TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2568371.2.1
(782 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW565409.1|AW565409 LG1_343_G07.g1_A002 Light Grown 1 (L... 108 2e-048
gb|CD427188.1|CD427188 SA1_18_F03.b1_A002 Salicylic acid-tr... 38 3e-021
>gb|AW565409.1|AW565409 LG1_343_G07.g1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 646
Score = 108 bits (231), Expect(7) = 1e-042
Identities = 41/42 (97%), Positives = 42/42 (100%)
Frame = +2 / +1
Query: 74 FWVLFHLYPFAKGILGRHGKTPVVVLVWWAFTFVITAVLYIN 199
FWVLFHLYPFAKG+LGRHGKTPVVVLVWWAFTFVITAVLYIN
Sbjct: 1 FWVLFHLYPFAKGLLGRHGKTPVVVLVWWAFTFVITAVLYIN 126
Score = 100 bits (212), Expect(9) = 2e-048
Identities = 41/45 (91%), Positives = 41/45 (91%)
Frame = -3 / -3
Query: 210 MWGMLMYSTAVMTKVKAHQTSTTTGVLPCLPRMPLAKG*RWNSTQ 76
MWGMLM STAVMTKV AHQTSTTTGVLPCLPR PLA G*RWNSTQ
Sbjct: 137 MWGMLM*STAVMTKVNAHQTSTTTGVLPCLPRRPLANG*RWNSTQ 3
Score = 44.1 bits (90), Expect(9) = 2e-048
Identities = 21/31 (67%), Positives = 23/31 (74%)
Frame = -2 / -2
Query: 331 GWLLRSIDPSRPEVVAVELLAMVGGAVLSDR 239
GWL + P EVV VELLA+VGGAVLSDR
Sbjct: 258 GWLPGASRPVVGEVVEVELLAVVGGAVLSDR 166
Score = 35.4 bits (71), Expect(7) = 1e-042
Identities = 13/18 (72%), Positives = 13/18 (72%)
Frame = +3 / +3
Query: 318 RSSQPSIVMHAHTCPDDD 371
R SIV HAHTCPDDD
Sbjct: 261 RRHPASIVKHAHTCPDDD 314
Score = 33.1 bits (66), Expect(9) = 2e-048
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -1 / -3
Query: 515 WRVRRWVDQMCVGWCV 468
WRVRRWVDQM G V
Sbjct: 458 WRVRRWVDQMLCGVAV 411
Score = 32.7 bits (65), Expect(9) = 2e-048
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -3 / -2
Query: 372 YHHQGRYVHA*RCWVG 325
+HHQGRYVHA*R +G
Sbjct: 315 HHHQGRYVHA*R*MLG 268
Score = 29.9 bits (59), Expect(7) = 1e-042
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = +2 / +3
Query: 566 FLLVLSRN*KMC*DVLI 616
FLLVLSRN*KMC* L+
Sbjct: 519 FLLVLSRN*KMC*FDLV 569
Score = 28.1 bits (55), Expect(7) = 1e-042
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +3 / +1
Query: 657 LCTYLYIHY 683
LCTYLYIHY
Sbjct: 598 LCTYLYIHY 624
Score = 28.1 bits (55), Expect(7) = 1e-042
Identities = 10/11 (90%), Positives = 11/11 (100%)
Frame = +2 / +1
Query: 626 FFNYRTRGN*I 658
FFNYRTRGN*+
Sbjct: 568 FFNYRTRGN*L 600
Score = 26.7 bits (52), Expect(7) = 1e-042
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +1 / +1
Query: 466 HTHQPTHI*STQRRTRQ 516
+T P I*STQRRTRQ
Sbjct: 409 YTATPHSI*STQRRTRQ 459
Score = 26.7 bits (52), Expect(9) = 2e-048
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -1 / -1
Query: 653 NYHGYGN*K 627
NYHGYGN*K
Sbjct: 595 NYHGYGN*K 569
Score = 24.4 bits (47), Expect(7) = 1e-042
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +3 / +2
Query: 270 ARSSTATTSGREGSIDRSSQP 332
ARSST+TTS G SQP
Sbjct: 197 ARSSTSTTSPTTGREAPGSQP 259
Score = 24.4 bits (47), Expect(9) = 2e-048
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = -2 / -3
Query: 601 THLSISGQN* 572
THLSISGQN*
Sbjct: 554 THLSISGQN* 525
Score = 24.0 bits (46), Expect(9) = 2e-048
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -3 / -2
Query: 633 LKKLNQINTS 604
+KKLNQINTS
Sbjct: 576 IKKLNQINTS 547
Score = 23.5 bits (45), Expect(9) = 2e-048
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -2 / -3
Query: 682 *CIYK*VHN 656
*CIYK*VHN
Sbjct: 623 *CIYK*VHN 597
Score = 21.7 bits (41), Expect(9) = 2e-048
Identities = 7/12 (58%), Positives = 8/12 (66%)
Frame = -2 / -2
Query: 475 GVCVFFTLHHQL 440
G CV FTLH +
Sbjct: 420 GCCVVFTLHQPI 385
>gb|CD427188.1|CD427188 SA1_18_F03.b1_A002 Salicylic acid-treated seedlings Sorghum bicolor
cDNA clone SA1_18_F03_A002 3', mRNA sequence
Length = 489
Score = 38.2 bits (77), Expect(7) = 3e-021
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = -2 / -2
Query: 295 EVVAVELLAMVGGAVLSDR 239
EVV VELLA+VGGAVLSDR
Sbjct: 122 EVVEVELLAVVGGAVLSDR 66
Score = 33.1 bits (66), Expect(7) = 3e-021
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -1 / -3
Query: 515 WRVRRWVDQMCVGWCV 468
WRVRRWVDQM G V
Sbjct: 361 WRVRRWVDQMLCGVAV 314
Score = 32.7 bits (65), Expect(7) = 3e-021
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -3 / -2
Query: 372 YHHQGRYVHA*RCWVG 325
+HHQGRYVHA*R +G
Sbjct: 218 HHHQGRYVHA*R*MLG 171
Score = 29.0 bits (57), Expect(7) = 3e-021
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -3 / -3
Query: 210 MWGMLMYSTAVM 175
MWGMLM STAVM
Sbjct: 37 MWGMLMXSTAVM 2
Score = 24.4 bits (47), Expect(7) = 3e-021
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = -2 / -3
Query: 601 THLSISGQN* 572
THLSISGQN*
Sbjct: 457 THLSISGQN* 428
Score = 24.0 bits (46), Expect(7) = 3e-021
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -3 / -2
Query: 633 LKKLNQINTS 604
+KKLNQINTS
Sbjct: 479 IKKLNQINTS 450
Score = 21.7 bits (41), Expect(7) = 3e-021
Identities = 7/12 (58%), Positives = 8/12 (66%)
Frame = -2 / -2
Query: 475 GVCVFFTLHHQL 440
G CV FTLH +
Sbjct: 323 GCCVVFTLHQPI 288
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 798,375,479
Number of Sequences: 832831
Number of extensions: 10109590
Number of successful extensions: 96345
Number of sequences better than 1.0e-020: 3
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 24475618492
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)