TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3642920.2.1
(570 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW031244.1|CW031244 104_259_10500694_114_30397 Sorghum m... 95 4e-041
gb|BZ695088.1|BZ695088 SP__Ba0057N21.r SP__Ba Sorghum propi... 102 1e-036
gb|CW789437.1|CW789437 SP__Ba0057N21.r SP__Ba Sorghum propi... 102 1e-036
gb|CW388065.1|CW388065 fsbb001f072k16k0 Sorghum methylation... 104 1e-031
gb|CL179386.1|CL179386 104_388_10895272_148_31909_184 Sorgh... 89 3e-031
gb|BG357146.1|BG357146 OV2_10_D07.b1_A002 Ovary 2 (OV2) Sor... 95 4e-030
gb|CW374401.1|CW374401 fsbb001f051e01f0 Sorghum methylation... 131 1e-029
gb|CW423526.1|CW423526 fsbb001f133g04k0 Sorghum methylation... 67 2e-029
gb|CW471456.1|CW471456 fsbb001f225g01f0 Sorghum methylation... 115 7e-025
gb|CW229988.1|CW229988 104_671_11207609_148_37265_071 Sorgh... 82 1e-024
gb|CW392261.1|CW392261 fsbb001f078m20k0 Sorghum methylation... 111 6e-024
gb|CW158441.1|CW158441 104_564_11148823_148_36396_028 Sorgh... 82 1e-023
gb|BI139893.1|BI139893 IP1_47_A12.b1_A002 Immature pannicle... 107 2e-022
gb|CN140420.1|CN140420 OX1_36_B10.b1_A002 Oxidatively-stres... 105 4e-022
gb|CW068742.1|CW068742 104_317_10525284_1_30096 Sorghum met... 95 7e-022
>gb|CW031244.1|CW031244 104_259_10500694_114_30397 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10500694, DNA
sequence
Length = 776
Score = 94.5 bits (200), Expect(2) = 4e-041
Identities = 36/57 (63%), Positives = 47/57 (82%)
Frame = +3 / +3
Query: 345 SPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETC 515
+P Y ++LNH+R Y+PE++P L KVVFLDDD+VVQ DLS L+ I+L+G V GAVETC
Sbjct: 417 NPKYLSMLNHLRFYIPEIYPELQKVVFLDDDIVVQKDLSELFTINLNGNVMGAVETC 587
Score = 94.5 bits (200), Expect(2) = 4e-041
Identities = 38/92 (41%), Positives = 55/92 (59%)
Frame = +3 / +3
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVK 185
HF + SDNILA SVVV ST +S P+++VFH++TD Y M +WFA+N V+++
Sbjct: 111 HFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDDLNYAPMKAWFAMNDYRGVTVDIQ 290
Query: 186 GVHQFDWLTQENVPVLESIETQRAARDRYHGS 281
V F WL VPVL+ ++ + + GS
Sbjct: 291 KVEDFTWLNASYVPVLKQLQNAATQKFYFSGS 386
>gb|BZ695088.1|BZ695088 SP__Ba0057N21.r SP__Ba Sorghum propinquum genomic clone
SP__Ba0057N21 3', DNA sequence
Length = 1000
Score = 102 bits (217), Expect(3) = 1e-036
Identities = 36/63 (57%), Positives = 52/63 (82%)
Frame = +3 / -3
Query: 327 AKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAV 506
+ L+ +P Y ++LNH+R Y+PE++P L+K+VFLDDD+VV+ DL+ LW I++ GKVNGAV
Sbjct: 356 SNLKYRNPKYLSILNHLRFYLPEIYPKLDKMVFLDDDIVVKKDLTALWSINMKGKVNGAV 177
Query: 507 ETC 515
ETC
Sbjct: 176 ETC 168
Score = 55.6 bits (115), Expect(3) = 1e-036
Identities = 21/54 (38%), Positives = 29/54 (53%)
Frame = +3 / -3
Query: 81 PERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVKGVHQFDWLTQENVPVLESI 242
P VFH++TDK Y M WF N A +EV+ + +F WL PVL+ +
Sbjct: 572 PSDHVFHIVTDKLNYAPMRMWFLSNPPGKATIEVQHIGEFTWLNDSYSPVLKQL 411
Score = 34.1 bits (68), Expect(3) = 1e-036
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +3 / -2
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVI 107
H+ L SDNILA +VVV ST+ ++ + F VI
Sbjct: 732 HYALFSDNILATAVVVNSTVLNAKVRKFSTFMVI 631
>gb|CW789437.1|CW789437 SP__Ba0057N21.r SP__Ba Sorghum propinquum genomic clone
SP__Ba0057N21 3', DNA sequence
Length = 714
Score = 102 bits (217), Expect(3) = 1e-036
Identities = 36/63 (57%), Positives = 52/63 (82%)
Frame = +3 / -2
Query: 327 AKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAV 506
+ L+ +P Y ++LNH+R Y+PE++P L+K+VFLDDD+VV+ DL+ LW I++ GKVNGAV
Sbjct: 326 SNLKYRNPKYLSILNHLRFYLPEIYPKLDKMVFLDDDIVVKKDLTALWSINMKGKVNGAV 147
Query: 507 ETC 515
ETC
Sbjct: 146 ETC 138
Score = 55.6 bits (115), Expect(3) = 1e-036
Identities = 21/54 (38%), Positives = 29/54 (53%)
Frame = +3 / -2
Query: 81 PERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVKGVHQFDWLTQENVPVLESI 242
P VFH++TDK Y M WF N A +EV+ + +F WL PVL+ +
Sbjct: 542 PSDHVFHIVTDKLNYAPMRMWFLSNPPGKATIEVQHIGEFTWLNDSYSPVLKQL 381
Score = 34.1 bits (68), Expect(3) = 1e-036
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +3 / -1
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVI 107
H+ L SDNILA +VVV ST+ ++ + F VI
Sbjct: 702 HYALFSDNILATAVVVNSTVLNAKVRKFSTFMVI 601
>gb|CW388065.1|CW388065 fsbb001f072k16k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f072k16, DNA
sequence
Length = 722
Score = 104 bits (221), Expect(2) = 1e-031
Identities = 39/62 (62%), Positives = 49/62 (79%)
Frame = +3 / -2
Query: 348 PTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETCRGGD 527
P YT+ LNH+R Y+PE+FPSL KVV LD DVVVQ+DL+ LWD+D+ GKV GAVETC +
Sbjct: 304 PRYTSALNHLRFYLPEVFPSLGKVVLLDHDVVVQNDLTGLWDLDMKGKVIGAVETCTSSE 125
Query: 528 SW 533
+
Sbjct: 124 GY 119
Score = 55.1 bits (114), Expect(2) = 1e-031
Identities = 19/43 (44%), Positives = 26/43 (60%)
Frame = +3 / -2
Query: 81 PERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVKGVHQFDWL 209
P RI+ H++TD YPAM WF N +PA ++VK + WL
Sbjct: 478 PGRIILHIVTDALNYPAMMMWFLRNPPTPAAIQVKSLDDLKWL 350
>gb|CL179386.1|CL179386 104_388_10895272_148_31909_184 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10895272, DNA
sequence
Length = 735
Score = 89.0 bits (188), Expect(2) = 3e-031
Identities = 35/83 (42%), Positives = 52/83 (62%)
Frame = +3 / -3
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVK 185
H+ + SDN+LAASVVVRST+ ++ +PE+ VFH++TD+ + AM WF + A V V+
Sbjct: 436 HYAIFSDNVLAASVVVRSTVTNANEPEKHVFHIVTDRLNFAAMKMWFITHPPQLATVHVE 257
Query: 186 GVHQFDWLTQENVPVLESIETQR 254
+ F WL VL +E+ R
Sbjct: 256 NIDNFKWLNSSYCSVLRQLESAR 188
Score = 67.0 bits (140), Expect(2) = 3e-031
Identities = 25/42 (59%), Positives = 33/42 (78%)
Frame = +3 / -3
Query: 333 LQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDL 458
L+ +P Y ++LNH+R YMPE+ P L K++FLDDDVVVQ DL
Sbjct: 127 LKYRNPKYLSMLNHLRFYMPEIHPKLEKILFLDDDVVVQKDL 2
>gb|BG357146.1|BG357146 OV2_10_D07.b1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
sequence
Length = 538
Score = 94.5 bits (200), Expect(2) = 4e-030
Identities = 38/92 (41%), Positives = 55/92 (59%)
Frame = +3 / +1
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVK 185
HF + SDNILA SVVV ST +S P+++VFH++TD Y M +WFA+N V+++
Sbjct: 136 HFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDDLNYAPMKAWFAMNDYRGVTVDIQ 315
Query: 186 GVHQFDWLTQENVPVLESIETQRAARDRYHGS 281
V F WL VPVL+ ++ + + GS
Sbjct: 316 KVEDFTWLNASYVPVLKQLQNAATQKFYFSGS 411
Score = 57.9 bits (120), Expect(2) = 4e-030
Identities = 19/32 (59%), Positives = 27/32 (84%)
Frame = +3 / +1
Query: 345 SPTYTNVLNHIRIYMPELFPSLNKVVFLDDDV 440
+P Y ++LNH+R Y+PE++P L KVVFLDDD+
Sbjct: 442 NPKYLSMLNHLRFYIPEIYPELQKVVFLDDDI 537
>gb|CW374401.1|CW374401 fsbb001f051e01f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f051e01, DNA
sequence
Length = 432
Score = 131 bits (280), Expect = 1e-029
Identities = 49/59 (83%), Positives = 55/59 (93%)
Frame = +3 / +2
Query: 393 ELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETCRGGDSWVMSKRFRNYLNF 569
++FP+LNK FLDDDVVVQHDLSPLWDIDL+GK NGAVETCRGGDSWVMSK+F+NY NF
Sbjct: 68 QVFPNLNKAGFLDDDVVVQHDLSPLWDIDLAGKANGAVETCRGGDSWVMSKKFKNYFNF 244
Score = 116 bits (249), Expect = 2e-025
Identities = 50/57 (87%), Positives = 52/57 (91%)
Frame = +2 / +1
Query: 398 VPKPQQSRIS***CCCPA*PITTLGY*SIWEG*WRC*DLQRWR*LGDV*EVQELFKL 568
VPKPQQ RIS***CCCP *PITTLGY S WEG*W C*DLQRWR*LGDV*EVQ+LF+L
Sbjct: 73 VPKPQQGRIS***CCCPT*PITTLGYRSSWEG*WCC*DLQRWR*LGDV*EVQKLFQL 243
Score = 108 bits (230), Expect = 8e-023
Identities = 41/46 (89%), Positives = 43/46 (93%)
Frame = -1 / -2
Query: 534 PNYLHLCKSQQRH*PSQIDQYPKVVIGHAGQQHHHQEIRLC*GLGT 397
PNYLHLCKSQQ H*PSQ+D+YPKVVIGH GQQHHHQEIR C*GLGT
Sbjct: 209 PNYLHLCKSQQHH*PSQLDRYPKVVIGHVGQQHHHQEIRPC*GLGT 72
Score = 101 bits (216), Expect = 7e-021
Identities = 47/59 (79%), Positives = 49/59 (83%)
Frame = -2 / -3
Query: 569 KV*IIPEPLRHHPTISTSASLNSAINLPR*INIPKW**VMLDNNIIIKKYDFVEAWEQL 393
KV II E LRHHPTISTSASLNS I+LP *I+IPKW**VMLDNNIIIKK VE WE L
Sbjct: 244 KVEIIFELLRHHPTISTSASLNSTISLPS*IDIPKW**VMLDNNIIIKKSGLVEVWEHL 68
>gb|CW423526.1|CW423526 fsbb001f133g04k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f133g04, DNA
sequence
Length = 598
Score = 67.0 bits (140), Expect(3) = 2e-029
Identities = 22/45 (48%), Positives = 36/45 (80%)
Frame = +3 / -1
Query: 324 AAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDL 458
++ L+ +P Y ++LNH+R Y+P+++P NK++FLDDD+VVQ DL
Sbjct: 136 SSNLKYRNPKYLSMLNHLRFYLPQVYPKXNKILFLDDDIVVQRDL 2
Score = 63.8 bits (133), Expect(3) = 2e-029
Identities = 24/57 (42%), Positives = 34/57 (59%)
Frame = +3 / -1
Query: 78 KPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVKGVHQFDWLTQENVPVLESIET 248
+PE+ VFH++TDK + AM+ WF LN A + V+ V F WL PVL +E+
Sbjct: 361 EPEKHVFHLVTDKLNFGAMNMWFLLNPPGDATIHVENVDDFKWLNSSYCPVLRQLES 191
Score = 36.8 bits (74), Expect(3) = 2e-029
Identities = 15/23 (65%), Positives = 19/23 (82%)
Frame = +3 / -3
Query: 6 HFVLASDNILAASVVVRSTIRSS 74
H+ L SDN+LAASVVV STI ++
Sbjct: 539 HYALFSDNVLAASVVVNSTIMNA 471
>gb|CW471456.1|CW471456 fsbb001f225g01f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f225g01, DNA
sequence
Length = 473
Score = 115 bits (245), Expect = 7e-025
Identities = 43/67 (64%), Positives = 55/67 (82%)
Frame = +3 / +1
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVK 185
H L +DN+LAA+V V S +RS+ P R+VFHV+TDKK+Y MHSWFAL+ +SPA+VEVK
Sbjct: 31 HVALVTDNVLAAAVAVASAVRSADDPARLVFHVVTDKKSYVPMHSWFALHPVSPAVVEVK 210
Query: 186 GVHQFDW 206
G+HQFDW
Sbjct: 211 GLHQFDW 231
>gb|CW229988.1|CW229988 104_671_11207609_148_37265_071 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11207609, DNA
sequence
Length = 621
Score = 81.7 bits (172), Expect(2) = 1e-024
Identities = 29/54 (53%), Positives = 42/54 (77%)
Frame = +3 / -3
Query: 363 VLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETCRGG 524
+L+++R Y+PE+FP+L +VV L+DDVVVQ DL+ LW +D+ VN A+ TC GG
Sbjct: 373 LLDYLRFYLPEMFPALRRVVLLEDDVVVQRDLAGLWRVDMGANVNAALHTCFGG 212
Score = 53.3 bits (110), Expect(2) = 1e-024
Identities = 22/42 (52%), Positives = 27/42 (64%)
Frame = +3 / -3
Query: 24 DNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFA 149
DN+LA SVVV S R++ +P R VFHV+T PA WFA
Sbjct: 619 DNVLAVSVVVASAARAAAEPSRHVFHVVTAPMYLPAFRVWFA 494
>gb|CW392261.1|CW392261 fsbb001f078m20k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f078m20, DNA
sequence
Length = 343
Score = 111 bits (238), Expect = 6e-024
Identities = 42/64 (65%), Positives = 54/64 (84%)
Frame = +3 / -2
Query: 15 LASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVKGVH 194
L +DN+LAA+V V S +RS+ P R+VFHV+TDKK+Y MHSWFAL+ +SPA+VEVKG+H
Sbjct: 273 LVTDNVLAAAVAVTSAVRSADDPARLVFHVVTDKKSYVPMHSWFALHPVSPAVVEVKGLH 94
Query: 195 QFDW 206
QFDW
Sbjct: 93 QFDW 82
>gb|CW158441.1|CW158441 104_564_11148823_148_36396_028 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11148823, DNA
sequence
Length = 643
Score = 81.7 bits (172), Expect(2) = 1e-023
Identities = 29/54 (53%), Positives = 42/54 (77%)
Frame = +3 / -3
Query: 363 VLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETCRGG 524
+L+++R Y+PE+FP+L +VV L+DDVVVQ DL+ LW +D+ VN A+ TC GG
Sbjct: 398 LLDYLRFYLPEMFPALRRVVLLEDDVVVQRDLAGLWRVDMGANVNAALHTCFGG 237
Score = 50.6 bits (104), Expect(2) = 1e-023
Identities = 21/41 (51%), Positives = 26/41 (63%)
Frame = +3 / -3
Query: 27 NILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFA 149
N+LA SVVV S R++ +P R VFHV+T PA WFA
Sbjct: 641 NVLAVSVVVASAARAAAEPSRHVFHVVTAPMYLPAFRVWFA 519
>gb|BI139893.1|BI139893 IP1_47_A12.b1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA,
mRNA sequence
Length = 598
Score = 107 bits (228), Expect = 2e-022
Identities = 42/50 (84%), Positives = 44/50 (88%)
Frame = +3 / +2
Query: 420 VFLDDDVVVQHDLSPLWDIDLSGKVNGAVETCRGGDSWVMSKRFRNYLNF 569
VFLDDD+VVQ DLSPLW I+L GKVNGAVETCRG DSWVMSKRFR Y NF
Sbjct: 8 VFLDDDIVVQRDLSPLWAINLEGKVNGAVETCRGEDSWVMSKRFRTYFNF 157
>gb|CN140420.1|CN140420 OX1_36_B10.b1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_36_B10_A002 3', mRNA sequence
Length = 836
Score = 105 bits (225), Expect = 4e-022
Identities = 38/66 (57%), Positives = 54/66 (81%)
Frame = +3 / +3
Query: 324 AAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGA 503
A+ ++ +P Y ++LNH+R Y+PE++P L++++FLDDDVVVQ DL+ LW ID+ GKVNGA
Sbjct: 18 ASNMKFRNPKYLSMLNHLRFYLPEMYPKLHRILFLDDDVVVQRDLTGLWKIDMDGKVNGA 197
Query: 504 VETCRG 521
VETC G
Sbjct: 198 VETCFG 215
>gb|CW068742.1|CW068742 104_317_10525284_1_30096 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10525284, DNA
sequence
Length = 432
Score = 94.5 bits (200), Expect(2) = 7e-022
Identities = 36/57 (63%), Positives = 47/57 (82%)
Frame = +3 / -2
Query: 345 SPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETC 515
+P Y ++LNH+R Y+PE++P L KVVFLDDD+VVQ DLS L+ I+L+G V GAVETC
Sbjct: 284 NPKYLSMLNHLRFYIPEIYPELQKVVFLDDDIVVQKDLSELFTINLNGNVMGAVETC 114
Score = 29.9 bits (59), Expect(2) = 7e-022
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +3 / -2
Query: 174 VEVKGVHQFDWLTQENVPVLESIETQRAARDRYHGS 281
V+++ V F WL VPVL+ ++ + + GS
Sbjct: 422 VDIQKVEDFTWLNASYVPVLKQLQNAATQKFYFSGS 315
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 683,336,283
Number of Sequences: 832831
Number of extensions: 9421408
Number of successful extensions: 75544
Number of sequences better than 1.0e-020: 19
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 16271925768
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)