TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5735869.2.1
(539 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE600246.1|BE600246 PI1_94_B03.g1_A002 Pathogen induced ... 100 1e-047
gb|BE358844.1|BE358844 DG1_32_D02.g1_A002 Dark Grown 1 (DG1... 100 5e-044
gb|CW176738.1|CW176738 104_589_11158647_148_36587_052 Sorgh... 67 2e-026
>gb|BE600246.1|BE600246 PI1_94_B03.g1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA,
mRNA sequence
Length = 526
Score = 99.6 bits (211), Expect(5) = 1e-047
Identities = 42/51 (82%), Positives = 43/51 (84%)
Frame = +3 / -1
Query: 387 ITVTIKATDHLRYLRICPDEIIYCSHSFXIPXXXISFTXXIK*EEELNXII 539
ITVTIKATDHLRYLRICPDEIIYCSHSF IP ISFT +K*EEE N II
Sbjct: 205 ITVTIKATDHLRYLRICPDEIIYCSHSFKIPWRNISFTNFVK*EEECNHII 53
Score = 88.6 bits (187), Expect(5) = 2e-041
Identities = 37/52 (71%), Positives = 41/52 (78%)
Frame = +2 / -3
Query: 383 YHHRHYQSNGPS*ISQNLSR*NHLL*PFFXDPXXXYILYXXHQIRRRIEXXH 538
+HH HYQSNG S*ISQNLSR*NHLL*PFF DP YILY Q+RRR++ H
Sbjct: 209 FHHCHYQSNGSS*ISQNLSR*NHLL*PFFQDPMA*YILYQLRQMRRRMQPHH 54
Score = 87.7 bits (185), Expect(5) = 2e-047
Identities = 33/40 (82%), Positives = 34/40 (85%)
Frame = -1 / +2
Query: 500 GKGYXXXWDXERMATINDFIWTDSEISKMVRCFDSDGDDK 381
GKGY WD ERMATINDFIWTDSEISKM+RCFDSD D K
Sbjct: 92 GKGYITPWDLERMATINDFIWTDSEISKMIRCFDSDSDGK 211
Score = 85.4 bits (180), Expect(4) = 2e-027
Identities = 40/50 (80%), Positives = 41/50 (82%)
Frame = +1 / -2
Query: 388 SPSLSKQRTILDISESVQMKSFIVAILSXSHXXXYPLPXSSNKKKN*TXS 537
S SLSKQR ILDISESVQMKSFIVAILS SH YPLP SSN+KKN T S
Sbjct: 204 SLSLSKQRIILDISESVQMKSFIVAILSRSHGVIYPLPTSSNEKKNATTS 55
Score = 78.5 bits (165), Expect(4) = 3e-036
Identities = 38/51 (74%), Positives = 40/51 (78%)
Frame = -2 / +3
Query: 538 MXXFNSSSYLMXXVKDIXXXGIXKEWLQ*MISSGQILRYLRWSVALIVTVM 386
M +SSS+L VKDI GI KEWLQ*MISSGQILRYLR SVALIVTVM
Sbjct: 54 MMWLHSSSHLTKLVKDILRHGILKEWLQ*MISSGQILRYLR*SVALIVTVM 206
Score = 68.0 bits (142), Expect(4) = 3e-031
Identities = 37/46 (80%), Positives = 38/46 (82%)
Frame = -3 / +1
Query: 525 ILLLI**XX*RIXXXMGSXKNGYNK*FHLDRF*DI*DGPLL***R* 388
ILLLI* *RI MGS KNGYNK*FHLDRF*DI*D PLL***+*
Sbjct: 67 ILLLI*RSW*RIYYAMGS*KNGYNK*FHLDRF*DI*DDPLL***Q* 204
Score = 67.0 bits (140), Expect(5) = 1e-047
Identities = 24/25 (96%), Positives = 25/25 (100%)
Frame = +1 / -3
Query: 313 VILTSHAPATCCIEIQCCGNPRGLS 387
+ILTSHAPATCCIEIQCCGNPRGLS
Sbjct: 284 IILTSHAPATCCIEIQCCGNPRGLS 210
Score = 63.4 bits (132), Expect(5) = 2e-041
Identities = 24/24 (100%), Positives = 24/24 (100%)
Frame = +3 / -2
Query: 315 HPHFSCSCNMLH*DTMLRKSSRFI 386
HPHFSCSCNMLH*DTMLRKSSRFI
Sbjct: 282 HPHFSCSCNMLH*DTMLRKSSRFI 211
Score = 61.6 bits (128), Expect(5) = 2e-047
Identities = 24/24 (100%), Positives = 24/24 (100%)
Frame = -1 / +1
Query: 386 DKPRGFPQHCISMQHVAGA*EVRM 315
DKPRGFPQHCISMQHVAGA*EVRM
Sbjct: 211 DKPRGFPQHCISMQHVAGA*EVRM 282
Score = 57.4 bits (119), Expect(4) = 3e-036
Identities = 24/24 (100%), Positives = 24/24 (100%)
Frame = -3 / +3
Query: 384 *TSRISAALYLNATCCRSMRSEDD 313
*TSRISAALYLNATCCRSMRSEDD
Sbjct: 213 *TSRISAALYLNATCCRSMRSEDD 284
Score = 56.5 bits (117), Expect(4) = 3e-031
Identities = 24/24 (100%), Positives = 24/24 (100%)
Frame = -2 / +2
Query: 385 INLEDFRSIVSQCNMLQEHEK*G* 314
INLEDFRSIVSQCNMLQEHEK*G*
Sbjct: 212 INLEDFRSIVSQCNMLQEHEK*G* 283
Score = 54.2 bits (112), Expect(5) = 2e-047
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = -3 / +2
Query: 309 CCWSADACTLVLHGLTCEIASTLLG* 232
CCWS DACT+V+HG C+IASTLL *
Sbjct: 284 CCWSEDACTIVMHGPACDIASTLLA* 361
Score = 46.0 bits (94), Expect(4) = 3e-036
Identities = 19/26 (73%), Positives = 20/26 (76%)
Frame = -1 / +3
Query: 308 VAGLQMLVL*FCMA*PVKLHQHCWDS 231
VAGL+MLVL* CM PV LHQH W S
Sbjct: 285 VAGLKMLVL*LCMVQPVTLHQHYWHS 362
Score = 43.7 bits (89), Expect(5) = 1e-047
Identities = 18/25 (72%), Positives = 20/25 (80%)
Frame = +3 / -3
Query: 234 IPTVLMQFHRLGHAKLEYKHLQTSN 308
+P +LMQ HRL HA L YKHLQTSN
Sbjct: 359 MPVMLMQCHRLDHA*L*YKHLQTSN 285
Score = 43.2 bits (88), Expect(4) = 3e-031
Identities = 17/26 (65%), Positives = 21/26 (80%)
Frame = -2 / +1
Query: 310 VLLVCRCLYSSFAWPNL*NCINTVGI 233
+LLV RCLY S+AW +L*+CIN GI
Sbjct: 283 MLLV*RCLYYSYAWSSL*HCINITGI 360
Score = 39.6 bits (80), Expect(4) = 2e-027
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +2 / -1
Query: 338 QHVALRYNAAEILEVY 385
QHVALRYNAAEILEVY
Sbjct: 259 QHVALRYNAAEILEVY 212
Score = 38.6 bits (78), Expect(5) = 2e-041
Identities = 18/26 (69%), Positives = 18/26 (69%)
Frame = +1 / -1
Query: 232 LSQQC*CNFTG*AMQN*STSICRPAT 309
L Q C*CN TG M N STSI RPAT
Sbjct: 361 LCQ*C*CNVTGWTMHNYSTSIFRPAT 284
Score = 29.5 bits (58), Expect(5) = 2e-047
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = -1 / +1
Query: 182 SPVDRGFYDSTDLFGR 135
SP D+GFYDST LF R
Sbjct: 448 SPFDQGFYDST*LFLR 495
Score = 29.5 bits (58), Expect(5) = 2e-047
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / +1
Query: 217 PLNITFVVAACQ 182
PLNITFVV ACQ
Sbjct: 412 PLNITFVVGACQ 447
Score = 29.5 bits (58), Expect(5) = 1e-047
Identities = 11/13 (84%), Positives = 11/13 (84%)
Frame = +2 / -2
Query: 182 LTSCNYKGYVQWL 220
LTS N KGYVQWL
Sbjct: 447 LTSSNNKGYVQWL 409
Score = 28.1 bits (55), Expect(5) = 2e-041
Identities = 12/18 (66%), Positives = 12/18 (66%)
Frame = +1 / -1
Query: 166 PLSTGLDKLQLQRLCSMA 219
P DKLQ QRLCSMA
Sbjct: 463 PGQMDFDKLQQQRLCSMA 410
Score = 27.6 bits (54), Expect(4) = 2e-027
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +3 / -3
Query: 174 NWT*QAATTKVMFNG 218
N *QA TTKVMFNG
Sbjct: 455 NGL*QAPTTKVMFNG 411
Score = 26.3 bits (51), Expect(4) = 3e-036
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -1 / +3
Query: 218 AIEHNLCSCSLSSPV 174
AIEHNLC SLS +
Sbjct: 411 AIEHNLCCWSLSKSI 455
Score = 25.4 bits (49), Expect(4) = 2e-027
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +2 / -2
Query: 233 YPNSVDAISQVRPCKTRV 286
Y ++VDA+SQ PC T V
Sbjct: 360 YASNVDAMSQAGPCITIV 307
Score = 23.5 bits (45), Expect(4) = 3e-031
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = -3 / +2
Query: 219 SH*T*PL*LQLVK 181
SH*T*PL L+LVK
Sbjct: 410 SH*T*PLLLELVK 448
Score = 23.1 bits (44), Expect(5) = 1e-047
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +2 / -1
Query: 143 TSQ*NHKIPYQLD 181
T *NHKIP Q+D
Sbjct: 487 TVM*NHKIPGQMD 449
Score = 23.1 bits (44), Expect(5) = 2e-041
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +1 / -3
Query: 142 NKSVES*NPLSTGL 183
N VES*NP S GL
Sbjct: 488 NSHVES*NPWSNGL 447
>gb|BE358844.1|BE358844 DG1_32_D02.g1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 655
Score = 99.6 bits (211), Expect(4) = 5e-044
Identities = 42/51 (82%), Positives = 43/51 (84%)
Frame = +3 / -1
Query: 387 ITVTIKATDHLRYLRICPDEIIYCSHSFXIPXXXISFTXXIK*EEELNXII 539
ITVTIKATDHLRYLRICPDEIIYCSHSF IP ISFT +K*EEE N II
Sbjct: 325 ITVTIKATDHLRYLRICPDEIIYCSHSFKIPWRNISFTNFVK*EEECNHII 173
Score = 88.6 bits (187), Expect(4) = 4e-038
Identities = 37/52 (71%), Positives = 41/52 (78%)
Frame = +2 / -3
Query: 383 YHHRHYQSNGPS*ISQNLSR*NHLL*PFFXDPXXXYILYXXHQIRRRIEXXH 538
+HH HYQSNG S*ISQNLSR*NHLL*PFF DP YILY Q+RRR++ H
Sbjct: 329 FHHCHYQSNGSS*ISQNLSR*NHLL*PFFQDPMA*YILYQLRQMRRRMQPHH 174
Score = 87.7 bits (185), Expect(3) = 6e-040
Identities = 33/40 (82%), Positives = 34/40 (85%)
Frame = -1 / +2
Query: 500 GKGYXXXWDXERMATINDFIWTDSEISKMVRCFDSDGDDK 381
GKGY WD ERMATINDFIWTDSEISKM+RCFDSD D K
Sbjct: 212 GKGYITPWDLERMATINDFIWTDSEISKMIRCFDSDSDGK 331
Score = 85.4 bits (180), Expect(4) = 6e-026
Identities = 40/50 (80%), Positives = 41/50 (82%)
Frame = +1 / -2
Query: 388 SPSLSKQRTILDISESVQMKSFIVAILSXSHXXXYPLPXSSNKKKN*TXS 537
S SLSKQR ILDISESVQMKSFIVAILS SH YPLP SSN+KKN T S
Sbjct: 324 SLSLSKQRIILDISESVQMKSFIVAILSRSHGVIYPLPTSSNEKKNATTS 175
Score = 78.5 bits (165), Expect(3) = 1e-033
Identities = 38/51 (74%), Positives = 40/51 (78%)
Frame = -2 / +3
Query: 538 MXXFNSSSYLMXXVKDIXXXGIXKEWLQ*MISSGQILRYLRWSVALIVTVM 386
M +SSS+L VKDI GI KEWLQ*MISSGQILRYLR SVALIVTVM
Sbjct: 174 MMWLHSSSHLTKLVKDILRHGILKEWLQ*MISSGQILRYLR*SVALIVTVM 326
Score = 68.0 bits (142), Expect(3) = 2e-029
Identities = 37/46 (80%), Positives = 38/46 (82%)
Frame = -3 / +1
Query: 525 ILLLI**XX*RIXXXMGSXKNGYNK*FHLDRF*DI*DGPLL***R* 388
ILLLI* *RI MGS KNGYNK*FHLDRF*DI*D PLL***+*
Sbjct: 187 ILLLI*RSW*RIYYAMGS*KNGYNK*FHLDRF*DI*DDPLL***Q* 324
Score = 67.0 bits (140), Expect(4) = 5e-044
Identities = 24/25 (96%), Positives = 25/25 (100%)
Frame = +1 / -3
Query: 313 VILTSHAPATCCIEIQCCGNPRGLS 387
+ILTSHAPATCCIEIQCCGNPRGLS
Sbjct: 404 IILTSHAPATCCIEIQCCGNPRGLS 330
Score = 63.4 bits (132), Expect(4) = 4e-038
Identities = 24/24 (100%), Positives = 24/24 (100%)
Frame = +3 / -2
Query: 315 HPHFSCSCNMLH*DTMLRKSSRFI 386
HPHFSCSCNMLH*DTMLRKSSRFI
Sbjct: 402 HPHFSCSCNMLH*DTMLRKSSRFI 331
Score = 61.6 bits (128), Expect(3) = 6e-040
Identities = 24/24 (100%), Positives = 24/24 (100%)
Frame = -1 / +1
Query: 386 DKPRGFPQHCISMQHVAGA*EVRM 315
DKPRGFPQHCISMQHVAGA*EVRM
Sbjct: 331 DKPRGFPQHCISMQHVAGA*EVRM 402
Score = 57.4 bits (119), Expect(3) = 1e-033
Identities = 24/24 (100%), Positives = 24/24 (100%)
Frame = -3 / +3
Query: 384 *TSRISAALYLNATCCRSMRSEDD 313
*TSRISAALYLNATCCRSMRSEDD
Sbjct: 333 *TSRISAALYLNATCCRSMRSEDD 404
Score = 56.5 bits (117), Expect(3) = 2e-029
Identities = 24/24 (100%), Positives = 24/24 (100%)
Frame = -2 / +2
Query: 385 INLEDFRSIVSQCNMLQEHEK*G* 314
INLEDFRSIVSQCNMLQEHEK*G*
Sbjct: 332 INLEDFRSIVSQCNMLQEHEK*G* 403
Score = 54.2 bits (112), Expect(3) = 6e-040
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = -3 / +2
Query: 309 CCWSADACTLVLHGLTCEIASTLLG* 232
CCWS DACT+V+HG C+IASTLL *
Sbjct: 404 CCWSEDACTIVMHGPACDIASTLLA* 481
Score = 46.0 bits (94), Expect(3) = 1e-033
Identities = 19/26 (73%), Positives = 20/26 (76%)
Frame = -1 / +3
Query: 308 VAGLQMLVL*FCMA*PVKLHQHCWDS 231
VAGL+MLVL* CM PV LHQH W S
Sbjct: 405 VAGLKMLVL*LCMVQPVTLHQHYWHS 482
Score = 43.7 bits (89), Expect(4) = 5e-044
Identities = 18/25 (72%), Positives = 20/25 (80%)
Frame = +3 / -3
Query: 234 IPTVLMQFHRLGHAKLEYKHLQTSN 308
+P +LMQ HRL HA L YKHLQTSN
Sbjct: 479 MPVMLMQCHRLDHA*L*YKHLQTSN 405
Score = 43.2 bits (88), Expect(3) = 2e-029
Identities = 17/26 (65%), Positives = 21/26 (80%)
Frame = -2 / +1
Query: 310 VLLVCRCLYSSFAWPNL*NCINTVGI 233
+LLV RCLY S+AW +L*+CIN GI
Sbjct: 403 MLLV*RCLYYSYAWSSL*HCINITGI 480
Score = 39.6 bits (80), Expect(4) = 6e-026
Identities = 16/16 (100%), Positives = 16/16 (100%)
Frame = +2 / -1
Query: 338 QHVALRYNAAEILEVY 385
QHVALRYNAAEILEVY
Sbjct: 379 QHVALRYNAAEILEVY 332
Score = 38.6 bits (78), Expect(4) = 4e-038
Identities = 18/26 (69%), Positives = 18/26 (69%)
Frame = +1 / -1
Query: 232 LSQQC*CNFTG*AMQN*STSICRPAT 309
L Q C*CN TG M N STSI RPAT
Sbjct: 481 LCQ*C*CNVTGWTMHNYSTSIFRPAT 404
Score = 25.4 bits (49), Expect(4) = 6e-026
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +2 / -2
Query: 233 YPNSVDAISQVRPCKTRV 286
Y ++VDA+SQ PC T V
Sbjct: 480 YASNVDAMSQAGPCITIV 427
Score = 24.4 bits (47), Expect(4) = 5e-044
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +1 / -1
Query: 196 LQRLCSMALCPLLSQQC*CN 255
++ LC M LC L QQ C+
Sbjct: 595 IKSLCQMVLCTSLQQQRLCS 536
Score = 24.0 bits (46), Expect(4) = 4e-038
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +2 / -2
Query: 194 NYKGYVQWL 220
N KGYVQWL
Sbjct: 555 NNKGYVQWL 529
Score = 22.6 bits (43), Expect(4) = 6e-026
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +3 / -3
Query: 186 QAATTKVMFNG 218
++ TTKVMFNG
Sbjct: 563 KSPTTKVMFNG 531
>gb|CW176738.1|CW176738 104_589_11158647_148_36587_052 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11158647, DNA
sequence
Length = 628
Score = 67.0 bits (140), Expect(4) = 4e-023
Identities = 24/25 (96%), Positives = 25/25 (100%)
Frame = +1 / -1
Query: 313 VILTSHAPATCCIEIQCCGNPRGLS 387
+ILTSHAPATCCIEIQCCGNPRGLS
Sbjct: 214 IILTSHAPATCCIEIQCCGNPRGLS 140
Score = 61.6 bits (128), Expect(4) = 2e-026
Identities = 24/24 (100%), Positives = 24/24 (100%)
Frame = -1 / +3
Query: 386 DKPRGFPQHCISMQHVAGA*EVRM 315
DKPRGFPQHCISMQHVAGA*EVRM
Sbjct: 141 DKPRGFPQHCISMQHVAGA*EVRM 212
Score = 54.2 bits (112), Expect(4) = 2e-026
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = -3 / +1
Query: 309 CCWSADACTLVLHGLTCEIASTLLG* 232
CCWS DACT+V+HG C+IASTLL *
Sbjct: 214 CCWSEDACTIVMHGPACDIASTLLA* 291
Score = 43.7 bits (89), Expect(4) = 4e-023
Identities = 18/25 (72%), Positives = 20/25 (80%)
Frame = +3 / -1
Query: 234 IPTVLMQFHRLGHAKLEYKHLQTSN 308
+P +LMQ HRL HA L YKHLQTSN
Sbjct: 289 MPVMLMQCHRLDHA*L*YKHLQTSN 215
Score = 29.5 bits (58), Expect(4) = 2e-026
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = -1 / +3
Query: 182 SPVDRGFYDSTDLFGR 135
SP D+GFYDST LF R
Sbjct: 378 SPFDQGFYDST*LFLR 425
Score = 29.5 bits (58), Expect(4) = 2e-026
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / +3
Query: 217 PLNITFVVAACQ 182
PLNITFVV ACQ
Sbjct: 342 PLNITFVVGACQ 377
Score = 29.5 bits (58), Expect(4) = 4e-023
Identities = 11/13 (84%), Positives = 11/13 (84%)
Frame = +2 / -3
Query: 182 LTSCNYKGYVQWL 220
LTS N KGYVQWL
Sbjct: 377 LTSSNNKGYVQWL 339
Score = 23.1 bits (44), Expect(4) = 4e-023
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +2 / -2
Query: 143 TSQ*NHKIPYQLD 181
T *NHKIP Q+D
Sbjct: 417 TVM*NHKIPGQMD 379
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 574,044,064
Number of Sequences: 832831
Number of extensions: 7384028
Number of successful extensions: 77389
Number of sequences better than 1.0e-020: 3
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 15075460638
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)