TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750663.2.1
(1282 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF587001.1|BF587001 FM1_32_A10.g1_A003 Floral-Induced Me... 224 6e-067
gb|CW399494.1|CW399494 fsbb001f089f22f0 Sorghum methylation... 151 3e-044
gb|CW089385.1|CW089385 104_434_10948678_115_32585_083 Sorgh... 122 5e-038
gb|CN126571.1|CN126571 RHOH1_18_A05.b1_A002 Acid- and alkal... 90 9e-029
>gb|BF587001.1|BF587001 FM1_32_A10.g1_A003 Floral-Induced Meristem 1 (FM1) Sorghum
propinquum cDNA, mRNA sequence
Length = 522
Score = 224 bits (483), Expect(2) = 6e-067
Identities = 92/103 (89%), Positives = 98/103 (95%)
Frame = -1 / +1
Query: 442 FCKLWLGVLSRMEKYVKVKVRGKRSDRLQEVVPDLLKNILLVMKSKGILAKRSTIGGDSL 263
FCKLWLGVLSRMEKY+K+KVRGKRSD+LQEV+PDLLKNILLVMK+KGILAKRSTIGGDSL
Sbjct: 1 FCKLWLGVLSRMEKYIKIKVRGKRSDKLQEVIPDLLKNILLVMKNKGILAKRSTIGGDSL 180
Query: 262 WELTWLHAHNISASLLPDVFPSQEYEQQSSTGSPRGPSPVEA* 134
WELTWLHA+NIS SLLPDVFPSQEYE QSS GSP GPS VEA*
Sbjct: 181 WELTWLHANNISTSLLPDVFPSQEYEHQSSAGSPXGPSSVEA* 309
Score = 185 bits (398), Expect(2) = 1e-057
Identities = 81/103 (78%), Positives = 82/103 (79%)
Frame = +2 / -1
Query: 134 LRLNRTGPSWATGXXXXXXXXXWKNIRQQTSRDVVRVKPRQLPQAVAPDRAPLRKDALAL 313
LRL RTGP ATG WKNIRQQTS DVVRVKPRQLPQAV P+RAPLRKDAL L
Sbjct: 309 LRLYRTGPXXATGTALMLILLTWKNIRQQTS*DVVRVKPRQLPQAVTPNRAPLRKDALVL 130
Query: 314 HHEQNVLEQVRDHLLQPVAPLASDLDLDVLLHPAQDPQPQLAE 442
HHE NVLEQVRDHLLQ V PLASDLDLDV LHPAQD QPQ AE
Sbjct: 129 HHE*NVLEQVRDHLLQLVTPLASDLDLDVFLHPAQDSQPQFAE 1
Score = 138 bits (296), Expect(2) = 4e-039
Identities = 63/86 (73%), Positives = 65/86 (75%)
Frame = +3 / -2
Query: 183 CSYSWLGKTSGSRLAEMLCA*SHVSSHRLSPPIVLLFARMPLLFITSRMFLSRSGXXXXX 362
CSYS LGKTSGS+L EML A*SHVSSH+LSPPIVLLFARMPL FITSRMFLSRSG
Sbjct: 260 CSYS*LGKTSGSKLVEMLFA*SHVSSHKLSPPIVLLFARMPLFFITSRMFLSRSGITSCS 81
Query: 363 XXXXXXXXXXXXYFSIRLRTPSHSLQ 440
YFSIRLRTPSHSLQ
Sbjct: 80 LSLRLPRTLILMYFSIRLRTPSHSLQ 3
Score = 114 bits (244), Expect(3) = 4e-052
Identities = 52/69 (75%), Positives = 54/69 (78%)
Frame = -3 / +3
Query: 329 HSARDEEQGHPCEEEHDRGRQPVGADVASRAQHLG*SAA*CFSKPGVRATEQHR*PKRAQ 150
HS RDEEQGHPCEEEHD G Q VGADVASR QHL * AA*CFSK GV A+EQ R*P RAQ
Sbjct: 114 HSTRDEEQGHPCEEEHDWG*QLVGADVASREQHLN*FAA*CFSKSGV*ASEQCR*PXRAQ 293
Query: 149 SC*GVAVNV 123
C GV + V
Sbjct: 294 FCRGVVLKV 320
Score = 84.0 bits (177), Expect(3) = 3e-023
Identities = 36/46 (78%), Positives = 38/46 (82%)
Frame = -2 / +2
Query: 264 CGS*RGFTRTTSRLVCCLMFFQARSTSNRAAPVAQEGPVLLRRSGQ 127
CGS*RGFTRTTS+LVCCLMFFQ RS S RA PVA GPVL RRS +
Sbjct: 179 CGS*RGFTRTTSQLVCCLMFFQVRSMSIRAVPVAXXGPVL*RRSAK 316
Score = 81.3 bits (171), Expect(3) = 4e-052
Identities = 32/39 (82%), Positives = 35/39 (89%)
Frame = -2 / +2
Query: 441 SASCGWGS*AGWRSTSRSRSEASGATGCRRWSRTCSRTF 325
SA+CGW S*AGWR+TSRSRSEASG T C+R SRTCSRTF
Sbjct: 2 SANCGWES*AGWRNTSRSRSEASGVTSCKR*SRTCSRTF 118
Score = 60.6 bits (126), Expect(2) = 1e-057
Identities = 24/31 (77%), Positives = 27/31 (87%)
Frame = +2 / -3
Query: 2 AXEAKRNHNIETLHFQWIRARAVVNLVKQIK 94
A EAK+N+NI+TLHFQWIRAR VNLVK IK
Sbjct: 457 AVEAKQNYNIKTLHFQWIRARVAVNLVKHIK 365
Score = 52.8 bits (109), Expect(2) = 6e-067
Identities = 22/28 (78%), Positives = 24/28 (85%)
Frame = -1 / +2
Query: 94 FYLFY*VYYCSCPNPLEV*GFDIVIAFR 11
FY+F *VY SCPNPL+V*GFDIVI FR
Sbjct: 365 FYVFN*VYCYSCPNPLKV*GFDIVILFR 448
Score = 50.1 bits (103), Expect(3) = 4e-052
Identities = 22/31 (70%), Positives = 23/31 (74%)
Frame = -2 / +3
Query: 93 FICFTRFTTARALIHWKCKVSIL*LRFASXA 1
F+C TRFT RALIH KCKV IL* FAS A
Sbjct: 366 FMCLTRFTATRALIH*KCKVLIL*FCFASTA 458
Score = 45.5 bits (93), Expect(2) = 4e-039
Identities = 22/29 (75%), Positives = 23/29 (79%)
Frame = +3 / -1
Query: 9 KRNAITISKPYTSNGLGHEQ**T**NK*N 95
+RN ITISKPYT NGLGHE *T* N *N
Sbjct: 450 RRNKITISKPYTFNGLGHE*Q*T*LNT*N 364
Score = 35.4 bits (71), Expect(3) = 3e-023
Identities = 13/18 (72%), Positives = 14/18 (77%)
Frame = -3 / +3
Query: 440 LQAVAGGPEPDGEVRQGQ 387
LQ VAG PEPDGE+ Q Q
Sbjct: 3 LQTVAGSPEPDGEIHQDQ 56
Score = 28.6 bits (56), Expect(3) = 3e-023
Identities = 13/18 (72%), Positives = 14/18 (77%)
Frame = -3 / +1
Query: 59 P*SIGSVRFRYCDCVSLP 6
P*SI SVRF YC+ VS P
Sbjct: 400 P*SIESVRF*YCNFVSPP 453
>gb|CW399494.1|CW399494 fsbb001f089f22f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f089f22, DNA
sequence
Length = 619
Score = 151 bits (324), Expect(2) = 3e-044
Identities = 63/102 (61%), Positives = 86/102 (84%)
Frame = -1 / +2
Query: 595 VIFSLLDDLLEISQNHSQKDYRNMEGSLVLAMKLVAKVYLQLLPDLFGLSSFCKLWLGVL 416
+IF LLD+LLEI+Q++S KD+RNME SL+ A+KL+ KV+LQ L DL G SF KLWL VL
Sbjct: 164 IIFQLLDELLEIAQSYSPKDFRNMEVSLLHAVKLLCKVFLQSLKDLSGQGSFDKLWLEVL 343
Query: 415 SRMEKYVKVKVRGKRSDRLQEVVPDLLKNILLVMKSKGILAK 290
+EK++KVKVRG+R+++LQE +P+L+KNIL+VMK+ GIL+K
Sbjct: 344 DMIEKFMKVKVRGRRTEKLQEAIPELVKNILMVMKASGILSK 469
Score = 49.6 bits (102), Expect(2) = 3e-044
Identities = 22/41 (53%), Positives = 23/41 (56%)
Frame = -1 / +2
Query: 763 EAIREMWXXXXXXXXXXXLDQREEVRNHALASLQRCLTSTG 641
E I EMW +DQREEVRNHAL SL RCL G
Sbjct: 2 EGIAEMWLRLVQALRKVCMDQREEVRNHALLSLHRCLVVDG 124
>gb|CW089385.1|CW089385 104_434_10948678_115_32585_083 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10948678, DNA
sequence
Length = 671
Score = 122 bits (262), Expect(3) = 5e-038
Identities = 51/55 (92%), Positives = 52/55 (94%)
Frame = -1 / +3
Query: 298 LAKRSTIGGDSLWELTWLHAHNISASLLPDVFPSQEYEQQSSTGSPRGPSPVEA* 134
LAKRSTIGGDSLWELTWLHA+NIS SLLPDVFPSQEYEQQSS GSPRGPS VEA*
Sbjct: 3 LAKRSTIGGDSLWELTWLHANNISTSLLPDVFPSQEYEQQSSAGSPRGPSSVEA* 167
Score = 99.6 bits (211), Expect(3) = 2e-032
Identities = 42/55 (76%), Positives = 43/55 (78%)
Frame = +2 / -1
Query: 134 LRLNRTGPSWATGXXXXXXXXXWKNIRQQTSRDVVRVKPRQLPQAVAPDRAPLRK 298
LRL RTGPSWATG WKNIRQQTS DVVRVKPRQLPQAV P+RAPLRK
Sbjct: 167 LRLYRTGPSWATGTALLLILLTWKNIRQQTS*DVVRVKPRQLPQAVTPNRAPLRK 3
Score = 95.5 bits (202), Expect(3) = 1e-028
Identities = 45/59 (76%), Positives = 46/59 (77%)
Frame = -3 / +2
Query: 299 PCEEEHDRGRQPVGADVASRAQHLG*SAA*CFSKPGVRATEQHR*PKRAQSC*GVAVNV 123
PCEEEHD G Q VGADVASR QHL * AA*CFSK GV ATEQ R*PKRAQ C GV + V
Sbjct: 2 PCEEEHDWG*QLVGADVASREQHLN*FAA*CFSKSGV*ATEQCR*PKRAQFCRGVVLKV 178
Score = 89.5 bits (189), Expect(3) = 5e-026
Identities = 36/47 (76%), Positives = 40/47 (85%)
Frame = +1 / -3
Query: 124 TLTATPQQDWALLGYRCCSVARTPGLEKHQAAD*PRCCAREATSAPT 264
T + TP Q+WALLGYR CSVA TP LEKHQAA+* RCC+REATSAPT
Sbjct: 177 TFSTTPLQNWALLGYRHCSVAHTPDLEKHQAAN*LRCCSREATSAPT 37
Score = 49.6 bits (102), Expect(3) = 2e-032
Identities = 20/23 (86%), Positives = 20/23 (86%)
Frame = +2 / -1
Query: 38 LHFQWIRARAVVNLVKQIKHTHT 106
LHFQWIRAR VVNLVK IK THT
Sbjct: 266 LHFQWIRARLVVNLVKHIKLTHT 198
Score = 48.3 bits (99), Expect(3) = 5e-038
Identities = 18/23 (78%), Positives = 20/23 (86%)
Frame = -2 / +1
Query: 105 VCVCFICFTRFTTARALIHWKCK 37
VCV F+C TRFTT+RALIH KCK
Sbjct: 199 VCVSFMCLTRFTTSRALIH*KCK 267
Score = 46.0 bits (94), Expect(3) = 1e-028
Identities = 18/23 (78%), Positives = 20/23 (86%)
Frame = -1 / +3
Query: 103 VCMFYLFY*VYYCSCPNPLEV*G 35
VC FY+F *VYY SCPNPL+V*G
Sbjct: 201 VCKFYVFN*VYY*SCPNPLKV*G 269
Score = 38.2 bits (77), Expect(3) = 5e-026
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = +3 / -2
Query: 36 PYTSNGLGHEQ**T**NK*NIHTRS 110
PYT NGLGH+ **T* N *N+HT +
Sbjct: 268 PYTFNGLGHD***T*LNT*NLHTHT 194
Score = 29.9 bits (59), Expect(3) = 5e-026
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = +2 / -1
Query: 2 AXEAKRNHNIETLHF 46
A EAK N+NI+TLHF
Sbjct: 332 AVEAKHNYNIKTLHF 288
Score = 29.9 bits (59), Expect(3) = 2e-032
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = +1 / -3
Query: 1 CRGSETQSQYRNLTL 45
C G ETQ QY+NLTL
Sbjct: 333 CSGGETQLQYQNLTL 289
Score = 27.2 bits (53), Expect(3) = 5e-038
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -3 / +2
Query: 44 SVRFRYCDCVSLP 6
SVRF YC+CVS P
Sbjct: 290 SVRF*YCNCVSPP 328
Score = 25.4 bits (49), Expect(3) = 1e-028
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = -2 / +1
Query: 45 KCKVSIL*LRFASXA 1
KCKV IL*L FAS A
Sbjct: 289 KCKVLIL*LCFASTA 333
>gb|CN126571.1|CN126571 RHOH1_18_A05.b1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_18_A05_A002 3', mRNA sequence
Length = 749
Score = 89.5 bits (189), Expect(2) = 9e-029
Identities = 36/60 (60%), Positives = 49/60 (81%)
Frame = -1 / +3
Query: 469 LPDLFGLSSFCKLWLGVLSRMEKYVKVKVRGKRSDRLQEVVPDLLKNILLVMKSKGILAK 290
L DL G SF KLWL VL +EK++ VKVRG+R+++LQE +P+L+KNIL+VMK+ GIL+K
Sbjct: 168 LKDLSGQGSFDKLWLEVLDMIEKFMNVKVRGRRTEKLQEAIPELVKNILMVMKASGILSK 347
Score = 59.7 bits (124), Expect(2) = 9e-029
Identities = 24/41 (58%), Positives = 34/41 (82%)
Frame = -1 / +2
Query: 595 VIFSLLDDLLEISQNHSQKDYRNMEGSLVLAMKLVAKVYLQ 473
+IF LLD+LLEI+Q++S KD+RNME S + A+KL+ K +LQ
Sbjct: 41 IIFQLLDELLEIAQSYSPKDFRNMEVSFLHAVKLLCKAFLQ 163
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,653,246,840
Number of Sequences: 832831
Number of extensions: 26177173
Number of successful extensions: 181970
Number of sequences better than 1.0e-020: 5
length of database: 163,786,556
effective HSP length: 56
effective length of database: 117,148,020
effective search space used: 43344767400
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)