TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF14f01.yg.2.1
(319 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW306008.1|CW306008 104_791_11466607_148_36273_024 Sorgh... 80 3e-029
gb|CN140420.1|CN140420 OX1_36_B10.b1_A002 Oxidatively-stres... 74 2e-024
gb|CN149194.1|CN149194 WOUND1_61_F10.b1_A002 Wounded leaves... 74 5e-024
gb|CL158360.1|CL158360 104_347_10803488_114_31377_080 Sorgh... 110 9e-024
gb|CL158361.1|CL158361 104_347_10803488_116_31378_080 Sorgh... 110 9e-024
gb|CF070980.1|CF070980 FE1_14_F12.g1_A002 Iron-deficient se... 62 7e-021
>gb|CW306008.1|CW306008 104_791_11466607_148_36273_024 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11466607, DNA
sequence
Length = 675
Score = 79.9 bits (168), Expect(2) = 3e-029
Identities = 32/52 (61%), Positives = 34/52 (65%)
Frame = -3 / -1
Query: 176 NEDXELWDPXSILTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
NE+ LWDP S+L G G TK DKSWHVMGLGYNP I PE I AAV
Sbjct: 288 NENGTLWDPTSVLPVGLMTFYGKTKPLDKSWHVMGLGYNPHIRPEDIGGAAV 133
Score = 67.5 bits (141), Expect(2) = 3e-029
Identities = 24/47 (51%), Positives = 33/47 (70%)
Frame = -3 / -3
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEM 177
YL P+V++ F+ ACAW+YGVNVF L R+ CT+ FH++MEM
Sbjct: 538 YLNFSEPVVQESFSHRACAWSYGVNVFDLQGWRREQCTQQFHRFMEM 398
>gb|CN140420.1|CN140420 OX1_36_B10.b1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_36_B10_A002 3', mRNA sequence
Length = 836
Score = 74.4 bits (156), Expect(2) = 2e-024
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +3
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ +FNPNAC WAYG+N F L R+ CTE +H + NE+ LW
Sbjct: 237 YMNFSHPLIKAKFNPNACGWAYGMNFFDLDSWRREKCTEQYHYWQNQNENRTLW 398
Score = 57.4 bits (119), Expect(2) = 2e-024
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / +3
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK +KSWHV+GLGYNPSIS E IR+AAV
Sbjct: 411 LPPGLITFYSTTKPLEKSWHVLGLGYNPSISMEEIRNAAV 530
>gb|CN149194.1|CN149194 WOUND1_61_F10.b1_A002 Wounded leaves Sorghum bicolor cDNA clone
WOUND1_61_F10_A002 3', mRNA sequence
Length = 807
Score = 74.4 bits (156), Expect(2) = 5e-024
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +2
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ +FNPNAC WAYG+N F L R+ CTE +H + NE+ LW
Sbjct: 134 YMNFSHPLIKAKFNPNACGWAYGMNFFDLDSWRREKCTEQYHYWQNQNENRTLW 295
Score = 56.1 bits (116), Expect(2) = 5e-024
Identities = 23/40 (57%), Positives = 27/40 (67%)
Frame = -3 / +2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK ++SWHV+GLGYNPSIS E IR+AAV
Sbjct: 308 LPPGLITFYSTTKPLERSWHVLGLGYNPSISMEEIRNAAV 427
>gb|CL158360.1|CL158360 104_347_10803488_114_31377_080 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10803488, DNA
sequence
Length = 727
Score = 110 bits (234), Expect = 9e-024
Identities = 42/54 (77%), Positives = 42/54 (77%)
Frame = +2 / -3
Query: 17 VPLQHYGLPQG*CLDYSQDPLHAMTYPXAWYCXXXXXXQLLEWXXDPTAXXPHS 178
V LQHYGLPQG*CLDYSQDPLHAMTYP AWYC QLLEW DPTA PHS
Sbjct: 593 VSLQHYGLPQG*CLDYSQDPLHAMTYPVAWYCHRRTSAQLLEWM*DPTARHPHS 432
Score = 105 bits (225), Expect = 2e-022
Identities = 43/56 (76%), Positives = 45/56 (80%)
Frame = -3 / +2
Query: 188 YMEMNEDXELWDPXSILTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
++ NED ELWDP SILTAG GNTK DKSWHVMGLGYNPSISPEAIRSAAV
Sbjct: 422 FLWQNEDGELWDPTSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 589
Score = 101 bits (215), Expect = 4e-021
Identities = 43/54 (79%), Positives = 43/54 (79%)
Frame = -2 / +1
Query: 177 E*GXXAVGSXIHSNSWXXXXXXQYQAXG*VMACNGSWL*SKHQP*GNP*CCSGT 16
E*G AVGS IHSNSW QYQA G*VMACNGSWL*SKHQP*GNP*CCS T
Sbjct: 433 E*GWRAVGSYIHSNSWADVLLWQYQATG*VMACNGSWL*SKHQP*GNP*CCSDT 594
>gb|CL158361.1|CL158361 104_347_10803488_116_31378_080 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10803488, DNA
sequence
Length = 731
Score = 110 bits (234), Expect = 9e-024
Identities = 42/54 (77%), Positives = 42/54 (77%)
Frame = +2 / +3
Query: 17 VPLQHYGLPQG*CLDYSQDPLHAMTYPXAWYCXXXXXXQLLEWXXDPTAXXPHS 178
V LQHYGLPQG*CLDYSQDPLHAMTYP AWYC QLLEW DPTA PHS
Sbjct: 243 VSLQHYGLPQG*CLDYSQDPLHAMTYPVAWYCHRRTSAQLLEWM*DPTARHPHS 404
Score = 105 bits (225), Expect = 2e-022
Identities = 43/56 (76%), Positives = 45/56 (80%)
Frame = -3 / -3
Query: 188 YMEMNEDXELWDPXSILTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
++ NED ELWDP SILTAG GNTK DKSWHVMGLGYNPSISPEAIRSAAV
Sbjct: 414 FLWQNEDGELWDPTSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 247
Score = 101 bits (215), Expect = 4e-021
Identities = 43/54 (79%), Positives = 43/54 (79%)
Frame = -2 / -2
Query: 177 E*GXXAVGSXIHSNSWXXXXXXQYQAXG*VMACNGSWL*SKHQP*GNP*CCSGT 16
E*G AVGS IHSNSW QYQA G*VMACNGSWL*SKHQP*GNP*CCS T
Sbjct: 403 E*GWRAVGSYIHSNSWADVLLWQYQATG*VMACNGSWL*SKHQP*GNP*CCSDT 242
>gb|CF070980.1|CF070980 FE1_14_F12.g1_A002 Iron-deficient seedlings Sorghum bicolor cDNA
clone FE1_14_F12_A002 5', mRNA sequence
Length = 692
Score = 61.6 bits (128), Expect(2) = 7e-021
Identities = 21/46 (45%), Positives = 29/46 (63%)
Frame = -3 / -1
Query: 293 VRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
++ +FNPNAC AYG+N F L R+ CTE +H + NE+ LW
Sbjct: 692 IKAKFNPNACGGAYGMNFFDLDSWRREKCTEQYHYWQNQNENRTLW 555
Score = 57.4 bits (119), Expect(2) = 7e-021
Identities = 24/40 (60%), Positives = 27/40 (67%)
Frame = -3 / -1
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK +KSWHV+GLGYNPSIS E IR+AAV
Sbjct: 542 LPPGLITFYSTTKPLEKSWHVLGLGYNPSISMEEIRNAAV 423
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 347,495,375
Number of Sequences: 832831
Number of extensions: 3551182
Number of successful extensions: 245003
Number of sequences better than 1.0e-020: 7
length of database: 163,786,556
effective HSP length: 51
effective length of database: 121,312,175
effective search space used: 6550857450
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)