TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 1805139.2.3
         (438 letters)

Database: Sorghum_nucl_with_EST.fasta 
           832,831 sequences; 491,359,669 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AW671472.1|AW671472  LG1_347_C12.b1_A002 Light Grown 1 (L...    82   4e-031
gb|CW432390.1|CW432390  fsbb001f147b04k0 Sorghum methylation...   104   7e-022

>gb|AW671472.1|AW671472 LG1_347_C12.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA
           sequence
          Length = 674

 Score = 81.7 bits (172), Expect(2) = 4e-031
 Identities = 29/30 (96%), Positives = 30/30 (100%)
 Frame = -3 / +1

                                         
Query: 106 QVTVNNTMYHKSCFKCCHGGCTISPSNYIA 17
           +VTVNNTMYHKSCFKCCHGGCTISPSNYIA
Sbjct: 583 KVTVNNTMYHKSCFKCCHGGCTISPSNYIA 672




 Score = 73.5 bits (154), Expect(2) = 4e-031
 Identities = 28/29 (96%), Positives = 28/29 (96%)
 Frame = -2 / +3

                                        
Query: 269 ECY*SLKRLCWHQREMCWMQQDSLSN*EG 183
           EC *SLKRLCWHQREMCWMQQDSLSN*EG
Sbjct: 501 ECC*SLKRLCWHQREMCWMQQDSLSN*EG 587




 Score = 72.1 bits (151), Expect(2) = 3e-026
 Identities = 26/30 (86%), Positives = 28/30 (93%)
 Frame = -2 / +3

                                         
Query: 104 GHC*QHYVPQELLQVLPWRVHHQPF*LHCA 15
           G+C QHYV QELLQVLPWR+HHQPF*LHCA
Sbjct: 585 GYCQQHYVSQELLQVLPWRMHHQPF*LHCA 674




 Score = 71.6 bits (150), Expect(2) = 2e-025
 Identities = 29/33 (87%), Positives = 31/33 (93%)
 Frame = +3 / -1

                                            
Query: 15  CAM*LEGLMVHPPWQHLKQLLWYIVLLTVTWML 113
           CAM*LEGLMVHPPWQHLKQLL YIVLLTVT+ +
Sbjct: 674 CAM*LEGLMVHPPWQHLKQLL*YIVLLTVTFSI 576




 Score = 66.6 bits (139), Expect(2) = 3e-026
 Identities = 26/30 (86%), Positives = 28/30 (93%)
 Frame = -3 / +1

                                         
Query: 271 QNATKVSSAFAGTREKCVGCSKTVYPTERV 182
           +NA KVSSAFAGTREKCVGCSKTVYP E+V
Sbjct: 499 ENAVKVSSAFAGTREKCVGCSKTVYPIEKV 588




 Score = 64.3 bits (134), Expect(2) = 2e-025
 Identities = 28/36 (77%), Positives = 32/36 (88%)
 Frame = +2 / -3

                                               
Query: 176 ESYPLSWIDCLAASNTFLSGASKGA*DFSSILPNNM 283
           +S  L+WIDCLAASNTFLSGASKGA*DF+SIL  N+
Sbjct: 594 DSNLLNWIDCLAASNTFLSGASKGA*DFNSILIPNV 487




 Score = 62.9 bits (131), Expect(2) = 2e-021
 Identities = 26/30 (86%), Positives = 28/30 (93%)
 Frame = +3 / -1

                                         
Query: 180 LTLSVG*TVLLHPTHFSLVPAKALETLVAF 269
           +T S+G*TVLLHPTHFSLVPAKALETL AF
Sbjct: 590 VTFSIG*TVLLHPTHFSLVPAKALETLTAF 501




 Score = 59.3 bits (123), Expect(2) = 2e-021
 Identities = 26/30 (86%), Positives = 29/30 (96%)
 Frame = +2 / -3

                                         
Query: 17  RNVVRRADGAPSMAALEAALVVHSVVNSDL 106
           RNVVRRADGA SMAALEAALV+HSVV+S+L
Sbjct: 672 RNVVRRADGASSMAALEAALVIHSVVDSNL 583



>gb|CW432390.1|CW432390 fsbb001f147b04k0 Sorghum methylation filtered library (LibID: 104)
           Sorghum bicolor genomic clone fsbb001f147b04, DNA
           sequence
          Length = 564

 Score =  104 bits (222), Expect = 7e-022
 Identities = 39/40 (97%), Positives = 40/40 (100%)
 Frame = -3 / -3

                                                   
Query: 121 NRLSIQVTVNNTMYHKSCFKCCHGGCTISPSNYIAHEGKL 2
           +RLSIQVTVNNTMYHKSCFKCCHGGCTISPSNYIAHEGKL
Sbjct: 325 HRLSIQVTVNNTMYHKSCFKCCHGGCTISPSNYIAHEGKL 206




 Score = 94.5 bits (200), Expect(2) = 3e-021
 Identities = 38/39 (97%), Positives = 38/39 (97%)
 Frame = +3 / +3

                                                  
Query: 3   SFPSCAM*LEGLMVHPPWQHLKQLLWYIVLLTVTWMLNR 119
           SFPSCAM*LEGLMVHPPWQHLKQLL YIVLLTVTWMLNR
Sbjct: 207 SFPSCAM*LEGLMVHPPWQHLKQLL*YIVLLTVTWMLNR 323




 Score = 28.1 bits (55), Expect(2) = 3e-021
 Identities = 10/12 (83%), Positives = 11/12 (91%)
 Frame = +1 / +1

                       
Query: 112 LTGCQVKKAQTT 147
           +TGCQVKK QTT
Sbjct: 529 VTGCQVKKTQTT 564



  Database: Sorghum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:54 PM
  Number of letters in database: 491,359,669
  Number of sequences in database:  832,831
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 525,568,488
Number of Sequences: 832831
Number of extensions: 6692708
Number of successful extensions: 119281
Number of sequences better than 1.0e-020: 2
length of database: 163,786,556
effective HSP length: 52
effective length of database: 120,479,344
effective search space used: 11204578992
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)