TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF11h01.xg.2.1
(328 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW306008.1|CW306008 104_791_11466607_148_36273_024 Sorgh... 104 8e-045
gb|CN140420.1|CN140420 OX1_36_B10.b1_A002 Oxidatively-stres... 100 3e-037
gb|CN149194.1|CN149194 WOUND1_61_F10.b1_A002 Wounded leaves... 100 8e-037
gb|CL158360.1|CL158360 104_347_10803488_114_31377_080 Sorgh... 133 9e-031
gb|CL158361.1|CL158361 104_347_10803488_116_31378_080 Sorgh... 133 9e-031
gb|CF070980.1|CF070980 FE1_14_F12.g1_A002 Iron-deficient se... 75 9e-030
gb|CN127460.1|CN127460 RHOH1_23_D06.b1_A002 Acid- and alkal... 86 5e-029
gb|CX612893.1|CX612893 GABR1_5_C04.b1_A002 GA- or brassinol... 86 5e-029
gb|CF487333.1|CF487333 POL1_43_D11.b1_A002 Pollen Sorghum b... 79 3e-028
gb|CW144377.1|CW144377 104_536_11137911_148_34962_056 Sorgh... 70 7e-026
gb|BF587532.1|BF587532 FM1_37_E11.g1_A003 Floral-Induced Me... 75 2e-024
>gb|CW306008.1|CW306008 104_791_11466607_148_36273_024 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11466607, DNA
sequence
Length = 675
Score = 104 bits (221), Expect(2) = 8e-045
Identities = 40/52 (76%), Positives = 43/52 (82%)
Frame = -3 / -1
Query: 158 NEDGELWDPTSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
NE+G LWDPTS+L GLM+FYG TKPLDKSWHVMGLGYNP I PE I AAV
Sbjct: 288 NENGTLWDPTSVLPVGLMTFYGKTKPLDKSWHVMGLGYNPHIRPEDIGGAAV 133
Score = 95.5 bits (202), Expect(2) = 8e-045
Identities = 32/51 (62%), Positives = 44/51 (86%)
Frame = -3 / -3
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEM 159
RY KYLNF++P+V++ F+ ACAW+YGVNVFDL+ WRR+ CT+ FH++MEM
Sbjct: 550 RYGKYLNFSEPVVQESFSHRACAWSYGVNVFDLQGWRREQCTQQFHRFMEM 398
Score = 66.1 bits (138), Expect(2) = 4e-022
Identities = 30/44 (68%), Positives = 33/44 (75%)
Frame = +1 / +2
Query: 4 TAALRIASGLMLGL*PRPITCHDLSSGLVLP*KDISPAVRMDVG 135
TAA ++SGLM GL*P PITCHDLSSG VLP* +SP DVG
Sbjct: 134 TAAPPMSSGLMCGL*PSPITCHDLSSGFVLP*NVMSPTGSTDVG 265
Score = 57.4 bits (119), Expect(2) = 4e-022
Identities = 24/51 (47%), Positives = 33/51 (64%)
Frame = +3 / +2
Query: 159 HFHILMEQFCATIPPPCLKVEHIYPICPCTGIGIESIPHNGLREVEILPVP 311
HFH +E A +P P L+VEH+ + P G E++ H+GLREVE+L VP
Sbjct: 398 HFHESVELLGALLPAPPLEVEHVDAVGPGAGAVAEALLHDGLREVEVLAVP 550
>gb|CN140420.1|CN140420 OX1_36_B10.b1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_36_B10_A002 3', mRNA sequence
Length = 836
Score = 100 bits (213), Expect(2) = 3e-037
Identities = 31/58 (53%), Positives = 45/58 (77%)
Frame = -3 / +3
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
RY +Y+NF+ P+++ +FNPNAC WAYG+N FDL++WRR+ CTE +H + NE+ LW
Sbjct: 225 RYAQYMNFSHPLIKAKFNPNACGWAYGMNFFDLDSWRREKCTEQYHYWQNQNENRTLW 398
Score = 74.4 bits (156), Expect(2) = 3e-037
Identities = 29/40 (72%), Positives = 34/40 (85%)
Frame = -3 / +3
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++FY TKPL+KSWHV+GLGYNPSIS E IR+AAV
Sbjct: 411 LPPGLITFYSTTKPLEKSWHVLGLGYNPSISMEEIRNAAV 530
>gb|CN149194.1|CN149194 WOUND1_61_F10.b1_A002 Wounded leaves Sorghum bicolor cDNA clone
WOUND1_61_F10_A002 3', mRNA sequence
Length = 807
Score = 100 bits (213), Expect(2) = 8e-037
Identities = 31/58 (53%), Positives = 45/58 (77%)
Frame = -3 / +2
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
RY +Y+NF+ P+++ +FNPNAC WAYG+N FDL++WRR+ CTE +H + NE+ LW
Sbjct: 122 RYAQYMNFSHPLIKAKFNPNACGWAYGMNFFDLDSWRREKCTEQYHYWQNQNENRTLW 295
Score = 73.0 bits (153), Expect(2) = 8e-037
Identities = 28/40 (70%), Positives = 34/40 (85%)
Frame = -3 / +2
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++FY TKPL++SWHV+GLGYNPSIS E IR+AAV
Sbjct: 308 LPPGLITFYSTTKPLERSWHVLGLGYNPSISMEEIRNAAV 427
>gb|CL158360.1|CL158360 104_347_10803488_114_31377_080 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10803488, DNA
sequence
Length = 727
Score = 133 bits (285), Expect = 9e-031
Identities = 50/52 (96%), Positives = 51/52 (98%)
Frame = +2 / -3
Query: 5 LQHYGLPQG*CLDYSQDPLHAMTYPVAWYCRKRTSAQLLEWM*DPTARHPHS 160
LQHYGLPQG*CLDYSQDPLHAMTYPVAWYC +RTSAQLLEWM*DPTARHPHS
Sbjct: 587 LQHYGLPQG*CLDYSQDPLHAMTYPVAWYCHRRTSAQLLEWM*DPTARHPHS 432
Score = 131 bits (281), Expect = 3e-030
Identities = 52/56 (92%), Positives = 54/56 (96%)
Frame = -3 / +2
Query: 170 YMEMNEDGELWDPTSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
++ NEDGELWDPTSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV
Sbjct: 422 FLWQNEDGELWDPTSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 589
Score = 130 bits (278), Expect = 8e-030
Identities = 50/52 (96%), Positives = 52/52 (100%)
Frame = +3 / -1
Query: 3 HCSTTDCLRADAWIIAKTHYMP*LIQWLGIAVKGHQPSC*NGCRIPQLAILI 158
HCSTTDCLRADAWIIAKTHYMP*LIQWLGIA++GHQPSC*NGCRIPQLAILI
Sbjct: 589 HCSTTDCLRADAWIIAKTHYMP*LIQWLGIAIEGHQPSC*NGCRIPQLAILI 434
Score = 125 bits (268), Expect = 2e-028
Identities = 51/52 (98%), Positives = 51/52 (98%)
Frame = -2 / +1
Query: 159 E*GWRAVGSYIHSNSWADVLLRQYQATG*VMACNGSWL*SKHQP*GNP*CCS 4
E*GWRAVGSYIHSNSWADVLL QYQATG*VMACNGSWL*SKHQP*GNP*CCS
Sbjct: 433 E*GWRAVGSYIHSNSWADVLLWQYQATG*VMACNGSWL*SKHQP*GNP*CCS 588
Score = 115 bits (246), Expect = 2e-025
Identities = 49/51 (96%), Positives = 49/51 (96%)
Frame = -1 / +3
Query: 157 MRMASCGILHPF*QLG*CPFTAIPSHWISHGM*WVLAIIQASALRQSVVLQ 5
MRMASCGILHPF*QLG*CP AIPSHWISHGM*WVLAIIQASALRQSVVLQ
Sbjct: 435 MRMASCGILHPF*QLG*CPSMAIPSHWISHGM*WVLAIIQASALRQSVVLQ 587
Score = 100 bits (213), Expect = 8e-021
Identities = 44/44 (100%), Positives = 44/44 (100%)
Frame = +1 / -2
Query: 4 TAALRIASGLMLGL*PRPITCHDLSSGLVLP*KDISPAVRMDVG 135
TAALRIASGLMLGL*PRPITCHDLSSGLVLP*KDISPAVRMDVG
Sbjct: 588 TAALRIASGLMLGL*PRPITCHDLSSGLVLP*KDISPAVRMDVG 457
>gb|CL158361.1|CL158361 104_347_10803488_116_31378_080 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10803488, DNA
sequence
Length = 731
Score = 133 bits (285), Expect = 9e-031
Identities = 50/52 (96%), Positives = 51/52 (98%)
Frame = +2 / +3
Query: 5 LQHYGLPQG*CLDYSQDPLHAMTYPVAWYCRKRTSAQLLEWM*DPTARHPHS 160
LQHYGLPQG*CLDYSQDPLHAMTYPVAWYC +RTSAQLLEWM*DPTARHPHS
Sbjct: 249 LQHYGLPQG*CLDYSQDPLHAMTYPVAWYCHRRTSAQLLEWM*DPTARHPHS 404
Score = 131 bits (281), Expect = 3e-030
Identities = 52/56 (92%), Positives = 54/56 (96%)
Frame = -3 / -3
Query: 170 YMEMNEDGELWDPTSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
++ NEDGELWDPTSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV
Sbjct: 414 FLWQNEDGELWDPTSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 247
Score = 130 bits (278), Expect = 8e-030
Identities = 50/52 (96%), Positives = 52/52 (100%)
Frame = +3 / +1
Query: 3 HCSTTDCLRADAWIIAKTHYMP*LIQWLGIAVKGHQPSC*NGCRIPQLAILI 158
HCSTTDCLRADAWIIAKTHYMP*LIQWLGIA++GHQPSC*NGCRIPQLAILI
Sbjct: 247 HCSTTDCLRADAWIIAKTHYMP*LIQWLGIAIEGHQPSC*NGCRIPQLAILI 402
Score = 125 bits (268), Expect = 2e-028
Identities = 51/52 (98%), Positives = 51/52 (98%)
Frame = -2 / -2
Query: 159 E*GWRAVGSYIHSNSWADVLLRQYQATG*VMACNGSWL*SKHQP*GNP*CCS 4
E*GWRAVGSYIHSNSWADVLL QYQATG*VMACNGSWL*SKHQP*GNP*CCS
Sbjct: 403 E*GWRAVGSYIHSNSWADVLLWQYQATG*VMACNGSWL*SKHQP*GNP*CCS 248
Score = 115 bits (246), Expect = 2e-025
Identities = 49/51 (96%), Positives = 49/51 (96%)
Frame = -1 / -1
Query: 157 MRMASCGILHPF*QLG*CPFTAIPSHWISHGM*WVLAIIQASALRQSVVLQ 5
MRMASCGILHPF*QLG*CP AIPSHWISHGM*WVLAIIQASALRQSVVLQ
Sbjct: 401 MRMASCGILHPF*QLG*CPSMAIPSHWISHGM*WVLAIIQASALRQSVVLQ 249
Score = 100 bits (213), Expect = 8e-021
Identities = 44/44 (100%), Positives = 44/44 (100%)
Frame = +1 / +2
Query: 4 TAALRIASGLMLGL*PRPITCHDLSSGLVLP*KDISPAVRMDVG 135
TAALRIASGLMLGL*PRPITCHDLSSGLVLP*KDISPAVRMDVG
Sbjct: 248 TAALRIASGLMLGL*PRPITCHDLSSGLVLP*KDISPAVRMDVG 379
>gb|CF070980.1|CF070980 FE1_14_F12.g1_A002 Iron-deficient seedlings Sorghum bicolor cDNA
clone FE1_14_F12_A002 5', mRNA sequence
Length = 692
Score = 74.8 bits (157), Expect(2) = 9e-030
Identities = 24/46 (52%), Positives = 34/46 (73%)
Frame = -3 / -1
Query: 275 VRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
++ +FNPNAC AYG+N FDL++WRR+ CTE +H + NE+ LW
Sbjct: 692 IKAKFNPNACGGAYGMNFFDLDSWRREKCTEQYHYWQNQNENRTLW 555
Score = 74.4 bits (156), Expect(2) = 9e-030
Identities = 29/40 (72%), Positives = 34/40 (85%)
Frame = -3 / -1
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++FY TKPL+KSWHV+GLGYNPSIS E IR+AAV
Sbjct: 542 LPPGLITFYSTTKPLEKSWHVLGLGYNPSISMEEIRNAAV 423
>gb|CN127460.1|CN127460 RHOH1_23_D06.b1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_23_D06_A002 3', mRNA sequence
Length = 814
Score = 86.3 bits (182), Expect(2) = 5e-029
Identities = 27/58 (46%), Positives = 39/58 (67%)
Frame = -3 / +1
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
RY KYLNF+ P + + +P+ C WA+G+N+FDL WR++ T L+H + E N D LW
Sbjct: 55 RYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKENATSLYHYWQEQNSDLLLW 228
Score = 61.1 bits (127), Expect(2) = 5e-029
Identities = 24/43 (55%), Positives = 30/43 (69%)
Frame = -3 / +1
Query: 131 TSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
T L AGL++FYG +PLD+ WHV+GLGY+ I I SAAV
Sbjct: 232 TGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAV 360
>gb|CX612893.1|CX612893 GABR1_5_C04.b1_A002 GA- or brassinolide-treated seedlings Sorghum
bicolor cDNA clone GABR1_5_C04_A002 3', mRNA sequence
Length = 762
Score = 86.3 bits (182), Expect(2) = 5e-029
Identities = 27/58 (46%), Positives = 39/58 (67%)
Frame = -3 / +1
Query: 311 RYRKYLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
RY KYLNF+ P + + +P+ C WA+G+N+FDL WR++ T L+H + E N D LW
Sbjct: 13 RYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKENATSLYHYWQEQNSDLLLW 186
Score = 61.1 bits (127), Expect(2) = 5e-029
Identities = 24/43 (55%), Positives = 30/43 (69%)
Frame = -3 / +1
Query: 131 TSILTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
T L AGL++FYG +PLD+ WHV+GLGY+ I I SAAV
Sbjct: 190 TGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAV 318
>gb|CF487333.1|CF487333 POL1_43_D11.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_43_D11_A002 3', mRNA sequence
Length = 661
Score = 78.5 bits (165), Expect(2) = 3e-028
Identities = 24/52 (46%), Positives = 36/52 (69%)
Frame = -3 / +2
Query: 293 NFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
NF+ P + F+PNAC WAYG+N+FDL W++ T ++H++ +NED LW
Sbjct: 2 NFSNPHIARNFDPNACGWAYGMNIFDLREWKKKDITGIYHKWQNLNEDRALW 157
Score = 65.7 bits (137), Expect(2) = 3e-028
Identities = 27/40 (67%), Positives = 30/40 (75%)
Frame = -3 / +2
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++FY T PLDKSWHV+GLGYNPSI I SAAV
Sbjct: 170 LPPGLLTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDSAAV 289
>gb|CW144377.1|CW144377 104_536_11137911_148_34962_056 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11137911, DNA
sequence
Length = 622
Score = 70.3 bits (147), Expect(2) = 7e-026
Identities = 21/47 (44%), Positives = 33/47 (70%)
Frame = -3 / +2
Query: 299 YLNFTQPIVRDRFNPNACAWAYGVNVFDLETWRRDGCTELFHQYMEM 159
YLNF+ P + F+PNAC WAYG+N+FDL W++ T ++H++ +
Sbjct: 2 YLNFSNPHIARNFDPNACGWAYGMNIFDLREWKKKDITGIYHKWQNL 142
Score = 65.7 bits (137), Expect(2) = 7e-026
Identities = 27/40 (67%), Positives = 30/40 (75%)
Frame = -3 / +2
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++FY T PLDKSWHV+GLGYNPSI I SAAV
Sbjct: 311 LPPGLLTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDSAAV 430
>gb|BF587532.1|BF587532 FM1_37_E11.g1_A003 Floral-Induced Meristem 1 (FM1) Sorghum
propinquum cDNA, mRNA sequence
Length = 632
Score = 74.8 bits (157), Expect(2) = 2e-024
Identities = 29/40 (72%), Positives = 34/40 (85%)
Frame = -3 / +1
Query: 122 LTAGLMSFYGNTKPLDKSWHVMGLGYNPSISPEAIRSAAV 3
L GL++FY TKPL+KSWHV+GLGYNPSIS E IR+AAV
Sbjct: 115 LPXGLITFYSTTKPLEKSWHVLGLGYNPSISMEEIRNAAV 234
Score = 56.1 bits (116), Expect(2) = 2e-024
Identities = 18/34 (52%), Positives = 25/34 (73%)
Frame = -3 / +1
Query: 239 AYGVNVFDLETWRRDGCTELFHQYMEMNEDGELW 138
AYG+N FDL++WRR+ CTE +H + NE+ LW
Sbjct: 1 AYGMNFFDLDSWRREKCTEQYHYWQNQNENRTLW 102
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 495,459,825
Number of Sequences: 832831
Number of extensions: 7949768
Number of successful extensions: 497507
Number of sequences better than 1.0e-020: 14
length of database: 163,786,556
effective HSP length: 51
effective length of database: 121,312,175
effective search space used: 6914793975
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)