1|CN127712 RHOH1_24_D05.g3_A002 Acid- and alkal... 114 6e-025
gb|CW209770.1|CW209770 104_640_11189431_116_37430_032 Sorgh... 82 4e-023
>gb|AF466200.2| Sorghum bicolor putative protein kinase gene, partial cds; putative Cf-2,
fertilization-independent endosperm proteins, hypothetical
protein, putative non-LTR retroelement reverse
transcriptase, OCL5 protein, tryptophan synthase
beta-subunit, hypothetical proteins, putative AP
endonuclease, putative RNA polymerase II complex component
SRB7, putative beta-1,3-glucanase, hypothetical protein,
TNP2-like protein, hypothetical protein, putative
phosphate/phosphoenolpyruvate translocator, putative
protein, hypothetical proteins, putative
galactosyltransferase family, hypothetical protein,
putative cytochrome P450 family, putative lipid transfer
protein, putative photoreceptor-interacting protein, and
hypothetical protein genes, complete cds; and hypothetical
protein gene, partial cds
Length = 202197
Score = 139 bits (299), Expect(3) = 2e-056
Identities = 56/75 (74%), Positives = 67/75 (89%)
Frame = +3 / +3
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQFP 182
ATALP +HPSFSL+QVLEMSE+W++R +P+ P+ LFIGILSA+NHFAERMA+RKTWMQ P
Sbjct: 169260 ATALPMSHPSFSLRQVLEMSEKWRSRPLPKGPVSLFIGILSASNHFAERMAVRKTWMQTP 169439
Query: 183 AIQSGNAVARFFVAL 227
I+S AVARFFVAL
Sbjct: 169440 EIKSSEAVARFFVAL 169484
Score = 97.7 bits (207), Expect(3) = 1e-028
Identities = 46/73 (63%), Positives = 51/73 (69%)
Frame = -1 / -3
Query: 220 TKNRATAFPDWIAGNCIQVFLIAMRSAKWFVAERMPMKRWIGSSGTVRAFHLSDISKTCW 41
TKNRATA D I+G CI VFL AMRSAK F A+ +PMKR G G+ R H SDIS+TC
Sbjct: 169477 TKNRATASEDLISGVCIHVFLTAMRSAK*FDADNIPMKRETGPFGSGRDLHFSDISRTCR 169298
Query: 40 REKLGCAFGSAVA 2
REKLG G AVA
Sbjct: 169297 REKLGWDIGRAVA 169259
Score = 93.6 bits (198), Expect(3) = 2e-056
Identities = 37/44 (84%), Positives = 41/44 (93%)
Frame = +3 / +3
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVHN 365
RKE+N LKKEAEYFGDIVILPFIDRYELVVLKT+AIC+YGV +
Sbjct: 170157 RKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVRH 170288
Score = 51.9 bits (107), Expect(3) = 1e-028
Identities = 22/29 (75%), Positives = 24/29 (82%)
Frame = -1 / -3
Query: 358 TPY*HIATVFRTTSSYRSMNGRMTMSPKY 272
TPY IA V RTTSS RS+NG+MTMSPKY
Sbjct: 170281 TPYSQIAIVLRTTSS*RSINGKMTMSPKY 170195
Score = 41.8 bits (85), Expect(3) = 2e-056
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = +3 / +2
Query: 357 VHNVTADYIMKCDDDTFVRLDI 422
V N+TA IMKCDDDTFVR+D+
Sbjct: 170597 VQNLTAANIMKCDDDTFVRVDM 170662
Score = 32.2 bits (64), Expect(3) = 1e-028
Identities = 15/23 (65%), Positives = 17/23 (73%)
Frame = -1 / -1
Query: 424 TISSRTKVSSSHFMM*SAVTLCT 356
TIS+ T VSSSHFMM +AV T
Sbjct: 170664 TISTLTNVSSSHFMMFAAVKFWT 170596
>gb|BG159208.1|BG159208 RHIZ2_27_F09.b1_A003 Rhizome2 (RHIZ2) Sorghum propinquum cDNA, mRNA
sequence
Length = 548
Score = 135 bits (290), Expect(2) = 4e-055
Identities = 56/77 (72%), Positives = 66/77 (85%)
Frame = +3 / +3
Query: 189 QSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVHNV 368
+S N VARFFVAL+ +KE+NA LKKEAE+F DIVI+PFID Y+LVVLKTVAI +YGV V
Sbjct: 303 KSSNIVARFFVALNGKKEVNAELKKEAEFFQDIVIVPFIDSYDLVVLKTVAIAEYGVRVV 482
Query: 369 TADYIMKCDDDTFVRLD 419
A Y+MKCDDDTFVR+D
Sbjct: 483 PAKYVMKCDDDTFVRID 533
Score = 99.1 bits (210), Expect(2) = 4e-055
Identities = 36/59 (61%), Positives = 50/59 (84%)
Frame = +3 / +3
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWMQF 179
A++LP +HP+F+ ++ LEMSE+WKA +P EP+ LFIGILSA +HFAERMA+RK+WM +
Sbjct: 120 ASSLPSSHPTFAPERYLEMSEQWKAPPLPTEPVELFIGILSAASHFAERMAVRKSWMMY 296
Score = 67.0 bits (140), Expect(2) = 1e-024
Identities = 31/57 (54%), Positives = 36/57 (63%)
Frame = -1 / -2
Query: 172 IQVFLIAMRSAKWFVAERMPMKRWIGSSGTVRAFHLSDISKTCWREKLGCAFGSAVA 2
I FL A+RSAKW AERMPMK GS G+ AFH SDIS+ K+GC G +A
Sbjct: 289 IHDFLTAIRSAKWLAAERMPMKSSTGSVGSGGAFHCSDISRYRSGAKVGCELGRELA 119
Score = 65.7 bits (137), Expect(2) = 1e-024
Identities = 28/74 (37%), Positives = 44/74 (59%)
Frame = -3 / -1
Query: 419 IQPHKGVIVALHDVICSDIVHPVLTYRNRFQNH*LIPVDERQNDYVSKILCFLLQCCIYL 240
I P + +I+ H V+C D HP+L+ N Q++ +I +++R + V + LC LL+ CI
Sbjct: 533 IDPDECIIITFHYVLCRDNAHPILSNGNGLQHNKVIAINKRHYNNVLEELCLLLELCINF 354
Query: 239 LSVAQRNKEPSNRI 198
L Q +KEPS I
Sbjct: 353 LFSIQSHKEPSYNI 312
>gb|CF489000.1|CF489000 POL1_53_C07.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_53_C07_A002 5', mRNA sequence
Length = 669
Score = 91.3 bits (193), Expect(3) = 6e-047
Identities = 35/55 (63%), Positives = 46/55 (83%)
Frame = +3 / +3
Query: 189 QSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQY 353
+S N VARFFVAL R E+N LKKEAE+FGDIV +P++D Y+LVV+KT+AIC++
Sbjct: 375 KSPNRVARFFVALHGRNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEF 539
Score = 87.2 bits (184), Expect(3) = 6e-047
Identities = 33/57 (57%), Positives = 41/57 (71%)
Frame = +3 / +3
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWM 173
A +LP HPSF Q LE S W+A +P+EP+ +FIGILS+ NHFAERM +R TWM
Sbjct: 192 AGSLPTTHPSFEPQNYLEFSTVWQAPPLPDEPVEIFIGILSSANHFAERMGVRTTWM 362
Score = 47.8 bits (98), Expect(3) = 6e-047
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = +3 / +2
Query: 345 CQYGVHNVTADYIMKCDDDTFVRLD 419
C VH V+A Y+MKCDDDTFVR+D
Sbjct: 524 CNMRVHVVSAKYVMKCDDDTFVRID 598
>gb|CW135635.1|CW135635 104_520_11117953_116_34836_009 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11117953, DNA
sequence
Length = 678
Score = 91.8 bits (194), Expect(4) = 8e-045
Identities = 37/57 (64%), Positives = 43/57 (75%)
Frame = +3 / -1
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWM 173
A LP HPSFS Q+ LEM W+A +P+EP+ +FIGILSA NHFAERMA RKTWM
Sbjct: 561 AGTLPTTHPSFSPQKHLEMLPSWQAPPLPDEPVEIFIGILSAGNHFAERMAARKTWM 391
Score = 82.2 bits (173), Expect(4) = 8e-045
Identities = 31/42 (73%), Positives = 38/42 (90%)
Frame = +3 / -3
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
R E+N LKKEAE+FGDIVI+PF+D Y+LVVLKT+AIC+YGV
Sbjct: 262 RNEVNVELKKEAEFFGDIVIVPFMDSYDLVVLKTIAICEYGV 137
Score = 37.3 bits (75), Expect(4) = 8e-045
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = +3 / -1
Query: 357 VHNVTADYIMKCDDDTF 407
VH V+A YIMKCDDDTF
Sbjct: 51 VHVVSARYIMKCDDDTF 1
Score = 25.4 bits (49), Expect(4) = 8e-045
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = +3 / -1
Query: 183 AIQSGNAVARFFVAL 227
A +S N VARFFVAL
Sbjct: 384 AQKSSNVVARFFVAL 340
>gb|CW241144.1|CW241144 104_700_11218605_148_37542_093 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11218605, DNA
sequence
Length = 718
Score = 85.8 bits (181), Expect(4) = 1e-041
Identities = 33/57 (57%), Positives = 44/57 (77%)
Frame = +3 / -1
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWM 173
A +LP + P+ + + LEMSE+WKA +P EP LFIGILS+ NHFAERMA+RK+W+
Sbjct: 607 AGSLPTSPPTSATKSYLEMSEQWKASPLPTEPAELFIGILSSANHFAERMAVRKSWL 437
Score = 80.8 bits (170), Expect(4) = 1e-041
Identities = 31/45 (68%), Positives = 39/45 (86%)
Frame = +3 / -3
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVHNV 368
R E+N LKKEA+YFGDIVI+PF+D Y+LVVLKT+AI +YGV N+
Sbjct: 305 RNEVNEELKKEADYFGDIVIVPFMDSYDLVVLKTIAIVEYGVRNL 171
Score = 36.8 bits (74), Expect(4) = 1e-041
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +3 / -1
Query: 351 YGVHNVTADYIMKCDDDTFVRLD 419
+ V + A +IMKCDDDTFVR++
Sbjct: 88 FQVRVIPAKHIMKCDDDTFVRIE 20
Score = 22.6 bits (43), Expect(4) = 1e-041
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +3 / -1
Query: 189 QSGNAVARFFVAL 227
+S + VARFFVAL
Sbjct: 424 RSSDVVARFFVAL 386
>gb|CF484299.1|CF484299 POL1_4_A06.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_4_A06_A002 5', mRNA sequence
Length = 567
Score = 138 bits (295), Expect(2) = 1e-038
Identities = 53/77 (68%), Positives = 66/77 (85%)
Frame = +3 / +3
Query: 189 QSGNAVARFFVALSHRKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVHNV 368
+S N VARFFVAL R E+N LKKEAE+FGDIV +P++D Y+LVV+KT+AIC+YGVH V
Sbjct: 75 KSPNRVARFFVALHGRNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYGVHVV 254
Query: 369 TADYIMKCDDDTFVRLD 419
+A Y+MKCDDDTFVR+D
Sbjct: 255 SAKYVMKCDDDTFVRID 305
Score = 42.3 bits (86), Expect(2) = 1e-038
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +3 / +3
Query: 114 GILSATNHFAERMAIRKTWM 173
GILS+ NHFAERM +R TWM
Sbjct: 3 GILSSANHFAERMGVRTTWM 62
>gb|CW437404.1|CW437404 fsbb001f154i03k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f154i03, DNA
sequence
Length = 710
Score = 78.5 bits (165), Expect(4) = 4e-036
Identities = 28/42 (66%), Positives = 37/42 (88%)
Frame = +3 / -1
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
R E+N LKKEAE+FGDIV +P++D Y+LVV+KT+AIC+YGV
Sbjct: 467 RNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYGV 342
Score = 60.2 bits (125), Expect(4) = 4e-036
Identities = 21/34 (61%), Positives = 28/34 (82%)
Frame = +3 / -2
Query: 72 KARTVPEEPIHLFIGILSATNHFAERMAIRKTWM 173
+A +P+EP+ +FIGILS+ NHFAERM +R TWM
Sbjct: 709 QAPPLPDEPVEIFIGILSSANHFAERMGVRTTWM 608
Score = 45.1 bits (92), Expect(4) = 4e-036
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +3 / -2
Query: 357 VHNVTADYIMKCDDDTFVRLD 419
VH V+A Y+MKCDDDTFVR+D
Sbjct: 244 VHVVSAKYVMKCDDDTFVRID 182
Score = 23.5 bits (45), Expect(4) = 4e-036
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +3 / -2
Query: 189 QSGNAVARFFVAL 227
+S N VARFFVAL
Sbjct: 595 KSPNRVARFFVAL 557
>gb|BZ334822.1|BZ334822 hx93g02.b1 WGS-SbicolorF (JM107 adapted methyl filtered) Sorghum
bicolor genomic clone hx93g02 5', DNA sequence
Length = 749
Score = 87.2 bits (184), Expect(3) = 2e-035
Identities = 33/57 (57%), Positives = 41/57 (71%)
Frame = +3 / -1
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWM 173
A +LP HPSF Q LE S W+A +P+EP+ +FIGILS+ NHFAERM +R TWM
Sbjct: 434 AGSLPTTHPSFEPQNYLEFSTVWQAPPLPDEPVEIFIGILSSANHFAERMGVRTTWM 264
Score = 76.7 bits (161), Expect(3) = 2e-035
Identities = 27/41 (65%), Positives = 36/41 (87%)
Frame = +3 / -3
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYG 356
R E+N LKKEAE+FGDIV +P++D Y+LVV+KT+AIC+YG
Sbjct: 123 RNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYG 1
Score = 23.5 bits (45), Expect(3) = 2e-035
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +3 / -1
Query: 189 QSGNAVARFFVAL 227
+S N VARFFVAL
Sbjct: 251 KSPNRVARFFVAL 213
>gb|CW042123.1|CW042123 104_276_10506948_114_30476 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10506948, DNA
sequence
Length = 711
Score = 78.5 bits (165), Expect(4) = 1e-032
Identities = 28/42 (66%), Positives = 37/42 (88%)
Frame = +3 / -3
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
R E+N LKKEAE+FGDIV +P++D Y+LVV+KT+AIC+YGV
Sbjct: 502 RNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYGV 377
Score = 47.8 bits (98), Expect(4) = 1e-032
Identities = 17/23 (73%), Positives = 20/23 (86%)
Frame = +3 / -1
Query: 105 LFIGILSATNHFAERMAIRKTWM 173
+FIGILS+ NHFAERM +R TWM
Sbjct: 711 IFIGILSSANHFAERMGVRTTWM 643
Score = 45.1 bits (92), Expect(4) = 1e-032
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +3 / -1
Query: 357 VHNVTADYIMKCDDDTFVRLD 419
VH V+A Y+MKCDDDTFVR+D
Sbjct: 279 VHVVSAKYVMKCDDDTFVRID 217
Score = 23.5 bits (45), Expect(4) = 1e-032
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +3 / -1
Query: 189 QSGNAVARFFVAL 227
+S N VARFFVAL
Sbjct: 630 KSPNRVARFFVAL 592
>gb|CL700967.2|CL700967 SP__Ba0067G06.r SP__Ba Sorghum propinquum genomic clone
SP__Ba0067G06 3', DNA sequence
Length = 442
Score = 78.5 bits (165), Expect(3) = 2e-032
Identities = 28/42 (66%), Positives = 37/42 (88%)
Frame = +3 / +1
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
R E+N LKKEAE+FGDIV +P++D Y+LVV+KT+AIC+YGV
Sbjct: 280 RNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYGV 405
Score = 73.9 bits (155), Expect(3) = 2e-032
Identities = 27/44 (61%), Positives = 34/44 (77%)
Frame = +3 / +2
Query: 42 QQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWM 173
Q LE S W+A +P+EP+ +FIGILS+ NHFAERM +RKTWM
Sbjct: 8 QNYLEFSTVWQAPPLPDEPVEIFIGILSSANHFAERMGVRKTWM 139
Score = 23.5 bits (45), Expect(3) = 2e-032
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +3 / +2
Query: 189 QSGNAVARFFVAL 227
+S N VARFFVAL
Sbjct: 152 KSPNRVARFFVAL 190
>gb|CW098901.1|CW098901 104_465_11003259_116_34374_006 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11003259, DNA
sequence
Length = 610
Score = 91.8 bits (194), Expect(3) = 5e-029
Identities = 37/57 (64%), Positives = 43/57 (75%)
Frame = +3 / -2
Query: 3 ATALPKAHPSFSLQQVLEMSERWKARTVPEEPIHLFIGILSATNHFAERMAIRKTWM 173
A LP HPSFS Q+ LEM W+A +P+EP+ +FIGILSA NHFAERMA RKTWM
Sbjct: 375 AGTLPTTHPSFSPQKHLEMLPSWQAPPLPDEPVEIFIGILSAGNHFAERMAARKTWM 205
Score = 48.7 bits (100), Expect(3) = 5e-029
Identities = 18/25 (72%), Positives = 22/25 (88%)
Frame = +3 / -1
Query: 234 RKEINAALKKEAEYFGDIVILPFID 308
R E+N LKKEAE+FGDIVI+PF+D
Sbjct: 76 RNEVNVELKKEAEFFGDIVIVPFMD 2
Score = 25.4 bits (49), Expect(3) = 5e-029
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = +3 / -2
Query: 183 AIQSGNAVARFFVAL 227
A +S N VARFFVAL
Sbjct: 198 AQKSSNVVARFFVAL 154
>gb|CW111980.1|CW111980 104_485_11104632_116_34538_086 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11104632, DNA
sequence
Length = 621
Score = 78.5 bits (165), Expect(3) = 2e-026
Identities = 28/42 (66%), Positives = 37/42 (88%)
Frame = +3 / -2
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
R E+N LKKEAE+FGDIV +P++D Y+LVV+KT+AIC+YGV
Sbjct: 434 RNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYGV 309
Score = 45.1 bits (92), Expect(3) = 2e-026
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +3 / -3
Query: 357 VHNVTADYIMKCDDDTFVRLD 419
VH V+A Y+MKCDDDTFVR+D
Sbjct: 211 VHVVSAKYVMKCDDDTFVRID 149
Score = 33.6 bits (67), Expect(3) = 2e-026
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = +3 / -3
Query: 132 NHFAERMAIRKTWM 173
NHFAERM +R TWM
Sbjct: 616 NHFAERMGVRTTWM 575
>gb|CW241143.1|CW241143 104_700_11218605_116_37545_093 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11218605, DNA
sequence
Length = 435
Score = 80.8 bits (170), Expect(3) = 6e-026
Identities = 31/45 (68%), Positives = 39/45 (86%)
Frame = +3 / -1
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGVHNV 368
R E+N LKKEA+YFGDIVI+PF+D Y+LVVLKT+AI +YGV N+
Sbjct: 231 RNEVNEELKKEADYFGDIVIVPFMDSYDLVVLKTIAIVEYGVRNL 97
Score = 50.1 bits (103), Expect(3) = 6e-026
Identities = 18/23 (78%), Positives = 22/23 (95%)
Frame = +3 / -2
Query: 105 LFIGILSATNHFAERMAIRKTWM 173
LFIGILS+ NHFAERMA+RK+W+
Sbjct: 431 LFIGILSSANHFAERMAVRKSWL 363
Score = 22.6 bits (43), Expect(3) = 6e-026
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +3 / -2
Query: 189 QSGNAVARFFVAL 227
+S + VARFFVAL
Sbjct: 350 RSSDVVARFFVAL 312
>gb|CN127712.1|CN127712 RHOH1_24_D05.g3_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_24_D05_A002 5', mRNA sequence
Length = 844
Score = 114 bits (244), Expect = 6e-025
Identities = 45/49 (91%), Positives = 48/49 (97%)
Frame = +3 / +2
Query: 276 FGDIVILPFIDRYELVVLKTVAICQYGVHNVTADYIMKCDDDTFVRLDI 422
FGDIVILPF+DRYELVVLKTVA+CQYGV NVTADYIMKCDDDTFVRLD+
Sbjct: 2 FGDIVILPFMDRYELVVLKTVALCQYGVQNVTADYIMKCDDDTFVRLDV 148
Score = 101 bits (215), Expect = 6e-021
Identities = 44/49 (89%), Positives = 45/49 (91%)
Frame = -1 / -2
Query: 424 TISSRTKVSSSHFMM*SAVTLCTPY*HIATVFRTTSSYRSMNGRMTMSP 278
T SS TKVSSSHFMM*SAVT CTPY*H ATVFRTTSSYRS+NGRMTMSP
Sbjct: 150 TTSSLTKVSSSHFMM*SAVTFCTPY*HSATVFRTTSSYRSINGRMTMSP 4
>gb|CW209770.1|CW209770 104_640_11189431_116_37430_032 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11189431, DNA
sequence
Length = 666
Score = 82.2 bits (173), Expect(2) = 4e-023
Identities = 31/42 (73%), Positives = 38/42 (90%)
Frame = +3 / -2
Query: 234 RKEINAALKKEAEYFGDIVILPFIDRYELVVLKTVAICQYGV 359
R E+N LKKEAE+FGDIVI+PF+D Y+LVVLKT+AIC+YGV
Sbjct: 602 RNEVNVELKKEAEFFGDIVIVPFMDSYDLVVLKTIAICEYGV 477
Score = 46.0 bits (94), Expect(2) = 4e-023
Identities = 18/21 (85%), Positives = 19/21 (90%)
Frame = +3 / -3
Query: 357 VHNVTADYIMKCDDDTFVRLD 419
VH V+A YIMKCDDDTFVRLD
Sbjct: 391 VHVVSARYIMKCDDDTFVRLD 329
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 576,257,619
Number of Sequences: 832831
Number of extensions: 8294874
Number of successful extensions: 211431
Number of sequences better than 1.0e-020: 16
length of database: 163,786,556
effective HSP length: 52
effective length of database: 120,479,344
effective search space used: 10602182272
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)