TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAS4c05.yg.2.1
(318 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW111186.1|CW111186 104_484_11104164_116_34532_042 Sorgh... 149 2e-035
gb|CW111187.1|CW111187 104_484_11104164_148_34528_042 Sorgh... 149 2e-035
gb|CW274351.1|CW274351 104_747_11404381_148_35370_059 Sorgh... 149 2e-035
>gb|CW111186.1|CW111186 104_484_11104164_116_34532_042 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11104164, DNA
sequence
Length = 653
Score = 149 bits (319), Expect = 2e-035
Identities = 64/79 (81%), Positives = 64/79 (81%)
Frame = +2 / +1
Query: 2 HLAAAATDEGVEVGXWDLAELQKLECLGQRHGXXQREP*DLEHLGVRSDXXENFLDHLXH 181
HLAA ATDEGVEVG WDLAELQKLECLGQRH QREP*DL HLGVR D ENFLDHL H
Sbjct: 178 HLAATATDEGVEVGRWDLAELQKLECLGQRHRS*QREP*DLGHLGVRPDCSENFLDHLQH 357
Query: 182 LLASTXXXXVAXHSYDEQA 238
LLAST VA HSYDEQA
Sbjct: 358 LLASTAIVFVAVHSYDEQA 414
Score = 145 bits (311), Expect = 2e-034
Identities = 63/79 (79%), Positives = 66/79 (83%)
Frame = +3 / +2
Query: 3 TSLPLPQMRASKSXAGILPSCRSWSVSDNGMAXDSVNPEILNIWESDRTXLKTSXITCXI 182
TSLPLPQMRASKS AGILPS RSWSVSDNG+A DS NPEIL+IWESDRT LKTS ITC I
Sbjct: 179 TSLPLPQMRASKSVAGILPSFRSWSVSDNGIAVDSANPEILDIWESDRTALKTSWITCSI 358
Query: 183 FLLAXXXXX*QXTAMTNKR 239
FLLA *Q TAMTN+R
Sbjct: 359 FLLARPSSS*QSTAMTNRR 415
Score = 141 bits (303), Expect = 3e-033
Identities = 64/79 (81%), Positives = 65/79 (82%)
Frame = -3 / -3
Query: 238 RLFVIAVXCYXXXXXASKKMXQVIXEVFXXVRSDSQMFKISGFTLSXAMPLSETLQLLQL 59
RLFVIAV CY ASKKM QVI EVF VRSDSQM KISGF LS AMPLSETLQLL+L
Sbjct: 414 RLFVIAVDCYEDDGRASKKMLQVIQEVFRAVRSDSQMSKISGFALSTAMPLSETLQLLKL 235
Query: 58 GKIPAXDFDALICGSGSEV 2
GKIPA DFDALICGSGSEV
Sbjct: 234 GKIPATDFDALICGSGSEV 178
Score = 117 bits (250), Expect = 6e-026
Identities = 52/77 (67%), Positives = 53/77 (68%)
Frame = -2 / -2
Query: 233 VRHSCXLLXXXXXC*QEDXAGDXGSFQXSPIGLPDVQDLXXXXXXXXXXXRDTPASAARQ 54
VRHSC LL C*QED AGD SFQ SP+GLPDVQDL RDTPAS ARQ
Sbjct: 409 VRHSCGLLRRRWPC*QEDAAGDPRSFQSSPVGLPDVQDLRVRAVNCDAVVRDTPASEARQ 230
Query: 53 DPSXRLRRPHLWQRQRG 3
DPS RLRRPHLWQ QRG
Sbjct: 229 DPSDRLRRPHLWQWQRG 179
>gb|CW111187.1|CW111187 104_484_11104164_148_34528_042 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11104164, DNA
sequence
Length = 646
Score = 149 bits (319), Expect = 2e-035
Identities = 64/79 (81%), Positives = 64/79 (81%)
Frame = +2 / -2
Query: 2 HLAAAATDEGVEVGXWDLAELQKLECLGQRHGXXQREP*DLEHLGVRSDXXENFLDHLXH 181
HLAA ATDEGVEVG WDLAELQKLECLGQRH QREP*DL HLGVR D ENFLDHL H
Sbjct: 612 HLAATATDEGVEVGRWDLAELQKLECLGQRHRS*QREP*DLGHLGVRPDCSENFLDHLQH 433
Query: 182 LLASTXXXXVAXHSYDEQA 238
LLAST VA HSYDEQA
Sbjct: 432 LLASTAIVFVAVHSYDEQA 376
Score = 145 bits (311), Expect = 2e-034
Identities = 63/79 (79%), Positives = 66/79 (83%)
Frame = +3 / -3
Query: 3 TSLPLPQMRASKSXAGILPSCRSWSVSDNGMAXDSVNPEILNIWESDRTXLKTSXITCXI 182
TSLPLPQMRASKS AGILPS RSWSVSDNG+A DS NPEIL+IWESDRT LKTS ITC I
Sbjct: 611 TSLPLPQMRASKSVAGILPSFRSWSVSDNGIAVDSANPEILDIWESDRTALKTSWITCSI 432
Query: 183 FLLAXXXXX*QXTAMTNKR 239
FLLA *Q TAMTN+R
Sbjct: 431 FLLARPSSS*QSTAMTNRR 375
Score = 141 bits (303), Expect = 3e-033
Identities = 64/79 (81%), Positives = 65/79 (82%)
Frame = -3 / +1
Query: 238 RLFVIAVXCYXXXXXASKKMXQVIXEVFXXVRSDSQMFKISGFTLSXAMPLSETLQLLQL 59
RLFVIAV CY ASKKM QVI EVF VRSDSQM KISGF LS AMPLSETLQLL+L
Sbjct: 376 RLFVIAVDCYEDDGRASKKMLQVIQEVFRAVRSDSQMSKISGFALSTAMPLSETLQLLKL 555
Query: 58 GKIPAXDFDALICGSGSEV 2
GKIPA DFDALICGSGSEV
Sbjct: 556 GKIPATDFDALICGSGSEV 612
Score = 117 bits (250), Expect = 6e-026
Identities = 52/77 (67%), Positives = 53/77 (68%)
Frame = -2 / +3
Query: 233 VRHSCXLLXXXXXC*QEDXAGDXGSFQXSPIGLPDVQDLXXXXXXXXXXXRDTPASAARQ 54
VRHSC LL C*QED AGD SFQ SP+GLPDVQDL RDTPAS ARQ
Sbjct: 381 VRHSCGLLRRRWPC*QEDAAGDPRSFQSSPVGLPDVQDLRVRAVNCDAVVRDTPASEARQ 560
Query: 53 DPSXRLRRPHLWQRQRG 3
DPS RLRRPHLWQ QRG
Sbjct: 561 DPSDRLRRPHLWQWQRG 611
>gb|CW274351.1|CW274351 104_747_11404381_148_35370_059 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11404381, DNA
sequence
Length = 712
Score = 149 bits (319), Expect = 2e-035
Identities = 64/79 (81%), Positives = 64/79 (81%)
Frame = +2 / +2
Query: 2 HLAAAATDEGVEVGXWDLAELQKLECLGQRHGXXQREP*DLEHLGVRSDXXENFLDHLXH 181
HLAA ATDEGVEVG WDLAELQKLECLGQRH QREP*DL HLGVR D ENFLDHL H
Sbjct: 332 HLAATATDEGVEVGRWDLAELQKLECLGQRHRS*QREP*DLGHLGVRPDCSENFLDHLQH 511
Query: 182 LLASTXXXXVAXHSYDEQA 238
LLAST VA HSYDEQA
Sbjct: 512 LLASTAIVFVAVHSYDEQA 568
Score = 145 bits (311), Expect = 2e-034
Identities = 63/79 (79%), Positives = 66/79 (83%)
Frame = +3 / +3
Query: 3 TSLPLPQMRASKSXAGILPSCRSWSVSDNGMAXDSVNPEILNIWESDRTXLKTSXITCXI 182
TSLPLPQMRASKS AGILPS RSWSVSDNG+A DS NPEIL+IWESDRT LKTS ITC I
Sbjct: 333 TSLPLPQMRASKSVAGILPSFRSWSVSDNGIAVDSANPEILDIWESDRTALKTSWITCSI 512
Query: 183 FLLAXXXXX*QXTAMTNKR 239
FLLA *Q TAMTN+R
Sbjct: 513 FLLARPSSS*QSTAMTNRR 569
Score = 141 bits (303), Expect = 3e-033
Identities = 64/79 (81%), Positives = 65/79 (82%)
Frame = -3 / -1
Query: 238 RLFVIAVXCYXXXXXASKKMXQVIXEVFXXVRSDSQMFKISGFTLSXAMPLSETLQLLQL 59
RLFVIAV CY ASKKM QVI EVF VRSDSQM KISGF LS AMPLSETLQLL+L
Sbjct: 568 RLFVIAVDCYEDDGRASKKMLQVIQEVFRAVRSDSQMSKISGFALSTAMPLSETLQLLKL 389
Query: 58 GKIPAXDFDALICGSGSEV 2
GKIPA DFDALICGSGSEV
Sbjct: 388 GKIPATDFDALICGSGSEV 332
Score = 117 bits (250), Expect = 6e-026
Identities = 52/77 (67%), Positives = 53/77 (68%)
Frame = -2 / -3
Query: 233 VRHSCXLLXXXXXC*QEDXAGDXGSFQXSPIGLPDVQDLXXXXXXXXXXXRDTPASAARQ 54
VRHSC LL C*QED AGD SFQ SP+GLPDVQDL RDTPAS ARQ
Sbjct: 563 VRHSCGLLRRRWPC*QEDAAGDPRSFQSSPVGLPDVQDLRVRAVNCDAVVRDTPASEARQ 384
Query: 53 DPSXRLRRPHLWQRQRG 3
DPS RLRRPHLWQ QRG
Sbjct: 383 DPSDRLRRPHLWQWQRG 333
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 190,647,424
Number of Sequences: 832831
Number of extensions: 1316531
Number of successful extensions: 101437
Number of sequences better than 1.0e-020: 3
length of database: 163,786,556
effective HSP length: 51
effective length of database: 121,312,175
effective search space used: 6550857450
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)