TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2823202.2.1
(529 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX608155.1|CX608155 ANR1_37_B02.b1_A002 Anaerobic roots ... 165 2e-042
gb|BG559678.1|BG559678 RHIZ2_72_E04.g1_A003 Rhizome2 (RHIZ2... 165 5e-041
gb|BM328522.1|BM328522 PIC1_30_C03.g1_A002 Pathogen-infecte... 134 1e-034
gb|CW376050.1|CW376050 fsbb001f053l16k0 Sorghum methylation... 79 3e-027
>gb|CX608155.1|CX608155 ANR1_37_B02.b1_A002 Anaerobic roots Sorghum bicolor cDNA clone
ANR1_37_B02_A002 3', mRNA sequence
Length = 633
Score = 165 bits (354), Expect(3) = 2e-042
Identities = 71/89 (79%), Positives = 76/89 (85%)
Frame = +3 / +3
Query: 6 RAIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFA 185
+AIQQNIT CRSRTKTCA+KYAAESAKLACTAYEGV+Q+STLEDDYFFA
Sbjct: 141 QAIQQNITEEWANEEKQWETCRSRTKTCAEKYAAESAKLACTAYEGVEQESTLEDDYFFA 320
Query: 186 ALPVVQKRIAQGGVRLAAILNRIFGGNSR 272
ALPVVQKRIAQGGVRLAAILN+IFGG SR
Sbjct: 321 ALPVVQKRIAQGGVRLAAILNKIFGGKSR 407
Score = 116 bits (247), Expect = 3e-025
Identities = 50/68 (73%), Positives = 53/68 (77%)
Frame = +1 / +1
Query: 67 AVAGQRPVLTSTLQRARSWPARRTRVSTKTPPWKMTTSSPRCRWFRRGSRKGASGWPRSS 246
AVAGQR L STLQRARSW ARRTRVS++ PP KMTTSS CRWFRRG KG SGWP+
Sbjct: 202 AVAGQRLALKSTLQRARSWHARRTRVSSRNPP*KMTTSSLHCRWFRRGLLKGVSGWPQY* 381
Query: 247 TGYLVGTA 270
T YLVG A
Sbjct: 382 TRYLVGRA 405
Score = 98.7 bits (209), Expect(2) = 1e-022
Identities = 49/90 (54%), Positives = 51/90 (56%)
Frame = -1 / -3
Query: 271 LLFPPNILLRIAASLTPPCAILF*TTGXXXXXXXXXKVESWSTPSYAVQXXXXXXXXXXX 92
LLFPPNIL IAAS TPP AILF*TTG KV+S STPSYAV
Sbjct: 406 LLFPPNILFSIAASRTPP*AILF*TTGSAAKK*SSSKVDSCSTPSYAVHASFALSAAYFS 227
Query: 91 AQVFVRLRQXXXXXXXXXXXSVMFCWMALV 2
AQVFVRLR SVMFCWMA +
Sbjct: 226 AQVFVRLRHVSHCFSSLAHSSVMFCWMACI 137
Score = 97.7 bits (207), Expect(4) = 6e-025
Identities = 45/60 (75%), Positives = 48/60 (80%)
Frame = -3 / -2
Query: 269 AVPTKYPVEDRGQPDAPLRDPLLNHRQRGEEVVIFQGGVLVDTLVRRAGQLRALCSVLVS 90
A+PTKY V GQPD PL +PLLNHRQ EEVVIF GG L+DTLVRRA QLRALCSVL S
Sbjct: 404 ALPTKYLV*YCGQPDTPLSNPLLNHRQCSEEVVIF*GGFLLDTLVRRACQLRALCSVLFS 225
Score = 28.6 bits (56), Expect(4) = 6e-025
Identities = 10/14 (71%), Positives = 13/14 (92%)
Frame = -2 / -1
Query: 396 IILIQMADHACPSP 355
+ILIQ+ADHA P+P
Sbjct: 504 LILIQIADHAIPTP 463
Score = 28.1 bits (55), Expect(2) = 1e-022
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = -3 / -2
Query: 416 TATRYLLLF*FRWQIMPAPPHPLLHMSNRLISTTGMDM 303
T + +LLF*FR Q MP+P L + + +T +++
Sbjct: 524 TLSDKILLF*FRLQTMPSPLRSCLTSTGWMDQSTALNL 411
Score = 23.5 bits (45), Expect(3) = 2e-042
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +1 / +1
Query: 361 GAGMICHLN*N 393
G GM+C+LN*N
Sbjct: 469 GDGMVCNLN*N 501
Score = 23.1 bits (44), Expect(3) = 2e-042
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +3 / +1
Query: 249 RIFGGNSRSGFRAVD*SIH 305
R G + FRAVD*SIH
Sbjct: 385 RYLVGRAGFRFRAVD*SIH 441
Score = 22.1 bits (42), Expect(4) = 6e-025
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -3 / -1
Query: 305 MDRSINCSEA 276
MD SINCSE+
Sbjct: 441 MDGSINCSES 412
Score = 21.7 bits (41), Expect(4) = 6e-025
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = -2 / -1
Query: 24 CSAGWP 7
CSAGWP
Sbjct: 159 CSAGWP 142
>gb|BG559678.1|BG559678 RHIZ2_72_E04.g1_A003 Rhizome2 (RHIZ2) Sorghum propinquum cDNA, mRNA
sequence
Length = 558
Score = 165 bits (354), Expect(2) = 5e-041
Identities = 71/89 (79%), Positives = 76/89 (85%)
Frame = +3 / +2
Query: 6 RAIQQNITXXXXXXXXXXXXCRSRTKTCADKYAAESAKLACTAYEGVDQDSTLEDDYFFA 185
+AIQQNIT CRSRTKTCA+KYAAESAKLACTAYEGV+Q+STLEDDYFFA
Sbjct: 119 QAIQQNITEEWANEEKQWETCRSRTKTCAEKYAAESAKLACTAYEGVEQESTLEDDYFFA 298
Query: 186 ALPVVQKRIAQGGVRLAAILNRIFGGNSR 272
ALPVVQKRIAQGGVRLAAILN+IFGG SR
Sbjct: 299 ALPVVQKRIAQGGVRLAAILNKIFGGKSR 385
Score = 118 bits (252), Expect = 7e-026
Identities = 51/68 (75%), Positives = 54/68 (79%)
Frame = +1 / +3
Query: 67 AVAGQRPVLTSTLQRARSWPARRTRVSTKTPPWKMTTSSPRCRWFRRGSRKGASGWPRSS 246
AVAGQR L STLQRARSW ARRTRVS++ PP KMTTSS RCRWFRRG KG SGWP+
Sbjct: 180 AVAGQRLALKSTLQRARSWHARRTRVSSRNPP*KMTTSSLRCRWFRRGLLKGVSGWPQY* 359
Query: 247 TGYLVGTA 270
T YLVG A
Sbjct: 360 TRYLVGRA 383
Score = 101 bits (215), Expect(3) = 2e-024
Identities = 46/60 (76%), Positives = 49/60 (81%)
Frame = -3 / -3
Query: 269 AVPTKYPVEDRGQPDAPLRDPLLNHRQRGEEVVIFQGGVLVDTLVRRAGQLRALCSVLVS 90
A+PTKY V GQPD PL +PLLNHRQR EEVVIF GG L+DTLVRRA QLRALCSVL S
Sbjct: 382 ALPTKYLV*YCGQPDTPLSNPLLNHRQRSEEVVIF*GGFLLDTLVRRACQLRALCSVLFS 203
Score = 98.7 bits (209), Expect(2) = 3e-022
Identities = 49/90 (54%), Positives = 51/90 (56%)
Frame = -1 / -1
Query: 271 LLFPPNILLRIAASLTPPCAILF*TTGXXXXXXXXXKVESWSTPSYAVQXXXXXXXXXXX 92
LLFPPNIL IAAS TPP AILF*TTG KV+S STPSYAV
Sbjct: 384 LLFPPNILFSIAASRTPP*AILF*TTGSAAKK*SSSKVDSCSTPSYAVHASFALSAAYFS 205
Query: 91 AQVFVRLRQXXXXXXXXXXXSVMFCWMALV 2
AQVFVRLR SVMFCWMA +
Sbjct: 204 AQVFVRLRHVSHCFSSLAHSSVMFCWMACI 115
Score = 28.6 bits (56), Expect(3) = 2e-024
Identities = 10/14 (71%), Positives = 13/14 (92%)
Frame = -2 / -2
Query: 396 IILIQMADHACPSP 355
+ILIQ+ADHA P+P
Sbjct: 482 LILIQIADHAIPTP 441
Score = 27.2 bits (53), Expect(2) = 3e-022
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = -3 / -3
Query: 416 TATRYLLLF*FRWQIMPAPPHPLLHMSNRLISTTGMDM 303
T + +LLF*FR Q MP+P L + + +T +++
Sbjct: 502 TLSDKILLF*FRLQTMPSPLLSCLTSTGLMDQSTALNL 389
Score = 23.5 bits (45), Expect(2) = 5e-041
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +1 / +3
Query: 361 GAGMICHLN*N 393
G GM+C+LN*N
Sbjct: 447 GDGMVCNLN*N 479
Score = 21.7 bits (41), Expect(3) = 2e-024
Identities = 6/6 (100%), Positives = 6/6 (100%)
Frame = -2 / -2
Query: 24 CSAGWP 7
CSAGWP
Sbjct: 137 CSAGWP 120
>gb|BM328522.1|BM328522 PIC1_30_C03.g1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum
bicolor cDNA, mRNA sequence
Length = 419
Score = 134 bits (288), Expect(4) = 1e-034
Identities = 56/62 (90%), Positives = 60/62 (96%)
Frame = +3 / +3
Query: 87 CADKYAAESAKLACTAYEGVDQDSTLEDDYFFAALPVVQKRIAQGGVRLAAILNRIFGGN 266
CA+KYAAESAKLACTAYEGV+Q+S LEDDYFFAALPVVQKRIAQGGVRLAAILN+IFGG
Sbjct: 3 CAEKYAAESAKLACTAYEGVEQESPLEDDYFFAALPVVQKRIAQGGVRLAAILNKIFGGK 182
Query: 267 SR 272
SR
Sbjct: 183 SR 188
Score = 95.5 bits (202), Expect(3) = 9e-023
Identities = 43/60 (71%), Positives = 48/60 (80%)
Frame = -3 / -1
Query: 269 AVPTKYPVEDRGQPDAPLRDPLLNHRQRGEEVVIFQGGVLVDTLVRRAGQLRALCSVLVS 90
A+PTKY V GQPD PL +PLLNHRQ E+V+IF GG L+DTLVRRA QLRALCSVL S
Sbjct: 185 ALPTKYLV*YCGQPDTPLSNPLLNHRQCSEKVIIF*GGFLLDTLVRRACQLRALCSVLFS 6
Score = 26.3 bits (51), Expect(3) = 9e-023
Identities = 9/14 (64%), Positives = 13/14 (92%)
Frame = -2 / -3
Query: 396 IILIQMADHACPSP 355
+ILIQ+A+HA P+P
Sbjct: 285 LILIQIANHAIPTP 244
Score = 23.5 bits (45), Expect(4) = 1e-034
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +1 / +1
Query: 361 GAGMICHLN*N 393
G GM+C+LN*N
Sbjct: 250 GDGMVCNLN*N 282
Score = 23.1 bits (44), Expect(4) = 1e-034
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +3 / +1
Query: 249 RIFGGNSRSGFRAVD*SIH 305
R G + FRAVD*SIH
Sbjct: 166 RYLVGRAGFRFRAVD*SIH 222
Score = 22.1 bits (42), Expect(3) = 9e-023
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -3 / -3
Query: 305 MDRSINCSEA 276
MD SINCSE+
Sbjct: 222 MDGSINCSES 193
Score = 18.9 bits (35), Expect(4) = 1e-034
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = +1 / +2
Query: 466 YYFRIEALV 492
YY++ EALV
Sbjct: 356 YYYKTEALV 382
>gb|CW376050.1|CW376050 fsbb001f053l16k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f053l16, DNA
sequence
Length = 746
Score = 79.0 bits (166), Expect(4) = 3e-027
Identities = 33/35 (94%), Positives = 34/35 (97%)
Frame = +3 / +2
Query: 168 DDYFFAALPVVQKRIAQGGVRLAAILNRIFGGNSR 272
DDYFFAALPVVQKRIAQGGVRLAAILN+IFGG SR
Sbjct: 317 DDYFFAALPVVQKRIAQGGVRLAAILNKIFGGKSR 421
Score = 54.7 bits (113), Expect(4) = 1e-021
Identities = 24/34 (70%), Positives = 26/34 (76%)
Frame = -3 / -2
Query: 269 AVPTKYPVEDRGQPDAPLRDPLLNHRQRGEEVVI 168
A+PTKY V GQPD PL +PLLNHRQ EEVVI
Sbjct: 418 ALPTKYLV*YCGQPDTPLSNPLLNHRQCSEEVVI 317
Score = 53.8 bits (111), Expect(4) = 1e-021
Identities = 20/26 (76%), Positives = 21/26 (80%)
Frame = -2 / -1
Query: 171 HLPRWSLGRHPRTPCRPASRSLQRTC 94
+L RW RHPRTPC PASRSLQRTC
Sbjct: 170 YLLRWIPARHPRTPCMPASRSLQRTC 93
Score = 53.3 bits (110), Expect(4) = 3e-027
Identities = 21/24 (87%), Positives = 24/24 (100%)
Frame = +3 / +2
Query: 96 KYAAESAKLACTAYEGVDQDSTLE 167
+YAAESAKLACTAYEGV+Q+STLE
Sbjct: 95 RYAAESAKLACTAYEGVEQESTLE 166
Score = 28.6 bits (56), Expect(4) = 1e-021
Identities = 10/14 (71%), Positives = 13/14 (92%)
Frame = -2 / -1
Query: 396 IILIQMADHACPSP 355
+ILIQ+ADHA P+P
Sbjct: 518 LILIQIADHAIPTP 477
Score = 23.5 bits (45), Expect(4) = 3e-027
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = +1 / +3
Query: 361 GAGMICHLN*N 393
G GM+C+LN*N
Sbjct: 483 GDGMVCNLN*N 515
Score = 23.1 bits (44), Expect(4) = 3e-027
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +3 / +3
Query: 249 RIFGGNSRSGFRAVD*SIH 305
R G + FRAVD*SIH
Sbjct: 399 RYLVGRAGFRFRAVD*SIH 455
Score = 22.1 bits (42), Expect(4) = 1e-021
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -3 / -1
Query: 305 MDRSINCSEA 276
MD SINCSE+
Sbjct: 455 MDGSINCSES 426
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 644,656,803
Number of Sequences: 832831
Number of extensions: 9806893
Number of successful extensions: 239923
Number of sequences better than 1.0e-020: 5
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 14596874586
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)