TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3202900.2.1
(938 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CW112315.1|CW112315 104_486_11104818_148_34542_077 Sorgh... 113 2e-069
>gb|CW112315.1|CW112315 104_486_11104818_148_34542_077 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11104818, DNA
sequence
Length = 632
Score = 113 bits (241), Expect(3) = 2e-069
Identities = 43/49 (87%), Positives = 45/49 (91%)
Frame = -1 / +3
Query: 749 EFGFLSMGHEFDSYPKGRIRAPKDWNWFLEEAKRTSDDEASGKRRSRSK 603
EFGFLSMGHEFDSYPKGRIRAPKDWNWFLEE KR+SDDEAS +R RSK
Sbjct: 111 EFGFLSMGHEFDSYPKGRIRAPKDWNWFLEETKRSSDDEASSSKRGRSK 257
Score = 101 bits (216), Expect(3) = 2e-067
Identities = 43/56 (76%), Positives = 46/56 (82%)
Frame = +3 / -1
Query: 582 LLVPSQNFRSRSPLAASFIVRGPLCFL*EPIPILWSTNPSLRVTVEFMAHRQEPKF 749
LL P+ FRS S AA FIVR PLCFL*+PIPILWSTNPSL V V+FMAHRQEPKF
Sbjct: 278 LLAPTLTFRSTSLAAAGFIVRAPLCFL*KPIPILWSTNPSLWVAVKFMAHRQEPKF 111
Score = 100 bits (212), Expect(3) = 2e-069
Identities = 45/68 (66%), Positives = 55/68 (80%)
Frame = -2 / +1
Query: 583 RRKMEKAKMKTGLVRVRMKKSPREELPV*RDQSIRQDLKAQKNIVGKTRRLEVAEVSTKM 404
RRK+EKAKMKTGLVRVRMK+SP +E V RDQS++QDLK QK++V KT+RLEVA+ KM
Sbjct: 274 RRKIEKAKMKTGLVRVRMKRSPWQEFLVLRDQSMQQDLKVQKSLVRKTQRLEVAKAWMKM 453
Query: 403 TALRMSKM 380
RM +M
Sbjct: 454 KGPRMKRM 477
Score = 100 bits (212), Expect(3) = 2e-067
Identities = 47/69 (68%), Positives = 51/69 (73%)
Frame = +3 / -1
Query: 378 LILLILSAVIFVDTSATSSLRVFPTMFF*AFRSCRILWSLHTGNSSRGLFFILTLTSPVF 557
LIL IL IF+ ATS+L VF T F FRSC ILWSL+T NS +GL FILTLTSPVF
Sbjct: 479 LILFILGPFIFIHAFATSNL*VFLTRLFWTFRSCCILWSLNTRNSCQGLLFILTLTSPVF 300
Query: 558 IFAFSIFLL 584
I AFSIFLL
Sbjct: 299 ILAFSIFLL 273
Score = 97.3 bits (206), Expect(3) = 8e-040
Identities = 42/58 (72%), Positives = 47/58 (81%)
Frame = -2 / +1
Query: 751 ENLGSCRWAMNSTVTRREGFVLQRIGIGS*RKQRGPLTMKLAARGDLDRKFWDGTRRR 578
+NLGSCRWAMN T T+REGFVLQRIGIG *RKQRG LTMK AA ++DRK G RR+
Sbjct: 109 QNLGSCRWAMNLTATQREGFVLQRIGIGF*RKQRGALTMKPAAAREVDRKVRVGARRK 282
Score = 95.0 bits (201), Expect(3) = 2e-067
Identities = 38/47 (80%), Positives = 39/47 (82%)
Frame = +2 / -3
Query: 254 RRFFFDVVINHHLPHRQIPPLHRSALLQARHPRR*LKNLPESHLAHP 394
RR F VVINH LPH QIPPLHRSALL+ARHPR *LKNLPE H AHP
Sbjct: 612 RRLIFGVVINHRLPHHQIPPLHRSALLEARHPRH*LKNLPEFHPAHP 472
Score = 91.3 bits (193), Expect(3) = 8e-037
Identities = 38/52 (73%), Positives = 43/52 (82%)
Frame = +2 / -3
Query: 599 ELSI*ISSCR*LHRQRSSLLPLGTNSNPLEHESFPSGNCRIHGPSTGTQILE 754
+LSI +S C LHRQ SSL PL TNSNPLEHESFP G+C+IHGPST TQIL+
Sbjct: 261 DLSIYLSCCCWLHRQSSSLFPLETNSNPLEHESFPLGSCQIHGPSTRTQILQ 106
Score = 90.4 bits (191), Expect(2) = 2e-030
Identities = 37/40 (92%), Positives = 39/40 (97%)
Frame = +1 / -2
Query: 631 ASSSEVLFASSRNQFQSFGARILPFG*LSNSWPIDRNPNS 750
ASSSE+LF SSRNQFQSFGARILPFG*LSNSWPID+NPNS
Sbjct: 229 ASSSELLFVSSRNQFQSFGARILPFG*LSNSWPIDKNPNS 110
Score = 90.0 bits (190), Expect(3) = 2e-069
Identities = 42/53 (79%), Positives = 44/53 (83%)
Frame = -2 / +1
Query: 394 RMSKMRLWEVF*LTTRMTSLQKS*AMKRRNLTMRKMMIDNHVKKEPSQ**TDT 236
RMS+M+LWEVF*L TRMTSL KS* MKRRNL MRK MIDNH K SQ**TDT
Sbjct: 472 RMSRMKLWEVF*LMTRMTSL*KS*TMKRRNLMMRKTMIDNHAKN*SSQ**TDT 630
Score = 75.8 bits (159), Expect(3) = 1e-027
Identities = 34/42 (80%), Positives = 38/42 (90%)
Frame = -3 / +2
Query: 747 IWVPVDGP*IRQLPEGKDSCSKGLELVPRGSKEDL*R*S*RQ 622
IWV VDGP*I QLP+GKDSCSKGLELV RG+KE+L*R*S +Q
Sbjct: 113 IWVLVDGP*I*QLPKGKDSCSKGLELVSRGNKEEL*R*SQQQ 238
Score = 72.1 bits (151), Expect(3) = 8e-040
Identities = 37/55 (67%), Positives = 42/55 (76%)
Frame = -3 / +2
Query: 552 LDW*E*G*KRVHGKSYQCEETKVYDKI*KLRKT**GKLEDWKWQKYRRR*RH*G* 388
LDW*E*G*K V GKS C ETKV +KI*+ +K **GKL+ WKWQK+ R*R G*
Sbjct: 305 LDW*E*G*KGVPGKSS*C*ETKVCNKI*RSKKA**GKLKGWKWQKHG*R*RGRG* 469
Score = 66.1 bits (138), Expect(2) = 2e-030
Identities = 34/54 (62%), Positives = 35/54 (64%)
Frame = +3 / -1
Query: 234 SVSVHH*EGSFLTWLSXXXXXXXXXXXXXAQLFCRLVILVVN*KTSQSLILLIL 395
SVSVHH*E FL WLS QLF RLVILV+N*KTSQS ILLIL
Sbjct: 632 SVSVHH*ED*FLAWLSIIVFLIIKFLLFIVQLF*RLVILVIN*KTSQSFILLIL 471
Score = 62.0 bits (129), Expect(3) = 1e-027
Identities = 26/38 (68%), Positives = 30/38 (78%)
Frame = -1 / +3
Query: 527 KESTGRVTSVKRPKYTTRSKSSEKHSRENSKTGSGRSI 414
KES RV SVKRPKY TRSK +K S+ENSK GSG+S+
Sbjct: 330 KESLARVPSVKRPKYATRSKGPKKPSKENSKVGSGKSM 443
Score = 62.0 bits (129), Expect(3) = 8e-037
Identities = 31/63 (49%), Positives = 35/63 (55%)
Frame = +2 / -3
Query: 341 RHPRR*LKNLPESHLAHPQCRHLRRYFCHFQSSSFPYYVFLSF*ILSYTLVSSHW*LFPW 520
RH + L +H HP+ HL FCHFQ SFPY FL *IL +TLVS H L P
Sbjct: 516 RH*LKNLPEFHPAHPLHPRPLHLHPCFCHFQPLSFPY*AFLDL*ILLHTLVS*H*ELLPG 337
Query: 521 TLF 529
T F
Sbjct: 336 TPF 328
Score = 40.5 bits (82), Expect(3) = 8e-037
Identities = 17/44 (38%), Positives = 18/44 (40%)
Frame = +1 / -2
Query: 235 QYQFITEKVLF*RGYQXXXXXXXXXXXXXXXXXXXXXXXXLTKK 366
QYQFITEK+ F RGYQ LTKK
Sbjct: 631 QYQFITEKINFWRGYQSSSSSSSNSSSSSFSSSRGSSSSSLTKK 500
Score = 34.5 bits (69), Expect(3) = 8e-040
Identities = 18/21 (85%), Positives = 18/21 (85%)
Frame = -3 / +2
Query: 297 *GR**LITTSKKNLLSDELIL 235
*GR *LITT K NLLSDELIL
Sbjct: 569 *GRR*LITTPKINLLSDELIL 631
Score = 28.1 bits (55), Expect(3) = 1e-027
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = -1 / +3
Query: 281 **PRQKRTFSVMN*Y* 234
**PRQK FSVMN*Y*
Sbjct: 585 **PRQKLIFSVMN*Y* 632
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 868,916,454
Number of Sequences: 832831
Number of extensions: 11257982
Number of successful extensions: 68984
Number of sequences better than 1.0e-020: 1
length of database: 163,786,556
effective HSP length: 55
effective length of database: 117,980,851
effective search space used: 30321078707
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)