TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2455828.2.1
(1565 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF482743.1|CF482743 POL1_8_H02.g1_A002 Pollen Sorghum bi... 309 2e-083
gb|CD212492.1|CD212492 HS1_5_C09.g1_A012 Heat-shocked seedl... 294 6e-079
gb|BE360539.1|BE360539 DG1_64_E12.b1_A002 Dark Grown 1 (DG1... 256 1e-069
gb|CD212992.1|CD212992 HS1_17_D02.g1_A012 Heat-shocked seed... 246 5e-068
gb|BE360633.1|BE360633 DG1_64_E12.g1_A002 Dark Grown 1 (DG1... 214 3e-055
gb|CD212389.1|CD212389 HS1_5_C09.b1_A012 Heat-shocked seedl... 197 7e-050
gb|CF482661.1|CF482661 POL1_8_H02.b1_A002 Pollen Sorghum bi... 189 1e-048
gb|CD212886.1|CD212886 HS1_17_D02.b1_A012 Heat-shocked seed... 149 1e-043
gb|BG557893.1|BG557893 EM1_55_H09.b1_A002 Embryo 1 (EM1) So... 167 6e-042
gb|CD203946.1|CD203946 HS1_2_D07.g1_A012 Heat-shocked seedl... 150 6e-035
gb|CD203846.1|CD203846 HS1_2_D07.b1_A012 Heat-shocked seedl... 141 3e-032
gb|CW141260.1|CW141260 104_532_11136222_116_34935_029 Sorgh... 72 5e-021
>gb|CF482743.1|CF482743 POL1_8_H02.g1_A002 Pollen Sorghum bicolor cDNA clone POL1_8_H02_A002
5', mRNA sequence
Length = 707
Score = 309 bits (669), Expect(2) = 2e-083
Identities = 140/175 (80%), Positives = 144/175 (82%)
Frame = -3 / +1
Query: 1344 PPGALPSIRRAAPAGPSTGPGRWRQGTTTMCXXXXXXXXXXXSKRPIMGLQRSSIQTQIK 1165
PPG PS R AP G ST PGRWRQGTTTMC SKRPIMGLQRS IQT+IK
Sbjct: 178 PPGVRPSRHRVAPTGSSTEPGRWRQGTTTMCSASVRTLANQTSKRPIMGLQRSFIQTRIK 357
Query: 1164 VMRMQNASFKRFNELMRL*RMNKKDHYMIRLALISMRKLLLAEAELASRVVL*IHLRIFL 985
VM+MQN SFKRFNELMRL*RMNKKDHYMIRL LISMRKLLL EAELA RVVL IHLRIFL
Sbjct: 358 VMQMQNGSFKRFNELMRL*RMNKKDHYMIRLVLISMRKLLLVEAELALRVVLEIHLRIFL 537
Query: 984 VVAVAVVE*MISLGTFSGIGTLEDEMLRLHSNYHLWKQLKVAAKRSPSKRL*HVK 820
VVAVA VE*MISLGTFSGIGTLEDEMLRLH NYHLWKQ K AAKR +KRL*+VK
Sbjct: 538 VVAVAAVE*MISLGTFSGIGTLEDEMLRLHLNYHLWKQFKGAAKR*TTKRL*NVK 702
Score = 298 bits (645), Expect = 2e-079
Identities = 127/160 (79%), Positives = 133/160 (83%)
Frame = -2 / +3
Query: 1294 HGTRPVAARDYYDVLGVSKSASQAEIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 1115
HGTRPVAARDYYDVLGVSK+ASQ++IKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE
Sbjct: 228 HGTRPVAARDYYDVLGVSKNASQSDIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 407
Query: 1114 TLKDEQKRSLYDQVGPDQYEKTASXXXXXXXXXXFVNPFEDILXXXXXXXGMNDFFRNIL 935
TLKDEQKRSLYDQVGPDQYEK AS F NPFEDI GMNDFFRNI
Sbjct: 408 TLKDEQKRSLYDQVGPDQYEKAASGGGGTGFEGGFGNPFEDIFSGGGGSGGMNDFFRNIF 587
Query: 934 GDRDFGGRDVKVALELSSVEAAQGCSKTVTFQTSVTCEIC 815
DRDFGGRDVKVALELS +EA QGCSKT+ +QTSV CE C
Sbjct: 588 RDRDFGGRDVKVALELSFMEAVQGCSKTINYQTSVKCETC 707
Score = 236 bits (509), Expect(3) = 1e-081
Identities = 98/122 (80%), Positives = 102/122 (83%)
Frame = +1 / -3
Query: 988 EYPQMDLQNHPRSQFRLRQKQFSHTDQGQPDHIMIFFVHPLESHKLVEPLETCVLHPHHL 1167
+YPQMD QNHP+SQFRL QKQ SHTDQ QPDHIMIFFVHP +SHKLVEPLET VLH HHL
Sbjct: 534 KYPQMDFQNHPQSQFRLHQKQLSHTDQDQPDHIMIFFVHPSKSHKLVEPLETSVLHLHHL 355
Query: 1168 YLCLDGASLQAHNRPF*SPPGWRSC*RRAHRSSPLPPPAWSRGRTCGRCSADRRESSGRA 1347
Y CLD ASLQAHNRPF* G RS *RRAHRSSPLPPPAW RGRTCGR S RR +SGRA
Sbjct: 354 YSCLDEASLQAHNRPF*CLIG*RSY*RRAHRSSPLPPPAWFRGRTCGRYSVTRRANSGRA 175
Query: 1348 HR 1353
R
Sbjct: 174 PR 169
Score = 150 bits (323), Expect(2) = 3e-068
Identities = 66/78 (84%), Positives = 70/78 (89%)
Frame = +2 / -1
Query: 821 FTCYRRLEGDRFAATLSCFHR**FECNLNISSSKVPIPENVPKEIIHSTTATATTKNILK 1000
FT YRRL RFAA L+CFH+**F+CNLNISSSKVPIPENVPKEIIHST ATATTKNILK
Sbjct: 701 FTFYRRLVVYRFAAPLNCFHK**FKCNLNISSSKVPIPENVPKEIIHSTAATATTKNILK 522
Query: 1001 WIYKTTLEASSASARSSF 1054
WI KTTL+ASSAS RSSF
Sbjct: 521 WISKTTLKASSASTRSSF 468
Score = 142 bits (304), Expect(3) = 7e-067
Identities = 63/78 (80%), Positives = 67/78 (85%)
Frame = -1 / +2
Query: 1346 ALPELSLLSAEQRPQVLPRDQAGGGKGLLRCARRQQERQPGGDQKGLLWACKEAPSRHK* 1167
ALPE +LL E RPQVLPR+QAGGGKGLLRCARRQ ER P QKGLLWACKEA SRH+*
Sbjct: 176 ALPEFALLVTE*RPQVLPRNQAGGGKGLLRCARRQ*ER*PIRHQKGLLWACKEASSRHE* 355
Query: 1166 R*CGCRTQVSRGSTSL*D 1113
R*C CRT+VSRGSTSL*D
Sbjct: 356 R*CRCRTEVSRGSTSL*D 409
Score = 130 bits (279), Expect(2) = 3e-068
Identities = 62/86 (72%), Positives = 65/86 (75%)
Frame = +3 / -2
Query: 1044 EAVFSY*SGPT*SYNDLFCSSFRVS*AR*TS*NLRSASASPLFVSGWSFFASP**AFLIS 1223
EA FSY*SGPT*SYNDLFCSSF+VS*AR*TS*N RSASASPLFVSG SFFASP**AFL+S
Sbjct: 478 EAAFSY*SGPT*SYNDLFCSSFKVS*AR*TS*NFRSASASPLFVSG*SFFASP**AFLMS 299
Query: 1224 AWXXXXXXXXXXXXXXXXXGLVPWKD 1301
W GLVPWK+
Sbjct: 298 DWLAFLLTPSTS**SLAATGLVPWKN 221
Score = 123 bits (263), Expect(2) = 3e-040
Identities = 51/66 (77%), Positives = 53/66 (80%)
Frame = +2 / -1
Query: 1148 FCIRITFICVWMELLCKPIIGLFDLRLAGALADAEHIVVVPCRHRPGPVEGPAGAARRIE 1327
FCI ITFI VWM+LLCKPIIGLFD+ LA L DAEHIVVVPCRHRPG VE P GA R E
Sbjct: 374 FCICITFIRVWMKLLCKPIIGLFDV*LASVLTDAEHIVVVPCRHRPGSVEEPVGATR*RE 195
Query: 1328 GRAPGG 1345
GR PGG
Sbjct: 194 GRTPGG 177
Score = 94.1 bits (199), Expect(3) = 7e-067
Identities = 41/49 (83%), Positives = 43/49 (87%)
Frame = -1 / +2
Query: 962 NE*FL*EHSRG*GLWRTRC*GCTRTIICGSSSRLQQNGHLPNVCNM*NL 816
NE*FL*EH +G*GLWRTRC*GCT TII GSSSR+QQN LPNVC M*NL
Sbjct: 560 NE*FL*EHFQG*GLWRTRC*GCT*TIIYGSSSRVQQNDKLPNVCKM*NL 706
Score = 83.1 bits (175), Expect(3) = 1e-081
Identities = 34/43 (79%), Positives = 37/43 (86%)
Frame = +1 / -3
Query: 817 RFHMLQTFGR*PFCCNLELLPQMIVRVQP*HLVLQSPYPRECS 945
RFH+LQTFG FCC LELLP MIV+VQP*HLVLQSPYP +CS
Sbjct: 705 RFHILQTFGSLSFCCTLELLP*MIVQVQP*HLVLQSPYP*KCS 577
Score = 68.0 bits (142), Expect(2) = 3e-040
Identities = 32/64 (50%), Positives = 35/64 (54%)
Frame = +3 / -2
Query: 816 QISHVTDVWKVTVLLQPXXXXXXXXXXXXXXXRPPKSLSPRMFLKKSFIXXXXXXXXRIS 995
Q+SH TDVW VLL P RPPKSLS +MFLKKSFI +IS
Sbjct: 706 QVSHFTDVW*FIVLLHP*TASINDSSSATLTSRPPKSLSLKMFLKKSFIPPLPPPPLKIS 527
Query: 996 SNGF 1007
SNGF
Sbjct: 526 SNGF 515
Score = 59.7 bits (124), Expect(3) = 7e-067
Identities = 23/28 (82%), Positives = 24/28 (85%)
Frame = -1 / +2
Query: 1073 WP*SV*ENCFWRRRNWLRGWFCKSI*GY 990
W *SV*E+CFW RRNWL GWF KSI*GY
Sbjct: 449 WS*SV*ESCFWWRRNWL*GWFWKSI*GY 532
Score = 26.3 bits (51), Expect(3) = 1e-081
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +3 / -2
Query: 1410 ASRSKNRTPAWRRMAVA 1460
ASRS R PA RMAVA
Sbjct: 88 ASRSARRAPAGSRMAVA 38
Score = 23.1 bits (44), Expect(2) = 2e-083
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -1 / +2
Query: 1478 TTSQPPRHSHAPPRRGT 1428
T + RH HA PRR T
Sbjct: 20 TLAPVSRHRHAAPRRRT 70
>gb|CD212492.1|CD212492 HS1_5_C09.g1_A012 Heat-shocked seedlings Sorghum bicolor cDNA clone
HS1_5_C09_A012 5', mRNA sequence
Length = 692
Score = 294 bits (636), Expect(2) = 6e-079
Identities = 133/170 (78%), Positives = 137/170 (80%)
Frame = -3 / +1
Query: 1344 PPGALPSIRRAAPAGPSTGPGRWRQGTTTMCXXXXXXXXXXXSKRPIMGLQRSSIQTQIK 1165
PPG S R AP G ST PGRWRQGTTTMC SKRPIMGLQRS IQT+IK
Sbjct: 181 PPGVRASRHRVAPTGSSTEPGRWRQGTTTMCSASVRTLANQTSKRPIMGLQRSFIQTRIK 360
Query: 1164 VMRMQNASFKRFNELMRL*RMNKKDHYMIRLALISMRKLLLAEAELASRVVL*IHLRIFL 985
VM+MQN SFKRFNELMRL*RMNKKDHYMIRL LI+MRKLLL EAELA RVVL IHLRIFL
Sbjct: 361 VMQMQNGSFKRFNELMRL*RMNKKDHYMIRLVLINMRKLLLVEAELALRVVLEIHLRIFL 540
Query: 984 VVAVAVVE*MISLGTFSGIGTLEDEMLRLHSNYHLWKQLKVAAKRSPSKR 835
VVAVA VE*MISLGTFSGIGTL DEMLRLH NYHLWKQ K AAKR +KR
Sbjct: 541 VVAVAAVE*MISLGTFSGIGTLGDEMLRLHLNYHLWKQFKGAAKR*TTKR 690
Score = 286 bits (620), Expect = 4e-076
Identities = 123/154 (79%), Positives = 129/154 (83%)
Frame = -2 / +3
Query: 1294 HGTRPVAARDYYDVLGVSKSASQAEIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 1115
HGTRPVAARDYYDVLGVSK+ASQ++IKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE
Sbjct: 231 HGTRPVAARDYYDVLGVSKNASQSDIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 410
Query: 1114 TLKDEQKRSLYDQVGPDQYEKTASXXXXXXXXXXFVNPFEDILXXXXXXXGMNDFFRNIL 935
TLKDEQKRSLYDQVGPDQYEK AS F NPFEDI GMNDFFRNI
Sbjct: 411 TLKDEQKRSLYDQVGPDQYEKAASGGGGTGFEGGFGNPFEDIFSGGGGSGGMNDFFRNIF 590
Query: 934 GDRDFGGRDVKVALELSSVEAAQGCSKTVTFQTS 833
DRDFGGRDVKVALELS +EA QGCSKT+ +QTS
Sbjct: 591 RDRDFGGRDVKVALELSFMEAVQGCSKTINYQTS 692
Score = 230 bits (497), Expect(3) = 4e-075
Identities = 96/122 (78%), Positives = 100/122 (81%)
Frame = +1 / -3
Query: 988 EYPQMDLQNHPRSQFRLRQKQFSHTDQGQPDHIMIFFVHPLESHKLVEPLETCVLHPHHL 1167
+YPQMD QNHP+SQFRL QKQ SH DQ QPDHIMIFFVHP +SHKLVEPLET VLH HHL
Sbjct: 537 KYPQMDFQNHPQSQFRLHQKQLSHIDQDQPDHIMIFFVHPSKSHKLVEPLETSVLHLHHL 358
Query: 1168 YLCLDGASLQAHNRPF*SPPGWRSC*RRAHRSSPLPPPAWSRGRTCGRCSADRRESSGRA 1347
Y CLD ASLQAHNRPF* G RS *RRAHRSSPLPPPAW RGRTCGR S R +SGRA
Sbjct: 357 YSCLDEASLQAHNRPF*CLIG*RSY*RRAHRSSPLPPPAWFRGRTCGRYSVTRSANSGRA 178
Query: 1348 HR 1353
R
Sbjct: 177 PR 172
Score = 142 bits (304), Expect(3) = 2e-062
Identities = 63/78 (80%), Positives = 67/78 (85%)
Frame = -1 / +2
Query: 1346 ALPELSLLSAEQRPQVLPRDQAGGGKGLLRCARRQQERQPGGDQKGLLWACKEAPSRHK* 1167
ALPE +LL E RPQVLPR+QAGGGKGLLRCARRQ ER P QKGLLWACKEA SRH+*
Sbjct: 179 ALPEFALLVTE*RPQVLPRNQAGGGKGLLRCARRQ*ER*PIRHQKGLLWACKEASSRHE* 358
Query: 1166 R*CGCRTQVSRGSTSL*D 1113
R*C CRT+VSRGSTSL*D
Sbjct: 359 R*CRCRTEVSRGSTSL*D 412
Score = 141 bits (302), Expect(2) = 2e-065
Identities = 62/74 (83%), Positives = 66/74 (89%)
Frame = +2 / -1
Query: 833 RRLEGDRFAATLSCFHR**FECNLNISSSKVPIPENVPKEIIHSTTATATTKNILKWIYK 1012
RRL RFAA L+CFH+**F+CNLNISS KVPIPENVPKEIIHST ATATTKNILKWI K
Sbjct: 692 RRLVVYRFAAPLNCFHK**FKCNLNISSPKVPIPENVPKEIIHSTAATATTKNILKWISK 513
Query: 1013 TTLEASSASARSSF 1054
TTL+ASSAS RSSF
Sbjct: 512 TTLKASSASTRSSF 471
Score = 130 bits (279), Expect(2) = 2e-065
Identities = 62/86 (72%), Positives = 65/86 (75%)
Frame = +3 / -2
Query: 1044 EAVFSY*SGPT*SYNDLFCSSFRVS*AR*TS*NLRSASASPLFVSGWSFFASP**AFLIS 1223
EA FSY*SGPT*SYNDLFCSSF+VS*AR*TS*N RSASASPLFVSG SFFASP**AFL+S
Sbjct: 481 EAAFSY*SGPT*SYNDLFCSSFKVS*AR*TS*NFRSASASPLFVSG*SFFASP**AFLMS 302
Query: 1224 AWXXXXXXXXXXXXXXXXXGLVPWKD 1301
W GLVPWK+
Sbjct: 301 DWLAFLLTPSTS**SLAATGLVPWKN 224
Score = 120 bits (257), Expect(2) = 4e-036
Identities = 50/66 (75%), Positives = 52/66 (78%)
Frame = +2 / -1
Query: 1148 FCIRITFICVWMELLCKPIIGLFDLRLAGALADAEHIVVVPCRHRPGPVEGPAGAARRIE 1327
FCI ITFI VWM+LLCKPIIGLFD+ LA L DAEHIVVVPCRHRPG VE P GA R E
Sbjct: 377 FCICITFIRVWMKLLCKPIIGLFDV*LASVLTDAEHIVVVPCRHRPGSVEEPVGATR*RE 198
Query: 1328 GRAPGG 1345
R PGG
Sbjct: 197 ARTPGG 180
Score = 79.4 bits (167), Expect(3) = 2e-062
Identities = 35/43 (81%), Positives = 37/43 (86%)
Frame = -1 / +2
Query: 962 NE*FL*EHSRG*GLWRTRC*GCTRTIICGSSSRLQQNGHLPNV 834
NE*FL*EH +G*GLW TRC*GCT TII GSSSR+QQN LPNV
Sbjct: 563 NE*FL*EHFQG*GLWGTRC*GCT*TIIYGSSSRVQQNDKLPNV 691
Score = 66.6 bits (139), Expect(3) = 4e-075
Identities = 28/37 (75%), Positives = 30/37 (81%)
Frame = +1 / -3
Query: 835 TFGR*PFCCNLELLPQMIVRVQP*HLVLQSPYPRECS 945
TFG FCC LELLP MIV+VQP*HLV QSPYP +CS
Sbjct: 690 TFGSLSFCCTLELLP*MIVQVQP*HLVPQSPYP*KCS 580
Score = 59.3 bits (123), Expect(3) = 2e-062
Identities = 22/28 (78%), Positives = 24/28 (85%)
Frame = -1 / +2
Query: 1073 WP*SV*ENCFWRRRNWLRGWFCKSI*GY 990
W *S+*E+CFW RRNWL GWF KSI*GY
Sbjct: 452 WS*SI*ESCFWWRRNWL*GWFWKSI*GY 535
Score = 57.0 bits (118), Expect(2) = 4e-036
Identities = 28/58 (48%), Positives = 30/58 (51%)
Frame = +3 / -2
Query: 834 DVWKVTVLLQPXXXXXXXXXXXXXXXRPPKSLSPRMFLKKSFIXXXXXXXXRISSNGF 1007
DVW VLL P RPPKSLS +MFLKKSFI +ISSNGF
Sbjct: 691 DVW*FIVLLHP*TASINDSSSATLTSRPPKSLSLKMFLKKSFIPPLPPPPLKISSNGF 518
Score = 26.3 bits (51), Expect(3) = 4e-075
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +3 / -2
Query: 1410 ASRSKNRTPAWRRMAVA 1460
ASRS R PA RMAVA
Sbjct: 91 ASRSARRAPAGSRMAVA 41
Score = 23.1 bits (44), Expect(2) = 6e-079
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -1 / +2
Query: 1478 TTSQPPRHSHAPPRRGT 1428
T + RH HA PRR T
Sbjct: 23 TRAPVSRHRHAAPRRRT 73
>gb|BE360539.1|BE360539 DG1_64_E12.b1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 601
Score = 256 bits (553), Expect(2) = 2e-067
Identities = 116/144 (80%), Positives = 118/144 (81%)
Frame = -3 / +2
Query: 1344 PPGALPSIRRAAPAGPSTGPGRWRQGTTTMCXXXXXXXXXXXSKRPIMGLQRSSIQTQIK 1165
PPG PS R AP G ST PGRWRQGTTTMC SKRPIMGLQRS IQT+IK
Sbjct: 170 PPGVRPSRHRVAPTGSSTEPGRWRQGTTTMCSASVRTLANQTSKRPIMGLQRSFIQTRIK 349
Query: 1164 VMRMQNASFKRFNELMRL*RMNKKDHYMIRLALISMRKLLLAEAELASRVVL*IHLRIFL 985
VM+MQN SFKRFNELMRL*RMNKKDHYMIRL LISMRKLLL EAELA RVVL IHLRIFL
Sbjct: 350 VMQMQNGSFKRFNELMRL*RMNKKDHYMIRLVLISMRKLLLVEAELALRVVLEIHLRIFL 529
Query: 984 VVAVAVVE*MISLGTFSGIGTLED 913
VVAVA VE*MISLGTFSGIGTLED
Sbjct: 530 VVAVAAVE*MISLGTFSGIGTLED 601
Score = 237 bits (513), Expect = 3e-061
Identities = 102/127 (80%), Positives = 105/127 (82%)
Frame = -2 / +1
Query: 1294 HGTRPVAARDYYDVLGVSKSASQAEIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 1115
HGTRPVAARDYYDVLGVSK+ASQ++IKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE
Sbjct: 220 HGTRPVAARDYYDVLGVSKNASQSDIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 399
Query: 1114 TLKDEQKRSLYDQVGPDQYEKTASXXXXXXXXXXFVNPFEDILXXXXXXXGMNDFFRNIL 935
TLKDEQKRSLYDQVGPDQYEK AS F NPFEDI GMNDFFRNI
Sbjct: 400 TLKDEQKRSLYDQVGPDQYEKAASGGGGTGFEGGFGNPFEDIFSGGGGSGGMNDFFRNIF 579
Query: 934 GDRDFGG 914
DRDFGG
Sbjct: 580 RDRDFGG 600
Score = 236 bits (509), Expect(3) = 1e-069
Identities = 98/122 (80%), Positives = 102/122 (83%)
Frame = +1 / -1
Query: 988 EYPQMDLQNHPRSQFRLRQKQFSHTDQGQPDHIMIFFVHPLESHKLVEPLETCVLHPHHL 1167
+YPQMD QNHP+SQFRL QKQ SHTDQ QPDHIMIFFVHP +SHKLVEPLET VLH HHL
Sbjct: 526 KYPQMDFQNHPQSQFRLHQKQLSHTDQDQPDHIMIFFVHPSKSHKLVEPLETSVLHLHHL 347
Query: 1168 YLCLDGASLQAHNRPF*SPPGWRSC*RRAHRSSPLPPPAWSRGRTCGRCSADRRESSGRA 1347
Y CLD ASLQAHNRPF* G RS *RRAHRSSPLPPPAW RGRTCGR S RR +SGRA
Sbjct: 346 YSCLDEASLQAHNRPF*CLIG*RSY*RRAHRSSPLPPPAWFRGRTCGRYSVTRRANSGRA 167
Query: 1348 HR 1353
R
Sbjct: 166 PR 161
Score = 142 bits (304), Expect(3) = 5e-049
Identities = 63/78 (80%), Positives = 67/78 (85%)
Frame = -1 / +3
Query: 1346 ALPELSLLSAEQRPQVLPRDQAGGGKGLLRCARRQQERQPGGDQKGLLWACKEAPSRHK* 1167
ALPE +LL E RPQVLPR+QAGGGKGLLRCARRQ ER P QKGLLWACKEA SRH+*
Sbjct: 168 ALPEFALLVTE*RPQVLPRNQAGGGKGLLRCARRQ*ER*PIRHQKGLLWACKEASSRHE* 347
Query: 1166 R*CGCRTQVSRGSTSL*D 1113
R*C CRT+VSRGSTSL*D
Sbjct: 348 R*CRCRTEVSRGSTSL*D 401
Score = 130 bits (279), Expect(2) = 7e-053
Identities = 62/86 (72%), Positives = 65/86 (75%)
Frame = +3 / -3
Query: 1044 EAVFSY*SGPT*SYNDLFCSSFRVS*AR*TS*NLRSASASPLFVSGWSFFASP**AFLIS 1223
EA FSY*SGPT*SYNDLFCSSF+VS*AR*TS*N RSASASPLFVSG SFFASP**AFL+S
Sbjct: 470 EAAFSY*SGPT*SYNDLFCSSFKVS*AR*TS*NFRSASASPLFVSG*SFFASP**AFLMS 291
Query: 1224 AWXXXXXXXXXXXXXXXXXGLVPWKD 1301
W GLVPWK+
Sbjct: 290 DWLAFLLTPSTS**SLAATGLVPWKN 213
Score = 123 bits (263), Expect(2) = 2e-027
Identities = 51/66 (77%), Positives = 53/66 (80%)
Frame = +2 / -2
Query: 1148 FCIRITFICVWMELLCKPIIGLFDLRLAGALADAEHIVVVPCRHRPGPVEGPAGAARRIE 1327
FCI ITFI VWM+LLCKPIIGLFD+ LA L DAEHIVVVPCRHRPG VE P GA R E
Sbjct: 366 FCICITFIRVWMKLLCKPIIGLFDV*LASVLTDAEHIVVVPCRHRPGSVEEPVGATR*RE 187
Query: 1328 GRAPGG 1345
GR PGG
Sbjct: 186 GRTPGG 169
Score = 99.6 bits (211), Expect(2) = 7e-053
Identities = 43/47 (91%), Positives = 44/47 (93%)
Frame = +2 / -2
Query: 914 SSKVPIPENVPKEIIHSTTATATTKNILKWIYKTTLEASSASARSSF 1054
SSKVPIPENVPKEIIHST ATATTKNILKWI KTTL+ASSAS RSSF
Sbjct: 600 SSKVPIPENVPKEIIHSTAATATTKNILKWISKTTLKASSASTRSSF 460
Score = 59.7 bits (124), Expect(3) = 5e-049
Identities = 23/28 (82%), Positives = 24/28 (85%)
Frame = -1 / +3
Query: 1073 WP*SV*ENCFWRRRNWLRGWFCKSI*GY 990
W *SV*E+CFW RRNWL GWF KSI*GY
Sbjct: 441 WS*SV*ESCFWWRRNWL*GWFWKSI*GY 524
Score = 42.8 bits (87), Expect(3) = 1e-069
Identities = 20/31 (64%), Positives = 22/31 (70%)
Frame = +3 / -3
Query: 915 PPKSLSPRMFLKKSFIXXXXXXXXRISSNGF 1007
PPKSLS +MFLKKSFI +ISSNGF
Sbjct: 599 PPKSLSLKMFLKKSFIPPLPPPPLKISSNGF 507
Score = 34.1 bits (68), Expect(3) = 5e-049
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = -1 / +3
Query: 962 NE*FL*EHSRG*GLWR 915
NE*FL*EH +G*GLWR
Sbjct: 552 NE*FL*EHFQG*GLWR 599
Score = 26.3 bits (51), Expect(3) = 1e-069
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +3 / -3
Query: 1410 ASRSKNRTPAWRRMAVA 1460
ASRS R PA RMAVA
Sbjct: 80 ASRSARRAPAGSRMAVA 30
Score = 24.9 bits (48), Expect(2) = 2e-027
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = +1 / -1
Query: 913 VLQSPYPRECS 945
VLQSPYP +CS
Sbjct: 601 VLQSPYP*KCS 569
Score = 23.1 bits (44), Expect(2) = 2e-067
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -1 / +3
Query: 1478 TTSQPPRHSHAPPRRGT 1428
T + RH HA PRR T
Sbjct: 12 TRAPVSRHRHAAPRRRT 62
>gb|CD212992.1|CD212992 HS1_17_D02.g1_A012 Heat-shocked seedlings Sorghum bicolor cDNA clone
HS1_17_D02_A012 5', mRNA sequence
Length = 587
Score = 246 bits (531), Expect(2) = 2e-064
Identities = 112/142 (78%), Positives = 115/142 (80%)
Frame = -3 / +1
Query: 1344 PPGALPSIRRAAPAGPSTGPGRWRQGTTTMCXXXXXXXXXXXSKRPIMGLQRSSIQTQIK 1165
PPG S R AP G ST PGRWRQGTTTMC SKRPIMGLQRS IQT+IK
Sbjct: 157 PPGVRASRHRVAPTGSSTEPGRWRQGTTTMCSASVRTLANQTSKRPIMGLQRSFIQTRIK 336
Query: 1164 VMRMQNASFKRFNELMRL*RMNKKDHYMIRLALISMRKLLLAEAELASRVVL*IHLRIFL 985
VM+MQN SFKRFNELMRL*RMNKKDHYMIRL LI+MRKLLL EAELA RVVL IHLRIFL
Sbjct: 337 VMQMQNGSFKRFNELMRL*RMNKKDHYMIRLVLINMRKLLLVEAELALRVVLEIHLRIFL 516
Query: 984 VVAVAVVE*MISLGTFSGIGTL 919
VVAVA VE*MISLGTFSGIGTL
Sbjct: 517 VVAVAAVE*MISLGTFSGIGTL 582
Score = 237 bits (513), Expect = 3e-061
Identities = 102/127 (80%), Positives = 105/127 (82%)
Frame = -2 / +3
Query: 1294 HGTRPVAARDYYDVLGVSKSASQAEIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 1115
HGTRPVAARDYYDVLGVSK+ASQ++IKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE
Sbjct: 207 HGTRPVAARDYYDVLGVSKNASQSDIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 386
Query: 1114 TLKDEQKRSLYDQVGPDQYEKTASXXXXXXXXXXFVNPFEDILXXXXXXXGMNDFFRNIL 935
TLKDEQKRSLYDQVGPDQYEK AS F NPFEDI GMNDFFRNI
Sbjct: 387 TLKDEQKRSLYDQVGPDQYEKAASGGGGTGFEGGFGNPFEDIFSGGGGSGGMNDFFRNIF 566
Query: 934 GDRDFGG 914
DRDFGG
Sbjct: 567 RDRDFGG 587
Score = 230 bits (497), Expect(3) = 5e-068
Identities = 96/122 (78%), Positives = 100/122 (81%)
Frame = +1 / -3
Query: 988 EYPQMDLQNHPRSQFRLRQKQFSHTDQGQPDHIMIFFVHPLESHKLVEPLETCVLHPHHL 1167
+YPQMD QNHP+SQFRL QKQ SH DQ QPDHIMIFFVHP +SHKLVEPLET VLH HHL
Sbjct: 513 KYPQMDFQNHPQSQFRLHQKQLSHIDQDQPDHIMIFFVHPSKSHKLVEPLETSVLHLHHL 334
Query: 1168 YLCLDGASLQAHNRPF*SPPGWRSC*RRAHRSSPLPPPAWSRGRTCGRCSADRRESSGRA 1347
Y CLD ASLQAHNRPF* G RS *RRAHRSSPLPPPAW RGRTCGR S R +SGRA
Sbjct: 333 YSCLDEASLQAHNRPF*CLIG*RSY*RRAHRSSPLPPPAWFRGRTCGRYSVTRSANSGRA 154
Query: 1348 HR 1353
R
Sbjct: 153 PR 148
Score = 142 bits (304), Expect(3) = 3e-048
Identities = 63/78 (80%), Positives = 67/78 (85%)
Frame = -1 / +2
Query: 1346 ALPELSLLSAEQRPQVLPRDQAGGGKGLLRCARRQQERQPGGDQKGLLWACKEAPSRHK* 1167
ALPE +LL E RPQVLPR+QAGGGKGLLRCARRQ ER P QKGLLWACKEA SRH+*
Sbjct: 155 ALPEFALLVTE*RPQVLPRNQAGGGKGLLRCARRQ*ER*PIRHQKGLLWACKEASSRHE* 334
Query: 1166 R*CGCRTQVSRGSTSL*D 1113
R*C CRT+VSRGSTSL*D
Sbjct: 335 R*CRCRTEVSRGSTSL*D 388
Score = 130 bits (279), Expect(2) = 3e-052
Identities = 62/86 (72%), Positives = 65/86 (75%)
Frame = +3 / -2
Query: 1044 EAVFSY*SGPT*SYNDLFCSSFRVS*AR*TS*NLRSASASPLFVSGWSFFASP**AFLIS 1223
EA FSY*SGPT*SYNDLFCSSF+VS*AR*TS*N RSASASPLFVSG SFFASP**AFL+S
Sbjct: 457 EAAFSY*SGPT*SYNDLFCSSFKVS*AR*TS*NFRSASASPLFVSG*SFFASP**AFLMS 278
Query: 1224 AWXXXXXXXXXXXXXXXXXGLVPWKD 1301
W GLVPWK+
Sbjct: 277 DWLAFLLTPSTS**SLAATGLVPWKN 200
Score = 120 bits (257), Expect = 5e-026
Identities = 50/66 (75%), Positives = 52/66 (78%)
Frame = +2 / -1
Query: 1148 FCIRITFICVWMELLCKPIIGLFDLRLAGALADAEHIVVVPCRHRPGPVEGPAGAARRIE 1327
FCI ITFI VWM+LLCKPIIGLFD+ LA L DAEHIVVVPCRHRPG VE P GA R E
Sbjct: 353 FCICITFIRVWMKLLCKPIIGLFDV*LASVLTDAEHIVVVPCRHRPGSVEEPVGATR*RE 174
Query: 1328 GRAPGG 1345
R PGG
Sbjct: 173 ARTPGG 156
Score = 97.3 bits (206), Expect(2) = 3e-052
Identities = 42/47 (89%), Positives = 43/47 (91%)
Frame = +2 / -1
Query: 914 SSKVPIPENVPKEIIHSTTATATTKNILKWIYKTTLEASSASARSSF 1054
S KVPIPENVPKEIIHST ATATTKNILKWI KTTL+ASSAS RSSF
Sbjct: 587 SPKVPIPENVPKEIIHSTAATATTKNILKWISKTTLKASSASTRSSF 447
Score = 59.3 bits (123), Expect(3) = 3e-048
Identities = 22/28 (78%), Positives = 24/28 (85%)
Frame = -1 / +2
Query: 1073 WP*SV*ENCFWRRRNWLRGWFCKSI*GY 990
W *S+*E+CFW RRNWL GWF KSI*GY
Sbjct: 428 WS*SI*ESCFWWRRNWL*GWFWKSI*GY 511
Score = 42.8 bits (87), Expect(3) = 5e-068
Identities = 20/31 (64%), Positives = 22/31 (70%)
Frame = +3 / -2
Query: 915 PPKSLSPRMFLKKSFIXXXXXXXXRISSNGF 1007
PPKSLS +MFLKKSFI +ISSNGF
Sbjct: 586 PPKSLSLKMFLKKSFIPPLPPPPLKISSNGF 494
Score = 31.8 bits (63), Expect(3) = 3e-048
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = -1 / +2
Query: 962 NE*FL*EHSRG*GLW 918
NE*FL*EH +G*GLW
Sbjct: 539 NE*FL*EHFQG*GLW 583
Score = 26.3 bits (51), Expect(3) = 5e-068
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +3 / -2
Query: 1410 ASRSKNRTPAWRRMAVA 1460
ASRS R PA RMAVA
Sbjct: 67 ASRSARRAPAGSRMAVA 17
Score = 22.6 bits (43), Expect(2) = 2e-064
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = -1 / +2
Query: 1460 RHSHAPPRRGT 1428
RH HA PRR T
Sbjct: 17 RHRHAAPRRRT 49
>gb|BE360633.1|BE360633 DG1_64_E12.g1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 578
Score = 214 bits (461), Expect(2) = 3e-055
Identities = 89/98 (90%), Positives = 93/98 (94%)
Frame = -2 / +2
Query: 520 TLEVREDPVFRREKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVLKIKPGTQPGQRVVLR 341
TL+VREDPVFRREKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVLK+KPGTQPGQ+VVLR
Sbjct: 2 TLKVREDPVFRREKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVLKVKPGTQPGQKVVLR 181
Query: 340 GKGIKTRNSPYHGDQYVHFNVNIPENLTSRQRELIEEF 227
GKGIK RNSPY+GDQYVHFNV IP NLT RQR LIEEF
Sbjct: 182 GKGIKARNSPYYGDQYVHFNVTIPMNLTPRQRVLIEEF 295
Score = 182 bits (393), Expect = 9e-045
Identities = 73/91 (80%), Positives = 77/91 (84%)
Frame = -1 / +1
Query: 521 DPRGS*GSCFPKRER*YSC*CCPECDSGNFGRDSSSPNSQWRRCSKDQARYSTWSEGSSE 342
DP+GS*GSC P R+R*YSC*CC ECDSGNFGRDSSSPNS WRRCSK Q YSTWSEGSSE
Sbjct: 1 DPQGS*GSCIP*RKR*YSC*CCSECDSGNFGRDSSSPNS*WRRCSKGQTWYSTWSEGSSE 180
Query: 341 RKRDQDKKLTVPRRSICPFQCQHPRKFDV*T 249
RKRDQ KKLT+ RSICPFQC HP +FD T
Sbjct: 181 RKRDQGKKLTILWRSICPFQCHHPYEFDTKT 273
Score = 170 bits (365), Expect(2) = 1e-041
Identities = 74/87 (85%), Positives = 78/87 (89%)
Frame = +2 / -2
Query: 260 QIFGDVDIEMDILISVVR*VSCLDPFSSQNYPLTRLSTGLDL*NNVSTESWDLNCPSQNC 439
QI D DIEMDILIS+V *VSCLDPFSSQNY LTRLST DL*NNVST+SWDLNCPSQNC
Sbjct: 262 QIHRDGDIEMDILISIVW*VSCLDPFSSQNYLLTRLSTRFDL*NNVSTKSWDLNCPSQNC 83
Query: 440 LSHIQDSINMNITFLSSENRILTNLEG 520
LSHIQ+SINMNITF S+E RILTNLEG
Sbjct: 82 LSHIQNSINMNITFFSTEYRILTNLEG 2
Score = 169 bits (364), Expect(2) = 2e-041
Identities = 75/82 (91%), Positives = 76/82 (92%)
Frame = +3 / -3
Query: 246 RCLDVKFSGMLTLKWTY*SPWYGEFLVLIPFPLRTTL*PG*VPGLIFRTTSPLRVGT*TV 425
RCL VKF GM+TLKWTY*SP YGEFL LIPFPLRTT *PG*VPGL FRTTSPLRVGT*TV
Sbjct: 276 RCLGVKFIGMVTLKWTY*SP*YGEFLALIPFPLRTTF*PG*VPGLTFRTTSPLRVGT*TV 97
Query: 426 PPKIA*VTFRTAST*ISPFSLR 491
PPKIA*VTFRTAST*ISPFSLR
Sbjct: 96 PPKIA*VTFRTAST*ISPFSLR 31
Score = 153 bits (328), Expect = 8e-036
Identities = 72/83 (86%), Positives = 75/83 (90%)
Frame = -3 / +3
Query: 519 PSRFVRILFSEERKVIFMLMLS*M*LRQFWEGQFKSQLSVETLF*RSSPVLNLVRG*F*E 340
PSRFVRIL+S E+KVIFMLML *M*LRQFWEGQFKSQL VETLF*RS+ VLNLVR *F*E
Sbjct: 3 PSRFVRILYSVEKKVIFMLMLF*M*LRQFWEGQFKSQLLVETLF*RSNLVLNLVRR*F*E 182
Query: 339 EKGSRQETHRTTEINMSISMSTS 271
EKGSRQETH T EINMSISMS S
Sbjct: 183 EKGSRQETHHTMEINMSISMSPS 251
Score = 135 bits (289), Expect = 2e-030
Identities = 61/87 (70%), Positives = 63/87 (72%)
Frame = +1 / -1
Query: 259 SNFRGC*H*NGHIDLRGTVXXXXXXXXXXXXPSDQVEYRA*SLEQRLH*ELGLELSLPKL 438
SN G *H*NGHIDL V PSDQVEY+ * LEQRLH*ELGLELSLPKL
Sbjct: 263 SNS*GW*H*NGHIDLHSMVSFLP*SLFLSELPSDQVEYQV*PLEQRLH*ELGLELSLPKL 84
Query: 439 PESHSGQHQHEYHLSLFGKQDPHEPRG 519
PESHS QHQHEYHL L+G QDPHEP G
Sbjct: 83 PESHSEQHQHEYHLFLYGIQDPHEP*G 3
Score = 27.2 bits (53), Expect(2) = 3e-055
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = -2 / +1
Query: 70 IGLCFGFCSWN 38
I LC GFC WN
Sbjct: 493 IDLCLGFCRWN 525
Score = 25.8 bits (50), Expect(2) = 1e-041
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = +3 / -1
Query: 36 LFQLQNPKQRPI 71
LF LQNPKQR I
Sbjct: 527 LFHLQNPKQRSI 492
Score = 25.4 bits (49), Expect(2) = 2e-041
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = +1 / -2
Query: 28 LFIYSSYKIRNKDLSI 75
++ YS YKI NKDL +
Sbjct: 535 IYYYSIYKIPNKDLYV 488
>gb|CD212389.1|CD212389 HS1_5_C09.b1_A012 Heat-shocked seedlings Sorghum bicolor cDNA clone
HS1_5_C09_A012 3', mRNA sequence
Length = 657
Score = 197 bits (424), Expect(2) = 7e-050
Identities = 82/90 (91%), Positives = 85/90 (94%)
Frame = -2 / +2
Query: 496 VFRREKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVLKIKPGTQPGQRVVLRGKGIKTRN 317
VFRREKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVLK+KPGTQPGQ+VVLRGKGIK RN
Sbjct: 2 VFRREKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVLKVKPGTQPGQKVVLRGKGIKARN 181
Query: 316 SPYHGDQYVHFNVNIPENLTSRQRELIEEF 227
SPY+GDQYVHFNV IP NLT RQR LIEEF
Sbjct: 182 SPYYGDQYVHFNVTIPVNLTPRQRVLIEEF 271
Score = 171 bits (367), Expect(2) = 6e-042
Identities = 75/82 (91%), Positives = 77/82 (93%)
Frame = +3 / -1
Query: 246 RCLDVKFSGMLTLKWTY*SPWYGEFLVLIPFPLRTTL*PG*VPGLIFRTTSPLRVGT*TV 425
RCL VKF+GM+TLKWTY*SP YGEFL LIPFPLRTT *PG*VPGL FRTTSPLRVGT*TV
Sbjct: 252 RCLGVKFTGMVTLKWTY*SP*YGEFLALIPFPLRTTF*PG*VPGLTFRTTSPLRVGT*TV 73
Query: 426 PPKIA*VTFRTAST*ISPFSLR 491
PPKIA*VTFRTAST*ISPFSLR
Sbjct: 72 PPKIA*VTFRTAST*ISPFSLR 7
Score = 162 bits (349), Expect = 1e-038
Identities = 66/83 (79%), Positives = 68/83 (81%)
Frame = -1 / +1
Query: 497 CFPKRER*YSC*CCPECDSGNFGRDSSSPNSQWRRCSKDQARYSTWSEGSSERKRDQDKK 318
C P RER*YSC*CC ECDSGNFGRDSSSPNS WRRCSK Q YSTWSEGSSERKRDQ KK
Sbjct: 1 CIP*RER*YSC*CCSECDSGNFGRDSSSPNS*WRRCSKGQTWYSTWSEGSSERKRDQGKK 180
Query: 317 LTVPRRSICPFQCQHPRKFDV*T 249
LT+ RSICPFQ HP +FD T
Sbjct: 181 LTILWRSICPFQRHHPCEFDTKT 249
Score = 154 bits (331), Expect(2) = 7e-037
Identities = 66/78 (84%), Positives = 71/78 (91%)
Frame = +2 / -3
Query: 260 QIFGDVDIEMDILISVVR*VSCLDPFSSQNYPLTRLSTGLDL*NNVSTESWDLNCPSQNC 439
QI D D+EMDILIS+V *VSCLDPFSSQNY LTRLST DL*NNVST+SWDLNCPSQNC
Sbjct: 238 QIHRDGDVEMDILISIVW*VSCLDPFSSQNYLLTRLSTRFDL*NNVSTKSWDLNCPSQNC 59
Query: 440 LSHIQDSINMNITFLSSE 493
LSHIQ+SINMNITFLS+E
Sbjct: 58 LSHIQNSINMNITFLSTE 5
Score = 133 bits (286), Expect = 5e-030
Identities = 64/75 (85%), Positives = 66/75 (88%)
Frame = -3 / +3
Query: 495 FSEERKVIFMLMLS*M*LRQFWEGQFKSQLSVETLF*RSSPVLNLVRG*F*EEKGSRQET 316
+S ERKVIFMLML *M*LRQFWEGQFKSQL VETLF*RS+ VLNLVR *F*EEKGSRQET
Sbjct: 3 YSVERKVIFMLMLF*M*LRQFWEGQFKSQLLVETLF*RSNLVLNLVRR*F*EEKGSRQET 182
Query: 315 HRTTEINMSISMSTS 271
H T EINMSIS S S
Sbjct: 183 HHTMEINMSISTSPS 227
Score = 119 bits (255), Expect = 1e-025
Identities = 54/78 (69%), Positives = 57/78 (73%)
Frame = +1 / -2
Query: 259 SNFRGC*H*NGHIDLRGTVXXXXXXXXXXXXPSDQVEYRA*SLEQRLH*ELGLELSLPKL 438
SN +G * *NGHIDL V PSDQVEY+ * LEQRLH*ELGLELSLPKL
Sbjct: 239 SNSQGW*R*NGHIDLHSMVSFLP*SLFLSELPSDQVEYQV*PLEQRLH*ELGLELSLPKL 60
Query: 439 PESHSGQHQHEYHLSLFG 492
PESHS QHQHEYHLSL+G
Sbjct: 59 PESHSEQHQHEYHLSLYG 6
Score = 26.3 bits (51), Expect(2) = 7e-050
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = -2 / +2
Query: 70 IGLCFGFCSWN 38
I LC G CSWN
Sbjct: 470 IDLCLGVCSWN 502
Score = 25.8 bits (50), Expect(2) = 6e-042
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / -1
Query: 12 ISSRRIIHLFQLQNPKQRPI 71
IS + LFQLQ PKQR I
Sbjct: 528 ISGTGDLVLFQLQTPKQRSI 469
Score = 25.4 bits (49), Expect(2) = 7e-037
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +1 / -2
Query: 37 YSSYKIRNKDLSI 75
YSSYK+ NKDL +
Sbjct: 503 YSSYKLPNKDLYV 465
>gb|CF482661.1|CF482661 POL1_8_H02.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_8_H02_A002 3', mRNA sequence
Length = 648
Score = 189 bits (408), Expect(2) = 1e-048
Identities = 78/87 (89%), Positives = 82/87 (94%)
Frame = -2 / +2
Query: 487 REKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVLKIKPGTQPGQRVVLRGKGIKTRNSPY 308
REKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVLK+KPGTQPGQ+VVLRGKGIK RNSPY
Sbjct: 2 REKGDIHVDAVLNVTQAILGGTVQVPTLSGDVVLKVKPGTQPGQKVVLRGKGIKARNSPY 181
Query: 307 HGDQYVHFNVNIPENLTSRQRELIEEF 227
+GDQYVHFNV +P NLT RQR LIEEF
Sbjct: 182 YGDQYVHFNVTVPMNLTPRQRVLIEEF 262
Score = 164 bits (353), Expect(2) = 8e-041
Identities = 73/81 (90%), Positives = 74/81 (91%)
Frame = +3 / -1
Query: 246 RCLDVKFSGMLTLKWTY*SPWYGEFLVLIPFPLRTTL*PG*VPGLIFRTTSPLRVGT*TV 425
RCL VKF G +TLKWTY*SP YGEFL LIPFPLRTT *PG*VPGL FRTTSPLRVGT*TV
Sbjct: 243 RCLGVKFIGTVTLKWTY*SP*YGEFLALIPFPLRTTF*PG*VPGLTFRTTSPLRVGT*TV 64
Query: 426 PPKIA*VTFRTAST*ISPFSL 488
PPKIA*VTFRTAST*ISPFSL
Sbjct: 63 PPKIA*VTFRTAST*ISPFSL 1
Score = 157 bits (338), Expect = 3e-037
Identities = 63/79 (79%), Positives = 66/79 (83%)
Frame = -1 / +1
Query: 485 RER*YSC*CCPECDSGNFGRDSSSPNSQWRRCSKDQARYSTWSEGSSERKRDQDKKLTVP 306
R+R*YSC*CC ECDSGNFGRDSSSPNS WRRCSK Q YSTWSEGSSERKRDQ KKLT+
Sbjct: 4 RKR*YSC*CCSECDSGNFGRDSSSPNS*WRRCSKGQTWYSTWSEGSSERKRDQGKKLTIL 183
Query: 305 RRSICPFQCQHPRKFDV*T 249
RSICPFQC P +FD T
Sbjct: 184 WRSICPFQCHRPYEFDTKT 240
Score = 150 bits (322), Expect(2) = 3e-036
Identities = 65/76 (85%), Positives = 68/76 (89%)
Frame = +2 / -3
Query: 260 QIFGDVDIEMDILISVVR*VSCLDPFSSQNYPLTRLSTGLDL*NNVSTESWDLNCPSQNC 439
QI D DIEMDILIS+V *VSCLDPFSSQNY LTRLST DL*NNVST+SWDLNCPSQNC
Sbjct: 229 QIHRDGDIEMDILISIVW*VSCLDPFSSQNYLLTRLSTRFDL*NNVSTKSWDLNCPSQNC 50
Query: 440 LSHIQDSINMNITFLS 487
LSHIQ+SINMNITF S
Sbjct: 49 LSHIQNSINMNITFFS 2
Score = 133 bits (284), Expect = 1e-029
Identities = 63/72 (87%), Positives = 65/72 (90%)
Frame = -3 / +3
Query: 486 ERKVIFMLMLS*M*LRQFWEGQFKSQLSVETLF*RSSPVLNLVRG*F*EEKGSRQETHRT 307
E+KVIFMLML *M*LRQFWEGQFKSQL VETLF*RS+ VLNLVR *F*EEKGSRQETH T
Sbjct: 3 EKKVIFMLMLF*M*LRQFWEGQFKSQLLVETLF*RSNLVLNLVRR*F*EEKGSRQETHHT 182
Query: 306 TEINMSISMSTS 271
EINMSISMS S
Sbjct: 183 MEINMSISMSPS 218
Score = 113 bits (242), Expect(2) = 5e-025
Identities = 53/76 (69%), Positives = 54/76 (71%)
Frame = +1 / -2
Query: 259 SNFRGC*H*NGHIDLRGTVXXXXXXXXXXXXPSDQVEYRA*SLEQRLH*ELGLELSLPKL 438
SN G *H*NGHIDL V PSDQVEY+ * LEQRLH*ELGLELSLPKL
Sbjct: 230 SNS*GR*H*NGHIDLHSMVSFLP*SLFLSELPSDQVEYQV*PLEQRLH*ELGLELSLPKL 51
Query: 439 PESHSGQHQHEYHLSL 486
PESHS QHQHEYHL L
Sbjct: 50 PESHSEQHQHEYHLFL 3
Score = 29.5 bits (58), Expect(2) = 1e-048
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = -2 / +1
Query: 70 IGLCFGFCSWN 38
I LC GFCSWN
Sbjct: 460 IDLCLGFCSWN 492
Score = 28.6 bits (56), Expect(2) = 8e-041
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = +3 / -2
Query: 12 ISSRRIIHLFQLQNPKQRPI 71
IS + LFQLQNPKQR I
Sbjct: 518 ISGTGDLVLFQLQNPKQRSI 459
Score = 27.2 bits (53), Expect(2) = 3e-036
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +2 / -1
Query: 32 SFIPATKSETKTYL 73
S IPATKS+TK Y+
Sbjct: 498 SIIPATKSQTKIYM 457
Score = 26.3 bits (51), Expect(2) = 5e-025
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +1 / -3
Query: 37 YSSYKIRNKDLSI 75
YSSYKI NKDL +
Sbjct: 493 YSSYKIPNKDLYV 455
>gb|CD212886.1|CD212886 HS1_17_D02.b1_A012 Heat-shocked seedlings Sorghum bicolor cDNA
clone HS1_17_D02_A012 3', mRNA sequence
Length = 646
Score = 149 bits (320), Expect(3) = 1e-043
Identities = 61/69 (88%), Positives = 64/69 (92%)
Frame = -2 / +3
Query: 433 LGGTVQVPTLSGDVVLKIKPGTQPGQRVVLRGKGIKTRNSPYHGDQYVHFNVNIPENLTS 254
LGGTVQVPTLSGDVVLK+KPGTQPGQ+VVLRGKGIK RNSPY+GDQYVHFNV IP NLT
Sbjct: 54 LGGTVQVPTLSGDVVLKVKPGTQPGQKVVLRGKGIKARNSPYYGDQYVHFNVTIPVNLTP 233
Query: 253 RQRELIEEF 227
RQR LIEEF
Sbjct: 234 RQRVLIEEF 260
Score = 134 bits (287), Expect(3) = 2e-039
Identities = 56/64 (87%), Positives = 59/64 (92%)
Frame = +3 / -1
Query: 246 RCLDVKFSGMLTLKWTY*SPWYGEFLVLIPFPLRTTL*PG*VPGLIFRTTSPLRVGT*TV 425
RCL VKF+GM+TLKWTY*SP YGEFL LIPFPLRTT *PG*VPGL FRTTSPLRVGT*TV
Sbjct: 241 RCLGVKFTGMVTLKWTY*SP*YGEFLALIPFPLRTTF*PG*VPGLTFRTTSPLRVGT*TV 62
Query: 426 PPKI 437
PPK+
Sbjct: 61 PPKL 50
Score = 116 bits (247), Expect(2) = 4e-032
Identities = 48/62 (77%), Positives = 50/62 (80%)
Frame = -1 / +2
Query: 434 FGRDSSSPNSQWRRCSKDQARYSTWSEGSSERKRDQDKKLTVPRRSICPFQCQHPRKFDV 255
FGRDSSSPNS WRRCSK Q YSTWSEGSSERKRDQ KKLT+ RSICPFQ HP +FD
Sbjct: 53 FGRDSSSPNS*WRRCSKGQTWYSTWSEGSSERKRDQGKKLTILWRSICPFQRHHPCEFDT 232
Query: 254 *T 249
T
Sbjct: 233 KT 238
Score = 111 bits (236), Expect(3) = 5e-031
Identities = 48/58 (82%), Positives = 51/58 (87%)
Frame = +2 / -3
Query: 260 QIFGDVDIEMDILISVVR*VSCLDPFSSQNYPLTRLSTGLDL*NNVSTESWDLNCPSQ 433
QI D D+EMDILIS+V *VSCLDPFSSQNY LTRLST DL*NNVST+SWDLNCPSQ
Sbjct: 227 QIHRDGDVEMDILISIVW*VSCLDPFSSQNYLLTRLSTRFDL*NNVSTKSWDLNCPSQ 54
Score = 98.7 bits (209), Expect(2) = 2e-024
Identities = 46/54 (85%), Positives = 47/54 (87%)
Frame = -3 / +1
Query: 432 WEGQFKSQLSVETLF*RSSPVLNLVRG*F*EEKGSRQETHRTTEINMSISMSTS 271
WEGQFKSQL VETLF*RS+ VLNLVR *F*EEKGSRQETH T EINMSIS S S
Sbjct: 55 WEGQFKSQLLVETLF*RSNLVLNLVRR*F*EEKGSRQETHHTMEINMSISTSPS 216
Score = 48.7 bits (100), Expect(3) = 2e-039
Identities = 18/19 (94%), Positives = 18/19 (94%)
Frame = +1 / -1
Query: 430 PKLPESHSGQHQHEYHLSL 486
PKLPESHS QHQHEYHLSL
Sbjct: 58 PKLPESHSEQHQHEYHLSL 2
Score = 46.9 bits (96), Expect(3) = 1e-043
Identities = 17/19 (89%), Positives = 17/19 (89%)
Frame = -1 / +3
Query: 485 RER*YSC*CCPECDSGNFG 429
RER*YSC*CC ECDSGN G
Sbjct: 3 RER*YSC*CCSECDSGNLG 59
Score = 44.6 bits (91), Expect(2) = 4e-032
Identities = 19/21 (90%), Positives = 19/21 (90%)
Frame = -2 / +1
Query: 487 REKGDIHVDAVLNVTQAILGG 425
REKGDIHVDAVLNVTQAI G
Sbjct: 1 REKGDIHVDAVLNVTQAIWEG 63
Score = 43.7 bits (89), Expect(3) = 5e-031
Identities = 17/18 (94%), Positives = 18/18 (100%)
Frame = +2 / -2
Query: 434 NCLSHIQDSINMNITFLS 487
NCLSHIQ+SINMNITFLS
Sbjct: 54 NCLSHIQNSINMNITFLS 1
Score = 35.9 bits (72), Expect(2) = 2e-024
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = -3 / +2
Query: 486 ERKVIFMLMLS*M*LRQF 433
ERKVIFMLML *M*LRQF
Sbjct: 2 ERKVIFMLMLF*M*LRQF 55
Score = 26.3 bits (51), Expect(3) = 1e-043
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = -2 / +3
Query: 70 IGLCFGFCSWN 38
I LC G CSWN
Sbjct: 459 IDLCLGVCSWN 491
Score = 25.8 bits (50), Expect(3) = 2e-039
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / -1
Query: 12 ISSRRIIHLFQLQNPKQRPI 71
IS + LFQLQ PKQR I
Sbjct: 517 ISGTGDLVLFQLQTPKQRSI 458
Score = 25.4 bits (49), Expect(3) = 5e-031
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +1 / -2
Query: 37 YSSYKIRNKDLSI 75
YSSYK+ NKDL +
Sbjct: 492 YSSYKLPNKDLYV 454
>gb|BG557893.1|BG557893 EM1_55_H09.b1_A002 Embryo 1 (EM1) Sorghum bicolor cDNA, mRNA sequence
Length = 453
Score = 167 bits (359), Expect(2) = 6e-042
Identities = 72/90 (80%), Positives = 74/90 (82%)
Frame = +1 / -1
Query: 1084 IMIFFVHPLESHKLVEPLETCVLHPHHLYLCLDGASLQAHNRPF*SPPGWRSC*RRAHRS 1263
IMIFFVHP +SHKLVEPLET VLH HHLY CLD ASLQAHNRPF* G RS *RRAHRS
Sbjct: 453 IMIFFVHPSKSHKLVEPLETSVLHLHHLYSCLDEASLQAHNRPF*CLIG*RSY*RRAHRS 274
Query: 1264 SPLPPPAWSRGRTCGRCSADRRESSGRAHR 1353
SPLPPPAW RGRTCGR S RR +SGRA R
Sbjct: 273 SPLPPPAWFRGRTCGRYSVTRRANSGRAPR 184
Score = 163 bits (350), Expect(2) = 6e-039
Identities = 67/70 (95%), Positives = 70/70 (100%)
Frame = -2 / +3
Query: 1294 HGTRPVAARDYYDVLGVSKSASQAEIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 1115
HGTRPVAARDYYDVLGVSK+ASQ++IKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE
Sbjct: 243 HGTRPVAARDYYDVLGVSKNASQSDIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 422
Query: 1114 TLKDEQKRSL 1085
TLKDEQKRSL
Sbjct: 423 TLKDEQKRSL 452
Score = 151 bits (324), Expect = 3e-035
Identities = 64/87 (73%), Positives = 66/87 (75%)
Frame = -3 / +1
Query: 1344 PPGALPSIRRAAPAGPSTGPGRWRQGTTTMCXXXXXXXXXXXSKRPIMGLQRSSIQTQIK 1165
PPG PS R AP G ST PGRWRQGTTTMC SKRPIMGLQRS IQT+IK
Sbjct: 193 PPGVRPSRHRVAPTGSSTEPGRWRQGTTTMCSASVRTLANQTSKRPIMGLQRSFIQTRIK 372
Query: 1164 VMRMQNASFKRFNELMRL*RMNKKDHY 1084
VM+MQN SFKRFNELMRL*RMNKKDHY
Sbjct: 373 VMQMQNGSFKRFNELMRL*RMNKKDHY 453
Score = 142 bits (304), Expect = 2e-032
Identities = 63/78 (80%), Positives = 67/78 (85%)
Frame = -1 / +2
Query: 1346 ALPELSLLSAEQRPQVLPRDQAGGGKGLLRCARRQQERQPGGDQKGLLWACKEAPSRHK* 1167
ALPE +LL E RPQVLPR+QAGGGKGLLRCARRQ ER P QKGLLWACKEA SRH+*
Sbjct: 191 ALPEFALLVTE*RPQVLPRNQAGGGKGLLRCARRQ*ER*PIRHQKGLLWACKEASSRHE* 370
Query: 1166 R*CGCRTQVSRGSTSL*D 1113
R*C CRT+VSRGSTSL*D
Sbjct: 371 R*CRCRTEVSRGSTSL*D 424
Score = 123 bits (263), Expect = 8e-027
Identities = 51/66 (77%), Positives = 53/66 (80%)
Frame = +2 / -2
Query: 1148 FCIRITFICVWMELLCKPIIGLFDLRLAGALADAEHIVVVPCRHRPGPVEGPAGAARRIE 1327
FCI ITFI VWM+LLCKPIIGLFD+ LA L DAEHIVVVPCRHRPG VE P GA R E
Sbjct: 389 FCICITFIRVWMKLLCKPIIGLFDV*LASVLTDAEHIVVVPCRHRPGSVEEPVGATR*RE 210
Query: 1328 GRAPGG 1345
GR PGG
Sbjct: 209 GRTPGG 192
Score = 26.3 bits (51), Expect(2) = 6e-042
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = +3 / -3
Query: 1410 ASRSKNRTPAWRRMAVA 1460
ASRS R PA RMAVA
Sbjct: 103 ASRSARRAPAGSRMAVA 53
Score = 23.1 bits (44), Expect(2) = 6e-039
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = -1 / +2
Query: 1478 TTSQPPRHSHAPPRRGT 1428
T + RH HA PRR T
Sbjct: 35 TRAPVSRHRHAAPRRRT 85
>gb|CD203946.1|CD203946 HS1_2_D07.g1_A012 Heat-shocked seedlings Sorghum bicolor cDNA clone
HS1_2_D07_A012 5', mRNA sequence
Length = 572
Score = 150 bits (322), Expect = 6e-035
Identities = 60/83 (72%), Positives = 68/83 (81%)
Frame = -2 / +1
Query: 1294 HGTRPVAARDYYDVLGVSKSASQAEIKKAYYGLAKKLHPDTNKGDADAERKFQEVQRAYE 1115
H TR V ARD+YDVLGVSK AS +IKKAYY LAKK HPDTNK DADAE+KFQEV RAYE
Sbjct: 322 HATRRVYARDFYDVLGVSKDASAPDIKKAYYALAKKFHPDTNKDDADAEKKFQEVNRAYE 501
Query: 1114 TLKDEQKRSLYDQVGPDQYEKTA 1046
LKD+ KR +YDQ+GP+ YE+ A
Sbjct: 502 VLKDDDKREIYDQLGPEAYERQA 570
>gb|CD203846.1|CD203846 HS1_2_D07.b1_A012 Heat-shocked seedlings Sorghum bicolor cDNA clone
HS1_2_D07_A012 3', mRNA sequence
Length = 646
Score = 141 bits (302), Expect = 3e-032
Identities = 54/80 (67%), Positives = 69/80 (86%)
Frame = -2 / +1
Query: 466 VDAVLNVTQAILGGTVQVPTLSGDVVLKIKPGTQPGQRVVLRGKGIKTRNSPYHGDQYVH 287
VD+VL++ QA+LGGTV VPTL+G+V +K++ GTQPG++VVLRGKGIK RNSP G+QYVH
Sbjct: 1 VDSVLSIAQAVLGGTVTVPTLTGNVTVKVRQGTQPGEKVVLRGKGIKARNSPVFGNQYVH 180
Query: 286 FNVNIPENLTSRQRELIEEF 227
FN+ +P +T RQRELIEEF
Sbjct: 181 FNIRVPTEVTQRQRELIEEF 240
>gb|CW141260.1|CW141260 104_532_11136222_116_34935_029 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11136222, DNA
sequence
Length = 739
Score = 71.6 bits (150), Expect(3) = 5e-021
Identities = 29/30 (96%), Positives = 29/30 (96%)
Frame = -2 / -1
Query: 1198 LAKKLHPDTNKGDADAERKFQEVQRAYETL 1109
LAKKLHPDTNKGDADAERKFQEVQRAYE L
Sbjct: 496 LAKKLHPDTNKGDADAERKFQEVQRAYEVL 407
Score = 39.6 bits (80), Expect(3) = 5e-021
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -1 / -1
Query: 1073 WP*SV*ENCFWRRRNWL 1023
W *SV*E+CFW RRNWL
Sbjct: 55 WS*SV*ESCFWWRRNWL 5
Score = 35.4 bits (71), Expect(3) = 5e-021
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = -3 / -1
Query: 1116 RL*RMNKKDHYMIR 1075
RL*RMNKKDHYMIR
Sbjct: 319 RL*RMNKKDHYMIR 278
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,873,234,442
Number of Sequences: 832831
Number of extensions: 30598960
Number of successful extensions: 260000
Number of sequences better than 1.0e-020: 12
length of database: 163,786,556
effective HSP length: 56
effective length of database: 117,148,020
effective search space used: 54473829300
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)