TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3089980.2.1
(540 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX611172.1|CX611172 ANR1_23_C03.b1_A002 Anaerobic roots ... 123 3e-033
gb|BG356912.1|BG356912 OV2_11_E10.g1_A002 Ovary 2 (OV2) Sor... 123 3e-033
gb|CX608344.1|CX608344 ANR1_38_C03.b1_A002 Anaerobic roots ... 119 8e-032
gb|BG054078.1|BG054078 OV2_3_B08.g1_A002 Ovary 2 (OV2) Sorg... 116 5e-031
gb|CW241116.1|CW241116 104_700_11218590_148_37543_029 Sorgh... 99 1e-028
gb|BF317701.1|BF317701 OV1_12_C03.g1_A002 Ovary 1 (OV1) Sor... 98 2e-025
gb|BG356656.1|BG356656 OV2_26_B11.g1_A002 Ovary 2 (OV2) Sor... 96 7e-025
gb|CN137974.1|CN137974 OX1_61_A09.b1_A002 Oxidatively-stres... 96 3e-024
>gb|CX611172.1|CX611172 ANR1_23_C03.b1_A002 Anaerobic roots Sorghum bicolor cDNA clone
ANR1_23_C03_A002 3', mRNA sequence
Length = 701
Score = 123 bits (264), Expect(2) = 3e-033
Identities = 49/99 (49%), Positives = 68/99 (68%)
Frame = -2 / +3
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
+A V GD T+ +FV E D+ TV A DDPRA +KTLY++PPANT S L+ LWE KT
Sbjct: 81 KAVVLGDGDTKVVFVEEGDIGTYTVLAADDPRAENKTLYIKPPANTLSHNELLSLWEKKT 260
Query: 359 GRALDKYYMPDEELVNRIRDSPLPLNFQLAMVHATVVAG 243
G+ + Y+P+E ++ +I++SP+PLN LA+ HA V G
Sbjct: 261 GKTFQREYVPEEAVLKQIQESPIPLNIILAIGHAAYVRG 377
Score = 38.6 bits (78), Expect(2) = 3e-033
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = -2 / +3
Query: 206 VEATELYPDVNYVTVHDYLDGLKESLTR 123
V+ATELYPDV Y TV +YL+ SL +
Sbjct: 414 VDATELYPDVKYTTVDEYLNRFL*SLKK 497
>gb|BG356912.1|BG356912 OV2_11_E10.g1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
sequence
Length = 647
Score = 123 bits (264), Expect(2) = 3e-033
Identities = 49/99 (49%), Positives = 68/99 (68%)
Frame = -2 / +2
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
+A V GD T+ +FV E D+ TV A DDPRA +KTLY++PPANT S L+ LWE KT
Sbjct: 59 KAVVLGDGDTKVVFVEEGDIGTYTVLAADDPRAENKTLYIKPPANTLSHNELLSLWEKKT 238
Query: 359 GRALDKYYMPDEELVNRIRDSPLPLNFQLAMVHATVVAG 243
G+ + Y+P+E ++ +I++SP+PLN LA+ HA V G
Sbjct: 239 GKTFQREYVPEEAVLKQIQESPIPLNIILAIGHAAYVRG 355
Score = 38.6 bits (78), Expect(2) = 3e-033
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = -2 / +2
Query: 206 VEATELYPDVNYVTVHDYLDGLKESLTR 123
V+ATELYPDV Y TV +YL+ SL +
Sbjct: 392 VDATELYPDVKYTTVDEYLNRFL*SLKK 475
>gb|CX608344.1|CX608344 ANR1_38_C03.b1_A002 Anaerobic roots Sorghum bicolor cDNA clone
ANR1_38_C03_A002 3', mRNA sequence
Length = 605
Score = 119 bits (254), Expect(2) = 8e-032
Identities = 47/94 (50%), Positives = 65/94 (69%)
Frame = -2 / +2
Query: 524 GDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKTGRALD 345
GD T+ +FV E D+ TV A DDPRA +KTLY++PPANT S L+ LWE KTG+
Sbjct: 2 GDGDTKVVFVEEGDIGTYTVLAADDPRAENKTLYIKPPANTLSHNELLSLWEKKTGKTFQ 181
Query: 344 KYYMPDEELVNRIRDSPLPLNFQLAMVHATVVAG 243
+ Y+P+E ++ +I++SP+PLN LA+ HA V G
Sbjct: 182 REYVPEEAVLKQIQESPIPLNIILAIGHAAYVRG 283
Score = 38.6 bits (78), Expect(2) = 8e-032
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = -2 / +2
Query: 206 VEATELYPDVNYVTVHDYLDGLKESLTR 123
V+ATELYPDV Y TV +YL+ SL +
Sbjct: 320 VDATELYPDVKYTTVDEYLNRFL*SLKK 403
>gb|BG054078.1|BG054078 OV2_3_B08.g1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA sequence
Length = 494
Score = 116 bits (248), Expect(2) = 5e-031
Identities = 46/93 (49%), Positives = 64/93 (68%)
Frame = -2 / +2
Query: 521 DDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKTGRALDK 342
D T+ +FV E D+ TV A DDPRA +KTLY++PPANT S L+ LWE KTG+ +
Sbjct: 2 DGDTKVVFVEEGDIGTYTVLAADDPRAENKTLYIKPPANTLSHNELLSLWEKKTGKTFQR 181
Query: 341 YYMPDEELVNRIRDSPLPLNFQLAMVHATVVAG 243
Y+P+E ++ +I++SP+PLN LA+ HA V G
Sbjct: 182 EYVPEEAVLKQIQESPIPLNIILAIGHAAYVRG 280
Score = 38.6 bits (78), Expect(2) = 5e-031
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = -2 / +2
Query: 206 VEATELYPDVNYVTVHDYLDGLKESLTR 123
V+ATELYPDV Y TV +YL+ SL +
Sbjct: 317 VDATELYPDVKYTTVDEYLNRFL*SLKK 400
>gb|CW241116.1|CW241116 104_700_11218590_148_37543_029 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11218590, DNA
sequence
Length = 600
Score = 98.7 bits (209), Expect(3) = 1e-028
Identities = 38/79 (48%), Positives = 54/79 (68%)
Frame = -2 / +2
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
+A V GD T+ +FV E D+ TV A DDPRA +KTLY++PPANT S L+ LWE KT
Sbjct: 92 KAVVLGDGDTKVVFVEEGDIGTYTVLAADDPRAENKTLYIKPPANTLSHNELLSLWEKKT 271
Query: 359 GRALDKYYMPDEELVNRIR 303
G+ + Y+P+E ++ +I+
Sbjct: 272 GKTFQREYVPEEAVLKQIQ 328
Score = 38.2 bits (77), Expect(3) = 1e-028
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = -2 / +3
Query: 206 VEATELYPDVNYVTVHDYLD 147
V+ATELYPDV Y TV +YL+
Sbjct: 528 VDATELYPDVKYTTVDEYLN 587
Score = 28.1 bits (55), Expect(3) = 1e-028
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -2 / +3
Query: 302 DSPLPLNFQLAMVHATVVAG 243
+SP+PLN LA+ HA V G
Sbjct: 432 ESPIPLNIILAIGHAAYVRG 491
>gb|BF317701.1|BF317701 OV1_12_C03.g1_A002 Ovary 1 (OV1) Sorghum bicolor cDNA, mRNA
sequence
Length = 533
Score = 97.7 bits (207), Expect(2) = 2e-025
Identities = 38/73 (52%), Positives = 53/73 (72%)
Frame = -2 / +1
Query: 461 AVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKTGRALDKYYMPDEELVNRIRDSPLPLN 282
A DDPRA +KTLY++PPANT S L+ LWE KTG+ + Y+P+E ++ +I++SP+PLN
Sbjct: 1 AADDPRAENKTLYIKPPANTLSHNELLSLWEKKTGKTFQREYVPEEAVLKQIQESPIPLN 180
Query: 281 FQLAMVHATVVAG 243
LA+ HA V G
Sbjct: 181 IILAIGHAAYVRG 219
Score = 38.6 bits (78), Expect(2) = 2e-025
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = -2 / +1
Query: 206 VEATELYPDVNYVTVHDYLDGLKESLTR 123
V+ATELYPDV Y TV +YL+ SL +
Sbjct: 256 VDATELYPDVKYTTVDEYLNRFL*SLKK 339
>gb|BG356656.1|BG356656 OV2_26_B11.g1_A002 Ovary 2 (OV2) Sorghum bicolor cDNA, mRNA
sequence
Length = 524
Score = 95.9 bits (203), Expect(2) = 7e-025
Identities = 37/71 (52%), Positives = 52/71 (73%)
Frame = -2 / +2
Query: 455 DDPRALDKTLYLRPPANTCSLAHLVRLWEDKTGRALDKYYMPDEELVNRIRDSPLPLNFQ 276
DDPRA +KTLY++PPANT S L+ LWE KTG+ + Y+P+E ++ +I++SP+PLN
Sbjct: 5 DDPRAENKTLYIKPPANTLSHNELLSLWEKKTGKTFQREYVPEEAVLKQIQESPIPLNII 184
Query: 275 LAMVHATVVAG 243
LA+ HA V G
Sbjct: 185 LAIGHAAYVRG 217
Score = 38.6 bits (78), Expect(2) = 7e-025
Identities = 16/28 (57%), Positives = 20/28 (71%)
Frame = -2 / +2
Query: 206 VEATELYPDVNYVTVHDYLDGLKESLTR 123
V+ATELYPDV Y TV +YL+ SL +
Sbjct: 254 VDATELYPDVKYTTVDEYLNRFL*SLKK 337
>gb|CN137974.1|CN137974 OX1_61_A09.b1_A002 Oxidatively-stressed leaves and roots Sorghum
bicolor cDNA clone OX1_61_A09_A002 3', mRNA sequence
Length = 703
Score = 96.4 bits (204), Expect(2) = 3e-024
Identities = 37/77 (48%), Positives = 52/77 (67%)
Frame = -2 / +1
Query: 539 RATVFGDDATRAIFVHEADMAAVTVRAVDDPRALDKTLYLRPPANTCSLAHLVRLWEDKT 360
+A V GD T+ +FV E D+ TV A DDPRA +KTLY++PPANT S L+ LWE KT
Sbjct: 154 KAVVLGDGDTKVVFVEEGDIGTYTVLAADDPRAENKTLYIKPPANTLSHNELLSLWEKKT 333
Query: 359 GRALDKYYMPDEELVNR 309
G+ + Y+P+E ++ +
Sbjct: 334 GKTFQREYVPEEAVLKQ 384
Score = 35.9 bits (72), Expect(2) = 3e-024
Identities = 15/28 (53%), Positives = 19/28 (67%)
Frame = -2 / +1
Query: 206 VEATELYPDVNYVTVHDYLDGLKESLTR 123
V+ATELYPDV Y V +YL+ SL +
Sbjct: 415 VDATELYPDVKYTPVDEYLNRFL*SLKK 498
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 382,134,326
Number of Sequences: 832831
Number of extensions: 5163412
Number of successful extensions: 180828
Number of sequences better than 1.0e-020: 8
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 15075460638
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)