TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448720.2.1
(510 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BZ422000.1|BZ422000 id48d07.b1 WGS-SbicolorF (DH5a methy... 127 7e-072
>gb|BZ422000.1|BZ422000 id48d07.b1 WGS-SbicolorF (DH5a methyl filtered) Sorghum bicolor
genomic clone id48d07 5', DNA sequence
Length = 706
Score = 127 bits (272), Expect(4) = 7e-072
Identities = 48/52 (92%), Positives = 52/52 (100%)
Frame = +3 / +3
Query: 24 VGVWNLRAERLDDWYRGQEVYVKVADPLGYNITEMVVPDNALYCGLLKERQK 179
VG+WNLRAE+LD+WYRGQEVYVKVADPLGYNITEMV+PDNALYCGLLKERQK
Sbjct: 3 VGIWNLRAEKLDNWYRGQEVYVKVADPLGYNITEMVIPDNALYCGLLKERQK 158
Score = 107 bits (229), Expect(4) = 1e-064
Identities = 41/51 (80%), Positives = 46/51 (90%)
Frame = -1 / -2
Query: 177 FASPSVDHSRGHCPGRPSR*CCSPVDRQPSHRPPALCTSHPAFRRADSRHQ 25
FASPSVDHS GH PG P +*CCSPVD+QPSHRPPALCTS+PAF+ ADSR+Q
Sbjct: 156 FASPSVDHSTGHYPG*PFQ*CCSPVDQQPSHRPPALCTSYPAFQHADSRYQ 4
Score = 105 bits (224), Expect(3) = 4e-049
Identities = 47/52 (90%), Positives = 47/52 (90%)
Frame = -3 / -1
Query: 178 FCLSFSRPQ*RALSGTTISVML*PSGSATFT*TSCPLYQSSSLSARRFQTPT 23
FCLSFSRPQ RALSG TISVML*PSGSATFT*TSCPLYQ SS SARRFQ PT
Sbjct: 157 FCLSFSRPQYRALSGMTISVML*PSGSATFT*TSCPLYQLSSFSARRFQIPT 2
Score = 102 bits (218), Expect(4) = 8e-055
Identities = 43/52 (82%), Positives = 45/52 (86%)
Frame = +1 / +1
Query: 25 LVSGICAPKGWMTGTEGRRSM*RLPIHWATTSPRWSSRTMPSTVVY*RRGKS 180
LVSGIC K W+TGTEGRRSM*RL IHWATTS +WSSR MP TVVY*RRGKS
Sbjct: 4 LVSGICVLKSWITGTEGRRSM*RLLIHWATTSLKWSSRIMPCTVVY*RRGKS 159
Score = 101 bits (216), Expect(3) = 6e-039
Identities = 42/51 (82%), Positives = 44/51 (86%)
Frame = +2 / +2
Query: 26 WCLESARRKAG*LVQRAGGLCEGCRSTGLQHHRDGRPGQCPLLWSTEGEAK 178
W LESA KAG*LVQRAGGLCEGC STGLQHH +G PG CP+LWSTEGEAK
Sbjct: 5 WYLESAC*KAG*LVQRAGGLCEGC*STGLQHH*NGHPG*CPVLWSTEGEAK 157
Score = 94.1 bits (199), Expect(3) = 7e-042
Identities = 37/45 (82%), Positives = 39/45 (86%)
Frame = -2 / -3
Query: 158 TTVEGIVRDDHLGDVVAQWIGNLHIDLLPSVPVIQPFGAQIPDTN 24
TTV+GI+RDDH DVVAQWI NLHIDLLPSVPVIQ F QIPDTN
Sbjct: 137 TTVQGIIRDDHFSDVVAQWISNLHIDLLPSVPVIQLFSTQIPDTN 3
Score = 93.6 bits (198), Expect(4) = 7e-072
Identities = 38/45 (84%), Positives = 41/45 (91%)
Frame = +2 / +1
Query: 176 KATGS*VEQQVVGARRCWMEQPTPRNHDPGRRSCDLQLILSLFFS 310
+ATGS*V+QQVVGARRCWME TPRNHD GRRSCDLQLILS FF+
Sbjct: 247 QATGS*VKQQVVGARRCWMELQTPRNHDAGRRSCDLQLILSKFFA 381
Score = 85.8 bits (181), Expect(4) = 1e-064
Identities = 36/43 (83%), Positives = 36/43 (83%)
Frame = -1 / -3
Query: 306 KKRERIN*RSQLRRPGSWLRGVGCSIQHRLAPTTCCSTHEPVA 178
K ERIN*RSQLRRP SWLRGV SIQH LAPTTCC THEPVA
Sbjct: 377 KNFERIN*RSQLRRPASWLRGVCSSIQHLLAPTTCCLTHEPVA 249
Score = 79.4 bits (167), Expect(3) = 4e-049
Identities = 36/46 (78%), Positives = 39/46 (84%)
Frame = -3 / -2
Query: 295 EDQLKITAATTRIMVARSRLLHPASPCADDLLFDS*TCGFCLSFSR 158
++QLKITAATT IMVARS LHPASPCADDLLFDS*TCG +SR
Sbjct: 366 KNQLKITAATTSIMVARSL*LHPASPCADDLLFDS*TCGLQGFWSR 229
Score = 71.2 bits (149), Expect(3) = 7e-042
Identities = 29/37 (78%), Positives = 32/37 (86%)
Frame = -2 / -1
Query: 308 KKKERGSTEDHSCDDQDHGCEESVAPSSIALRRRLAV 198
+K + STEDHSCDDQ HGCEE VAPSSI+LRRRLAV
Sbjct: 379 QKTLKESTEDHSCDDQHHGCEEFVAPSSISLRRRLAV 269
Score = 62.9 bits (131), Expect(4) = 1e-064
Identities = 27/32 (84%), Positives = 27/32 (84%)
Frame = -1 / -3
Query: 408 PDPISTNYPMLNL*ARRFNVLFPLGKNLPKNF 313
PDPISTNYPMLNL* R FNVLF L KNL KNF
Sbjct: 464 PDPISTNYPMLNL*GRCFNVLFLLRKNLAKNF 369
Score = 62.9 bits (131), Expect(4) = 8e-055
Identities = 26/33 (78%), Positives = 30/33 (90%)
Frame = +3 / +2
Query: 159 LLKERQKPQVHESNSKSSAQGDAGWSNRLLATM 257
LL++ +PQVHESNSKSSAQGDAGWS +LLATM
Sbjct: 230 LLQKPCRPQVHESNSKSSAQGDAGWSYKLLATM 328
Score = 62.0 bits (129), Expect(4) = 8e-055
Identities = 27/32 (84%), Positives = 27/32 (84%)
Frame = +2 / +1
Query: 314 KFFGRFLPRGNRTLKRLAYKFSIG*FVEIGSG 409
KFF RFL R NRTLK L YKFSIG*FVEIGSG
Sbjct: 370 KFFARFLRRRNRTLKHLPYKFSIG*FVEIGSG 465
Score = 62.0 bits (129), Expect(4) = 7e-072
Identities = 27/32 (84%), Positives = 30/32 (93%)
Frame = +1 / +3
Query: 313 KVFW*IFA*RK*NIETSSLQVQHRVVRGDRIW 408
KVF *IFA*+K*NIETS+LQVQHRV+ GDRIW
Sbjct: 369 KVFC*IFA*KK*NIETSTLQVQHRVICGDRIW 464
Score = 52.8 bits (109), Expect(3) = 6e-039
Identities = 23/42 (54%), Positives = 29/42 (69%)
Frame = +3 / +2
Query: 327 DFCLEEIEH*NV*PTSSA*GSSWR*DLAELFDSVGQLGMMSV 452
DFC+EEIEH*N+ TSSA*G+ WR*DL V +G + +
Sbjct: 383 DFCVEEIEH*NIYLTSSA*GNLWR*DLVMTLKLVDSVGQLEM 508
Score = 49.2 bits (101), Expect(3) = 4e-049
Identities = 22/34 (64%), Positives = 24/34 (70%)
Frame = -3 / -2
Query: 439 PSCPTLSNSSARSYLHELPYAELVG*TFQCSISS 338
P+ T RSYLH+LPYAELV * FQCSISS
Sbjct: 495 PTLSTSLRVITRSYLHKLPYAELVR*MFQCSISS 394
Score = 48.3 bits (99), Expect(4) = 1e-064
Identities = 21/32 (65%), Positives = 23/32 (71%)
Frame = -3 / -2
Query: 508 NTHRITDKSHSSITFLAFSTDIIPSCPTLSNS 413
NTH + SH SI LAFST+II SCPTLS S
Sbjct: 573 NTHWVLLNSHLSIMLLAFSTNIISSCPTLSTS 478
Score = 46.0 bits (94), Expect(4) = 7e-072
Identities = 20/33 (60%), Positives = 27/33 (81%)
Frame = +3 / +2
Query: 411 ELFDSVGQLGMMSVEKARNVILE*DLSVIRCVF 509
+L DSVGQL MM VEKAR++IL+*+ + +CVF
Sbjct: 476 KLVDSVGQLEMMFVEKARSMILK*EFNKTQCVF 574
Score = 46.0 bits (94), Expect(3) = 7e-042
Identities = 16/24 (66%), Positives = 18/24 (75%)
Frame = -2 / -1
Query: 482 SFQYHVSCLLHRHHSKLPYTIKQL 411
S +YH SCL H+HH KL YTI QL
Sbjct: 547 SLKYHASCLFHKHHFKLSYTINQL 476
Score = 45.1 bits (92), Expect(3) = 6e-039
Identities = 18/21 (85%), Positives = 20/21 (95%)
Frame = +1 / +3
Query: 181 HRFMSRTASRRRKAMLDGATD 243
HRFMS+TASRRRK MLDGAT+
Sbjct: 252 HRFMSQTASRRRKEMLDGATN 314
Score = 43.7 bits (89), Expect(4) = 8e-055
Identities = 20/33 (60%), Positives = 23/33 (69%)
Frame = +2 / +1
Query: 410 RAV**CRAAWNDVCGEGKKRDTGMRFISYPVRV 508
+A **CR NDVCG+GKK DT +R PVRV
Sbjct: 475 QAG**CRTT*NDVCGKGKKHDT*VRI*QNPVRV 573
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 655,097,960
Number of Sequences: 832831
Number of extensions: 10119144
Number of successful extensions: 167828
Number of sequences better than 1.0e-020: 1
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 13878995508
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)