TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS19f07.yg.2.1
(626 letters)
Database: Populus_nucl_with_EST.fasta
369,679 sequences; 203,408,664 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX177283.1|CX177283 B04_69-106_04.ab1 leaf inoculated wi... 208 9e-059
gb|CX171881.1|CX171881 A07_69-49_01.ab1 leaf inoculated wit... 141 5e-050
gb|DT475076.1|DT475076 WS0125.BR_O23 PT-GT-FL-A-3 Populus t... 149 4e-047
gb|DT488034.1|DT488034 WS02535.B21_F07 PT-MB-N-A-15 Populus... 147 5e-035
gb|CN519772.1|CN519772 GQ0106.B3_E05 GQ010 Populus trichoca... 146 1e-034
gb|DN496803.1|DN496803 Q019A06.5pR Populus dormant bud cDNA... 112 5e-026
gb|BI138876.1|BI138876 F118P18Y Populus flower cDNA library... 75 2e-025
gb|DT491850.1|DT491850 WS02548.C21_P18 PT-MB-N-A-15 Populus... 115 2e-025
gb|BU870838.1|BU870838 Q019A06 Populus flower cDNA library ... 112 4e-025
gb|CA930279.1|CA930279 MTU2CA.P9.E10 Aspen apex cDNA Librar... 75 1e-022
gb|DT482117.1|DT482117 WS02533.BR_P08 PT-MB-N-A-15 Populus ... 105 3e-022
>gb|CX177283.1|CX177283 B04_69-106_04.ab1 leaf inoculated with Marssonia pathogen of
Populus deltoides Populus deltoides cDNA, mRNA sequence
Length = 588
Score = 208 bits (449), Expect(2) = 9e-059
Identities = 78/96 (81%), Positives = 86/96 (89%)
Frame = -1 / +3
Query: 461 QVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTR 282
Q+SLGMRFRM+FETE+SG RRYMGTITGI DLDP+RWKNS WRNLQVGWDESTA ERR R
Sbjct: 12 QISLGMRFRMMFETEESGTRRYMGTITGISDLDPVRWKNSQWRNLQVGWDESTAGERRNR 191
Query: 281 VSIWEIEPVATPFYICPPPFFRPKLPKQAGMPDDEN 174
VSIWEIEPV PF+ICPPPFFR K P+Q GMPDD++
Sbjct: 192 VSIWEIEPVTAPFFICPPPFFRSKRPRQPGMPDDDS 299
Score = 153 bits (329), Expect(2) = 4e-041
Identities = 67/104 (64%), Positives = 78/104 (75%)
Frame = +1 / -3
Query: 145 IALLKALSTSFSSSGIPACLGSFGLKNGGGHI*NGVATGSISHIETRVRLSDAVDSSQPT 324
+ L K + SSSGIP CLG LKNGGGHI NG TGSISHIETR+RLS AVDSSQPT
Sbjct: 328 VLLNKLSKSEESSSGIPGCLGRLDLKNGGGHIKNGAVTGSISHIETRLRLSPAVDSSQPT 149
Query: 325 *RFRQ*EFFHRSGSKSPMPVIVPMYLLTPESSVSKSILNRIPRD 456
+ R EFFH +GS+S +PV+VP+YLL P+SSVS + RIPRD
Sbjct: 148 CKLRHCEFFHLTGSRSLIPVMVPIYLLVPDSSVSNIMRKRIPRD 17
Score = 100 bits (212), Expect(2) = 4e-022
Identities = 43/96 (44%), Positives = 61/96 (63%)
Frame = +3 / -2
Query: 180 IIWHSCLLRKLWPEKWWWAYIKWCSNWFNLPYRNTGAPLRCSRFIPTNLKVSPMRILPSQ 359
IIWHS L L ++W Y K SNWFNLPY ++ A S FIPTNL+++P+ I PS
Sbjct: 293 IIWHSWLPWTLRSKEWRRTYKKRSSNWFNLPY*DSIASFTSS*FIPTNLQITPL*IFPSY 114
Query: 360 WI*VANACDRAHVSSYP*ILCLKEHSESHSKGHLCI 467
I + + C+ AH+ S *+ K H+++HSKG+L +
Sbjct: 113 RIKITDTCNGAHIPSCS*LFSFKHHAKAHSKGYLVL 6
Score = 77.1 bits (162), Expect(2) = 7e-022
Identities = 33/79 (41%), Positives = 54/79 (68%)
Frame = -3 / +2
Query: 459 SVPWNAIQNAL*DRGFRGKKIHGHDHRHWRLRSTAMEEFSLAKPSGWLG*IDCI*EAHPC 280
++PWNA+ + +*+ R KK++GH +R+ S ME+F++A +GWLG*I+C *+
Sbjct: 14 NIPWNALSHDV*N*RVRNKKVYGHHYRYQ*S*SCKMEKFTMA*FAGWLG*INCW*KTQSS 193
Query: 279 FDMGD*TSCYTILYMPTTI 223
+MGD*TS ++ YM ++I
Sbjct: 194 LNMGD*TSYCSVFYMSSSI 250
Score = 46.4 bits (95), Expect(2) = 7e-022
Identities = 19/47 (40%), Positives = 26/47 (55%)
Frame = -1 / +3
Query: 245 FYICPPPFFRPKLPKQAGMPDDENEVESAFKRAMPWLADDFALKDVQ 105
F PP F + + DD ++ +S FKR MPWL D+F +KD Q
Sbjct: 231 FICPPPFFRSKRPRQPGMPDDDSSDFDSLFKRTMPWLGDEFCMKDPQ 371
Score = 38.6 bits (78), Expect(2) = 9e-059
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -1 / +3
Query: 92 PGLSLVQWMAMQQNPQM 42
PGLSLVQWM MQQNP +
Sbjct: 378 PGLSLVQWMNMQQNPSL 428
Score = 34.5 bits (69), Expect(2) = 4e-041
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = +1 / -3
Query: 49 GFCCIAIH*TRLRPG 93
GFCCI IH T+LRPG
Sbjct: 421 GFCCIFIHWTKLRPG 377
Score = 24.4 bits (47), Expect(2) = 4e-022
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = +3 / -2
Query: 48 RILLHSHPLN*A 83
R+LLH HPL+*A
Sbjct: 422 RVLLHIHPLD*A 387
>gb|CX171881.1|CX171881 A07_69-49_01.ab1 leaf inoculated with Marssonia pathogen of Populus
deltoides Populus deltoides cDNA, mRNA sequence
Length = 756
Score = 141 bits (303), Expect(2) = 5e-050
Identities = 55/73 (75%), Positives = 64/73 (87%)
Frame = -1 / +2
Query: 467 YTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERR 288
YT+VS+GMRFRMLFETE+S VRRYMGTITGI DLD RW NS WR+++VGWDESTA ER+
Sbjct: 281 YTRVSVGMRFRMLFETEESSVRRYMGTITGISDLDVARWPNSLWRSVKVGWDESTAGERQ 460
Query: 287 TRVSIWEIEPVAT 249
RVS+WEIEP+ T
Sbjct: 461 PRVSLWEIEPLTT 499
Score = 95.5 bits (202), Expect(2) = 7e-025
Identities = 51/91 (56%), Positives = 56/91 (61%)
Frame = +1 / -1
Query: 193 PACLGSFGLKNGGGHI*NGVATGSISHIETRVRLSDAVDSSQPT*RFRQ*EFFHRSGSKS 372
P G L+ G V +GSISH ET LS AVDSSQPT RQ EF H + SKS
Sbjct: 555 PGVHGRLSLRGNGDGYIGKVVSGSISHRETLGCLSPAVDSSQPTLTERQSEFGHLATSKS 376
Query: 373 PMPVIVPMYLLTPESSVSKSILNRIPRDTCV 465
MPVIVPMY T +SSVS SIL R+P DT V
Sbjct: 375 LMPVIVPMYRRTLDSSVSNSILKRMPTDTRV 283
Score = 76.2 bits (160), Expect(2) = 5e-050
Identities = 32/54 (59%), Positives = 37/54 (68%)
Frame = -1 / +2
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
+ SS S M + AA HAAA + FTIFYNPRA PSEF+IPLAKY KA+Y
Sbjct: 119 MPSSVLSSDSMHLGLLAAAAHAAATNSRFTIFYNPRASPSEFIIPLAKYLKAVY 280
Score = 38.2 bits (77), Expect(2) = 7e-025
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = +3 / -3
Query: 465 IQSLIVFC*RDDKF*WACPWIIENSKWXTICSSMGXCCXDSXXH 596
I S +F R D+F*W PWI++ K CS M + H
Sbjct: 280 INSFKIFSQRYDEF*WTSPWIVKYCKTAICCSCMSCSSKKAQVH 149
>gb|DT475076.1|DT475076 WS0125.BR_O23 PT-GT-FL-A-3 Populus trichocarpa cDNA clone
WS0125_O23 5', mRNA sequence
Length = 578
Score = 149 bits (319), Expect(2) = 4e-047
Identities = 55/73 (75%), Positives = 67/73 (91%)
Frame = -1 / +3
Query: 467 YTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERR 288
+T++S+GMRFRMLFETE+S VRRYMGTITGI DLDP+RW NSHWR+++VGWDESTA ER+
Sbjct: 102 HTRISVGMRFRMLFETEESSVRRYMGTITGISDLDPVRWPNSHWRSVKVGWDESTAGERQ 281
Query: 287 TRVSIWEIEPVAT 249
RVS+WEIEP+ T
Sbjct: 282 PRVSLWEIEPLTT 320
Score = 98.7 bits (209), Expect(2) = 5e-026
Identities = 55/111 (49%), Positives = 62/111 (55%)
Frame = +1 / -2
Query: 154 LKALSTSFSSSGIPACLGSFGLKNGGGHI*NGVATGSISHIETRVRLSDAVDSSQPT*RF 333
L A S S G P C G F + V GSISH +T LS AVDSSQPT
Sbjct: 415 LLASSLLSSKDGDPGCQGRFSRRGNSDGYMGKVVKGSISHNDTLGCLSPAVDSSQPTLTD 236
Query: 334 RQ*EFFHRSGSKSPMPVIVPMYLLTPESSVSKSILNRIPRDTCVYRALLYF 486
RQ E R+GS+S MPVIVPMYL T +SSVSKSI RIP + V L +
Sbjct: 235 RQCELGQRTGSRSLMPVIVPMYLRTLDSSVSKSIRKRIPTEMRV*NTALMY 83
Score = 59.3 bits (123), Expect(2) = 4e-047
Identities = 23/33 (69%), Positives = 28/33 (84%)
Frame = -1 / +3
Query: 563 AAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
AAA + FT+FYNPRA PSEFVIPL+KY KA++
Sbjct: 3 AAATNSCFTVFYNPRASPSEFVIPLSKYIKAVF 101
Score = 38.6 bits (78), Expect(2) = 5e-026
Identities = 17/27 (62%), Positives = 20/27 (74%)
Frame = +1 / -2
Query: 472 ALLYFAKGMTNSDGXALGL*KIVNGLL 552
AL+Y +G+TNSDG ALGL* V LL
Sbjct: 94 ALMYLDRGITNSDGLALGL*NTVKQLL 14
>gb|DT488034.1|DT488034 WS02535.B21_F07 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02535_F07 3', mRNA sequence
Length = 434
Score = 147 bits (316), Expect = 5e-035
Identities = 60/137 (43%), Positives = 82/137 (59%)
Frame = -1 / -2
Query: 620 SSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMR 441
SS S M + A HA + FT++Y PR P+EF++P +Y +++ S+GMR
Sbjct: 412 SSVISSHSMHLGVLATAWHAVSTGTLFTVYYKPRTSPAEFIVPFDQYMESVKNNYSIGMR 233
Query: 440 FRMLFETEDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIE 261
F+M FE E++ +R+ GTI GI D DP RWKNS WR L+V WDE++ R RVS W+IE
Sbjct: 232 FKMRFEGEEAPEQRFTGTIVGIEDADPGRWKNSKWRCLKVRWDETSTMPRPERVSPWKIE 53
Query: 260 PVATPFYICPPPFFRPK 210
P P + P RPK
Sbjct: 52 PALAPPALNPHALPRPK 2
>gb|CN519772.1|CN519772 GQ0106.B3_E05 GQ010 Populus trichocarpa x Populus deltoides cDNA
clone GQ0106_E05 5', mRNA sequence
Length = 779
Score = 146 bits (313), Expect = 1e-034
Identities = 57/124 (45%), Positives = 80/124 (64%)
Frame = -1 / +3
Query: 542 FTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLD 363
FT++Y PR P+EF++P +Y +++ S+GMRF+M FE E++ +R+ GTI GI D D
Sbjct: 12 FTVYYKPRTSPAEFIVPFDQYMESVKNNYSIGMRFKMRFEGEEAPEQRFTGTIVGIEDAD 191
Query: 362 PLRWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVATPFYICPPPFFRPKLPKQAGMPD 183
P RWKNS WR L+V WDE++ R RVS W+IEP P + P P RPK P+ MP
Sbjct: 192 PGRWKNSKWRCLKVRWDETSTMPRPERVSPWKIEPALAPPALNPLPLPRPKRPRANMMPS 371
Query: 182 DENE 171
++
Sbjct: 372 SPDD 383
>gb|DN496803.1|DN496803 Q019A06.5pR Populus dormant bud cDNA library Populus tremula cDNA
clone Q019A06 5', mRNA sequence
Length = 651
Score = 112 bits (239), Expect(2) = 5e-026
Identities = 47/94 (50%), Positives = 59/94 (62%)
Frame = -1 / +3
Query: 578 AAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRR 399
A +A + F I+YNPRA SEF+IP K+ K+L S GMR +M FETED+ RR
Sbjct: 246 ADVANAISMRSAFRIYYNPRASSSEFIIPFNKFLKSLDQSFSSGMRVKMRFETEDAAERR 425
Query: 398 YMGTITGIGDLDPLRWKNSHWRNLQVGWDESTAS 297
Y G ITGI +LDP RW S W+ L V WD+ A+
Sbjct: 426 YTGLITGISELDPTRWPGSKWKCLLVRWDDMEAN 527
Score = 24.9 bits (48), Expect(2) = 5e-026
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = -1 / +3
Query: 299 SERRTRVSIWEIEP 258
+ R +RVS WE+EP
Sbjct: 522 ANRHSRVSPWEVEP 563
>gb|BI138876.1|BI138876 F118P18Y Populus flower cDNA library Populus trichocarpa cDNA, mRNA
sequence
Length = 289
Score = 75.3 bits (158), Expect(2) = 2e-025
Identities = 31/54 (57%), Positives = 36/54 (66%)
Frame = -1 / -3
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
L SS S M + AA HA AN PFTIFYNPRA PS+FVIPL K+ KA++
Sbjct: 263 LPSSVLSADSMHIGVLAAAAHATANRSPFTIFYNPRACPSDFVIPLIKFRKAVF 102
Score = 59.7 bits (124), Expect(2) = 2e-025
Identities = 24/30 (80%), Positives = 27/30 (90%)
Frame = -1 / -3
Query: 464 TQVSLGMRFRMLFETEDSGVRRYMGTITGI 375
TQVS+GMRF M+FETE+SG RRYMGTI GI
Sbjct: 98 TQVSVGMRFGMMFETEESGKRRYMGTIVGI 9
>gb|DT491850.1|DT491850 WS02548.C21_P18 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02548_P18 3', mRNA sequence
Length = 302
Score = 115 bits (246), Expect = 2e-025
Identities = 43/88 (48%), Positives = 61/88 (69%)
Frame = -1 / -1
Query: 542 FTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRRYMGTITGIGDLD 363
FT++Y PR P+EF++P +Y +++ S+GMRF+M FE E++ +R+ GTI GI D D
Sbjct: 266 FTVYYKPRTSPAEFIVPFDQYMESVKNNYSIGMRFKMRFEGEEAPEQRFTGTIVGIEDAD 87
Query: 362 PLRWKNSHWRNLQVGWDESTASERRTRV 279
P RWKNS WR L+V WDE++ R RV
Sbjct: 86 PGRWKNSKWRCLKVRWDETSTMPRPERV 3
>gb|BU870838.1|BU870838 Q019A06 Populus flower cDNA library Populus trichocarpa cDNA 5
prime, mRNA sequence
Length = 684
Score = 112 bits (239), Expect(2) = 4e-025
Identities = 47/94 (50%), Positives = 59/94 (62%)
Frame = -1 / +3
Query: 578 AAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALYTQVSLGMRFRMLFETEDSGVRR 399
A +A + F I+YNPRA SEF+IP K+ K+L S GMR +M FETED+ RR
Sbjct: 222 ADVANAISMRSAFRIYYNPRASSSEFIIPFNKFLKSLDQSFSSGMRVKMRFETEDAAERR 401
Query: 398 YMGTITGIGDLDPLRWKNSHWRNLQVGWDESTAS 297
Y G ITGI +LDP RW S W+ L V WD+ A+
Sbjct: 402 YTGLITGISELDPTRWPGSKWKCLLVRWDDMEAN 503
Score = 22.1 bits (42), Expect(2) = 4e-025
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = -1 / +3
Query: 287 TRVSIWEIEP 258
+RVS WE+EP
Sbjct: 510 SRVSPWEVEP 539
>gb|CA930279.1|CA930279 MTU2CA.P9.E10 Aspen apex cDNA Library Populus tremuloides cDNA,
mRNA sequence
Length = 582
Score = 74.8 bits (157), Expect(2) = 1e-022
Identities = 32/54 (59%), Positives = 36/54 (66%)
Frame = -1 / -1
Query: 626 LSSSXXSXXXMXXXIXAAXXHAAANSXPFTIFYNPRAXPSEFVIPLAKYNKALY 465
+ SS S M + AA HAAA + FTIFYNPRA PSEFVIPL KY KA+Y
Sbjct: 237 MPSSVLSSDSMHIGLLAAAAHAAATNSRFTIFYNPRASPSEFVIPLVKYIKAVY 76
Score = 51.5 bits (106), Expect(2) = 1e-022
Identities = 20/25 (80%), Positives = 24/25 (96%)
Frame = -1 / -1
Query: 467 YTQVSLGMRFRMLFETEDSGVRRYM 393
+T+VS+GMRFRMLFETE+S VRRYM
Sbjct: 75 HTRVSVGMRFRMLFETEESSVRRYM 1
>gb|DT482117.1|DT482117 WS02533.BR_P08 PT-MB-N-A-15 Populus trichocarpa cDNA clone
WS02533_P08 5', mRNA sequence
Length = 662
Score = 105 bits (224), Expect = 3e-022
Identities = 37/57 (64%), Positives = 48/57 (84%)
Frame = -1 / +3
Query: 419 EDSGVRRYMGTITGIGDLDPLRWKNSHWRNLQVGWDESTASERRTRVSIWEIEPVAT 249
+ + + RYMGTITG DLDP+RW NSHWR+++VGWDESTA ER+ RVS+WEIEP+ +
Sbjct: 48 DSTWILRYMGTITGTSDLDPVRWPNSHWRSVKVGWDESTAGERQPRVSLWEIEPLTS 218
Database: Populus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:49 PM
Number of letters in database: 203,408,664
Number of sequences in database: 369,679
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 316,487,206
Number of Sequences: 369679
Number of extensions: 4700842
Number of successful extensions: 61757
Number of sequences better than 1.0e-020: 12
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7685033663
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)