TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCS16e03.yg.2.1
         (608 letters)

Database: Populus_nucl_with_EST.fasta 
           369,679 sequences; 203,408,664 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CX170373.1|CX170373  E02_69-104_10.ab1 leaf inoculated wi...   156   1e-053
gb|CX182279.1|CX182279  G09_45-117_13.ab1 leaf inoculated wi...   115   7e-041

>gb|CX170373.1|CX170373 E02_69-104_10.ab1 leaf inoculated with Marssonia pathogen of
           Populus deltoides Populus deltoides cDNA, mRNA sequence
          Length = 610

 Score =  156 bits (335), Expect(2) = 1e-053
 Identities = 66/99 (66%), Positives = 79/99 (79%)
 Frame = -2 / +2

                                                                       
Query: 385 SEMPFAPIASDSISLIISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISK 206
           S    +P +SD I+LI+SVEDTG GIP +AQ RVFTPFMQV PSI+R +GGTGIGLSISK
Sbjct: 272 SSHTLSPSSSDLINLIVSVEDTGEGIPLEAQPRVFTPFMQVDPSISRKYGGTGIGLSISK 451

                                                  
Query: 205 CLVGLMRGEIGFASKPQVGSTFTFTAVLTRAHSSRNENK 89
           CLVGLM G+IGF S P +GSTFTFTAV +   S+ N++K
Sbjct: 452 CLVGLMNGDIGFVSIPDIGSTFTFTAVFSNGCSNSNDSK 568




 Score =  138 bits (296), Expect(2) = 1e-034
 Identities = 59/95 (62%), Positives = 72/95 (75%)
 Frame = +3 / -2

                                                                       
Query: 90  LFSFLLEWALVRTAVKVKVEPTWGLLANPISPLMSPTKHLLMLNPMPVPPWMRAMDGPTC 269
           L SF LE  L++TAVKVKV+P  G+L  PISP M PTKHLLML PMPVPP+   M+G TC
Sbjct: 567 LESFELEQPLLKTAVKVKVDPISGMLTKPISPFMRPTKHLLMLRPMPVPPYFLEMEGSTC 388

                                              
Query: 270 MKGVKTRD*ASNGIPTPVSSTDIIKLIESDAMGAK 374
           +KGVKTR  AS GIP+PVSST+ I+L+ S+  G +
Sbjct: 387 IKGVKTRGWASRGIPSPVSSTETIRLMRSEEDGER 283




 Score = 73.9 bits (155), Expect(2) = 1e-053
 Identities = 32/69 (46%), Positives = 43/69 (62%)
 Frame = -2 / +2

                                                                       
Query: 592 QIXXXXXXXSXXXTEXXHIYXTVHVVEEIMXXLEXETGTQSANTLSGYPVANRKRSWENF 413
           QI       S   T+  HI+ TVH+VEE+M  ++ ET + S NTLSG PVA+R RSW  F
Sbjct: 71  QIITNLMGNSIKFTKKGHIFLTVHLVEEVMDSIDVETESSSRNTLSGLPVADRCRSWVGF 250

                    
Query: 412 RVFSRELNS 386
           + F+ E +S
Sbjct: 251 KTFNPEGSS 277




 Score = 28.1 bits (55), Expect(2) = 1e-034
 Identities = 14/24 (58%), Positives = 15/24 (62%)
 Frame = +3 / -2

                                   
Query: 402 LKTRKFSQLRFLFATG*PLKVLAD 473
           LK    +QLR L ATG PLKV  D
Sbjct: 261 LKVLNPTQLRHLSATGKPLKVFRD 190



>gb|CX182279.1|CX182279 G09_45-117_13.ab1 leaf inoculated with Marssonia pathogen of
           Populus euramericana Populus x canadensis cDNA, mRNA
           sequence
          Length = 685

 Score =  115 bits (245), Expect(2) = 7e-041
 Identities = 48/66 (72%), Positives = 56/66 (84%)
 Frame = -2 / +1

                                                                       
Query: 385 SEMPFAPIASDSISLIISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISK 206
           S    +P +SD ++LI+SVEDTG GIP +AQ RVFTPFMQV PSI+RI+GGTGIGLSISK
Sbjct: 487 SSHTLSPSSSDLVNLIVSVEDTGEGIPLEAQPRVFTPFMQVDPSISRIYGGTGIGLSISK 666

                 
Query: 205 CLVGLM 188
           CLVGLM
Sbjct: 667 CLVGLM 684




 Score = 98.2 bits (208), Expect(2) = 2e-021
 Identities = 40/60 (66%), Positives = 47/60 (78%)
 Frame = +3 / -3

                                                                       
Query: 195 PTKHLLMLNPMPVPPWMRAMDGPTCMKGVKTRD*ASNGIPTPVSSTDIIKLIESDAMGAK 374
           PTKHLLML PMPVPP++  MDG TC+KGVKTR  AS GIP+PVSSTD I+L  S+  G +
Sbjct: 677 PTKHLLMLRPMPVPPYILEMDGSTCIKGVKTRGWASRGIPSPVSSTDTIRLTRSEEDGER 498




 Score = 72.1 bits (151), Expect(2) = 7e-041
 Identities = 32/69 (46%), Positives = 43/69 (62%)
 Frame = -2 / +1

                                                                       
Query: 592 QIXXXXXXXSXXXTEXXHIYXTVHVVEEIMXXLEXETGTQSANTLSGYPVANRKRSWENF 413
           QI       S   T+  HI+ TVH VEE+M  ++ ET + S NTLSG PVA+R+ S   F
Sbjct: 286 QIITNLMGNSIKFTKKGHIFLTVHPVEEVMDSIDVETESSSLNTLSGLPVADRRTSCGGF 465

                    
Query: 412 RVFSRELNS 386
           ++FSRE +S
Sbjct: 466 KIFSREGSS 492




 Score = 24.0 bits (46), Expect(2) = 2e-021
 Identities = 14/26 (53%), Positives = 15/26 (57%)
 Frame = +3 / -3

                                     
Query: 396 SLLKTRKFSQLRFLFATG*PLKVLAD 473
           S LK     QL  L ATG PLKV +D
Sbjct: 482 SRLKILNPPQLVRLSATGKPLKVFSD 405



  Database: Populus_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:49 PM
  Number of letters in database: 203,408,664
  Number of sequences in database:  369,679
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 269,908,235
Number of Sequences: 369679
Number of extensions: 3985378
Number of successful extensions: 50247
Number of sequences better than 1.0e-020: 2
length of database: 67,802,888
effective HSP length: 51
effective length of database: 48,949,259
effective search space used: 7391338109
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)