TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2494085.2.2
(660 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG049431.1|BG049431 OV1_19_H02.g1_A002 Ovary 1 (OV1) Sor... 140 2e-033
gb|BG240504.1|BG240504 OV1_30_G12.b1_A002 Ovary 1 (OV1) Sor... 139 4e-033
gb|BG240262.1|BG240262 OV1_19_H02.b1_A002 Ovary 1 (OV1) Sor... 140 2e-032
gb|CW042188.1|CW042188 104_276_10506979_114_30482 Sorghum m... 130 2e-030
gb|CW260825.1|CW260825 104_727_11229176_116_35196_020 Sorgh... 130 2e-030
gb|BZ350671.1|BZ350671 ht59c02.g1 WGS-SbicolorF (JM107 adap... 130 2e-030
gb|CW487350.1|CW487350 fsbb001f253c16f0 Sorghum methylation... 129 4e-030
gb|CL157218.1|CL157218 104_344_10783391_116_31374_335 Sorgh... 129 4e-030
gb|CL180693.1|CL180693 104_390_10896198_116_31930_342 Sorgh... 120 2e-026
gb|CW260826.1|CW260826 104_727_11229176_148_35200_020 Sorgh... 106 3e-023
gb|CW092637.1|CW092637 104_455_10999283_114_33047_044 Sorgh... 109 5e-023
>gb|BG049431.1|BG049431 OV1_19_H02.g1_A002 Ovary 1 (OV1) Sorghum bicolor cDNA, mRNA
sequence
Length = 344
Score = 140 bits (301), Expect(2) = 2e-033
Identities = 53/71 (74%), Positives = 60/71 (84%)
Frame = -2 / +2
Query: 335 VVLLLLVCYTGTQGGPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLR 156
V+LLLL+ Y GP+TM ARKCES S +F+GPCSRDANCA+VCLTEGFTGG CKGLR
Sbjct: 59 VLLLLLIGYNTETQGPITMALARKCESPSLKFRGPCSRDANCASVCLTEGFTGGECKGLR 238
Query: 155 HRCFCTRDC*Y 123
HRCFCTRDC*+
Sbjct: 239 HRCFCTRDC*F 271
Score = 110 bits (234), Expect(2) = 5e-024
Identities = 46/56 (82%), Positives = 49/56 (87%)
Frame = +1 / -3
Query: 127 *QSLVQKQRWRRPLHTPPVKPSVRQTFAQLASLEQGPLKRKLCDSHFLADTIVTGP 294
*QSLVQKQRWRRPLH+PPVKPSVRQT AQ ASLEQGPL +L DSHFLA+ IV GP
Sbjct: 267 *QSLVQKQRWRRPLHSPPVKPSVRQTLAQFASLEQGPLNLRLGDSHFLANAIVIGP 100
Score = 22.6 bits (43), Expect(2) = 2e-033
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -3 / +3
Query: 112 RVLRVHI*PQRISKTCM 62
RVL + P RI K CM
Sbjct: 276 RVLHAYACPNRIRKICM 326
Score = 21.7 bits (41), Expect(2) = 5e-024
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +3 / -2
Query: 63 MHVLLIRCGYICTRRTR 113
+H+ LIR G+ RTR
Sbjct: 325 IHIFLIRLGHAYACRTR 275
>gb|BG240504.1|BG240504 OV1_30_G12.b1_A002 Ovary 1 (OV1) Sorghum bicolor cDNA, mRNA
sequence
Length = 429
Score = 139 bits (298), Expect(2) = 4e-033
Identities = 54/71 (76%), Positives = 60/71 (84%)
Frame = -2 / +1
Query: 338 IVVLLLLVCYTGTQGGPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGL 159
+++LLL+ YT TQ GPVTM ARKCES S RF+GPCSRDANCA VC TEGFTGG CKGL
Sbjct: 67 VLLLLLIGGYTDTQLGPVTMALARKCESPSHRFQGPCSRDANCATVCRTEGFTGGKCKGL 246
Query: 158 RHRCFCTRDC* 126
RHRCFCT+DC*
Sbjct: 247 RHRCFCTKDC* 279
Score = 122 bits (260), Expect = 7e-027
Identities = 55/71 (77%), Positives = 55/71 (77%)
Frame = +1 / -2
Query: 118 RQY*QSLVQKQRWRRPLHTPPVKPSVRQTFAQLASLEQGPLKRKLCDSHFLADTIVTGPP 297
R *QSLVQKQRWRRPLH PPVKPSVRQT AQ ASLEQGP KR L DSHFLA IVTGP
Sbjct: 287 RGN*QSLVQKQRWRRPLHLPPVKPSVRQTVAQFASLEQGPWKRWLGDSHFLASAIVTGPS 108
Query: 298 CVPV*QTSRRR 330
CV V* R R
Sbjct: 107 CVSV*PPMRSR 75
Score = 23.1 bits (44), Expect(2) = 4e-033
Identities = 7/21 (33%), Positives = 13/21 (61%)
Frame = -1 / +2
Query: 108 SCVCIYSRSGSVKHACMRYQI 46
SC+ +Y+ ++ CM YQ+
Sbjct: 296 SCMHMYACPERIRRICMHYQM 358
>gb|BG240262.1|BG240262 OV1_19_H02.b1_A002 Ovary 1 (OV1) Sorghum bicolor cDNA, mRNA
sequence
Length = 284
Score = 140 bits (301), Expect = 2e-032
Identities = 53/71 (74%), Positives = 60/71 (84%)
Frame = -2 / +2
Query: 335 VVLLLLVCYTGTQGGPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLR 156
V+LLLL+ Y GP+TM ARKCES S +F+GPCSRDANCA+VCLTEGFTGG CKGLR
Sbjct: 59 VLLLLLIGYNTETQGPITMALARKCESPSLKFRGPCSRDANCASVCLTEGFTGGECKGLR 238
Query: 155 HRCFCTRDC*Y 123
HRCFCTRDC*+
Sbjct: 239 HRCFCTRDC*F 271
Score = 110 bits (234), Expect = 3e-023
Identities = 46/56 (82%), Positives = 49/56 (87%)
Frame = +1 / -3
Query: 127 *QSLVQKQRWRRPLHTPPVKPSVRQTFAQLASLEQGPLKRKLCDSHFLADTIVTGP 294
*QSLVQKQRWRRPLH+PPVKPSVRQT AQ ASLEQGPL +L DSHFLA+ IV GP
Sbjct: 267 *QSLVQKQRWRRPLHSPPVKPSVRQTLAQFASLEQGPLNLRLGDSHFLANAIVIGP 100
>gb|CW042188.1|CW042188 104_276_10506979_114_30482 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10506979, DNA
sequence
Length = 670
Score = 130 bits (279), Expect(2) = 2e-030
Identities = 47/57 (82%), Positives = 52/57 (91%)
Frame = -2 / +3
Query: 293 GPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLRHRCFCTRDC*Y 123
GP+TM ARKCES S +F+GPCSRDANCA+VCLTEGFTGG CKGLRHRCFCTRDC*+
Sbjct: 132 GPITMALARKCESPSLKFRGPCSRDANCASVCLTEGFTGGECKGLRHRCFCTRDC*F 302
Score = 110 bits (234), Expect(2) = 2e-023
Identities = 46/56 (82%), Positives = 49/56 (87%)
Frame = +1 / -1
Query: 127 *QSLVQKQRWRRPLHTPPVKPSVRQTFAQLASLEQGPLKRKLCDSHFLADTIVTGP 294
*QSLVQKQRWRRPLH+PPVKPSVRQT AQ ASLEQGPL +L DSHFLA+ IV GP
Sbjct: 298 *QSLVQKQRWRRPLHSPPVKPSVRQTLAQFASLEQGPLNLRLGDSHFLANAIVIGP 131
Score = 22.6 bits (43), Expect(2) = 2e-030
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -3 / +1
Query: 112 RVLRVHI*PQRISKTCM 62
RVL + P RI K CM
Sbjct: 307 RVLHAYACPNRIRKICM 357
Score = 21.7 bits (41), Expect(2) = 2e-023
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +3 / -3
Query: 63 MHVLLIRCGYICTRRTR 113
+H+ LIR G+ RTR
Sbjct: 356 IHIFLIRLGHAYACRTR 306
>gb|CW260825.1|CW260825 104_727_11229176_116_35196_020 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11229176, DNA
sequence
Length = 654
Score = 130 bits (279), Expect(2) = 2e-030
Identities = 47/57 (82%), Positives = 52/57 (91%)
Frame = -2 / +1
Query: 293 GPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLRHRCFCTRDC*Y 123
GP+TM ARKCES S +F+GPCSRDANCA+VCLTEGFTGG CKGLRHRCFCTRDC*+
Sbjct: 247 GPITMALARKCESPSLKFRGPCSRDANCASVCLTEGFTGGECKGLRHRCFCTRDC*F 417
Score = 110 bits (234), Expect(2) = 2e-023
Identities = 46/56 (82%), Positives = 49/56 (87%)
Frame = +1 / -2
Query: 127 *QSLVQKQRWRRPLHTPPVKPSVRQTFAQLASLEQGPLKRKLCDSHFLADTIVTGP 294
*QSLVQKQRWRRPLH+PPVKPSVRQT AQ ASLEQGPL +L DSHFLA+ IV GP
Sbjct: 413 *QSLVQKQRWRRPLHSPPVKPSVRQTLAQFASLEQGPLNLRLGDSHFLANAIVIGP 246
Score = 22.6 bits (43), Expect(2) = 2e-030
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -3 / +2
Query: 112 RVLRVHI*PQRISKTCM 62
RVL + P RI K CM
Sbjct: 422 RVLHAYACPNRIRKICM 472
Score = 21.7 bits (41), Expect(2) = 2e-023
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +3 / -1
Query: 63 MHVLLIRCGYICTRRTR 113
+H+ LIR G+ RTR
Sbjct: 471 IHIFLIRLGHAYACRTR 421
>gb|BZ350671.1|BZ350671 ht59c02.g1 WGS-SbicolorF (JM107 adapted methyl filtered) Sorghum
bicolor genomic clone ht59c02 5', DNA sequence
Length = 551
Score = 130 bits (279), Expect(2) = 2e-030
Identities = 47/57 (82%), Positives = 52/57 (91%)
Frame = -2 / -1
Query: 293 GPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLRHRCFCTRDC*Y 123
GP+TM ARKCES S +F+GPCSRDANCA+VCLTEGFTGG CKGLRHRCFCTRDC*+
Sbjct: 290 GPITMALARKCESPSLKFRGPCSRDANCASVCLTEGFTGGECKGLRHRCFCTRDC*F 120
Score = 110 bits (234), Expect(2) = 2e-023
Identities = 46/56 (82%), Positives = 49/56 (87%)
Frame = +1 / +1
Query: 127 *QSLVQKQRWRRPLHTPPVKPSVRQTFAQLASLEQGPLKRKLCDSHFLADTIVTGP 294
*QSLVQKQRWRRPLH+PPVKPSVRQT AQ ASLEQGPL +L DSHFLA+ IV GP
Sbjct: 124 *QSLVQKQRWRRPLHSPPVKPSVRQTLAQFASLEQGPLNLRLGDSHFLANAIVIGP 291
Score = 22.6 bits (43), Expect(2) = 2e-030
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -3 / -2
Query: 112 RVLRVHI*PQRISKTCM 62
RVL + P RI K CM
Sbjct: 115 RVLHAYACPNRIRKICM 65
Score = 21.7 bits (41), Expect(2) = 2e-023
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +3 / +3
Query: 63 MHVLLIRCGYICTRRTR 113
+H+ LIR G+ RTR
Sbjct: 66 IHIFLIRLGHAYACRTR 116
>gb|CW487350.1|CW487350 fsbb001f253c16f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f253c16, DNA
sequence
Length = 813
Score = 129 bits (276), Expect(2) = 4e-030
Identities = 49/62 (79%), Positives = 52/62 (83%)
Frame = -2 / +1
Query: 311 YTGTQGGPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLRHRCFCTRD 132
+ TQ GPVTM ARKCES S RF+GPCSRDANCA VC TEGFTGG CKGLRHRCFCT+D
Sbjct: 145 FADTQLGPVTMALARKCESPSHRFQGPCSRDANCATVCRTEGFTGGKCKGLRHRCFCTKD 324
Query: 131 C* 126
C*
Sbjct: 325 C* 330
Score = 117 bits (250), Expect = 2e-025
Identities = 51/62 (82%), Positives = 51/62 (82%)
Frame = +1 / -2
Query: 118 RQY*QSLVQKQRWRRPLHTPPVKPSVRQTFAQLASLEQGPLKRKLCDSHFLADTIVTGPP 297
R *QSLVQKQRWRRPLH PPVKPSVRQT AQ ASLEQGP KR L DSHFLA IVTGP
Sbjct: 338 RGN*QSLVQKQRWRRPLHLPPVKPSVRQTVAQFASLEQGPWKRWLGDSHFLASAIVTGPS 159
Query: 298 CV 303
CV
Sbjct: 158 CV 153
Score = 23.1 bits (44), Expect(2) = 4e-030
Identities = 7/21 (33%), Positives = 13/21 (61%)
Frame = -1 / +2
Query: 108 SCVCIYSRSGSVKHACMRYQI 46
SC+ +Y+ ++ CM YQ+
Sbjct: 347 SCMHMYACPERIRRICMHYQM 409
>gb|CL157218.1|CL157218 104_344_10783391_116_31374_335 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10783391, DNA
sequence
Length = 726
Score = 129 bits (276), Expect(2) = 4e-030
Identities = 49/62 (79%), Positives = 52/62 (83%)
Frame = -2 / -2
Query: 311 YTGTQGGPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLRHRCFCTRD 132
+ TQ GPVTM ARKCES S RF+GPCSRDANCA VC TEGFTGG CKGLRHRCFCT+D
Sbjct: 293 FADTQLGPVTMALARKCESPSHRFQGPCSRDANCATVCRTEGFTGGKCKGLRHRCFCTKD 114
Query: 131 C* 126
C*
Sbjct: 113 C* 108
Score = 117 bits (250), Expect = 2e-025
Identities = 51/62 (82%), Positives = 51/62 (82%)
Frame = +1 / +1
Query: 118 RQY*QSLVQKQRWRRPLHTPPVKPSVRQTFAQLASLEQGPLKRKLCDSHFLADTIVTGPP 297
R *QSLVQKQRWRRPLH PPVKPSVRQT AQ ASLEQGP KR L DSHFLA IVTGP
Sbjct: 100 RGN*QSLVQKQRWRRPLHLPPVKPSVRQTVAQFASLEQGPWKRWLGDSHFLASAIVTGPS 279
Query: 298 CV 303
CV
Sbjct: 280 CV 285
Score = 23.1 bits (44), Expect(2) = 4e-030
Identities = 7/21 (33%), Positives = 13/21 (61%)
Frame = -1 / -3
Query: 108 SCVCIYSRSGSVKHACMRYQI 46
SC+ +Y+ ++ CM YQ+
Sbjct: 91 SCMHMYACPERIRRICMHYQM 29
>gb|CL180693.1|CL180693 104_390_10896198_116_31930_342 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10896198, DNA
sequence
Length = 484
Score = 120 bits (257), Expect = 2e-026
Identities = 43/55 (78%), Positives = 48/55 (87%)
Frame = -2 / +3
Query: 293 GPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLRHRCFCTRDC 129
GPVT+ ARKC+S+SF+FKGPCSRD NCA VCLTEGFT G C+GLR RCFCTRDC
Sbjct: 291 GPVTVALARKCQSKSFKFKGPCSRDENCATVCLTEGFTSGKCRGLRRRCFCTRDC 455
>gb|CW260826.1|CW260826 104_727_11229176_148_35200_020 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11229176, DNA
sequence
Length = 548
Score = 106 bits (227), Expect(2) = 3e-023
Identities = 38/45 (84%), Positives = 42/45 (93%)
Frame = -2 / -3
Query: 257 SQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLRHRCFCTRDC*Y 123
S S +F+GPCSRDANCA+VCLTEGFTGG CKGLRHRCFCTRDC*+
Sbjct: 546 SPSLKFRGPCSRDANCASVCLTEGFTGGECKGLRHRCFCTRDC*F 412
Score = 22.6 bits (43), Expect(2) = 3e-023
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -3 / -1
Query: 112 RVLRVHI*PQRISKTCM 62
RVL + P RI K CM
Sbjct: 407 RVLHAYACPNRIRKICM 357
>gb|CW092637.1|CW092637 104_455_10999283_114_33047_044 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10999283, DNA
sequence
Length = 700
Score = 109 bits (232), Expect = 5e-023
Identities = 39/51 (76%), Positives = 44/51 (86%)
Frame = -2 / +1
Query: 293 GPVTMVSARKCESQSFRFKGPCSRDANCANVCLTEGFTGGVCKGLRHRCFC 141
GPVT+ ARKC+S+SF+FKGPCSRD NCA VCLTEGFT G C+GLR RCFC
Sbjct: 547 GPVTVALARKCQSKSFKFKGPCSRDENCATVCLTEGFTSGKCRGLRRRCFC 699
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 788,829,525
Number of Sequences: 832831
Number of extensions: 11343940
Number of successful extensions: 113638
Number of sequences better than 1.0e-020: 12
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 19604257530
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)