TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131543.2.1
(634 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ655932.1|BQ655932 pRC0111 Sorghum halepense rhizome cD... 134 1e-030
gb|AI724675.1|AI724675 RHIZ1_11_F01.y1_A001 Rhizome1 (RHIZ1... 133 2e-030
gb|AW287208.2|AW287208 LG1_267_H06.b1_A002 Light Grown 1 (L... 133 2e-030
gb|BE352866.1|BE352866 LG1_267_H06.g1_A002 Light Grown 1 (L... 133 2e-030
gb|BG049368.1|BG049368 OV1_19_B01.g1_A002 Ovary 1 (OV1) Sor... 133 2e-030
gb|CF760799.1|CF760799 DSAF1_66_H03.b1_A011 Drought-stresse... 133 2e-030
gb|CF772774.1|CF772774 DSBF1_39_C05.g1_A010 Drought-stresse... 133 2e-030
gb|BG240197.1|BG240197 OV1_19_B01.b1_A002 Ovary 1 (OV1) Sor... 132 6e-030
>gb|BQ655932.1|BQ655932 pRC0111 Sorghum halepense rhizome cDNA library Sorghum halepense
cDNA clone pRC0111, mRNA sequence
Length = 371
Score = 134 bits (287), Expect = 1e-030
Identities = 54/57 (94%), Positives = 55/57 (96%)
Frame = +3 / -2
Query: 120 PEEMAKSKNHTAHNQSFKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 290
P MAKSKNHTAHNQS+KAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG
Sbjct: 370 PLWMAKSKNHTAHNQSYKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 200
Score = 119 bits (254), Expect(2) = 4e-030
Identities = 45/49 (91%), Positives = 46/49 (93%)
Frame = -1 / +3
Query: 265 EYLRFLRNLGSIPLVEVWRWRLGFLMPFLCALNDWLCAVWFFDLAISSG 119
EYL+FLRNLGSIPLVEVWRWRLGFLMPFLCAL DWLCAVWFFDLAI G
Sbjct: 225 EYLKFLRNLGSIPLVEVWRWRLGFLMPFLCALYDWLCAVWFFDLAIQRG 371
Score = 33.1 bits (66), Expect(2) = 4e-030
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -2 / +1
Query: 309 TPQLQPHHSFCCPS 268
TP QPHHS CCPS
Sbjct: 181 TPLPQPHHSSCCPS 222
>gb|AI724675.1|AI724675 RHIZ1_11_F01.y1_A001 Rhizome1 (RHIZ1) Sorghum halepense cDNA, mRNA
sequence
Length = 472
Score = 133 bits (286), Expect = 2e-030
Identities = 53/54 (98%), Positives = 54/54 (100%)
Frame = +3 / +2
Query: 129 MAKSKNHTAHNQSFKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 290
MAKSKNHTAHNQS+KAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG
Sbjct: 80 MAKSKNHTAHNQSYKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 241
Score = 115 bits (245), Expect(2) = 9e-028
Identities = 43/46 (93%), Positives = 44/46 (95%)
Frame = -1 / -2
Query: 265 EYLRFLRNLGSIPLVEVWRWRLGFLMPFLCALNDWLCAVWFFDLAI 128
EYL+FLRN GSIPLVEVWRWRLGFLMPFLCAL DWLCAVWFFDLAI
Sbjct: 216 EYLKFLRNFGSIPLVEVWRWRLGFLMPFLCALYDWLCAVWFFDLAI 79
Score = 29.5 bits (58), Expect(2) = 9e-028
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -2 / -3
Query: 309 TPQLQPHHSFCCPS 268
TP QPH S CCPS
Sbjct: 260 TPLPQPHRSSCCPS 219
>gb|AW287208.2|AW287208 LG1_267_H06.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 429
Score = 133 bits (286), Expect = 2e-030
Identities = 53/54 (98%), Positives = 54/54 (100%)
Frame = +3 / +1
Query: 129 MAKSKNHTAHNQSFKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 290
MAKSKNHTAHNQS+KAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG
Sbjct: 70 MAKSKNHTAHNQSYKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 231
Score = 116 bits (249), Expect(2) = 3e-028
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = -1 / -2
Query: 265 EYLRFLRNLGSIPLVEVWRWRLGFLMPFLCALNDWLCAVWFFDLAI 128
EYL+FLRNLGSIPLVEVWRWRLGFLMPFLCAL DWLCAVWFFDLAI
Sbjct: 206 EYLKFLRNLGSIPLVEVWRWRLGFLMPFLCALYDWLCAVWFFDLAI 69
Score = 29.5 bits (58), Expect(2) = 3e-028
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -2 / -3
Query: 309 TPQLQPHHSFCCPS 268
TP Q HHS CCPS
Sbjct: 250 TPLPQAHHSSCCPS 209
>gb|BE352866.1|BE352866 LG1_267_H06.g1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA
sequence
Length = 419
Score = 133 bits (286), Expect = 2e-030
Identities = 53/54 (98%), Positives = 54/54 (100%)
Frame = +3 / +2
Query: 129 MAKSKNHTAHNQSFKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 290
MAKSKNHTAHNQS+KAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG
Sbjct: 56 MAKSKNHTAHNQSYKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 217
Score = 116 bits (249), Expect(2) = 3e-028
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = -1 / -3
Query: 265 EYLRFLRNLGSIPLVEVWRWRLGFLMPFLCALNDWLCAVWFFDLAI 128
EYL+FLRNLGSIPLVEVWRWRLGFLMPFLCAL DWLCAVWFFDLAI
Sbjct: 192 EYLKFLRNLGSIPLVEVWRWRLGFLMPFLCALYDWLCAVWFFDLAI 55
Score = 29.5 bits (58), Expect(2) = 3e-028
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -2 / -1
Query: 309 TPQLQPHHSFCCPS 268
TP Q HHS CCPS
Sbjct: 236 TPLPQAHHSSCCPS 195
>gb|BG049368.1|BG049368 OV1_19_B01.g1_A002 Ovary 1 (OV1) Sorghum bicolor cDNA, mRNA
sequence
Length = 352
Score = 133 bits (286), Expect = 2e-030
Identities = 53/54 (98%), Positives = 54/54 (100%)
Frame = +3 / +1
Query: 129 MAKSKNHTAHNQSFKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 290
MAKSKNHTAHNQS+KAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG
Sbjct: 85 MAKSKNHTAHNQSYKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 246
Score = 115 bits (245), Expect(2) = 6e-029
Identities = 43/46 (93%), Positives = 44/46 (95%)
Frame = -1 / -3
Query: 265 EYLRFLRNLGSIPLVEVWRWRLGFLMPFLCALNDWLCAVWFFDLAI 128
EYL+FLRN GSIPLVEVWRWRLGFLMPFLCAL DWLCAVWFFDLAI
Sbjct: 221 EYLKFLRNFGSIPLVEVWRWRLGFLMPFLCALYDWLCAVWFFDLAI 84
Score = 33.1 bits (66), Expect(2) = 6e-029
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -2 / -1
Query: 309 TPQLQPHHSFCCPS 268
TP QPHHS CCPS
Sbjct: 265 TPLPQPHHSSCCPS 224
>gb|CF760799.1|CF760799 DSAF1_66_H03.b1_A011 Drought-stressed after flowering Sorghum
bicolor cDNA clone DSAF1_66_H03_A011 5', mRNA sequence
Length = 448
Score = 133 bits (286), Expect = 2e-030
Identities = 53/54 (98%), Positives = 54/54 (100%)
Frame = +3 / +1
Query: 129 MAKSKNHTAHNQSFKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 290
MAKSKNHTAHNQS+KAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG
Sbjct: 64 MAKSKNHTAHNQSYKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 225
Score = 116 bits (249), Expect(2) = 2e-029
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = -1 / -3
Query: 265 EYLRFLRNLGSIPLVEVWRWRLGFLMPFLCALNDWLCAVWFFDLAI 128
EYL+FLRNLGSIPLVEVWRWRLGFLMPFLCAL DWLCAVWFFDLAI
Sbjct: 200 EYLKFLRNLGSIPLVEVWRWRLGFLMPFLCALYDWLCAVWFFDLAI 63
Score = 33.1 bits (66), Expect(2) = 2e-029
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -2 / -1
Query: 309 TPQLQPHHSFCCPS 268
TP QPHHS CCPS
Sbjct: 244 TPLPQPHHSSCCPS 203
>gb|CF772774.1|CF772774 DSBF1_39_C05.g1_A010 Drought-stressed before flowering Sorghum
bicolor cDNA clone DSBF1_39_C05_A010 3', mRNA sequence
Length = 426
Score = 133 bits (286), Expect = 2e-030
Identities = 53/54 (98%), Positives = 54/54 (100%)
Frame = +3 / +1
Query: 129 MAKSKNHTAHNQSFKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 290
MAKSKNHTAHNQS+KAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG
Sbjct: 64 MAKSKNHTAHNQSYKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 225
Score = 116 bits (249), Expect(2) = 2e-029
Identities = 44/46 (95%), Positives = 45/46 (97%)
Frame = -1 / -2
Query: 265 EYLRFLRNLGSIPLVEVWRWRLGFLMPFLCALNDWLCAVWFFDLAI 128
EYL+FLRNLGSIPLVEVWRWRLGFLMPFLCAL DWLCAVWFFDLAI
Sbjct: 200 EYLKFLRNLGSIPLVEVWRWRLGFLMPFLCALYDWLCAVWFFDLAI 63
Score = 33.1 bits (66), Expect(2) = 2e-029
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -2 / -3
Query: 309 TPQLQPHHSFCCPS 268
TP QPHHS CCPS
Sbjct: 244 TPLPQPHHSSCCPS 203
>gb|BG240197.1|BG240197 OV1_19_B01.b1_A002 Ovary 1 (OV1) Sorghum bicolor cDNA, mRNA
sequence
Length = 412
Score = 132 bits (282), Expect = 6e-030
Identities = 52/54 (96%), Positives = 53/54 (98%)
Frame = +3 / +1
Query: 129 MAKSKNHTAHNQSFKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 290
MAKSKNHT HNQS+KAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG
Sbjct: 85 MAKSKNHTVHNQSYKAHKNGIKKPKRHRQTSTKGMDPKFLRNLRYSRKGNKKSG 246
Score = 113 bits (241), Expect(2) = 3e-028
Identities = 42/46 (91%), Positives = 43/46 (93%)
Frame = -1 / -3
Query: 265 EYLRFLRNLGSIPLVEVWRWRLGFLMPFLCALNDWLCAVWFFDLAI 128
EYL+FLRN GSIPLVEVWRWRLGFLMPFLCAL DWLC VWFFDLAI
Sbjct: 221 EYLKFLRNFGSIPLVEVWRWRLGFLMPFLCALYDWLCTVWFFDLAI 84
Score = 33.1 bits (66), Expect(2) = 3e-028
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -2 / -1
Query: 309 TPQLQPHHSFCCPS 268
TP QPHHS CCPS
Sbjct: 265 TPLPQPHHSSCCPS 224
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 638,504,989
Number of Sequences: 832831
Number of extensions: 7114978
Number of successful extensions: 94869
Number of sequences better than 1.0e-020: 8
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 18784502541
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)