TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2493528.2.1
(627 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX611195.1|CX611195 ANR1_23_E04.b1_A002 Anaerobic roots ... 417 e-115
>gb|CX611195.1|CX611195 ANR1_23_E04.b1_A002 Anaerobic roots Sorghum bicolor cDNA clone
ANR1_23_E04_A002 3', mRNA sequence
Length = 767
Score = 417 bits (904), Expect = e-115
Identities = 188/198 (94%), Positives = 192/198 (96%)
Frame = -2 / +1
Query: 617 QSQMIAEDRSLLLEKEYRNNILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQ 438
QSQMI EDRS LLEKEYRN+ILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQ
Sbjct: 124 QSQMITEDRSRLLEKEYRNDILDDLLEVKSFLTQRLGEMRNADTSSLQHQVQAVSPFVLQ 303
Query: 437 QHAPDSLENMLVEISSAISLLTNQKTLDLIMILNSKRFLDRLVSSLEEKKHHEVKLREGL 258
QHAPDSLENMLVE+SSAISLLTNQKTLDLIMILNSKRFLDRLVS+LEEKKHHEVKLREGL
Sbjct: 304 QHAPDSLENMLVEVSSAISLLTNQKTLDLIMILNSKRFLDRLVSTLEEKKHHEVKLREGL 483
Query: 257 GDLSVKRMELQNALSSSWPKQEAAITKTRELKKLCESTLSSVFDGRPVYIIGEINTLLSS 78
GDLSVKRMELQNALSSSWPKQEAAITKTRELKK CESTLSSVFDGRPV+IIGEINTLLSS
Sbjct: 484 GDLSVKRMELQNALSSSWPKQEAAITKTRELKKPCESTLSSVFDGRPVHIIGEINTLLSS 663
Query: 77 SVSQLAG*LYFRSSTK*D 24
SVSQLA *LYF S TK*D
Sbjct: 664 SVSQLAV*LYFCSWTK*D 717
Score = 370 bits (802), Expect = e-101
Identities = 168/201 (83%), Positives = 180/201 (89%)
Frame = -1 / +3
Query: 621 SSVSNDS*R*KFVAGERI*E*YSG*FTRGKIIFDSTLRGDEECRHIISTASSASCLTFCS 442
++VSND+*R*K V G+ I*E*+SG*FT GKIIFD+TLRGDEECR+IISTASSASCLTFCS
Sbjct: 120 TTVSNDN*R*KSVVGKGI*E*HSG*FT*GKIIFDTTLRGDEECRYIISTASSASCLTFCS 299
Query: 441 PAACS*QFGEHACRDLVSNLIAN*SEDS*PYNDP*L*KVSG*ISFKLRREEASRSKTTGR 262
PAACS* FGE+ACR SNLIAN*SEDS*PYNDP* *KVSG IS LRREEAS TGR
Sbjct: 300 PAACS**FGEYACRGFFSNLIAN*SEDS*PYNDP*F*KVSGQISVNLRREEAS*G*ITGR 479
Query: 261 FR*FIC*AYGAAKRTVIIMAKARGSNYKNKGTKEAV*VDTVVCF*WKTSVYNWRDQYFTE 82
FR*FIC*AYGAAKRTVIIMAKARGSNYKNKGTKEAV*V+T+VCF*WK S YNWRDQ+FTE
Sbjct: 480 FR*FIC*AYGAAKRTVIIMAKARGSNYKNKGTKEAV*VNTIVCF*WKASAYNWRDQHFTE 659
Query: 81 LKC*SISWMTVFSFLDQIRLQ 19
LKC*SIS MTVF FLDQIR +
Sbjct: 660 LKC*SISCMTVFLFLDQIRFE 722
Score = 347 bits (751), Expect = 1e-094
Identities = 155/199 (77%), Positives = 165/199 (82%)
Frame = +1 / -1
Query: 19 LQSYLVEERKYSHPAN*LTLELSKVLISPIIYTGLPSKTXXXXXXXXXXXXXXXXIAASC 198
L+SYLV+E+KYSH AN*LTLELSKVLISPII TGLPSKT IAASC
Sbjct: 722 LKSYLVQEQKYSHTAN*LTLELSKVLISPIICTGLPSKTDDSVDSHGFFSSLVFVIAASC 543
Query: 199 FGHDDDSAFCSSIRLTDKSPKPXXXXXXXXXXXXKLETNLSRNLLELRIIIRSRVF*LVS 378
FGHDDDSAFCSSIRLTDKSPKP K++TNLSRNLLELRIIIRSRVF*LVS
Sbjct: 542 FGHDDDSAFCSSIRLTDKSPKPSRNLTS*CFFSSKVDTNLSRNLLELRIIIRSRVF*LVS 363
Query: 379 NEIADEISTSMFSKLSGACCWRTKGETACT*CCRDDVSAFLISPKR*VKNDFTSSKSSRI 558
NEIA+E STS+FSKLSGACCWRTKGETACT*CCRDDVSAFLISPKR VKNDFTSSKSSR+
Sbjct: 362 NEIAEETSTSIFSKLSGACCWRTKGETACT*CCRDDVSAFLISPKRCVKNDFTSSKSSRM 183
Query: 559 LFLYSFSSNKLLSSAII*D 615
FLYSFS+N+LLSS II*D
Sbjct: 182 SFLYSFSNNRLLSSVII*D 126
Score = 273 bits (591), Expect(2) = 3e-094
Identities = 122/139 (87%), Positives = 132/139 (94%)
Frame = +3 / -3
Query: 201 WP***QCVLQLHTLNR*IT*TFP*FYFVMLLLF*A*N*SIQKPFRVKDHYKVKSLLIS*Q 380
WP***QCVLQLHTLNR*IT*TFP*F +MLLLF* *+*S+QKPFR+KDHYKVKSLLIS*Q
Sbjct: 540 WP***QCVLQLHTLNR*IT*TFP*FNLMMLLLF*G*H*SVQKPFRIKDHYKVKSLLIS*Q 361
Query: 381 *DC*RDLYKHVLQTVRSMLLENKR*DSLHLML*R*CVCIPHLP*ALSQK*FYLE*IIQNI 560
*DC*R+LYKH+LQT+RSMLLENKR*DSLHLML*R*C+CIPHLP*AL QK*FYL+*IIQN+
Sbjct: 360 *DC*RNLYKHILQTIRSMLLENKR*DSLHLML*R*CICIPHLP*ALCQK*FYLK*IIQNV 181
Query: 561 IPIFFLQQQTSIFSYHLRL 617
IPIF QQ TSIFSYHLRL
Sbjct: 180 IPIFLFQQPTSIFSYHLRL 124
Score = 247 bits (534), Expect(2) = 3e-083
Identities = 115/137 (83%), Positives = 121/137 (88%)
Frame = +2 / -2
Query: 206 MMMTVRFAAPYA*QINHLNLPVVLLRDASSLLSLKLIYPETF*S*GSL*GQESSD*LAMR 385
MMMTVRFAAPYA*QINHLNLPV+ DASSLL L LI PETF*+*GSL*GQESSD*LAMR
Sbjct: 535 MMMTVRFAAPYA*QINHLNLPVI*PHDASSLLRLTLICPETF*N*GSL*GQESSD*LAMR 356
Query: 386 LLTRSLQACSPNCQEHAAGEQKVRQLALDAVEMMCLHSSSPLSVESKMILPRVNHPEYYS 565
LL + LQA SPN QEHAAGEQKVRQLALDAVEMM LHSSSPLSV SKMILP+VNHPE +S
Sbjct: 355 LLKKPLQAYSPNYQEHAAGEQKVRQLALDAVEMMYLHSSSPLSVVSKMILPQVNHPECHS 176
Query: 566 YILSPATNFYLQLSFET 616
YI P T+FYLQLSFET
Sbjct: 175 YIPFPTTDFYLQLSFET 125
Score = 204 bits (441), Expect(2) = 9e-085
Identities = 90/103 (87%), Positives = 92/103 (89%)
Frame = -3 / +2
Query: 331 KGFWID*FQA*KRRSITK*NYGKV*VIYLLSVWSCKTHCHHHGQSKRQQLQKQGN*RSCV 152
KGFW D* Q *KRRSI + NYGKV*VIYLLSVWSCKTHCHHHGQSKRQQLQKQGN*RS V
Sbjct: 410 KGFWTD*CQP*KRRSIMRLNYGKV*VIYLLSVWSCKTHCHHHGQSKRQQLQKQGN*RSRV 589
Query: 151 SRHCRLFLMEDQCI*LERSILY*AQVLVN*LDDCIFVPRPNKI 23
S+H RLFLME QCI*LERS LY*AQVLVN*L DCIFVP PNKI
Sbjct: 590 SQHYRLFLMEGQCI*LERSTLY*AQVLVN*LYDCIFVPGPNKI 718
Score = 130 bits (278), Expect(2) = 9e-085
Identities = 58/81 (71%), Positives = 61/81 (75%)
Frame = -3 / +2
Query: 616 SLK**LKIEVCCWRKNIGIIFWMIYSR*NHF*LNA*GR*GMQTHHLYSIKCKXXXXXXXX 437
SLK**LKIEV CW++NIG+ FWMIY R*NHF* NA*GR*GMQ HHLYSIKCK
Sbjct: 125 SLK**LKIEVGCWKRNIGMTFWMIYLR*NHF*HNA*GR*GMQIHHLYSIKCKLSHLLFSS 304
Query: 436 XXXLTVWRTCL*RSRQQSHC* 374
L VWR CL*R QQSHC*
Sbjct: 305 SMLLIVWRICL*RFLQQSHC* 367
Score = 93.2 bits (197), Expect(2) = 3e-094
Identities = 41/47 (87%), Positives = 42/47 (89%)
Frame = +2 / -2
Query: 23 NLIWSRNENTVIQLID*HLSSVKY*SLQLYTLVFHQKQTTVSTHTAS 163
NLIWSRN+NTVIQLID*HLSSVK *SLQLY L FHQKQT V THTAS
Sbjct: 718 NLIWSRNKNTVIQLID*HLSSVKC*SLQLYALAFHQKQTIVLTHTAS 578
Score = 82.6 bits (174), Expect(2) = 3e-083
Identities = 37/45 (82%), Positives = 40/45 (88%)
Frame = +3 / -3
Query: 24 ILFGRGTKIQSSS*LTNT*AQ*SIDLSNYIHWSSIKNRRQCRLTQ 158
ILFG GTKIQS S*LTNT*AQ*S+DLSNY+HW SIKNRR C LT+
Sbjct: 717 ILFGPGTKIQSYS*LTNT*AQ*SVDLSNYMHWPSIKNRR*C*LTR 583
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 653,821,849
Number of Sequences: 832831
Number of extensions: 7824360
Number of successful extensions: 61075
Number of sequences better than 1.0e-020: 1
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 18545209515
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)