TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2276425.2.1
(753 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN127328.1|CN127328 RHOH1_22_H02.b1_A002 Acid- and alkal... 303 e-111
gb|BG103631.1|BG103631 RHIZ2_38_H11.g1_A003 Rhizome2 (RHIZ2... 222 5e-057
gb|CD227098.1|CD227098 CCC1_4_E12.b1_A007 Callus culture/ce... 196 4e-049
gb|CW060796.1|CW060796 104_304_10520052_115_30144 Sorghum m... 194 1e-048
gb|CW261852.1|CW261852 104_729_11229737_116_35209_077 Sorgh... 194 1e-048
gb|CW295416.1|CW295416 104_776_11460918_116_35617_019 Sorgh... 194 1e-048
gb|CN130358.1|CN130358 RHOH1_41_A07.b1_A002 Acid- and alkal... 194 1e-048
gb|CW444261.1|CW444261 fsbb001f164m08k0 Sorghum methylation... 172 6e-042
gb|CW216402.1|CW216402 104_649_11195097_148_37462_043 Sorgh... 103 1e-041
gb|CW069905.1|CW069905 104_320_10526223_1_30089 Sorghum met... 103 1e-041
gb|CW217099.1|CW217099 104_650_11195476_148_37131_012 Sorgh... 100 6e-040
gb|CW485924.1|CW485924 fsbb001f248o11k0 Sorghum methylation... 159 4e-038
gb|CW295417.1|CW295417 104_776_11460918_148_35613_019 Sorgh... 157 1e-037
gb|CW227932.1|CW227932 104_667_11206082_116_37242_005 Sorgh... 75 3e-033
gb|CW217098.1|CW217098 104_650_11195476_116_37132_012 Sorgh... 103 1e-031
gb|CW038350.1|CW038350 104_270_10504872_115_30386 Sorghum m... 131 1e-029
gb|CW058700.1|CW058700 104_301_10518886_114_30160 Sorghum m... 103 4e-021
gb|CW059684.1|CW059684 104_302_10519462_114_30165 Sorghum m... 103 4e-021
>gb|CN127328.1|CN127328 RHOH1_22_H02.b1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_22_H02_A002 3', mRNA sequence
Length = 775
Score = 303 bits (655), Expect(3) = e-111
Identities = 124/129 (96%), Positives = 125/129 (96%)
Frame = +2 / +2
Query: 179 KPCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKD 358
KPCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKD
Sbjct: 302 KPCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKD 481
Query: 359 ELGLLHPAFKAVKTST**LEYNSTCYFLGSF*ILLGLNPKCSFCRIG*QQKFGLGLWDGY 538
ELGLLHPAFKAVKTST**LEYNSTCYF+GSF*ILLGLNPKCSFCRI QQKFGLG WDGY
Sbjct: 482 ELGLLHPAFKAVKTST**LEYNSTCYFVGSF*ILLGLNPKCSFCRIWWQQKFGLGSWDGY 661
Query: 539 LCCNVRC*E 565
LCCNVR *E
Sbjct: 662 LCCNVRY*E 688
Score = 259 bits (560), Expect(3) = 1e-091
Identities = 105/133 (78%), Positives = 112/133 (84%)
Frame = +1 / +1
Query: 172 RVQAVWQIWRLPCRAMPQEYGPCIQFWG*PDLADVWVHTQVTSKXXXXXXXXXXXXPT*D 351
R QA+WQIWRLPCRAMPQEYG C+QFW *PDLADVW+H+QVT K P *D
Sbjct: 295 RHQAMWQIWRLPCRAMPQEYGSCVQFW**PDLADVWIHSQVTCKQESEEDQE*DQQPA*D 474
Query: 352 KG*AWVASSSIQGCEDFHMIIRVQFNLLFSWFVLDPVGSKSKVFFL*NRVTTEVWVGVMG 531
KG*AWVASS IQGCEDFHMIIRVQF+LLF WFVLDPVGSKSKVFFL*N V TEVWVG+MG
Sbjct: 475 KG*AWVASSGIQGCEDFHMIIRVQFHLLFCWFVLDPVGSKSKVFFL*NLVATEVWVGIMG 654
Query: 532 WISLL*CEVLGEL 570
WISLL*CEVLGE+
Sbjct: 655 WISLL*CEVLGEM 693
Score = 255 bits (551), Expect(3) = 3e-079
Identities = 113/130 (86%), Positives = 117/130 (90%)
Frame = +3 / +3
Query: 177 ASRVANLETTLSSDASRIWTVHSILGITRSCRCMGSHTSH*QAGE*RGSGTRPATHLRQR 356
+S VANLETTLSSDASRIW V SIL ITRSCRCM S TSH QAGE*RGSG RPAT LRQR
Sbjct: 300 SSHVANLETTLSSDASRIWIVRSILVITRSCRCMDSLTSHLQAGE*RGSGMRPATRLRQR 479
Query: 357 MSLGCFIQHSRL*RLPHDN*STIQLVIFLVRSRSCWV*IQSVLFVESGDNRSLGWGYGMD 536
MSLGCFI+HSRL*RLPHDN*STI LVI LVRSRSCWV*IQSVLFVESG NRSLGW +GMD
Sbjct: 480 MSLGCFIRHSRL*RLPHDN*STIPLVILLVRSRSCWV*IQSVLFVESGGNRSLGWDHGMD 659
Query: 537 IFVVM*GVRR 566
IFVVM*G+RR
Sbjct: 660 IFVVM*GIRR 689
Score = 251 bits (543), Expect(3) = 5e-091
Identities = 114/129 (88%), Positives = 116/129 (89%)
Frame = -3 / -1
Query: 565 LLTPHITTKISIP*PQPKLLLSPDSTKRTLWI*TQQDLERTKKITS*IVL*LSCGSLHSL 386
LL PHITTKISIP* QPKLLL PDSTKRTLWI*TQQDLERT KITS IVL*LSCGSLHSL
Sbjct: 688 LLIPHITTKISIP*SQPKLLLPPDSTKRTLWI*TQQDLERTNKITSGIVL*LSCGSLHSL 509
Query: 385 ECWMKQPKLILCLKWVAGLVPDPLHSPAC**LVCEPIHLQDLVIPKIECTVHILEASLDR 206
EC MKQPKLILCLK VAGL+PDPLHSPAC *LV E IHLQDLVI KIE T+HILEASLDR
Sbjct: 508 ECRMKQPKLILCLKRVAGLIPDPLHSPACK*LVSESIHLQDLVITKIERTIHILEASLDR 329
Query: 205 VVSKFATRL 179
VVSKFAT L
Sbjct: 328 VVSKFATWL 302
Score = 165 bits (355), Expect(4) = 1e-084
Identities = 66/72 (91%), Positives = 68/72 (94%)
Frame = -2 / -3
Query: 566 SPNTSHYNKDIHPITPTQTSVVTRFYKKNTLDLDPTGSRTNQENNKLNCTLIIMWKSSQP 387
SPNTSHYNKDIHP+ PTQTSV TRFYKKNTLDLDPTGSRTNQ+NNK NCTLIIMWKSSQP
Sbjct: 689 SPNTSHYNKDIHPMIPTQTSVATRFYKKNTLDLDPTGSRTNQQNNKWNCTLIIMWKSSQP 510
Query: 386 *MLDEATQAHPL 351
*M DEATQAHPL
Sbjct: 509 *MPDEATQAHPL 474
Score = 159 bits (341), Expect(4) = 8e-062
Identities = 64/68 (94%), Positives = 66/68 (97%)
Frame = -1 / -2
Query: 567 FS*HLTLQQRYPSHNPNPNFCCHPILQKEHFGFRPNRI*NEPRK*QVELYSNYHVEVFTA 388
FS*+LTLQQRYPSH+PNPNFCCH ILQKEHFGFRPNRI*NEP K*QVELYSNYHVEVFTA
Sbjct: 690 FS*YLTLQQRYPSHDPNPNFCCHQILQKEHFGFRPNRI*NEPTK*QVELYSNYHVEVFTA 511
Query: 387 LNAG*SNP 364
LNAG*SNP
Sbjct: 510 LNAG*SNP 487
Score = 96.8 bits (205), Expect(3) = e-111
Identities = 36/37 (97%), Positives = 36/37 (97%)
Frame = +2 / +3
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEM 112
VYILATQREKWGDK YLENGYYLHGYWGILVDRYEEM
Sbjct: 144 VYILATQREKWGDKVYLENGYYLHGYWGILVDRYEEM 254
Score = 91.8 bits (194), Expect(4) = 1e-084
Identities = 36/36 (100%), Positives = 36/36 (100%)
Frame = -1 / -2
Query: 285 VNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGL 178
VNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGL
Sbjct: 408 VNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGL 301
Score = 80.8 bits (170), Expect(3) = 5e-091
Identities = 35/37 (94%), Positives = 35/37 (94%)
Frame = -1 / -1
Query: 111 ISSYLSTKIPQ*PWR*YPFSR*ALSPHFSRCVARMYT 1
ISSYLSTKIPQ*P R*YPFSR* LSPHFSRCVARMYT
Sbjct: 253 ISSYLSTKIPQ*P*R*YPFSR*TLSPHFSRCVARMYT 143
Score = 73.9 bits (155), Expect(4) = 1e-084
Identities = 30/38 (78%), Positives = 35/38 (92%)
Frame = -3 / -3
Query: 115 KHLLVPVDQDPPVAVEVVPILKVSLITPFLALCREDVH 2
+HLL+PVDQDPPVA++VVPILKV+LITP LAL EDVH
Sbjct: 257 RHLLIPVDQDPPVAMKVVPILKVNLITPLLALRCEDVH 144
Score = 73.9 bits (155), Expect(3) = 1e-091
Identities = 32/38 (84%), Positives = 34/38 (89%)
Frame = +1 / +2
Query: 1 GVHPRDTAREMG**GLP*EWVLPPRLLGDLGRQVRGDA 114
GVHPR+ ARE+G**GLP*EWVLP LLGDLGRQV GDA
Sbjct: 143 GVHPRNAAREVG**GLP*EWVLPSWLLGDLGRQV*GDA 256
Score = 61.6 bits (128), Expect(4) = 8e-062
Identities = 26/30 (86%), Positives = 26/30 (86%)
Frame = -2 / -3
Query: 269 ARSGYPQN*MHGPYS*GIARQGSLQICHTA 180
ARSGY QN* H PYS*GIARQGSLQICH A
Sbjct: 392 ARSGYHQN*THDPYS*GIARQGSLQICHMA 303
Score = 45.1 bits (92), Expect(3) = e-111
Identities = 18/20 (90%), Positives = 18/20 (90%)
Frame = +2 / +3
Query: 566 NYVMFIYWHKLCLTLVETS* 625
N VMFIYWHKLCLTLVE S*
Sbjct: 690 NDVMFIYWHKLCLTLVEMS* 749
Score = 45.1 bits (92), Expect(3) = 3e-079
Identities = 21/46 (45%), Positives = 23/46 (50%)
Frame = +3 / +1
Query: 3 CTSSRHSARNGVIRLTLRMXXXXXXXXXXXXXXXRRCLRITSQGSA 140
CTSS+ SAR+GVIR TLRM RRCL G A
Sbjct: 145 CTSSQRSARSGVIRFTLRMGTTFMATGGSWSTGMRRCLAPPPPGDA 282
Score = 44.1 bits (90), Expect(3) = 1e-091
Identities = 19/27 (70%), Positives = 22/27 (81%)
Frame = +1 / +2
Query: 571 CYVYLLA*VMSYARRNELMDELKLLVA 651
CYVYLLA*VMSY RRNEL +++VA
Sbjct: 695 CYVYLLA*VMSYTRRNELNGWTQVIVA 775
Score = 43.2 bits (88), Expect(3) = 5e-091
Identities = 18/20 (90%), Positives = 18/20 (90%)
Frame = -3 / -3
Query: 625 LTRFYERKT*LMPINKHNII 566
LT FYE KT*LMPINKHNII
Sbjct: 749 LTHFYECKT*LMPINKHNII 690
Score = 41.4 bits (84), Expect(4) = 1e-084
Identities = 18/20 (90%), Positives = 19/20 (95%)
Frame = -1 / -1
Query: 624 *LVSTSVRHNLCQ*INIT*F 565
*L+STSVRHNLCQ*INIT F
Sbjct: 748 *LISTSVRHNLCQ*INITSF 689
Score = 39.6 bits (80), Expect(4) = 8e-062
Identities = 14/17 (82%), Positives = 14/17 (82%)
Frame = -2 / -2
Query: 53 QGKPYHPISRAVSRGCT 3
QGKPYHP SRA RGCT
Sbjct: 195 QGKPYHPTSRAALRGCT 145
Score = 39.1 bits (79), Expect(4) = 8e-062
Identities = 17/19 (89%), Positives = 18/19 (94%)
Frame = -2 / -2
Query: 623 NSFLRA*DITYANK*T*HN 567
NSFLR *DITYANK*T*H+
Sbjct: 747 NSFLRV*DITYANK*T*HH 691
Score = 35.9 bits (72), Expect(3) = 3e-079
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +3 / +1
Query: 567 IMLCLFIGISYVLRS*K 617
+MLCLFIGISYVL S*K
Sbjct: 691 MMLCLFIGISYVLHS*K 741
>gb|BG103631.1|BG103631 RHIZ2_38_H11.g1_A003 Rhizome2 (RHIZ2) Sorghum propinquum cDNA, mRNA
sequence
Length = 609
Score = 222 bits (479), Expect = 5e-057
Identities = 75/123 (60%), Positives = 102/123 (82%)
Frame = +2 / +3
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+Y+L +Q++KW DK ++EN YYLHG+W LVD+YEEM+EN+ PGLGD RWP VTHFVGCK
Sbjct: 150 IYLLLSQKDKWMDKVFIENSYYLHGFWAGLVDKYEEMMENHHPGLGDERWPFVTHFVGCK 329
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKDE 361
PCG +GDYPVERCL++M+RAFNF DNQ+L++YGF+HK L S ++KR R++T+ P+ +
Sbjct: 330 PCGSYGDYPVERCLRSMERAFNFADNQVLRLYGFSHKGLESPKIKRTRDQTTRPINDVEN 509
Query: 362 LGL 370
L +
Sbjct: 510 LDM 518
Score = 120 bits (257), Expect = 2e-026
Identities = 58/88 (65%), Positives = 60/88 (68%)
Frame = -1 / -3
Query: 288 CVNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSI 109
C PY WLS KLNARS+ LR RSTG*SP LPHGLHPTK VT GHR SP PG *FS I
Sbjct: 436 CEKPYSRSTWLSAKLNARSMLLRQRSTG*SP*LPHGLHPTK*VTKGHRSSPRPGW*FSII 257
Query: 108 SSYLSTKIPQ*PWR*YPFSR*ALSPHFS 25
SSYLST Q P +* FS LS H S
Sbjct: 256 SSYLSTSPAQKPCK**EFSMNTLSIHLS 173
>gb|CD227098.1|CD227098 CCC1_4_E12.b1_A007 Callus culture/cell suspension Sorghum bicolor
cDNA clone CCC1_4_E12_A007 3', mRNA sequence
Length = 658
Score = 196 bits (422), Expect = 4e-049
Identities = 65/119 (54%), Positives = 95/119 (79%)
Frame = +2 / +1
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+++L Q+E+W DK ++E +YLHG+W LVDRYEEM+E + PGLGD RWP +THFVGCK
Sbjct: 145 IHLLLMQKERWMDKVHVETEFYLHGFWTGLVDRYEEMMEEHHPGLGDDRWPFITHFVGCK 324
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKD 358
CG++ DYP++RC++ M+RAFNF DNQ+L++YGF H+SL + +V+R+ + +NPL K+
Sbjct: 325 TCGRYEDYPLDRCVRGMERAFNFADNQVLRLYGFRHRSLTTAKVRRVTDPATNPLMAKE 501
Score = 107 bits (228), Expect = 2e-022
Identities = 53/92 (57%), Positives = 58/92 (63%)
Frame = -1 / -3
Query: 288 CVNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSI 109
C NPY WLS KL ARS+ L HRS+G*S LP L PTK V +GHR SPSPG S I
Sbjct: 431 CRNPYSRSTWLSAKLKARSMPLTHRSSG*SSYLPQVLQPTKWVMNGHRSSPSPGWCSSII 252
Query: 108 SSYLSTKIPQ*PWR*YPFSR*ALSPHFSRCVA 13
SSYLST Q PWR* S LS H S C++
Sbjct: 251 SSYLSTSPVQNPWR*NSVSTCTLSIHLSFCIS 156
>gb|CW060796.1|CW060796 104_304_10520052_115_30144 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10520052, DNA
sequence
Length = 554
Score = 194 bits (418), Expect = 1e-048
Identities = 65/119 (54%), Positives = 94/119 (78%)
Frame = +2 / -3
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+++L Q+E+W DK ++E +YLHG+W LVDRYEEM+E + PGLGD RWP +THFVGCK
Sbjct: 531 IHLLLMQKERWMDKVHVETEFYLHGFWTGLVDRYEEMMEEHHPGLGDDRWPFITHFVGCK 352
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKD 358
CG++ DYP++RC+ M+RAFNF DNQ+L++YGF H+SL + +V+R+ + +NPL K+
Sbjct: 351 TCGRYEDYPLDRCVGGMERAFNFADNQVLRLYGFRHRSLTTAKVRRVTDPATNPLMAKE 175
Score = 109 bits (232), Expect = 6e-023
Identities = 53/92 (57%), Positives = 58/92 (63%)
Frame = -1 / +2
Query: 288 CVNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSI 109
C NPY WLS KL ARS+ HRS+G*S LP L PTKCV +GHR SPSPG S I
Sbjct: 245 CRNPYSRSTWLSAKLKARSMPPTHRSSG*SSYLPQVLQPTKCVMNGHRSSPSPGWCSSII 424
Query: 108 SSYLSTKIPQ*PWR*YPFSR*ALSPHFSRCVA 13
SSYLST Q PWR* S LS H S C++
Sbjct: 425 SSYLSTSPVQNPWR*NSVSTCTLSIHLSFCIS 520
>gb|CW261852.1|CW261852 104_729_11229737_116_35209_077 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11229737, DNA
sequence
Length = 641
Score = 194 bits (418), Expect = 1e-048
Identities = 65/119 (54%), Positives = 94/119 (78%)
Frame = +2 / -3
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+++L Q+E+W DK ++E +YLHG+W LVDRYEEM+E + PGLGD RWP +THFVGCK
Sbjct: 408 IHLLLMQKERWMDKVHVETEFYLHGFWTGLVDRYEEMMEEHHPGLGDDRWPFITHFVGCK 229
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKD 358
CG++ DYP++RC+ M+RAFNF DNQ+L++YGF H+SL + +V+R+ + +NPL K+
Sbjct: 228 TCGRYEDYPLDRCVGGMERAFNFADNQVLRLYGFRHRSLTTAKVRRVTDPATNPLMAKE 52
Score = 109 bits (232), Expect = 6e-023
Identities = 53/92 (57%), Positives = 58/92 (63%)
Frame = -1 / +2
Query: 288 CVNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSI 109
C NPY WLS KL ARS+ HRS+G*S LP L PTKCV +GHR SPSPG S I
Sbjct: 122 CRNPYSRSTWLSAKLKARSMPPTHRSSG*SSYLPQVLQPTKCVMNGHRSSPSPGWCSSII 301
Query: 108 SSYLSTKIPQ*PWR*YPFSR*ALSPHFSRCVA 13
SSYLST Q PWR* S LS H S C++
Sbjct: 302 SSYLSTSPVQNPWR*NSVSTCTLSIHLSFCIS 397
>gb|CW295416.1|CW295416 104_776_11460918_116_35617_019 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11460918, DNA
sequence
Length = 713
Score = 194 bits (418), Expect = 1e-048
Identities = 65/119 (54%), Positives = 94/119 (78%)
Frame = +2 / -2
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+++L Q+E+W DK ++E +YLHG+W LVDRYEEM+E + PGLGD RWP +THFVGCK
Sbjct: 514 IHLLLMQKERWMDKVHVETEFYLHGFWTGLVDRYEEMMEEHHPGLGDDRWPFITHFVGCK 335
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKD 358
CG++ DYP++RC+ M+RAFNF DNQ+L++YGF H+SL + +V+R+ + +NPL K+
Sbjct: 334 TCGRYEDYPLDRCVGGMERAFNFADNQVLRLYGFRHRSLTTAKVRRVTDPATNPLMAKE 158
Score = 109 bits (232), Expect = 6e-023
Identities = 53/92 (57%), Positives = 58/92 (63%)
Frame = -1 / +3
Query: 288 CVNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSI 109
C NPY WLS KL ARS+ HRS+G*S LP L PTKCV +GHR SPSPG S I
Sbjct: 228 CRNPYSRSTWLSAKLKARSMPPTHRSSG*SSYLPQVLQPTKCVMNGHRSSPSPGWCSSII 407
Query: 108 SSYLSTKIPQ*PWR*YPFSR*ALSPHFSRCVA 13
SSYLST Q PWR* S LS H S C++
Sbjct: 408 SSYLSTSPVQNPWR*NSVSTCTLSIHLSFCIS 503
>gb|CN130358.1|CN130358 RHOH1_41_A07.b1_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_41_A07_A002 3', mRNA sequence
Length = 729
Score = 194 bits (418), Expect = 1e-048
Identities = 65/119 (54%), Positives = 94/119 (78%)
Frame = +2 / +2
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+++L Q+E+W DK ++E +YLHG+W LVDRYEEM+E + PGLGD RWP +THFVGCK
Sbjct: 143 IHLLLMQKERWMDKVHVETEFYLHGFWTGLVDRYEEMMEEHHPGLGDDRWPFITHFVGCK 322
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKD 358
CG++ DYP++RC+ M+RAFNF DNQ+L++YGF H+SL + +V+R+ + +NPL K+
Sbjct: 323 TCGRYEDYPLDRCVGGMERAFNFADNQVLRLYGFRHRSLTTAKVRRVTDPATNPLMAKE 499
Score = 109 bits (232), Expect = 6e-023
Identities = 53/92 (57%), Positives = 58/92 (63%)
Frame = -1 / -1
Query: 288 CVNPYICKIWLSPKLNARSIFLRHRSTG*SPNLPHGLHPTKCVTSGHR*SPSPGL*FSSI 109
C NPY WLS KL ARS+ HRS+G*S LP L PTKCV +GHR SPSPG S I
Sbjct: 429 CRNPYSRSTWLSAKLKARSMPPTHRSSG*SSYLPQVLQPTKCVMNGHRSSPSPGWCSSII 250
Query: 108 SSYLSTKIPQ*PWR*YPFSR*ALSPHFSRCVA 13
SSYLST Q PWR* S LS H S C++
Sbjct: 249 SSYLSTSPVQNPWR*NSVSTCTLSIHLSFCIS 154
>gb|CW444261.1|CW444261 fsbb001f164m08k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f164m08, DNA
sequence
Length = 642
Score = 172 bits (370), Expect = 6e-042
Identities = 63/64 (98%), Positives = 63/64 (98%)
Frame = +2 / -2
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
VYILATQREKWGDK YLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK
Sbjct: 224 VYILATQREKWGDKVYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 45
Query: 182 PCGK 193
PCGK
Sbjct: 44 PCGK 33
Score = 149 bits (319), Expect = 6e-035
Identities = 63/65 (96%), Positives = 63/65 (96%)
Frame = -1 / +1
Query: 195 NLPHGLHPTKCVTSGHR*SPSPGL*FSSISSYLSTKIPQ*PWR*YPFSR*ALSPHFSRCV 16
NLPHGLHPTKCVTSGHR*SPSPGL*FSSISSYLSTKIPQ*P R*YPFSR* LSPHFSRCV
Sbjct: 31 NLPHGLHPTKCVTSGHR*SPSPGL*FSSISSYLSTKIPQ*P*R*YPFSR*TLSPHFSRCV 210
Query: 15 ARMYT 1
ARMYT
Sbjct: 211 ARMYT 225
Score = 133 bits (284), Expect = 4e-030
Identities = 58/69 (84%), Positives = 61/69 (88%)
Frame = +1 / -1
Query: 1 GVHPRDTAREMG**GLP*EWVLPPRLLGDLGRQVRGDA*ELQARARRSPVATRHTLCRVQ 180
GVHPR+ ARE+G**GLP*EWVLP LLGDLGRQV GDA*ELQARARRSPVA RHTLCRVQ
Sbjct: 225 GVHPRNAAREVG**GLP*EWVLPSWLLGDLGRQV*GDA*ELQARARRSPVAARHTLCRVQ 46
Query: 181 AVWQIWRLP 207
A+WQI P
Sbjct: 45 AMWQIGDYP 19
Score = 132 bits (283), Expect = 6e-030
Identities = 55/65 (84%), Positives = 60/65 (92%)
Frame = -3 / +3
Query: 196 KFATRLAPDKVCDEWPPVIAEPWLVILKHLLVPVDQDPPVAVEVVPILKVSLITPFLALC 17
+FAT LAPDKVCDE PPVIAEPWLVILKHLL+PVDQDPPVA++VVPILKV+LITP LAL
Sbjct: 30 QFATWLAPDKVCDERPPVIAEPWLVILKHLLIPVDQDPPVAMKVVPILKVNLITPLLALR 209
Query: 16 REDVH 2
EDVH
Sbjct: 210 CEDVH 224
>gb|CW216402.1|CW216402 104_649_11195097_148_37462_043 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11195097, DNA
sequence
Length = 552
Score = 103 bits (219), Expect(2) = 1e-041
Identities = 35/62 (56%), Positives = 46/62 (74%)
Frame = +2 / -3
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
V++L T++E+W D+ YLEN +YLHG W LV ++EE +E + PG GD RWP VTHF GCK
Sbjct: 445 VHLLITEKERWMDRVYLENQFYLHGVWTGLVGKFEEAMEKHHPGYGDDRWPFVTHFAGCK 266
Query: 182 PC 187
C
Sbjct: 265 IC 260
Score = 88.6 bits (187), Expect(2) = 1e-041
Identities = 30/54 (55%), Positives = 45/54 (83%)
Frame = +2 / -3
Query: 200 DYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKDE 361
+YP++RC+ M+RAFNF DNQ+L++YGF H+SLA+ V+R+ N ++NPLE K+E
Sbjct: 196 EYPLDRCVGGMERAFNFADNQVLRLYGFRHQSLATAEVRRVANRSANPLEAKEE 35
>gb|CW069905.1|CW069905 104_320_10526223_1_30089 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10526223, DNA
sequence
Length = 550
Score = 103 bits (219), Expect(2) = 1e-041
Identities = 35/62 (56%), Positives = 46/62 (74%)
Frame = +2 / -1
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
V++L T++E+W D+ YLEN +YLHG W LV ++EE +E + PG GD RWP VTHF GCK
Sbjct: 547 VHLLITEKERWMDRVYLENQFYLHGVWTGLVGKFEEAMEKHHPGYGDDRWPFVTHFAGCK 368
Query: 182 PC 187
C
Sbjct: 367 IC 362
Score = 88.6 bits (187), Expect(2) = 1e-041
Identities = 30/54 (55%), Positives = 45/54 (83%)
Frame = +2 / -1
Query: 200 DYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKDE 361
+YP++RC+ M+RAFNF DNQ+L++YGF H+SLA+ V+R+ N ++NPLE K+E
Sbjct: 298 EYPLDRCVGGMERAFNFADNQVLRLYGFRHQSLATAEVRRVANRSANPLEAKEE 137
>gb|CW217099.1|CW217099 104_650_11195476_148_37131_012 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11195476, DNA
sequence
Length = 586
Score = 100 bits (213), Expect(2) = 6e-040
Identities = 34/62 (54%), Positives = 45/62 (72%)
Frame = +2 / -2
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
V++L T++E+W D+ YLEN +YLHG W LV ++EE +E + PG GD RWP VTH GCK
Sbjct: 492 VHLLITEKERWMDRVYLENQFYLHGVWTGLVGKFEEAMEKHHPGYGDDRWPFVTHLAGCK 313
Query: 182 PC 187
C
Sbjct: 312 IC 307
Score = 85.4 bits (180), Expect(2) = 6e-040
Identities = 29/54 (53%), Positives = 44/54 (81%)
Frame = +2 / -2
Query: 200 DYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKDE 361
+YP++RC+ M+RAFNF DNQ+L++YG H+SLA+ V+R+ N ++NPLE K+E
Sbjct: 243 EYPLDRCVGGMERAFNFADNQVLRLYGVRHQSLATAEVRRVANRSANPLEAKEE 82
>gb|CW485924.1|CW485924 fsbb001f248o11k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f248o11, DNA
sequence
Length = 551
Score = 159 bits (342), Expect = 4e-038
Identities = 53/90 (58%), Positives = 73/90 (81%)
Frame = +2 / -3
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+++L Q+E+W DK ++E +YLHG+W LVDRYEEM+E + PGLGD RWP +THFVGCK
Sbjct: 273 IHLLLMQKERWMDKVHVETEFYLHGFWTGLVDRYEEMMEEHHPGLGDDRWPFITHFVGCK 94
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQ 271
CG++ DYP++RC+ M+RAFNF DNQ+L+
Sbjct: 93 TCGRYEDYPLDRCVGGMERAFNFADNQVLR 4
>gb|CW295417.1|CW295417 104_776_11460918_148_35613_019 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11460918, DNA
sequence
Length = 621
Score = 157 bits (338), Expect = 1e-037
Identities = 53/91 (58%), Positives = 73/91 (80%)
Frame = +2 / +2
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+++L Q+E+W DK ++E +YLHG+W LVDRYEEM+E + PGLGD RWP +THFVGCK
Sbjct: 347 IHLLLMQKERWMDKVHVETEFYLHGFWTGLVDRYEEMMEEHHPGLGDDRWPFITHFVGCK 526
Query: 182 PCGKFGDYPVERCLKNMDRAFNFGDNQILQM 274
CG++ DYP++RC+ M RAFNF DNQ+L++
Sbjct: 527 TCGRYEDYPLDRCVGGM*RAFNFADNQVLRL 619
>gb|CW227932.1|CW227932 104_667_11206082_116_37242_005 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11206082, DNA
sequence
Length = 698
Score = 74.8 bits (157), Expect(3) = 3e-033
Identities = 28/58 (48%), Positives = 39/58 (67%)
Frame = +2 / -3
Query: 188 GKFGDYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLASRRVKRIRNETSNPLETKDE 361
G Y C ++M+RA NFGD+QIL +YGF HK+L + V+R+RN+T PL+ DE
Sbjct: 294 GDDSTYDAAWCRRSMERALNFGDDQILNLYGFEHKTLNTTAVRRVRNDTGGPLDAADE 121
Score = 59.7 bits (124), Expect(3) = 3e-033
Identities = 20/37 (54%), Positives = 28/37 (75%)
Frame = +2 / -3
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEM 112
VY+L T+R +WGDK +LE+ Y L G+W +VD+ EEM
Sbjct: 495 VYLLVTERRRWGDKTFLESSYCLSGFWVYIVDKLEEM 385
Score = 46.9 bits (96), Expect(3) = 3e-033
Identities = 15/21 (71%), Positives = 16/21 (76%)
Frame = +2 / -3
Query: 128 PGLGDHRWPLVTHFVGCKPCG 190
P G RWPL THF+GCKPCG
Sbjct: 357 PEPGHERWPLTTHFMGCKPCG 295
>gb|CW217098.1|CW217098 104_650_11195476_116_37132_012 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11195476, DNA
sequence
Length = 610
Score = 103 bits (219), Expect(2) = 1e-031
Identities = 35/62 (56%), Positives = 46/62 (74%)
Frame = +2 / +1
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
V++L T++E+W D+ YLEN +YLHG W LV ++EE +E + PG GD RWP VTHF GCK
Sbjct: 256 VHLLITEKERWMDRVYLENQFYLHGVWTGLVGKFEEAMEKHHPGYGDDRWPFVTHFAGCK 435
Query: 182 PC 187
C
Sbjct: 436 IC 441
Score = 54.7 bits (113), Expect(2) = 1e-031
Identities = 18/35 (51%), Positives = 28/35 (80%)
Frame = +2 / +1
Query: 200 DYPVERCLKNMDRAFNFGDNQILQMYGFTHKSLAS 304
+Y ++RC+ M+RAFNF D +L++YGF H+SLA+
Sbjct: 505 EYSLDRCVGGMERAFNFADEPVLRLYGFRHQSLAT 609
>gb|CW038350.1|CW038350 104_270_10504872_115_30386 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10504872, DNA
sequence
Length = 625
Score = 131 bits (280), Expect = 1e-029
Identities = 43/63 (68%), Positives = 54/63 (85%)
Frame = +2 / -2
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
+Y+L +Q++KW DK ++EN YYLHG+W LVD+YEEM+EN+ PGLGD RWP VTHFVGCK
Sbjct: 192 IYLLLSQKDKWMDKVFIENSYYLHGFWAGLVDKYEEMMENHHPGLGDERWPFVTHFVGCK 13
Query: 182 PCG 190
PCG
Sbjct: 12 PCG 4
>gb|CW058700.1|CW058700 104_301_10518886_114_30160 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10518886, DNA
sequence
Length = 550
Score = 103 bits (219), Expect = 4e-021
Identities = 35/62 (56%), Positives = 46/62 (74%)
Frame = +2 / -2
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
V++L T++E+W D+ YLEN +YLHG W LV ++EE +E + PG GD RWP VTHF GCK
Sbjct: 186 VHLLITEKERWMDRVYLENQFYLHGVWTGLVGKFEEAMEKHHPGYGDDRWPFVTHFAGCK 7
Query: 182 PC 187
C
Sbjct: 6 IC 1
>gb|CW059684.1|CW059684 104_302_10519462_114_30165 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10519462, DNA
sequence
Length = 574
Score = 103 bits (219), Expect = 4e-021
Identities = 35/62 (56%), Positives = 46/62 (74%)
Frame = +2 / -2
Query: 2 VYILATQREKWGDKAYLENGYYLHGYWGILVDRYEEMLENYKPGLGDHRWPLVTHFVGCK 181
V++L T++E+W D+ YLEN +YLHG W LV ++EE +E + PG GD RWP VTHF GCK
Sbjct: 186 VHLLITEKERWMDRVYLENQFYLHGVWTGLVGKFEEAMEKHHPGYGDDRWPFVTHFAGCK 7
Query: 182 PC 187
C
Sbjct: 6 IC 1
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 947,578,723
Number of Sequences: 832831
Number of extensions: 13833897
Number of successful extensions: 77020
Number of sequences better than 1.0e-020: 19
length of database: 163,786,556
effective HSP length: 54
effective length of database: 118,813,682
effective search space used: 23287481672
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)